Wet Distillers Grains Plus Solubles Research Articles

Effect of Using Ensilaged Corn Wet Distillers’ Grains Plus Solubles (WDGS) as a Partial Replacement for Concentrated Feed for Wet Lot Fed Fatteners during Fattening on Growth Performance, Carcass Characteristics and Pork Quality

The purpose of this study was to determine the nutritional suitability of WDGS in pigs’ feeding and production. Pigs were liquid fed and divided into 3 groups. Pigs in the control group were fed diets based on cereal grains, while the experimental groups were also given 10% or 15% WDGS, which partially replaced their cereal grains. During this study, the average daily gains (ADG), feed intake, chemical composition of meat, fatty acid profile of meat, and quality parameters of the carcass and meat were examined. The highest statistical weight gains were detected for the group WDGS 10% during the first stage of the fattening period. No statistical differences were detected for the final body weight, carcass traits, chemical composition of the meat or the composition of fatty acids such as SFAs, PUFAs, and MUFAs, with the exception of eicosenoic acid (C20:1n9). Pigs fed on 10% WDGS exhibited lower peroxidation of lipids (TBARS) than the control group or WDGS 15%. Similarly, water holding capacity (WHC) was the lowest for the group WDGS 10%. Of the meat coloration, redness (a*), yellowness (b*), and chroma (C*) were affected by the WDGS’ inclusion, where the highest values were observed for the group WDGS 10%. In conclusion, WDGS can be utilized in the liquid feeding of pigs for up to 15% of their DM.

Digestion characteristics and prediction of digestible energy and total digestible nutrients in beef cattle finishing diets containing traditional and by-product lipid sources

Our objective was to determine effects of supplemental fat sources on digestion and energy predictions in feedlot diets containing several lipid sources. Five ruminally fistulated steers (520 ± 41 kg of BW) were fed in a 5-period Latin square design. Dietary treatments included control (CORN) or 4 finishing diets containing (1) 4.8% added corn oil (OIL), (2) 4.8% beef tallow (TAL), (3) 25.5% corn condensed distillers solubles (CCDS), or (4) 56% wet distillers grains plus solubles (WDGS). Total-tract OM digestibility was multiplied by dietary OM content to determine digested OM (DIGOM; % of DM). Measured dietary DE (Mcal/kg) was converted to TDN using 4.4 Mcal of DE/kg of TDN. The unsaturated-to-saturated total fatty acid ratio of omasal samples of steers fed WDGS was greater than that for other treatments ( P = 0.01). Apparent total-tract diet NDF digestibility was least for steers fed OIL ( P < 0.04). The difference between TDN (% of DM) and DIGOM (% of DM) increased ( P < 0.01) as dietary GE increased across treatments. These results suggest DIGOM (% of DM) does not relate to TDN (% of DM) on a consistent basis when high-fat by-products are used and that measured DE (Mcal/kg) is needed to predict performance. In finishing diets containing WDGS, additional DE is not accounted for when evaluating only DIGOM. Measuring DE content of diets used in digestion trials is important when trying to estimate feeding values.

Effects of replacing wet distillers grains with supplemental SoyPass in forage-based growing cattle diets.

One hundred twenty individually fed steers (initial BW 283 kg ± 32) were utilized in an 84-d growing trial to evaluate the effects of increased metabolizable lysine from non-enzymatically browned soybean meal (SoyPass) in grass hay-based diets containing wet distillers grains plus solubles (WDGS). The treatments were arranged as a 2 × 3 factorial with two levels of protein supplement as 20% (low; DL20) or 35% (high; DL35) of dietary DM using WDGS as the basal protein source, and three increments of SoyPass (SP) replacing 0%, 30%, or 60% of the WDGS DM in the protein supplement yielding six dietary treatments. Average daily gain (ADG), dry matter intake (DMI), gain:feed (G:F), and plasma urea N (PUN) data were analyzed using the MIXED procedure of SAS as a 2 × 3 factorial. Animal was the experimental unit and fixed effects included body weight block, dietary inclusion of distillers grains (DL20, DL35), dietary inclusion of SoyPass (SP) (3 levels), and DL × SP inclusion interactions. Linear and quadratic interactions between DL and SP inclusion were analyzed using covariate regression. No interactions were detected for ADG between SP and DL (P = 0.76). Additionally, SP had no effect on ADG (P = 0.49). However, ADG was increased for steers consuming the DL35 diet compared to DL20 (1.13 vs. 0.86 kg/d, respectively; P < 0.01). A DL × SP interaction was detected for DMI (P = 0.01). As SP replaced WDGS in the DL35 diet, DMI increased linearly from 8.10 to 8.93 kg/d (P = 0.02). In the DL20 diet, DMI was not different as SP replaced WDGS (P ≥ 0.11). Therefore, G:F tended to decrease linearly (P = 0.06) as SP replaced WDGS in the DL35 diet, while no difference (P ≥ 0.11) was detected in the DL20 diet, suggesting SP contained less energy than WDGS but did improve dietary lysine balance. Furthermore, plasma urea nitrogen (PUN) increased linearly as SP replaced WDGS in the DL20 diet (P < 0.01) but was not affected by SP substitution in the DL35 diet (P ≥ 0.19). When WDGS is fed at a low (20% DM) or high (35% DM) inclusion rate in a forage-based diet, replacing the distillers with a source of protected amino acids supplied through heat-treated soybean meal, did not improve performance. A more concentrated or energy-dense form of amino acids may be beneficial in forage-based growing cattle diets containing 20% distillers grains but is not needed in diets with 35% distillers grains.

