Event Abstract Back to Event NGS corroborates cryptic diversity within the Epiros riffle dace (Telestes pleurobipunctatus) Nuria VIÑUELA RODRÍGUEZ1*, Radek ŠANDA2, Lubomír PIÁLEK3, Stamatis Zogaris4 and Jasna VUKIĆ1 1 Department of Ecology, Division of Biology, Faculty of Science, Charles University, Czechia 2 National Museum (Czechia), Czechia 3 Faculty of Science, University of South Bohemia, Czechia 4 Hellenic Centre for Marine Research (HCMR), Greece The geological history of the Southern Balkans is genuinely complex and it has deeply influenced aquatic species evolution. The Ionian inland waters host the largest proportion of the Greek endemic ichthyofauna. Recent works have shown that the diversity of freshwater fishes of this region has been underestimated. The Eastern Adriatic coast is considered as a “hot spot” for genus Telestes, with small regions displaying exceptionally high diversity. In contrast, in the Ionian basin, a single species of Telestes is currently recognized. The Epiros riffle dace (Telestes pleurobipunctatus, Leuciscidae, Cypriniformes) is an Ionian endemic distributed from southern Albania (Butrint lagoon catchment) to Western Peloponnese (Alfios River basin). Previous study revealed the existence of five different lineages within T. pleurobipunctatus by the use of the mitochondrial marker cytochrome b. The occurrence of two different lineages within Louros river basin (south of Epiros region, flowing into Amvrakikos Bay) has also been reported. The aim of this study is to conduct a comprehensive study of T. pleurobipunctatus complex with special emphasis on the Louros River populations. We combined traditional sequencing of cytochrome b with the NGS method of ddRAD (double digest restriction-site associated DNA) sequencing. A dataset of 99 individuals from 11 river basins were analyzed with ddRAD-seq covering the known distribution range of the species. Additionally, over 150 individuals were analyzed by classical sequencing, 91 of them corresponding to populations within Louros catchment. Genome wide data have confirmed the existence of the previously revealed five well-supported lineages: Alfios lineage occurring in the Peloponnese, Evinos-Kotichy-Pinios lineage occurring in the north-west of the Peloponnese Peninsula and south of the mainland, and Acheloos lineage, Arachthos lineage and Northwestern lineage (Acheron-Kalamas-Kerkyra-Butrint) occurring in the northwest of the Ionian basin. Occurrence of two lineages was also confirmed in the Louros river basin. In the upper part of Louros River, the Acheron-Kalamas-Kerkyra-Butrint lineage is found, while in the lower course Arachthos lineage is present. This result can be explained as a case of a secondary contact of unknown origin. In conclusion, our results confirm that the taxonomic revision of this species complex is urgently needed. Our results should be taken into account for the conservation management of T. pleurobipunctatus species complex. Keywords: ddRAD sequencing, Telestes pleurobipunctatus, Cryptic diversity, phylogeny, Phylogeography Conference: XVI European Congress of Ichthyology, Lausanne, Switzerland, 2 Sep - 6 Sep, 2019. Presentation Type: Oral Topic: EVOLUTIONARY GENOMICS OF FISHES Citation: VIÑUELA RODRÍGUEZ N, ŠANDA R, PIÁLEK L, Zogaris S and VUKIĆ J (2019). NGS corroborates cryptic diversity within the Epiros riffle dace (Telestes pleurobipunctatus). Front. Mar. Sci. Conference Abstract: XVI European Congress of Ichthyology. doi: 10.3389/conf.fmars.2019.07.00038 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 20 Jul 2019; Published Online: 14 Aug 2019. * Correspondence: Mx. Nuria VIÑUELA RODRÍGUEZ, Department of Ecology, Division of Biology, Faculty of Science, Charles University, Prague, Prague, 12844, Czechia, nuria.vinuela.rdgz@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Nuria VIÑUELA RODRÍGUEZ Radek ŠANDA Lubomír PIÁLEK Stamatis Zogaris Jasna VUKIĆ Google Nuria VIÑUELA RODRÍGUEZ Radek ŠANDA Lubomír PIÁLEK Stamatis Zogaris Jasna VUKIĆ Google Scholar Nuria VIÑUELA RODRÍGUEZ Radek ŠANDA Lubomír PIÁLEK Stamatis Zogaris Jasna VUKIĆ PubMed Nuria VIÑUELA RODRÍGUEZ Radek ŠANDA Lubomír PIÁLEK Stamatis Zogaris Jasna VUKIĆ Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.
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