White Bars Research Articles

Effect of curing conditions on heat resistance in white chocolate

AbstractHeat‐resistant chocolate is of much interest to confectionery companies for marketing in tropical areas. Methods exist to produce heat‐resistant chocolate by exposing the product to high relative humidity (RH) and increased temperatures. The overall objective of this research project was to explore the curing of white chocolate (30% whole milk powder, 44% sucrose, and 26% cocoa butter) to make it heat resistant and able to be picked up at 33 and 55°C. The curing involved storing solid chocolate samples at 83% RH at 29°C for 1 week. Moisture content before and after curing was measured using the Karl Fischer technique. Force required to penetrate (hardness) was measured at 29°C using a texturometer. Curing samples of white chocolate bars at a lower relative humidity (50% RH at 18°C for 12 weeks or 50% RH at 29°C for 12 weeks) also resulted in a heat‐resistant chocolate that exhibited internal structure, as monitored by SEM. The moisture content in heat‐resistant milk chocolate increased from an average value of 0.84% to 4.6% during the curing process. Curing increased the penetration force, indicating the development of internal structure. This study showed that heat resistance in white chocolate can be achieved by curing solid chocolate samples at controlled humidity and temperature conditions.

Monophyly or Homoplasy? The Relationships of a Rare New Species of Cambeva (Siluriformes: Trichomycteridae) from the Brazilian Atlantic Forest with a Bicolored Caudal Pattern

The Brazilian Atlantic Forest, one the five main biodiversity hotspots in the world, still houses many organisms that are rare and unknown to science, mostly concentrated in mountain ranges. Herein, we describe a rare new species of Cambeva from the Rio Tietê drainage at the Serra da Mantiqueira, exhibiting a caudal fin color pattern comprising a light-yellowish–white bar posteriorly edged by a black pigmented area. A similar color pattern was described for C. castroi, C. diabola, and C. melanoptera, also endemic to the Rio Paraná basin, and considered evidence of close relationships among these three species. However, other morphological characteristics highly suggest that the new species is not closely related to C. castroi, C. diabola, and C. melanoptera). Herein, we perform a molecular phylogenetic analysis using two mitochondrial genes and one nuclear gene, including all species with that bicolored caudal pattern. The analysis strongly supports the new species as a member of the alpha-clade, and therefore as distantly related to those three species belonging to the beta-clade, indicating that this color pattern has homoplastically arisen in Cambeva. The data reported here reinforce the urgent need for field studies to better understand the different evolutionary patterns found in the genus, since an intense process of environmental degradation is in course.

Bayesian analysis on missing visual information and object complexity on visual search for object orientation and object identity.

Missing visual information, such as a gap between an object or an occluded view, has been shown to disrupt visual search and make amodal completion inefficient. Previous research, using simple black bars as stimuli, failed to show a pop-out effect (flat search slope across increasing visual set sizes) during a feature search when the target was partially occluded, but not in cases where it was fully visible. We wanted to see if this lack of a pop-out effect during feature (orientation) search extended to complex objects (Experiment 1) and identity search (Experiment 2). Participants completed orientation and identity visual search tasks by deciding whether the target was present or not present. Bayesian analyses was conducted to find evidence for observed data to be under the null (pop-out effects) or alternative hypotheses (differences in search slopes). When no occluders or gaps were present, a pop-out effect occurred when searching for a simple objects' orientation or identity. In addition, object complexity affected identity search, with anecdotal evidence suggesting that some complex object may not show a pop-out effect. Furthermore, white occluding bars were more disruptive than having a gap of visual information for feature search but not for identity search. Overall, pop-out effects do occur for simple objects, but when the task is more difficult, search for real-world objects is greatly affected by any type of visual disruption.

Problems and Handling Methods of Laser Printers

A laser printer is a common office and personal printing equipment widely welcomed for high-speed, high-quality print output. The high temperature usually causes the printer nozzle temperature to be too high, affecting the printing quality and even damaging the printer. This is because the laser printer works by attaching the toner to the paper through high-temperature sintering, hence the print head’s temperature. Maintaining a suitable temperature environment is crucial for the normal operation and life of the laser printer. In addition, If the position and thickness of the black and white tendons change, it may affect the uneven distribution of the ink when printing, resulting in patches or blur of the printed image. In the printer’s cartridges, the black and white bars are separated, ensuring that the ink in the cartridge is evenly distributed when printing. If the position and thickness of the black and white tendons change, it may affect the uneven distribution of the ink when printing, resulting in patches or blur of the printed image. Therefore, when replacing ink cartridges or resetting or modifying some settings of the printer, be careful to be sure not to damage the printer. This study aims to solve the technical defects of laser printers, improve the level of printing technology, and improve the user experience.