229 Evaluation of Wheat Blended with Corn in Finishing Diets Containing Wet Distillers Grains

Abstract An experiment was conducted to evaluate the effect of grain type and wet distillers grains inclusion on finishing cattle performance and carcass characteristics. Yearling steers (n = 320; initial BW = 325 ± 23 kg) were utilized in a 2 x 2 factorial arrangement, with the first factor as grain type at either 100% dry-rolled corn or a 50:50 blend of dry-rolled wheat and dry-rolled corn, and the second factor as wet distillers grains plus solubles (WDGS) inclusion at 12 or 30% of diet DM. There were no interactions between grain type and WDGS inclusion levels (P ≥ 0.21), contrary to our hypothesis that 50:50 wheat blend would improve performance with increased WDGS. There were no differences in DMI, ADG, or G:F (P ≥ 0.29) between 100% DRC or 50:50 blend of DRC and wheat. There was no difference in HCW between grain types (P = 0.84), but LM area was greater (P = 0.02) for steers fed a 50:50 blend of DRC and wheat. There were no differences in 12th rib fat or USDA marbling score between grain types (P ≥ 0.15), but increased LM area in steers fed the blended diet led to improved calculated yield grade (P = 0.04). Increasing WDGS in the diet increased carcass-adjusted final BW (P = 0.03) and improved carcass-adjusted ADG and G:F by 3.8% (P ≤ 0.05). Feeding 30% WDGS increased HCW by 10.9 kg (P = 0.03) and increased 12th rib fat (P = 0.02) compared to feeding 12% WDGS. There were no differences in LM area, USDA marbling score, or calculated YG between WDGS inclusions. These data suggest that increasing WDGS in the diet improves performance regardless of grain type and depending on price, wheat can replace up to 50% of the grain portion of the diet without affecting performance.

PSIX-1 Associative effect of wet-distillers’ grains plus solubles and tannin-rich peanut skin supplementation on in vitro rumen fermentation, greenhouse gas emissions, and microbiome changes

Abstract The role of tannin-rich peanut skin (PS) and associative effects of different levels of wet distillers’ grains plus solubles (WDGS) on ruminal fermentation, microbial changes, and mitigation of greenhouse gas (GHG) and other emissions in bovine rumen fluid were investigated. All gases were collected using an Ankom in vitro system for methane (CH4), nitrous oxide (N2O), and hydrogen sulfide (H2S) analyses. Fifteen % ground PS against 0, 10, 20, 30, and 40 % DM of WDGS were used. RT-qPCR were conducted to determine microbial diversity. In the absence of PS, total CH4 and H2S, or CH4 and H2S productions per gram of DM substrate, were linearly increased (P &amp;lt; 0.05) with increasing WDGS. However, in the presence of PS, those trends were reversed and CH4 and H2S productions were decreased (P &amp;lt; 0.05), suggesting that a diet with 15% PS and supplementation of 10 and 20% WDGS were able to reduce CH4 and H2S emissions by 12 and 33%, respectively. In the presence of PS, rumen fermentation rate (as a measured by VFA) and acetate/propionate (A/P) ratio was decreased with increasing WDGS, with PS x WDGS interactions (P &amp;lt; 0.01). In the presence of PS, there was a decreased (P &amp;lt; 0.05) the average population of Bacteroidetes, total methanogens, Methanobrevibacter sp. AbM4, and total protozoa populations at 40% WDGS, with PS x WDGS interactions (P &amp;lt; 0.01). The population of total methanogens (R2 = 0.57; P &amp;lt; 0.01), Firmicutes populations (R2= 0.46: P &amp;lt; 0.05), and F/B ratio (R2 = 0.46; P &amp;lt; 0.03) were strongly correlated with ruminal methane gas production. Therefore, associative effect of tannin-rich PS and WDGS suppressed methanogenesis pathways directly across their antimethanogenic activity and secondarily throughout their modification of protozoa population.

Associative effects of wet distiller's grains plus solubles and tannin-rich peanut skin supplementation on in vitro rumen fermentation, greenhouse gas emissions, and microbial changes1.

