Job’s tears (Coix lacryma-jobi L) is one of staple crop has a potential in improving food security by substituting rice. It is rich of nutrition and essential chemicals compounds, either as food or herbal. Product yield of job’s tear and quality need to be increased for obtaining flour for various food. This study aimed to investigate the physical and chemical properties of Job’s tear flour was subjected by wet milling and dry milling techniques. The result showed that wet-milled flour had higher value of moisture content compare to the dry-milled flour. Thermal properties of wet- and dry-mill were different each other. Conclusion temperature (Tc) - onset temperature (To) of dry-milled flour was statistically higher than wet-mill flour. The gelatinization enthalpy of wet-milled flour (11.2085 J/g) was higher than dry-milled flour samples (7.8542J/g). Dry milling technique caused more damaged and had more various cracks, fissures and holes on the starch surface than wet.