Counting Nemo: anemonefish Amphiprion ocellaris identify species by number of white bars.

The brilliant colors of coral reef fish have received much research attention. This is well exemplified by anemonefish, which have distinct white bar patterns and inhabit host anemones and defend them as a territory. The 28 described species have between 0 and 3 white bars present, which has been suggested to be important for species recognition. In the present study, we found that Amphiprion ocellaris (a species that displays three white bars) hatched and reared in aquaria, when faced with an intruder fish, attacked their own species more frequently than other species of intruding anemonefish. Additionally, we explicitly tested whether this species could distinguish models with different numbers of bars. For this, 120 individuals of A. ocellaris were presented with four different models (no bars, and 1, 2 and 3 bars) and we compared whether the frequency of aggressive behavior towards the model differed according to the number of bars. The frequency of aggressive behavior toward the 3-bar model was the same as against living A. ocellaris, and was higher than towards any of the other models. We conclude that A. ocellaris use the number of white bars as a cue to identify and attack only competitors that might use the same host. We considered this as an important behavior for efficient host defense.

ECR Spotlight – Kina Hayashi

ECR Spotlight is a series of interviews with early-career authors from a selection of papers published in Journal of Experimental Biology and aims to promote not only the diversity of early-career researchers (ECRs) working in experimental biology but also the huge variety of animals and physiological systems that are essential for the ‘comparative’ approach. Kina Hayashi is an author on ‘ Counting Nemo: anemonefish Amphiprion ocellaris identify species by number of white bars’, published in JEB. Kina is a postdoc in the lab of Vincent Laudet at Marine Eco-Evo-Devo Unit, Okinawa Institute of Science and Technology (OIST), Japan, investigating the ecology of anemonefish (population dynamics, community structure, coexistence mechanisms, behavior, etc.) in the Ryukyu Islands.

Attentional guidance from unique faster/slower discrete and smooth feature changes in visual search

ABSTRACT Two studies examine the circumstances in which singletons, defined by unique feature-change rate, guide attention in visual search. Participants searched for a static vertical/horizontal white target bar amidst tilted distractors. Bars were contained inside surround shapes with periodically oscillating features. In Experiment 1, displays consisted of surrounds with abrupt (discrete) or continuous (smooth) changes between two values (red–blue, square–diamond). For discrete displays, target surrounds did not guide attention when uniquely faster-oscillating than distractor surrounds, but did in smooth displays. For unique-slow oscillating target surrounds, the opposite guidance pattern was found across discrete and smooth displays. In Experiment 2, displays had a mixture of discrete and smooth surrounds. Here, only unique slow-oscillating discrete surrounds guided attention. No guidance was found for smooth surrounds. Findings suggest that faster oscillations are masked by higher-frequency harmonic signals from slower changing discrete items, and there is attentional prioritization of discrete over smooth changes.

Effect of wet-heparinized suction on the quality of mediastinal solid tumor specimens obtained by endoscopic ultrasound-guided fine-needle aspiration: a retrospective study from a single center

BackgroundMediastinal lesions are diagnosed sometimes by endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA). Wet-heparinized suction technique has been used to improve the quality of abdominal solid tumor samples obtained by EUS-FNA. The aim of the study is to assess the effect of wet-heparinized suction on the quality of mediastinal solid tumor samples and to evaluate the safety of the method.MethodsThe medical records, EUS-FNA records, pathologic data, and follow-up data between the patients who suspected mediastinal lesions with wet-heparinized suction and conventional suction were retrospectively and comparatively analyzed. Adverse events at 48 h and 1 week after EUS-FNA were evaluated.ResultsWet-heparinized suction contributed to more tissue specimens (P < 0.05), superior tissue integrity (P < 0.05), and a longer length of white tissue core (P < 0.05). In addition, the more complete the tissue bar was, the higher the rate of successful sample (P < 0.05). Moreover, the total length of the white tissue bar at the first puncture was remarkably longer in the Experimental group (P < 0.05). No significant difference in red blood cell contamination in paraffin sections was found between the two groups (P > 0.05). There was no complication after discharge in both groups.ConclusionWet-heparinized suction can improve the quality of mediastinal lesion samples obtained by EUS-FNA and increase the success rate of sampling. In addition, it will not aggravate blood contamination in paraffin sections while ensuring a safe puncture.