Two sets of in vitro rumen fermentation experiments were conducted to determine effects of diets that included wet distiller's grains plus solubles (WDGS) and tannin-rich peanut skin (PS) on the in vitro digestibility, greenhouse gas (GHG) and other gas emissions, fermentation rate, and microbial changes. The objectives were to assess associative effects of various levels of PS or WDGS on the in vitro digestibility, GHG and other gas emissions, fermentation rate, and microbial changes in the rumen. All gases were collected using an ANKOM Gas Production system for methane (CH4), carbon dioxide (CO2), nitrous oxide (N2O), and hydrogen sulfide (H2S) analyses. Cumulative ruminal gas production was determined using 250 mL ANKOM sampling bottles containing 50 mL of ruminal fluid (pH 5.8), 40 mL of artificial saliva (pH 6.8), and 6 g of mixed diets after a maximum of 24 h of incubation. Fermenters were flushed with CO2 gas and held at 39 °C in a shaking incubator for 24 h. Triplicate quantitative real-time polymerase chain reaction (qPCR) analyses were conducted to determine microbial diversity. When WDGS was supplied in the diet, in the absence of PS, cumulative CH4 production increased (P < 0.05) with 40% WDGS. In the presence of PS, production of CH4 was reduced but the reduction was less at 40% WDGS. In the presence of PS, ruminal lactate, succinate, and acetate/propionate (A/P) ratio tended to be less with a WDGS interaction (P < 0.01). In the presence of PS and with 40% WDGS, average populations of Bacteroidetes, total methanogens, Methanobrevibacter sp. AbM4, and total protozoa were less. The population of total methanogens (R2 = 0.57; P < 0.01), Firmicutes (R2 = 0.46: P < 0.05), and Firmicutes/Bacteroidetes (F/B) ratio (R2 = 0.46; P < 0.03) were strongly correlated with ruminal CH4 production. Therefore, there was an associative effect of tannin-rich PS and WDGS, which suppressed methanogenesis both directly and indirectly by modifying populations of ruminal methanogens.

336 Effects of supplemental SoyPass in forage-based diets containing distillers grains on performance of growing steers

Abstract One hundred twenty individually fed steers (initial BW 283 kg) were utilized in an 84-d growing trial to evaluate effects of increased metabolizable lysine from non-enzymatically browned soybean meal (SoyPass) in grass hay based diets containing wet distillers grains plus solubles (WDGS). Treatments were arranged as a 2 × 3 factorial design with two levels of WDGS (20% or 35% of DM) and three levels of supplemental SoyPass replacing 0%, 30%, or 60% of WDGS. The statistical model included animal as the experimental unit and level of WDGS and SoyPass substitution were included as fixed effects. Covariate regression was used to test for linear and quadratic interactions between WDGS and SoyPass substitution. No interactions were detected for ADG between SoyPass supplementation and level of WDGS in the diet (P = 0.76). Additionally, SoyPass inclusion had no effect on ADG (P = 0.49). However, ADG was increased for steers consuming the 35% WDGS diet compared to steers offered the 20% WDGS diet (1.13 vs. 0.86 kg/d, respectively; P &lt; 0.01). A SoyPass × WDGS interaction was detected for DMI (P = 0.01). As SoyPass replaced WDGS in the 35% diet, DMI increased linearly from 8.10 to 8.93 kg/d (P = 0.01). In the 20% WDGS diet, DMI decreased as SoyPass replaced 60% of the WDGS compared to 30% (7.68 vs. 8.36, P = 0.02). Therefore, G:F decreased linearly (P = 0.01) as SoyPass replaced WDGS in the 35% treatment and increased quadratically (P = 0.02) for the 20% WDGS treatment. Furthermore, BUN increased linearly as SoyPass replaced WDGS in the 20% diet (P = 0.01) but was not affected by SoyPass substitution in the 35% diet. Overall, results indicate forage-based growing diets formulated with low-levels of WDGS (&lt; 20%) may be deficient in metabolizable lysine, which could be supplied with SoyPass.

PSII-4 Effect of urea and distillers inclusion in dry rolled corn based diets on heifer performance and carcass characteristics

Abstract Crossbred heifers (n = 96) were utilized to study the effects of increasing wet distillers grains plus solubles (WDGS) and urea inclusion in a dry rolled corn (DRC) based finishing diet on performance and carcass characteristics. Heifers were individually fed using a calan gate system with a 2 × 2 factorial arrangement of treatments. Orts were collected once a week and analyzed for dry matter (DM) content and used to correct DM intake (DMI). Factors included WDGS inclusion at either 10 or 20% of diet DM and urea inclusion at either 0 or 1.2% of diet DM. Cattle were stratified by body weight (BW) and assigned randomly to treatment. Blood samples were taken at 3 points during the feeding period and analyzed for blood urea nitrogen content. The model included urea and WDGS inclusions and their interactions as fixed effects. There were no interactions (P &gt; 0.08) between WDGS and urea inclusion for any performance or carcass characteristics so only main effects are discussed. There was no difference for initial BW, final BW, ADG, and G:F on a live or carcass adjusted basis (P &gt; 0.26) for either urea or distillers inclusion in the diet. Dry matter intake was reduced (P = 0.03) with increased urea inclusion; however, WDGS inclusion did not influence DMI (P = 0.29). No difference (P &gt; 0.10) was detected for urea or WDGS inclusion for any carcass characteristics. Blood urea nitrogen was greater (P &lt; 0.01) for cattle fed increasing levels of both urea and WDGS. Added distillers and urea in the diet had minimal impact on performance or carcass characteristic suggesting supplemental urea in a DRC-based finishing diets is of minimal benefit when feeding at least 10% WDGS.

