FVC (fractional vegetation cover) is highly correlated with wheat plant density in the reviving period, which is an important indicator for conducting variable-rate nitrogenous topdressing. In this study, with the objective of improving inversion accuracy of wheat plant density, an innovative approach of retrieval of FVC values from remote sensing images of a UAV (unmanned aerial vehicle) was proposed based on the mixed pixel decomposition method. Firstly, remote sensing images of an experimental wheat field were acquired by using a DJI Mini UAV and endmembers in the image were identified. Subsequently, a linear unmixing model was used to subdivide mixed pixels into components of vegetation and soil, and an abundance map of vegetation was acquired. Based on the abundance map of vegetation, FVC was calculated. Consequently, a linear regression model between the ground truth data of wheat plant density and FVC was established. The coefficient of determination (R2), RMSE (root mean square error), and RRMSE (Relative-RMSE) of the inversion model were calculated as 0.97, 1.86 plants/m2, and 0.677%, which indicates strong correlation between the FVC of mixed pixel decomposition method and wheat plant density. Therefore, we can conclude that the mixed pixel decomposition model of the remote sensing image of a UAV significantly improved the inversion accuracy of wheat plant density from FVC values, which provides method support and basic data for variable-rate nitrogenous fertilization in the wheat reviving period in the manner of precision agriculture.