Wheat Lines Research Articles

Physiological phenotyping of transpiration response to vapour pressure deficit in wheat

BackgroundPrecision phenotyping of short-term transpiration response to environmental conditions and transpiration patterns throughout wheat development enables a better understanding of specific trait compositions that lead to improved transpiration efficiency. Transpiration and related traits were evaluated in a set of 79 winter wheat lines using the custom-built “DroughtSpotter XXL” facility. The 120 l plant growth containers implemented in this phenotyping platform enable gravimetric quantification of water use in real-time under semi-controlled, yet field-like conditions across the entire crop life cycle.ResultsThe resulting high-resolution data enabled identification of significant developmental stage-specific variation for genotype rankings in transpiration efficiency. In addition, for all examined genotypes we identified the genotype-specific breakpoint in transpiration in response to increasing vapour pressure deficit, with breakpoints ranging between 2.75 and 4.1 kPa.ConclusionContinuous monitoring of transpiration efficiency and diurnal transpiration patterns enables identification of hidden, heritable genotypic variation for transpiration traits relevant for wheat under drought stress. Since the unique experimental setup mimics field-like growth conditions, the results of this study have good transferability to field conditions.

Development and Characterization of Two Wheat–Rye Introgression Lines with Resistance to Stripe Rust and Powdery Mildew

Rye (Secale cereale L.) genes, which contribute to the tertiary gene pool of wheat, include multiple disease resistance genes useful for the genetic improvement of wheat. Introgression lines are the most valuable materials for wheat breeding because of their small alien segments and limited or lack of linkage drag. In the present study, wheat–rye derivative lines SN21627-2 and SN21627-6 were produced via distant hybridization. A genomic in situ hybridization analysis revealed that SN21627-2 and SN21627-6 lack alien segments, while a multi-color fluorescence in situ hybridization analysis detected structural changes in both introgression lines. At the seedling and adult plant stages, SN21627-2 and SN21627-6 were highly resistant to stripe rust and powdery mildew. Primers for 86 PCR-based landmark unique gene markers and 345 rye-specific SLAF markers were used to amplify SN21627-2 and SN21627-6 genomic DNA. Eight markers specific to rye chromosome 2R were detected in both introgression lines, implying these lines carry chromosome 2R segments with genes conferring stripe rust and powdery mildew resistance. Therefore, SN21627-2 and SN21627-6 are resistant to more than one major wheat disease, making them promising bridging parents for breeding disease-resistant wheat lines.

Identification of Resistance to <i>Puccinia striiformisf</i>. sp. <i>tritici</i> (<i>Pst</i>) in Some Commercial and Elite Bread Wheat Lines

Stripe rust is caused by <i>Puccinia striiformisf</i>. sp. <i>tritici</i> (<i>Pst</i>) threatening wheat production in Ethiopia. The emerged virulent stripe rust races at one point of the world spread to the rest of wheat-producing countries by the wind as well as human travels and damaged popular resistant wheat cultivars thereby posing food insecurity. However, wheat cultivars succumb to new Pst race (s) soon after their release from research centers. This study aimed to determine stripe rust resistance in some Ethiopian commercial and elite bread wheat lines. In 2017, a total of 37 commercial and elite bread wheat lines were exposed to stripe rust in under the disease hot spot areas (Kulumsa and Meraro) Arsi zone Oromia regional state. In the second year (2018), 22 promising lines consisting of 16 commercial bread wheat and 6 elite’s lines were evaluated both at seedling and adult plant growth stages. The seedling test was conducted in the greenhouse at Kulumsa research center using three Pst races. In field evaluations, terminal severity (TRS), coefficient of infection (CI), area under disease progress curve (AUDPC), disease progress rate (DPR), and spike infection (SI) were considered. Of the 37 commercial and elite bread wheat lines, 19 (51.4%) exhibited lower or equal disease reaction compared to the resistant check (Enjoy) across locations and seasons. Eleven bread wheat lines showed both adult plant and seedling resistance. The 37 commercial wheat lines that showed field resistance was further exposed to three Pst races at the seedling stage and 11 exhibited seedling resistance to all races. This study has identified seedling and adult plant resistance in some commercial and elite bread wheat lines to the prevailing Pst races.