337 Effect of adding urea to finishing diets containing two different inclusions of distillers grains on steer performance and carcass characteristics

Abstract Crossbred beef steers (n = 384) were utilized to evaluate the effects of adding urea to a dry rolled corn based finishing diet containing low inclusions of wet distillers grains plus solubles (WDGS). Treatments were set up as a 2 × 4 factorial arrangement with factors consisting of WDGS inclusion at either 12 or 20% of diet dry matter (DM) and urea inclusion at either 0, 0.4, 0.8, 1.2% of diet DM. Cattle were blocked and stratified by initial body weight (BW) and assigned randomly to treatment. The heavy block of cattle were harvested after 160 d on feed (DOF) and the middle and light blocks of cattle were harvested after 179 DOF. The model include the fixed effects of weight block, WDGS inclusion, urea inclusion, and their interactions. There were no significant interactions (P &gt; 0.12) observed between WDGS inclusion and urea inclusion in the diet; therefore, only main effects are discussed. Increasing inclusion of WDGS improved (P &lt; 0.04) carcass adjusted ADG and G:F. Dry matter intake was reduced (P = 0.04) with increasing WDGS inclusions. Fat thickness was increased (P = 0.04) with increased WDGS inclusion; however, no other carcass characteristics were significantly affected by WDGS inclusion. Cubic effects of urea inclusion were observed (P &lt; 0.05) for HCW, final BW, ADG, and DMI on a carcass adjusted and live BW basis; however, cubic effects with increasing urea inclusion are of minimal biological relevance. There were no linear or quadratic responses (P &gt; 0.11) for increasing urea inclusion in the diet for performance measurements or carcass characteristics. Increasing WDGS inclusion in the diet improved performance. Added urea in the diet had minimal impact on performance, regardless of wet distillers grains plus solubles inclusion.

342 Evaluation of the effects of biochar on diet digestibility and methane production from growing and finishing steers

Abstract Two trials were conducted to evaluate the effects of biochar (0, 0.8, or 3% of diet dry matter) on diet digestibility and methane production in growing and finishing cattle diets. The growing diet consisted of 21% brome hay, 20% wheat straw, 30% corn silage, 22% wet distillers grains plus solubles (WDGS), and 7% supplement. The finishing diet consisted of 53% dry rolled corn, 15% corn silage, 25% WDGS, and 7% supplement. Biochar replaced fine ground corn in the supplement. Growing diets were evaluated over 6 periods in a switchback design, followed by the finishing trial with 3 periods in a crossover design using 6 steers (529 kg initial BW). Digestibility measures were taken over 4 d after at least 8 d of adaptation to diets followed by 2 d of gas emission measurements using headbox calorimeters. Statistical analysis included treatment and period as fixed effects and steer as a random effect with PROC IML of SAS used to generate coefficients for orthogonal contrasts. In the growing study, OM and NDF digestibility increased quadratically (P = 0.10) while OM digestibility tended to linearly decrease (P = 0.13) and NDF digestibility was not affected (P ≥ 0.39) by biochar inclusion in the finishing diet. Methane production (g/d) tended to decrease quadratically (P = 0.14) in the growing study and was decreased 10.7% for the 0.8% biochar treatment relative to the control. There were no statistical differences in methane production (g/d) in the finishing study (P ≥ 0.32). Methane production (g/kg DMI) from the 0.8% biochar treatment relative to the control was numerically reduced 9.5% and 18.4% in the growing and finishing studies, respectively (P ≥ 0.13). Although biochar is not an FDA-approved feed for cattle, initial research shows potential as a methane mitigation strategy in both growing and finishing diets.

Quality effects on beef strip steaks from cattle fed high-protein corn distillers grains and other ethanol by-products.

