Herein, we extracted proteins from four microalgae: Chlamydomonas reinhardtii (CHR), Euglena gracilis (EUG), Spirulina platensis (SPP), and Spirulina maxima (SPM). Subsequently, their physicochemical and functional properties, as well as air-water interface adsorption behavior were investigated. Results demonstrated that the solubility and emulsifying properties of these proteins were significantly affected by pH, with minimum values observed near their isoelectric point (3.5) and maximum values at pH 11.0. Interestingly, the emulsifying properties of the four proteins were superior to soy protein isolate in the pH range of 3.0–7.0, indicating that these microalgae proteins may be suitable for applying as emulsifiers in foods. EUG exhibited the highest water/oil binding capacity, emulsifying activity, and emulsifying stability, which were attributed to its high surface hydrophobicity and β-sheet content, as well as small particle size. Dynamic adsorption behavior analysis illustrated that EUG showed faster diffusion and rearrangement rates, as well as a higher final equilibrated surface pressure value, contributing to its highest foaming capacity and foaming stability. This study greatly enhances the understanding of microalgae proteins and is expected to establish a theoretical foundation for developing novel protein resources that can be utilized in the foamed and emulsion-based food fabrication, including whipped cream and functional beverages.
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