Variability of Agronomic Traits of Wheat (Triticum aestivum L.) Lines in Two Agroecosystems in Indonesia

Wheat is an important agricultural commodity in Indonesia, yet the country remains heavily reliant on imports to meet domestic demand. The diverse agroclimatic conditions in the highlands and lowlands pose significant challenges for national wheat production. This study aimed to evaluate the performance of wheat lines in two distinct altitude environments: Cipanas (1120 m above sea level) and Cisarua (600 m above sea level). An augmented randomized complete block design (RCBD) assessed 50 F6 wheat lines derived from the HP1744/”Selayar” cross. The results revealed significant variability in agronomic traits attributable to altitude differences. However, genotype-by-environment (GxE) interactions were found to be non-significant for all evaluated characters. Broad-sense heritability was classified as medium for the grain-filling period in Cipanas and seed weight of the main panicle in Cisarua, while all other traits exhibited low heritability. Based on the stress sensitivity index (SSI), 14 lines (28%) were identified as tolerant to higher temperatures in the medium-altitude environment, particularly in terms of seed weight of the main panicle. These findings are of utmost importance as they underscore the need to consider genetic and environmental factors in developing wheat cultivars suitable for Indonesia’s diverse agro-climatic conditions.

Genome-wide mapping of quantitative trait loci conferring resistance to stripe rust in spring wheat line PI 660072.

Two major QTL for resistance to stripe rust were mapped on chromosome 2BL and 4BL in spring wheat PI 660072, and their KASP markers were developed. Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most devastating diseases of wheat worldwide. Identifying resistance genes is crucial for developing resistant cultivars to control the disease. Spring wheat PI 660072 (Triticum aestivum) has been identified to possess both adult-plant resistance (APR) and all-stage resistance (ASR) to stripe rust. To elucidate the genetic basis of the resistance in PI 660072, a mapping population consisting of 211 F5-F7 recombinant-inbred lines (RILs) was developed from a cross of PI 660072 with susceptible spring wheat Avocet S. The mapping population was phenotyped for stripe rust responses across five field environments from 2020 to 2022 and genotyped using the 15K SNP (single nucleotide polymorphism) array to map stripe rust resistance loci. The mapping population was also tested at the seedling stage with predominant Chinese Pst races CYR31, CYR32, CYR34 and PST-YX1-3-1 in the greenhouse. Stripe rust resistance genes were identified using the quantitative trait locus (QTL) mapping approach. Two QTL were identified with QYrPI660072.swust-2BL mapped on the long arm of chromosome 2B for ASR and QYrPI660072.swust-4BL on the long arm of chromosome 4B for APR. To facilitate marker-assisted selection breeding, Kompetitive allele specific PCR (KASP) markers, KASP-1269 for QYrPI660072.swust-2BL and KASP-3209 for QYrPI660072.swust-4BL, were developed. These markers could be used to introgress the effective resistance QTL into new wheat cultivars.

Virulence characterization of wheat stripe rust population in China in 2023

AbstractWheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), significantly impacts global wheat production, including China. Monitoring Pst virulence is crucial for disease management. This study used 18 Yr single‐gene isogenic lines of wheat to characterize the virulence of 2023 Pst collections in China. A total of 103 isolates were collected from 11 provinces, and 61 races were identified. Frequencies of virulence to the 18 Yr genes varied from 0% to 95.1%, with low frequencies (defined as less than 10%) to Yr85 (7.8%) and Yr10 (8.7%); moderate frequencies (10%–40%) to Yr24 (15.5%), Yr32 (16.5%), YrSP (37.9%) and Yr76 (37.9%); and high frequencies (>40%) to Yr7 (47.6%), Yr1 (68.0%), Yr9 (69.9%), Yr27 (69.9%), Yr17 (79.6%), Yr6 (89.3%), YrExp2 (92.2%), Yr43 (93.2%), Yr8 (95.1%) and Yr44 (95.1%). Yr6, Yr8, Yr17, Yr27, Yr43, Yr44 and YrExp2 have almost lost their effectiveness in all provinces, while Yr10, Yr24, Yr32 and Yr85 are effective in Hebei, Shandong, Shaanxi, Gansu and Anhui. No isolate was virulent to Yr5 or Yr15, indicating their effectiveness against Pst populations in China. The Pst isolates had a wide virulence spectrum ranging from 3 to 16 virulence factors, with an average of 9.5. Comparing 2023 to 2021, the PstCN‐040 and races sharing virulence to Yr1, Yr6, Yr7, Yr8, Yr9, Yr17, Yr43, Yr44 and YrExp2 predominated, with frequencies 16.4% and 25.2%. This study provides valuable information on the virulence composition of Pst populations in China, helping to guide the development of genetic resistance for wheat in China.