The objective of this study was to evaluate the effects of feeding high-protein corn distillers grains on fresh beef quality. Steers (n = 60) were fed one of five diets for 190 d (six pens with 10 cattle/pen/diet): a corn control (Control), 40% high-protein dry distillers grains plus solubles (HP-DDGS), 40% dry distillers grains plus solubles (DDGS), 40% wet distillers grains plus solubles (WDGS), or 40% bran plus solubles (Bran). Eighteen Choice carcasses (three cattle per pen) were randomly selected within each treatment; however, two carcasses were lost during carcass selection. Strip loins (Longissimus lumborum, IMPS # 180) were collected, divided into three equal portions, and aged for 2, 9, or 23 d. Steaks were fabricated following each aging period and placed under retail display (RD) conditions for 0, 4, or 7 d. Pen was the experimental unit. Hot carcass weight at harvest was 391 kg (SD 31.6 kg). Dietary treatment had no effect on tenderness (Warner-Bratzler shear force) within each aging period (P > 0.05). After 7 d of RD, following all aging periods, steaks from cattle fed HP-DDGS had the greatest visual discoloration except for DDGS and Bran after 23 d (P < 0.05). The steaks from cattle fed HP-DDGS had lower (P < 0.05) redness (a*) values than all other treatments following 7 d of RD. Lipid oxidation increased (P < 0.001) during RD at all aging periods, as measured via thiobarbituric acid reactive substance (TBARS). A day of RD-by-dietary treatment effect (P < 0.001) was observed for lipid oxidation. After 7 d of RD, steaks from cattle fed HP-DDGS had higher TBARS than all other treatments except WDGS (P < 0.01). A trend was found for sarcomere length (P = 0.07), with steaks from cattle fed Bran having the longest sarcomere length compared with all other treatments. An aging effect was found for free calcium content (P < 0.001) where steaks aged 9 and 23 d had significantly higher levels of free calcium than those aged 2 d. There was a tendency (P = 0.07) that steaks from cattle fed WDGS contained more free calcium than steaks from cattle fed DDGS or Bran. Although there were no statistical differences, there was a trend (P = 0.07) that steaks from cattle fed HP-DDGS had higher levels of PUFAs and C18:2. These results suggest that feeding high protein distillers grains has no detriment on tenderness, but may alter the lipid profile of the muscle, resulting in decreased color stability, increased lipid oxidation, and decreased shelf life.

Finishing performance and diet digestibility for feedlot steers fed corn distillers grains plus solubles and distillers solubles with and without oil extraction.

Three experiments evaluated the effects of corn oil removal using centrifugation in ethanol plants, on animal performance and digestion characteristics by finishing cattle fed by-products. In Exp. 1, 225 crossbred steers (300 ± 9.1 kg) were utilized in a randomized block design with a 2 × 2 + 1 factorial arrangement of treatments. Factors consisted of oil concentration [de-oiled (DO) or full fat (FF)] and by-product type [condensed distillers solubles (CDS) or modified distillers grains plus solubles (MDGS)] compared to a corn-based control. Fat concentration was 6.0% for DO CDS, 21.1% for FF CDS, 9.2% for DO MDGS, and 11.8% for FF MDGS. No oil concentration by by-product type interactions (P ≥ 0.17) were observed. There were no differences in DMI, ADG, or G:F between DO and FF CDS (P ≥ 0.29) or DO and FF MDGS (P ≥ 0.58). No differences (P ≥ 0.25) due to oil concentration were observed for carcass characteristics. Experiment 2 was a 5 × 5 Latin Square digestion trial with treatments similar to Exp. 1. Fat concentration was 8.7% or 15.4% for DO or FF CDS and 9.2% or 12.3% for DO or FF MDGS. Intake and total tract digestibility of fat were greater (P ≤ 0.02) for FF CDS compared with DO CDS. Digestible energy (megacalorie per kilogram), adjusted for intake, was greater (P = 0.02) for steers fed FF CDS compared to DO CDS. Average ruminal pH for cattle fed FF MDGS was greater than DO MDGS (P = 0.06). In Exp. 3, 336 yearling, crossbred steers (352 ± 19 kg) were utilized in a randomized block design with a 2 × 3 + 1 factorial arrangement of treatments. Factors included oil concentration (DO or FF) and inclusion [35%, 50%, and 65% wet distillers grains plus solubles (WDGS)] along with a corn-based control. The fat concentrations of DO and FF WDGS were 7.9% and 12.4%, respectively. A linear interaction (P < 0.01) was observed for DMI, which produced different slopes for DO and FF WDGS. No linear or quadratic interactions were observed for BW, ADG, or G:F (P ≥ 0.31). For the main effect of oil concentration, there were no statistical differences (P > 0.19) for final BW, ADG, or G:F. No statistical differences were observed for all carcass traits (P ≥ 0.34). Corn oil removal via centrifugation had minimal impact on finishing performance suggesting that cattle fed DO by-products will have similar performance to cattle fed FF by-products in dry-rolled and high-moisture corn diets.