Study of the Mexican Cocoa Market: An Analysis of Its Competitiveness (2010–2021)

Cocoa is one of the main products consumed worldwide that is similar to coffee; a primary difference between coffee and cocoa is that cocoa is produced in developing countries and is consumed mainly in industrialized countries. Mexico was the country that made cocoa known to the world. The objective of the study was to analyze the competitiveness at the macroeconomic level of the Mexican cocoa product in the world during the period 2010–2021. A macroeconomic analysis was carried out using six indices: Balassa index, Vollrath Index, Additive Revealed Competitive Advantage Ratio, Trade Openness Index, Export and Import Index, and Self-Sufficiency Index. The industrialization of cocoa is the denaturation of the product, which is launched onto the market with little or no nutritional content, due to the alteration of the raw material with formulas based on vegetable fats, artificial colors, and flavors. For example, a commercial chocolate bar can have only 25–35% cocoa mass, semisweet chocolate bars can vary from 45 to 99% cocoa, while white chocolate bars only contain cocoa butter, milk, and sugar. These facts result in a decrease in the competitiveness of the product in the international market, in addition to the effects produced on its profitability for the producer—an increase in the cost of raw materials and a drop in real profits. These factors generate dependency on the international market for the production.

“Lucky Enough”: The Music and Stories That Make Vaughan's Lounge, New Orleans, Louisiana, United States

“Lucky Enough”: The Music and Stories That Make Vaughan's Lounge, New Orleans, Louisiana, United States

Assessing the Optimal Stage-Cam Target for Continuous Water Level Monitoring in Ephemeral Streams: Experimental Evidence

Recently, increased attention has been devoted to intermittent and ephemeral streams (IRES) due to the recognition of their importance for ecology, hydrology, and biogeochemistry. However, IRES dynamics still demand further research, and traditional monitoring approaches present several limitations in continuously and accurately capturing river network expansion/contraction. Optical-based approaches have shown promise in noninvasively estimating the water level in intermittent streams: a simple setup made up of a wildlife camera and a reference white pole led to estimations within 2cm of accuracy in severe hydrometeorological conditions. In this work, we investigate whether the shortcomings imposed by adverse illumination can be partially mitigated by modifying this simple stage-cam setup. Namely, we estimate the image-based water level by using both the pole and a larger white bar. Further, we compare such results to those obtained with larger bars painted in the red, green, and blue primary colors. Our findings show that using larger white bars also increases reflections and, therefore, the accuracy in the estimation of the water level is not necessarily enhanced. Likewise, experimenting with colored bars does not significantly improve image-based estimations of the stage. Therefore, this work confirms that a simple stage-cam setup may be sufficient to monitor IRES dynamics, suggesting that future efforts may be rather focused on including filters and polarizers in the camera as well as on improving the performance of the image processing algorithm.

Higher ultraviolet skin reflectance signals submissiveness in the anemonefish, Amphiprion akindynos.

Ultraviolet (UV) vision is widespread among teleost fishes, of which many exhibit UV skin colors for communication. However, aside from its role in mate selection, few studies have examined the information UV signaling conveys in other socio-behavioral contexts. Anemonefishes (subfamily, Amphiprioninae) live in a fascinating dominance hierarchy, in which a large female and male dominate over non-breeding subordinates, and body size is the primary cue for dominance. The iconic orange and white bars of anemonefishes are highly UV-reflective, and their color vision is well tuned to perceive the chromatic contrast of skin, which we show here decreases in the amount of UV reflectance with increasing social rank. To test the function of their UV-skin signals, we compared the outcomes of staged contests over dominance between size-matched Barrier Reef anemonefish (Amphiprion akindynos) in aquarium chambers viewed under different UV-absorbing filters. Fish under UV-blocking filters were more likely to win contests, where fish under no-filter or neutral-density filter were more likely to submit. For contests between fish in no-filter and neutral density filter treatments, light treatment had no effect on contest outcome (win/lose). We also show that sub-adults were more aggressive toward smaller juveniles placed under a UV filter than a neutral density filter. Taken together, our results show that UV reflectance or UV contrast in anemonefish can modulate aggression and encode dominant and submissive cues, when changes in overall intensity are controlled for.

Colour patterns influence symbiosis and competition in the anemonefish-host anemone symbiosis system.

Colour patterns in fish are often used as an important medium for communication. Anemonefish, characterized by specific patterns of white bars, inhabit host anemones and defend the area around an anemone as their territory. The host anemone is used not only by the anemonefish, but also by other fish species that use anemones as temporary shelters. Anemonefish may be able to identify potential competitors by their colour patterns. We first examined the colour patterns of fish using host anemones inhabited by Amphiprion ocellaris as shelter and compared them with the patterns of fish using surrounding scleractinian corals. There were no fish with bars sheltering in host anemones, although many fish with bars were found in surrounding corals. Next, two fish models, one with white bars and the other with white stripes on a black background, were presented to an A. ocellaris colony. The duration of aggressive behaviour towards the bar model was significantly longer than that towards the stripe model. We conclude that differences in aggressive behaviour by the anemonefish possibly select the colour patterns of cohabiting fish. This study indicates that colour patterns may influence not only intraspecific interactions but also interspecific interactions in coral reef ecosystems.