The use of the species Triticum petropavlovskyi Udacz. et Migusch. to expand the genetic diversity of spring bread wheat

Background. The species Triticum petropavlovskyi Udacz. et Migusch. has a number of positive traits, but has rarely been used in breeding programs to improve bread wheat cultivars. The introgression of genetic material from this species into the Triticum aestivum L. gene pool will not only expand the genetic diversity of bread wheat with a set of traits valuable for breeders, but also help to analyze the expression of genes from T. petropavlovskyi in a new genetic environment. Materials and methods. Spring bread wheat lines L163 and L164 produced with the participation of bread wheat cv. ‘Voevoda’ and an accession of T. petropavlovskyi were target materials of the study. Conventional methods were applied to perform phenological, phytopathological, genetic, and bread quality evaluations. Statistical processing of the resulting data was carried out using the Agros-2.10 software. Results. L163 and L164 were studied for their morphological and phenological indicators, resistance to pathogens, productivity, and grain quality. Differences in a number of traits between the lines and the recipient cultivar were observed. The introgression of the T. petropavlovskyi genetic material into bread wheat showed both positive and negative effects on some agronomic characteristics. L163 was identified for its resistance to the pathogens of leaf rust and powdery mildew, and tolerance to cereal aphids. It combined high productivity with an increased grain protein content compared to the recipient cultivar. In addition, this line demonstrated good rheological properties of its dough. Conclusion. Merging genetic materials from T. petropavlovskyi and bread wheat cv. ‘Voevoda’ made it possible to produce lines with a set of positive characteristics. The selected line, L163, combined effective resistance to a number of diseases, high grain productivity, and good breadmaking qualities, so it was included in the breeding process.

A major falling number locus on chromosome 4B governs resistance to pre-harvest sprouting in bread wheat line Soru#1

Pre-harvest sprouting (PHS) is a serious threat to wheat quality and occurs when maturing seeds on plants encounter substantial rain before harvest. In the present study, the genetics of PHS resistance were studied using a population of 131 recombinant inbred lines (RILs) from a cross between Soru#1 and Naxos. The population was genotyped with the Illumina 90 K and Axiom 35 K wheat SNP arrays and tested in field trials across two locations in Norway and one in China. Seed dormancy as a measure of sprouting damage was evaluated using germination index (GI) and falling number (FN). Days to heading, days to maturity, and plant height were also recorded to evaluate their potential impact on PHS. Thirteen PHS-related quantitative trait loci (QTL) were detected. The most consistent QTL for FN, contributed by Soru#1, on the long arm of chromosome 4B explained 10.0–19.2% of the phenotypic variation across trials. Other important PHS resistance QTL from Soru#1 detected on chromosome arms 2AL, 2BL, and 4BS, affected both GI and FN. Naxos carried favorable QTL alleles for PHS resistance on chromosome arm 4AS and the Phs1 allele on 4AL. The reduced height Rht-D1 and vernalization Vrn-A1 alleles were the major determinants of plant height and earliness; however, these traits had minimal impact on the genetic control of PHS resistance in this population. Thus, both parents contributed valuable alleles for improving PHS resistance in breeding programs.

Differences in the Virulence Between Local Populations of Puccinia striiformis f. sp. tritici in Southwest China.

The virulence analysis of Puccinia stiiformis f. sp. tritici (Pst), the cause of wheat stripe rust, is essential for predicting and managing the disease epidemic in Southwest China, where the wheat cultivation has significantly reduced in the past few decades due to the impact of this disease. From 2020 to 2021, 196 Pst isolates were collected from Guizhou, Yunnan, and Sichuan. The virulence and race assessments were conducted using Chinese differential genotypes. Additionally, the resistance expression of 102 wheat lines was evaluated in 2021 in two disease nurseries located in Ningnan and Jiangyou. All the 45 Pst isolates from Guizhou and Yunnan belonged to pathogroup Hybrid 46, with 36 identified as race CYR32. Among the 69 isolates from the Liangshan Prefecture, 67 belonged to the Hybrid 46 group, while the remaining two were identified as race CYR34 in the G-22 group. Furthermore, all 79 isolates from the western Sichuan Basin belonged to the G-22 group, with 54 identified as race CYR34. The diversity indices of the Pst populations from Guizhou, Sichuan, and Yunnan exhibited a sequential decline. Virulence variation among the Pst populations from Yunnan, Guizhou, and the Ganzi-Liangshan region was minimal; however, significant virulence differences were observed when these populations were compared to those from the western Sichuan Basin. Results from disease nurseries indicated that Pst virulence was notably stronger in Ningnan compared to that in Jiangyou. The Sichuan Basin exhibits a notable diversity in Pst virulence, coupled with a more frequent genetic exchange occurring between the Liangshan Prefecture and the Yunnan-Guizhou Plateau. This information is essential for developing effective management strategies to mitigate the impact of wheat stripe rust in this region.

