Western Ireland Research Articles

Genetic and morphological evidence support the specific status of the endemic Ericaandevalensis (Ericales, Ericaceae).

Assessing the taxonomic status of closely related taxa is crucial in plant systematics and can have important implications for conservation and human plant use. Ericaandevalensis Cabezudo & Rivera is a metallophyte endemic species from highly metal-polluted soils of SW Iberian Peninsula, an area with a mining history going back more than 5,000 years. Ericaandevalensis is closely related to Ericamackayana Bab., a northern Iberian species also present in western Ireland. The status of E.andevalensis as a species or subspecies subordinated to E.mackayana is subject to debate. Here, we assessed the genetic and phenotypic relationship between both species, including the population structure of E.andevalensis. We used high throughput sequencing to determine genome-wide Single Nucleotide Polymorphisms (SNPs), and morphometric analyses from 35 reproductive and vegetative traits. The morphological analysis showed at least eight characters that can discriminate the two species, from which ovary hairiness and the size of leaf glandular hairs were the most informative. Genetic analyses showed that each species formed a monophyletic cluster with full support, separated by an interspecific genetic distance >4-fold higher than intra-specific distance. Population genetic analyses of E.andevalensis shows that populations are highly structured, with the Portuguese one as the most isolated and less variable. These results support the recognition of E.andevalensis as a distinct species with a highly constrained ecological requirements and a narrow geographic distribution, but with a limited gene flow between populations. We discuss the implications of these outcomes in conservation policies and potential uses of E.andevalensis such as decontamination of polluted soils.

Holocene vegetation and flora dynamics of the west Cork/Kerry region, south-western Ireland

AbstractSediments from a small lake, Loch Beag, in Barrees, Beara peninsula, south-western Ireland, provide a record of vegetation history that spans most of the Lateglacial and Holocene (14–0.43 ka). The detailed part of the record relates to the Holocene, which is the focus of this paper. The investigations carried out include pollen and macrofossil analyses, loss-on-ignition measurements and 14C dating. As well as providing records for tree and shrub taxa, and especially Pinus, Quercus, Ulmus, Fraxinus and Taxus, other taxa mainly with Atlantic and/or Lusitanian distributions are also considered. These include Arbutus unedo (strawberry tree) and fern species such as Osmunda regalis, and the filmy ferns Hymenophyllum tunbrigense, H. wilsonii and Trichomanes speciosum (Killarney fern). Detailed records for a suite of bog/heath taxa, including ericoid species (Erica tetralix and E. cinerea), Narthecium ossifragum, Hypericum elodes and Myrica gale, are presented. The results of 14C dating of bog-pines from western Ireland are summarised. Results of other investigations, including key Holocene pollen diagrams, and charcoal and fossil-wood records from archaeological excavations that relate mainly to the Bronze Age in the wider west Cork/Kerry region, are reviewed. Vegetation dynamics, human impact and plant biogeography are discussed in the light of the considerable data now available for the region.

CORRECTION: Temporal variation in sea trout Salmo trutta life history traits in the Erriff River, western Ireland

CORRECTION: Temporal variation in sea trout Salmo trutta life history traits in the Erriff River, western Ireland

An Assessment of the Role of the Timex Sampling Strategy on the Precision of Shoreline Detection Analysis

Remote video imagery using shoreline edge detection is widely used in coastal monitoring in order to acquire measurements of nearshore and swash features. Some of these systems are constrained by their long setup time, positioning requirements and considerable hardware costs. As such, there is a need for an autonomous low-cost system (~EUR 500), such as Timex cameras, that can be rapidly deployed in the field, while still producing the outcomes required for coastal monitoring. This research presents an assessment of the effect of the sampling strategy (time-lapse intervals) on the precision of shoreline detection for two low-cost cameras located in a remote coastal area in western Ireland, overlooking a dissipative beach–dune system. The analysis shows that RMSD in the detected shoreline is similar to other studies for sampling intervals ranging between 1 s and 30 s (i.e., RMSDmean for Camera 1 = 1.4 m and Camera 2 = 0.9 m), and an increase in the sampling interval from 1 s to 30 s had no significant adverse effect on the precision of shoreline detection. The research shows that depending on the intended use of the detected shorelines, the current standard of 1 s image sampling interval when using Timex cameras can be increased up to 30 s without any significant loss of accuracy. This positively impacts battery life and memory storage, making the systems more autonomous; for example, the battery life increased from ~10 days to ~100 days when the sampling interval was increased from 1 to 5 s.