Quality Effects of Beef from Cattle Fed High-Protein Corn Distillers Grains and Other Ethanol By-Products

ObjectivesThis research was conducted to evaluate the effects of feeding high protein corn distillers grains on beef tenderness during aging, sarcomere length, pH, proximate composition, and fatty acid compositionMaterials and MethodsSteers (n = 300) were fed for 190 d on either a corn control, 40% high protein dry distillers grains plus solubles (HP-DDGS), 40% dry distillers grains plus solubles (DDGS), 40% wet distillers grains plus solubles (WDGS), or 40% bran plus solubles (Bran) diet. Eighteen Choice carcasses (3 head/pen) were randomly selected within each treatment (n = 90). Strip loin samples from the right side were collected and aged for 2, 9, or 23 d. Steaks were fabricated at each aging period and placed under retail display conditions for 0 or 7 d. Tenderness (Warner-Bratzler shear force), sarcomere length, pH, proximate composition, and fatty acid composition were determined. Data were analyzed using the PROC GLIMMIX procedure of SAS (SAS Inst. Inc., Cary, NC) and pen was the experimental unit.ResultsHot carcass weight at harvest was 391 kg (SD 31.6 kg). Steaks aged 2 d showed no difference (P = 0.17) in tenderness across treatments, however a display effect (P < 0.0001) was present. Steaks that were under retail display conditions for 7 d exhibited a lower Warner-Bratzler shear force value than steaks not placed in retail display (3.40 kg and 4.27 kg, respectively). Steaks aged for 9 d and 23 d exhibited no differences across treatments (P = 0.14), however there was a significant age by treatment effect (P = 0.01). As HP-DDGS, DDGS, and Bran increased in aging an increase of tenderness was observed. There was no difference (P > 0.05) in tenderness between HP-DDGS and corn across aging periods. Retail display increased tenderness for steaks from 9 d and 23 d (P < 0.0001). No differences were observed among treatments for sarcomere length (P = 0.07), fat (P = 0.51), moisture (P = 0.71), or ash (P = 0.74). An aging effect was found for pH (P < 0.0001), however, the values were of little practical significance (5.49 23 d, 5.48 2 d, 5.44 9 d, respectively). Preliminary results suggest beef from cattle fed HP-DDGS had significantly higher (P = 0.03) levels of 18:2 in muscle than cattle fed corn (480.61 mg/100g vs. 272.49 mg/100g). All other treatments presented intermediate levels of 18:2 (395.92 mg/100g for DDGS, 350.06 mg/100g for WDGS, and 321.53 mg/100g for Bran). Cattle fed corn had significantly more (P = 0.03) 17:1 in their muscle when compared to Bran (85.51 mg/100g vs. 57.76 mg/100g).ConclusionThese results suggest that feeding high protein distillers grains have no detriment on tenderness, but may alter the lipid profile of the muscle.

Effects of finishing diets containing wet distillers grains plus solubles on beef quality attributes and fatty acid profile

The objective of this study was to evaluate the effects of feeding wet distillers grains plus solubles (WDGS) on quality attributes of three beef muscles (longissimus lumborum, psoas major, and infraspinatus). Ninety-six, yearlings crossbred steers were randomly assigned to one of three dietary treatments (Corn, 15%, or 30% WDGS - DM basis) and fed for 133 d. No significant differences were observed in marbling score (P=0.89), marbling texture (P=0.70), and marbling distribution (P=0.36). Greater concentrations of PUFA and lower levels of 18:1(n-7) were observed in beef from steers fed 30% WDGS when compared to other treatments. Lipid oxidation was also greater in beef from steers fed 30% WDGS (P≤0.05). No significant differences were observed in sensorial attributes and Warner-Bratzler shear force (WBSF) for all muscles (P>0.05). Feeding WDGS increased PUFA and lipid oxidation, which may lead to shorter shelf life.

Feeding wet distillers grains plus solubles contributes to sarcoplasmic reticulum membrane instability

Feeding wet distillers grains plus solubles (WDGS) increases polyunsaturated fatty acid (PUFA) levels in beef. It was hypothesised that WDGS in feedlot diets increases PUFA concentration in the sarcoplasmic reticulum (SR) membrane, thereby altering membrane integrity, resulting in more rapid intracellular calcium leakage and improved tenderness. The objective of this study was to evaluate this hypothesis. Ninety-six crossbred steers were fed either a corn-based diet with 0% WDGS or 50% WDGS. Fifteen strip loins per treatment were collected, fabricated into steaks, aged and placed under retail display conditions. Steaks were used to measure tenderness, proteolysis, free calcium concentrations, lipid oxidation, sarcomere length and SR membrane fatty acid, phospholipid lipid, neutral lipid and total lipid profiles. Compared with steaks from steers fed 0% WDGS, steaks from steers fed 50% WDGS were more tender (P &lt; 0.05) and had greater (P &lt; 0.05) free calcium concentrations early post-mortem. Feeding 50% WDGS also tended to increase (P &lt; 0.10) total PUFA concentrations, decrease (P &lt; 0.10) total phospholipid concentration and increase (P &lt; 0.10) total neutral lipid concentration for SR membrane. Steaks from steers fed 0% WDGS had greater (P &lt; 0.05) lipid oxidation (TBARS values) than steaks from steers fed 50% WDGS after extended aging. Although differences in tenderness between the two treatments were detected, there were no corresponding differences (P &gt; 0.10) in sarcomere length or proteolysis. This study showed that feeding WDGS may increase tenderness, possibly by increasing free calcium in muscle early post-mortem. However, the true mechanism that contributes to these differences is still unclear.