Variability of protein and gluten content in bread wheat lines with introgressions into chromosome 5B from related species

Variability of protein and gluten content in bread wheat lines with introgressions into chromosome 5B from related species

The first assessment of grain yield and associated traits in durum wheat across a decade in Nepal.

Rapid urbanization and evolving dietary preferences have heightened the demand for durum wheat and its derivatives in developing nations like Nepal. This study represents the first comprehensive exploration and evaluation of durum wheat genotypes in Nepal, addressing the escalating need for high-yielding varieties. The primary objective was to identify stable and prolific durum wheat lines for release, enhancing Nepal's durum wheat breeding program. Utilizing genotypes from CIMMYT's disease screening and yield nurseries from 2011/12 to 2020/21, a total of 132 genotypes, including international checks, underwent evaluation over ten years under the Alpha Lattice design. Results revealed significant variation among genotypes for grain yield and other traits, identifying high-yielding and stable lines suitable for Nepal. Heritability analysis highlighted moderate heritability for grain number per spike, thousand-grain weight, and grain yield. Cluster analysis identified distinct clusters with high grain yield and desirable agronomic traits. Disease incidence facilitated the selection of resistant lines, with DWK38 emerging as the highest grain yielder (4416.04 kg/ha). Overall, durum wheat lines from CIMMYT exhibited robust performance in Nepal, enabling the identification of superior lines with potential benefits for farmers and consumers. The study's implications include developing and releasing superior durum lines in Nepal, providing farmers with profitable alternatives amidst evolving food habits. In conclusion, the findings from this study provide a valuable foundation for future durum wheat breeding efforts in Nepal, guiding the selection of genotypes that are well-suited to the diverse environmental challenges of the region.

Accelerating genetic gain through strategic speed breeding in spring wheat

AbstractSpring wheat (Triticum aestivum L.) is a popular bread wheat with high milling and baking requirements. Vernalization is not required for spring wheat, which allows for fast growth under manipulated conditions. This experiment's objective was rapid development of inbred lines of hard red spring wheat throughout the off‐season and preserve enough genetic variability to perform selection. A total of 1575 F2 heads from three distinct populations were randomly harvested in the field‐season 2021. To break seed dormancy, seeds were held for 2 days at 0°C. Three breeding cycles were performed through single seed descent under manipulated growth condition during the off‐season 2021–2022. We were able to harvest plant materials as quickly as 54 days after planting in comparison to 110 days average field season. We lost a total of 36.4% during the three off‐season fast advancement generations and 711 genotypes reached the F5:6 generation with enough seed to perform a partially replicated small plot yield trial at two locations during the 2022 field‐season. Response traits collected included grain yield, grain protein, plant height, days to heading, and bacterial leaf streak (Xanthomonas transluens) disease severity. Heritability of collected traits varied between 0.61 and 0.92. Although we had considerable loss during the speed breeding, we were able to identify superior genotypes compared to the parents.

Assessment of genetic variability, heritability and correlation in advanced breeding lines of bread wheat (Triticum aestivum) under heat stress condition