Divas on the Dance Floor

Divas on the Dance Floor

Effect of the consumption of a cocoa paste bar (Theobroma cacao L.) on metabolic indicators in university students

The aim of this study was to determine the effect on metabolic indicators (total cholesterol, triacylglycerides, HDL, LDL and glycemia) in university students, after the daily consumption of 1.00 g of a cocoa paste bar (PCB) and 0.50 g of a white chocolate bar (BCB) during two months. The population was characterized according to their sex, weight, height, body mass index (BMI), physical activity and diet. The pre- and post-metabolic levels were measured in the control group (BCB) and experimental group (BPC). The students have normal weight (22.90 kg/m2 and 24.99 kg/m2), average physical activity was predominat (55.60 %) and their nutrition was inadequate. The study groups presented metabolic levels within the normal parameter. However, the consumption of 1 g of PCB for two months increases HDL levels (36.94 ± 13.42 mg/dL vs 59.74 ± 9.41 mg/dL), an effect that is highly desirable in a food

To “Throw Off the Cloak of Representation”

To “Throw Off the Cloak of Representation”

Abstract 114: The Epigenetic Enzyme KMT2A/MLL1 Is A Driver Of Coronavirus Associated Coagulopathy

Objectives: Coronavirus associated coagulopathy (CAC) is postulated to be driven by systemic macrophage activation after SARS-CoV-2 infection and presents with elevated risk of thrombogenesis and hyperfibrinolysis. Previous work shows that the histone methyltransferase KMT2A/MLL1 is a key mediator of inflammatory signaling in monocytes and macrophages (Mo/Mϕs). In this study, we sought to identify the regulation of factors important in CAC by MLL1. Methods: Mice with myeloid specific knockout of MLL1 (Cre+) and littermate controls (Cre-) underwent intranasal inoculation of 2 x 10 5 pfu of the murine coronavirus MHVA59, an established model which phenocopies SARS-CoV-2 infection. Splenic Mϕs (surrogate for circulating Mo/Mϕs) were isolated and RNA and protein levels of urokinase (Plau; profibrinolytic), urokinase receptor (Plaur; profibrinolytic), and tissue factor (F3/TF; procoagulant) were analyzed using qRT-PCR and ELISA, respectively. Thromboelastography (TEG) on whole blood and urokinase activity assays from mouse plasma were performed. Urokinase and TF activity assays were performed on plasma from human samples. Results: RNA (top panel) and protein (bottom) levels of Plau, Plaur, and F3 were suppressed in the Splenic Mϕs harvested from sham (intranasal PBS) and infected Cre+ animals (white bars) compared to Splenic Mϕs harvested from Cre- animals (blue bars; Fig. 1A). Cre- mice displayed a shortened R-time (reaction time) as measured by TEG (Fig. 1B) and elevated plasma urokinase activity levels (not shown). Hospitalized COVID-positive patients (hCOV+) displayed elevated plasma urokinase and TF activity levels (Fig. 1C). Conclusions: We identify a role for MLL1 for basal expression and for coronavirus-mediated induction of factors important for fibrinolysis and coagulation in murine Mo/Mϕs and in driving coagulopathy. Our results suggest that MLL1 blockade may be an attractive strategy to combat coronavirus associated coagulopathy.

IRE-dependent Regulation of Intestinal Dmt1 Prevails During Chronic Dietary Iron Deficiency but is Dispensable in Conditions of Acute Erythropoietic Stress.

IRE-dependent Regulation of Intestinal Dmt1 Prevails During Chronic Dietary Iron Deficiency but is Dispensable in Conditions of Acute Erythropoietic Stress.

Development of Human Gut Organoids With Resident Tissue Macrophages as a Model of Intestinal Immune Responses.

Development of Human Gut Organoids With Resident Tissue Macrophages as a Model of Intestinal Immune Responses.

Basic Graphic Shape Decoding for EEG-based Brain-Computer Interfaces.

Image decoding using electroencephalogram (EEG) has became a new topic for brain-computer interface (BCI) studies in recent years. Previous studies often tried to decode EEG signals modulated by a picture of complex object. However, it's still unclear how a simple image with different positions and orientations influence the EEG signals. To this end, this study used a same white bar with eight different spatial patterns as visual stimuli. Convolutional neural network (CNN) combined with long short-term memory (LSTM) was employed to decode the corresponding EEG signals. Four subjects were recruited in this study. As a result, the highest binary classification accuracy could reach 97.2%, 95.7%, 90.2%, and 88.3% for the four subjects, respectively. Almost all subjects could achieve more than 70% for 4-class classification. The results demonstrate basic graphic shapes are decodable from EEG signals, which hold promise for image decoding of EEG-based BCIs.