Effects of dietary antioxidant supplementation of steers finished with 30% wet distillers grains plus solubles on fatty acid profiles and display life of strip loins

Feeding wet distillers grains plus solubles (WDGS) in beef feedlot diets increases beef polyunsaturated fatty acids (PUFA) concentration and decreases shelf-life; whereas feeding antioxidants like vitamin E and Agrado-Plus (AG; an ethoxyquin and tert-Butylhydroquinone mixture) mitigates such effects. The objective of this study was to evaluate shelf-life of beef from steers supplemented with WDGS and different antioxidants. One hundred and sixty steers were finished on a corn-based diet with 0% WDGS or 30% WDGS and four antioxidant treatments (no supplementation; vitamin E only; AG only; vitamin E + AG). Ten strip loins from each treatment were collected, aged, cut into steaks, and placed under retail display (2 ± 2°C). During retail display, steaks were evaluated daily for objective colour and subjective discolouration. After retail display, steaks were used to measure lipid oxidation, muscle fatty acids, and vitamin E and ethoxyquin concentrations. Feeding 30% WDGS increased total PUFA in beef when compared with beef from steers fed 0% WDGS (P &lt; 0.05). Supplementing vitamin E or vitamin E + AG was effective in decreasing myoglobin and lipid oxidation in steaks from steers fed 0% or 30% WDGS after retail display (P &lt; 0.01). Supplementation of vitamin E or AG also increased (P &lt; 0.01) muscle tissue vitamin E or ethoxyquin levels, respectively, compared with the diets without vitamin E or AG supplementation. The inclusion of 30% WDGS altered beef fatty acid profiles, but did not promote lipid and myoglobin oxidation compared with the 0% WDGS diet. Feeding vitamin E was effective, whereas supplementing AG had minor effects in decreasing myoglobin and lipid oxidation in steaks from both diet.

Impact of feeding de-oiled wet distillers grains plus solubles on beef shelf life.

Research was conducted to determine the effect of feeding de-oiled wet distillers grains plus solubles (WDGS) on beef fatty acid profile, retail shelf life and development of oxidation products during retail display (RD). A total of 336 crossbred yearling steers (initial BW = 351.08 ± 19.05 kg) were fed 1 of 7 dietary treatments: an all corn control (1:1 blend of dry rolled and high moisture corn), 35%, 50%, or 65% inclusion of WDGS, either full-fat or de-oiled. Within each treatment 15 Choice carcasses were randomly selected ( = 105), strip loins were obtained, aged 7 and 21 d, and representative steaks from each strip loin were placed in RD conditions for 7 d. Fatty acid profiles were determined (mg/100 g tissue basis) and differences ( ≤ 0.05) were found in the C16:1, C18:1T, C18:2 and total polyunsaturated fatty acids (PUFA) among dietary treatments. Palmitoleic acid (C16:1) was predominant ( < 0.0001) in the corn control group, intermediate in the 35% de-oiled WDGS group, but no differences ( > 0.05) were observed between all other diets. Elaidic acid (C18:1T) was greater ( = 0.01) in the 65% full-fat WDGS group, least for the corn control group, and intermediate for all other diets. Linoleic acid (C18:2) was greater ( = 0.0001) in all 3 full-fat WDGS groups and 65% de-oiled WDGS group (290.98 mg/100 g, on average), intermediate in the 50% and 35% de-oiled WDGS groups (231.08 and 227.16 mg/100 g, respectively) and least for the corn control group (177.70 mg/100 g). The PUFA content was greater ( < 0.01) in all 3 full-fat WDGS groups and 65% de-oiled WDGS group (337.13 mg/100 g, on average), intermediate in the 50% and 35% de-oiled WGDS groups (274.77 and 273.84 mg/100 g, respectively) and least for the corn control group (223.98 mg/100 g). Dietary treatment did not alter discoloration ( = 0.30) or lipid oxidation ( = 0.36). Shear force decreased with age and RD ( < 0.0001) but dietary treatment had no effect on shear force ( = 0.93). In general, feeding 35% and 50% de-oiled WDGS had intermediate PUFA content relative to a corn control or full-fat WDGS diet. Feeding de-oiled WDGS did not seem to increase beef shelf life and does not negatively alter beef quality parameters in relation to full-fat WDGS.