Abstract An experiment was conducted under normal (with six irrigation during crop period) and stressed condition during Rabi 2019 in All India Coordinated Research Project (AICRP) on Wheat, MARS, Dharwad, Karnataka with the view of assessing genetic variability, heritability and expected genetic advance in 104 F4 families of the cross HD2864 × DBW14. The progenies exhibited wide range of variation for all the traits indicating the presence of enough genetic variability in the material under study. Under timely sown condition, the traits like leaf waxyness, NDVI at different stages, SPAD at different stages, number of productive tillers per plant, seeds per spike, grain filling duration, spike length, thousand grains weight and seed set percentage showed high variability. The traits like plant height, canopy temperature at different stages and membrane stability index showed low variability. Under late sown condition, the traits like leaf waxyness, plant height, NDVI at different stages, SPAD at different stages, number of productive tillers per plant, seeds per spike, yield per plant, row bulk yield and seed set percentage showed high variability. The traits like days to maturity, spike length and membrane stability index showed low variability. The findings shows that all traits related to crop yield are positively linked, except for canopy temperature at different growth stages. Promising lines identified through Heat susceptibility index (HSI) and Heat tolerance index (HTI) can be utilized in future breeding programs to enhance crop productivity. Keywords: Variability, Coefficient of variation, Heritability, Genetic advance.

Structural, Molecular, and Physicochemical Properties of Starch in High-Amylose Durum Wheat Lines

Structural, Molecular, and Physicochemical Properties of Starch in High-Amylose Durum Wheat Lines

Exploring the rhizosphere of perennial wheat: potential for plant growth promotion and biocontrol applications

Perennial grains, which remain productive for multiple years, rather than growing for only one season before harvest, have deep, dense root systems that can support a richness of beneficial microorganisms, which are mostly underexplored. In this work we isolated forty-three bacterial strains associated with the rhizosphere of the OK72 perennial wheat line, developed from a cross between winter common wheat and Thinopyrum ponticum. Identified using 16S rDNA sequencing, these bacteria were assessed for plant growth-promoting traits such as indole-3-acetic acid, siderophores and ACC-deaminase acid production, biofilm formation, and the ability to solubilize phosphate and proteins. Twenty-five strains exhibiting in vitro significant plant growth promoting traits, belong to wheat keystone genera Pseudomonas, Microbacterium, Variovorax, Pedobacter, Dyadobacter, Plantibacter, and Flavobacterium. Seven strains, including Aeromicrobium and Okibacterium genera, were able to promote root growth in a commercial annual wheat cultivar while strains from Pseudomonas genus inhibited the growth of Aspergillus flavus and Fusarium species, using direct antagonism assays. The same strains produced a high amount of 1-undecanol a volatile organic compound, which may aid in suppressing fungal growth. The study highlights the potential of these bacteria to form new commercial consortia, enhancing the health and productivity of annual wheat crops within sustainable agricultural practices.

Mapping of resistance loci in wheat line Milan/S87230//Babax to South African races of Puccinia striiformis f. sp. tritici

Mapping of resistance loci in wheat line Milan/S87230//Babax to South African races of Puccinia striiformis f. sp. tritici

Performance of durum wheat cultivars and advanced lines to black point under natural conditions in two crop seasons

Twenty seven advanced durum wheat lines and cultivars CIRNO C2008, Baroyeca Oro C2013, and Quetchehueca Oro C2013 were evaluated for their reaction to black point, under natural conditions at the Norman E. Borlaug Experimental Station, in the Yaqui Valley, Sonora, Mexico, during crop seasons 2015-2016 and 2016-2017. Sowing dates were November 12 and 19, 2015, for the first season, and November 17 and 24, 2016 for the second one, using 8 g of seed for a 0.7 m bed with two rows without replications. Harvest was done manually and the evaluation by visual inspection counting the number of healthy and infected grains in ten spikes per line/cultivar, in order to determine the percentage of infection. Based on the average of the two dates, in the first season one line did not show any infected grain, ten fell within the 0.1-2.5 % infection category, six within 2.6-5.0 %, three within 5.1-10.0 %, and seven within 10.1-30 %. Quetchehueca Oro C2013 did not show any infected grain, while CIRNO C2008 and Baroyeca Oro C2013 showed 1.7 and 12.3 % infection, respectively. In the second season, two lines did not show infected grains, seventeen lines and the three cultivars with 2.4, 1.5, and 1.0 %, respectively, fell within the 0.1-2.5 % infection category, six within 2.6-5.0 %, and two within 5.1-10.0 %. Lines with the highest percentage of infection were: 1A.1D5+1-06/3*MOJO//RCOL/4/ ARMENT//SRN_3/NIGRIS_4/3/CANELO_9.1/5/CF4-JS40//SOOTY_9/RASCON_37/4/CNDO/PRIMADUR//HAI-OU_17 /3/SNITAN/9/CBC509CHILE/6/ECO/CMH76A.722//BIT/3/ALTAR84/4/AJAIA_2/5/KJOVE_1/7/AJAIA_12/ F3LOCAL(SEL.ETHIO.135.85)//PLATA_13/8/S with 50.4 % in the first date of the first season, and WBDTBO/10/ PLATA_10/6/MQUE/4/USDA573//QFN/AA_7/3/ALBA-D/5/AVO/HUI/7/PLATA_13/8/THKNEE_11/9/CHEN/ ALTAR84/3/HUI/POC//BUB/RUFO/4/FNFOOT/11/MÂALI/10/ALTAR84/CMH82A.1062//ALTAR 84/3/YAZI_10/4/ SNITAN/9/USDA595/3/D67.3/RABI//CRA/4/ ALO/5/HUI/YAV_1/6/ARDENTE with 16.3 % in the second date of the second season. Higher infection was detected in crop season 2015-2016 possibly to the occurrence of higher relative humidity during grain expansion.