Feeding vitamin E may reverse sarcoplasmic reticulum membrane instability caused by feeding wet distillers grains plus solubles to cattle

ABSTRACT Research has shown feeding wet distillers grains plus solubles (WDGS) in beef feedlot diets increases PUFA concentration in the sarcoplasmic reticulum (SR) membrane, thereby altering membrane integrity, resulting in more rapid postmortem calcium leakage and improving tenderness through early activation of calcium-dependent proteases. Supplementation with antioxidants may mitigate such effects. A total of 160 steers were finished on a corn-based diet with 0 or 30% WDGS and 4 antioxidant treatments [no supplementation; vitamin E; Agrado; vitamin E plus Agrado]. Ten strip loins from each treatment were collected, aged, cut into steaks, and placed under retail display. Tenderness, free calcium, sarcomere lengths, proteolysis, and SR membrane phospholipid and fatty acid profiles were measured. The 30% WDGS diet increased ( P P = 0.05) phosphatidylcholine in the SR membrane but less ( P P P > 0.10). These results suggest that the alteration of SR membrane fatty acid profile in WDGS-fed cattle may affect the ratio of SR membrane phospholipids, creating membrane instability, and thereby increasing proteolysis, whereas feeding vitamin E mitigates such effects.

Feeding wet distillers grains plus solubles with and without a direct-fed microbial to determine performance, carcass characteristics, and fecal shedding of O157:H7 in feedlot heifers.

The inclusion of wet distillers grains plus solubles (WDGS) in feedlot diets has become a common practice in many regions of the United States due to the expanded production of byproducts and fluctuating corn prices related to ethanol production and other factors. In addition, societal concerns over the continued use of antimicrobials in agriculture production combined with an enhanced interest in disease and pathogen prevention in the food supply have led to an increased interest in use of direct-fed microbials (DFM) in growing and finishing cattle. Direct-fed microbials have been shown to improve ADG and feed efficiency, alter ruminal fermentation, and decrease fecal shedding of potential harmful pathogens in feedlot cattle in some experiments. The objective of this experiment was to evaluate the effects of WDGS inclusion with or without a DFM containing (1 × 10 cfu ∙ heifer ∙ d) combined with (1 × 10 cfu ∙ heifer ∙ d) on the performance, carcass characteristics, and O157:H7 shedding in feedlot heifers. In early August, 288 crossbred heifers (initial BW = 295 ± 28 kg) were assigned to 1 of 4 treatments (12 pens per treatment; 6 heifers per pen) in a randomized complete block design with a 2 × 2 factorial arrangement of treatments. Body weights and fecal grab samples were obtained at approximately 28-d intervals throughout the experiment. Across the feeding period, heifers fed 30% WDGS tended ( = 0.09) to have greater ADG and had greater carcass-adjusted ADG ( = 0.05) compared with heifers fed dry-rolled corn (DRC). Dry matter intake was not affected ( = 0.65) by diet, although carcass-adjusted G:F tended ( = 0.10) to be improved for heifers fed WDGS. Heifers fed 30% WDGS tended ( ≤ 0.10) to have greater fat thickness at the 12th rib, lower marbling scores, and higher yield grades. The inclusion of . combined with . in the diet had no effect ( > 0.10) on performance or carcass merit in the present experiment. The incidence of O157:H7 throughout the experiment was low, with only 18 positive samples across all sampling periods. Neither WDGS inclusion nor the inclusion of . combined with . in the diet had any effect ( > 0.10) on O157:H7 shedding in this experiment. Feeding 30% WDGS to feedlot heifers improved animal performance compared to the DRC-based control diet.

Effect of corn particle size with moderate amounts of wet distillers grains in finishing diets on starch digestibility and steer performance

Five hundred yearling steers (370 ± 30.0 kg, SD) were used to determine the effect of corn particle size in diets containing 35% wet distillers grains plus solubles (WDGS) on steer performance, carcass characteristics, and apparent total-tract starch digestibility (TTSD). Treatments included 45% cracked corn (CON; 2,350 μm) or finely ground corn (FIN; 500 μm) with 35% WDGS and were replicated in 4 pens per treatment with 60 or 64 steers per pen. Fecal samples were collected on d 71 and 72 (F-1) and d 102 and 103 (F-2). Final BW and HCW were heavier (P ≤ 0.01) for steers finished on CON compared with steers finished on FIN. Whereas G:F was not different (P = 0.22) between treatments, DMI and ADG were greater (P ≤ 0.01) for CON-fed steers than FIN-fed steers. Liver abscess scores were not influenced (P ≥ 0.39) by treatment. Liver abscess scores tended (P = 0.10) to influence ADG over the experiment, with steers having severe liver abscess scores gaining less compared with steers with no or mild liver abscess scores. A treatment × time effect (P < 0.01) was observed for TTSD. Whereas TTSD of steers fed CON decreased over time (90.28 and 85.74% for F-1 and F-2, respectively), TTSD of steers finished on FIN did not differ across the 2 sampling dates (97.95 and 97.55% for F-1 and F-2, respectively). Apparent starch digestibility was improved for steers fed finely ground corn with 35% WDGS; however, cattle performance was less compared with steers fed cracked corn with 35% WDGS.