Effects of ethyl methanesulfonate on growth and yield parameters of wheat and tolerance to imazamox

In the present study objective bread wheat (Triticum aestivum L.) and durum wheat (Triticum durum Desf.) lines resistant to Imazamox herbicides and effective lethal ethyl methanesulfonate (EMS) doses for plants. During first year (2019/2020), 1000 seeds of bread wheat and durum wheat treated with 11 different EMS doses were sown under field and greenhouse conditions. During second year (2020/2021), M1 (first generation) cereal seeds were grown as M2 (second generation) in field experiments. Herbicide with Imazamox active ingredient at 125 ml da-1 was applied to the approximately 15 cm tall plants in the M2 generation. In bread and durum wheat lines, emergent plants were observed up to a dose of 60mM EMS. The effective EMS doses for the wheat species were determined as 80 mM for bread wheat and 50 mM for durum wheat. Bangladesh J. Bot. 53(3): 487-493, 2024 (September)

Androgenesis in vitro in anther culture of bread winter wheat

Aim. Study of the effectiveness of stages of in vitro androgenesis in anther culture for the creation of dihaploid lines of bread winter wheat. Methods. In vitro culture of isolated wheat anthers. For each genotype, the following parameters of androgenesis in vitro were determined: the percentage of caluses and the percentage of regeneration of green plants from the number of planted anthers; the percentage of acclimatized plants from regenerated green plantlets, as well as the percentage of fertile (doubled haploid) plants. Results. Androgenesis in vitro in the anther culture of 105 genotypes of soft winter wheat of different genetic origin was studied. Differences regarding the frequency of callusogenesis induction (from 0.17 to 21.25 percent of planted anthers) and the ability to regenerate plants (from 0 to 4.94 percent of planted anthers) were found. The dihaploid lines in anther culturefor almost 50 % of the studied genotypes were obtained. Spontaneous diploidization averaged 29.41 % of the obtained regenerants. Conclusions. Genotype-specific morphogenetic reactions of bread winter wheat microspores in the process of in vitro androgenesis were revealed. 122 dihaploid lines of bread winter wheat were obtained.

Selection of marker systems for determining the allelic composition of high molecular glutenin loci in winter wheat

Aim. Selection of marker systems and conducting multiplex polymerase chain reactions to determine the allelic composition of the Glu-A1, Glu-B1 and Glu-D1 loci, which determine the high quality of flour in common wheat samples. Methods. Polymerase chain reaction (PCR) using specific primers for the Glu-A1, Glu-B1 and Glu-D1 loci of wheat. Results. PCR analysis was performed to detect the insertion at 43 b. p. in the MAR region, in varieties with the Glu-B1al allele, the presence of an amplicon 563 b. p. long was observed, for varieties that carry other alleles of the locus – an amplicon 520 b. p. long. The results on the Glu-D1 locus showed that for № 2, № 3, № 4, № 7, № 8, № 10, № 11, № 19, № 20, № 21 subunits (5 + 10) were identified. Conclusions. With the help of allele-specific primers, the allelic composition of loci of high molecular weight glutenins was determined in 22 lines wheat. Three alleles Glu-A1a, Glu-A1b, Glu-A1c were found in the Glu-A1 locus; in the Glu-B1 locus, only the Glu-B1al allele was detected, there are two alleles at the Glu-D1 locus: Glu-D1a and Glu-D1d (the Glu-D1a allele predominated). As a result of our research, samples of soft wheat (lines № 2, № 7, № 21) were selected, which can be attributed to genotypes with high baking quality of the flour.