Antioxidant micropeptins from a Microcoleus autumnalis-dominated benthic cyanobacterial mat from Western Ireland

Exploring the chemical diversity present in cyanobacterial mats increasingly frequent in fresh and marine waters is imperative for both evaluating risks associated with these diverse biofilms and their potential for biodiscovery. During a project aimed at the study of the (eco)toxicity of benthic cyanobacteria blooming in some lakes of the West of Ireland, three previously undescribed ahp-cyclodepsipeptides micropeptin LOF941 (1), micropeptin LOF925 (2) and micropeptin LOF953 (3) were isolated from the Microcoleus autumnalis-dominated benthic cyanobacterial biofilm collected from the shore of Lough O'Flynn, Co. Roscommon, Ireland. Their structures remain consistent in their amino acid sequence with the presence of an unusual methionine, and differ by their exocyclic side chains. The planar structures of the previously undescribed micropeptins were elucidated by 1D and 2D NMR and HRESIMS analyses, and their 3D configurations assessed by ROESY NMR and Marfey's analyses. The three isolated compounds showed no cytotoxic effects and all three compounds were shown to exhibit antioxidant properties, with 1 showing the highest bioactivity. Additionally, several micropeptin analogues are proposed from the methanolic fraction of the biofilm extract by UHPLC-HRESIMS/MS analysis and molecular networking. Notably, the known cyanotoxins anatoxin-a and dihydroanatoxin-a were annotated in the molecular network therefore raising issues about the toxicity of this cyanobacterial mat.

Discovery of British and Irish bryophytes 1. Trends in the detection and reporting of new species, 1538–2021

ABSTRACT Introduction We examine the discovery of British and Irish bryophytes, based on a database of their first records. For 1140 taxa in the current (2021) checklist we have compiled information on the place and date of the first record, the finder and the author and place of its publication. The full database is posted on the Zenodo repository. Here we analyse changes over time in the attributes (e.g. size) of newly discovered species, their location and the main finders. Methods We checked first records reported in bryological publications, extracting the relevant details for our database. Most are supported by herbarium specimens. To check on these we have relied mainly on the expertise of others, examining only a few specimens ourselves. Key results Unlike flowering plants, very few bryophytes were known until ca. 1690 and the first to be discovered tended to be widespread, large species with frequent sporophytes, growing in lowland England. After Dillenius’s Historia muscorum (1741) there was a major trough in activity, extending to the 1780s. Thereafter there was a surge in records from the Highlands of Scotland (from 1790) and western Ireland (from 1809). The flood of new moss records in the early nineteenth century culminated in the 1850s, with William Wilson making an outstanding contribution. New liverwort records increased later in the century and peaked between 1900 and 1909. There followed a further trough for both groups from 1920 to 1949. Since then the rate of discovery has remained steady at about 2.8 species per year.

Migratory history of the threespine stickleback Gasterosteus aculeatus in western Ireland

Microchemical analysis of trace elements in otoliths and bio-mineralised earstones of teleost fishes is an emerging approach to analysing the environmental migratoryand life histories of fish species. The migration history of the three-spine stickleback (Gasterosteus aculeatus) collected in western Ireland was examined using calcium (Ca) and strontium (Sr) concentrations in otoliths. The otolith Sr:Ca values fluctuated with the habitat. The habitat use and migration history of G. aculeatus can be categorised into two types, as determined by the mean value and life history transect of the otolith Sr:Ca; that is, freshwater and estuarine residents, whereas there were no anadromous sticklebacks which is believed to be a typical migration pattern in the species. The otolith Sr:Ca profiles of the freshwater resident fishes exhibited constantly low Sr:Ca values, averaging 0.41–0.58 × 10−3 from the core towards the edge. However, the otolith Sr:Ca profiles of the estuarine resident fishes exhibited constantly high Sr:Ca values from the core towards the edge without a clear transition point from low to high Sr:Ca values, as found in the anadromous fish, averaging 1.82–4.26 × 10−3. The present study is the first published confirmation that 100 % of sticklebacks living in coastal habitats in Ireland > have an estuarine resident migratory pattern, constantly residing in marine environments or brackish water throughout their lifespan and not in freshwater environments in Ireland.

Phytoplankton abundance and biodiversity in adjacent estuaries: the importance of submarine groundwater discharge

A near-unique pair of adjacent bays in the karst region of western Ireland provided the opportunity to compare phytoplankton diversity and abundance in an area of submarine groundwater discharge (SGD) side by side with a ‘control’ region of similar size and with the same marine influences. Freshwater input to Kinvara Bay comes from the surrounding karst hinterland via a subterranean groundwater network, emerging at several sites within the inner bay as submarine groundwater discharge (SGD). Aughinish Bay in contrast has no connection to a SGD network and negligible overground fresh water input. Sampling was carried out in both bays during spring and neap tides over Winter, Spring/Summer and Summer/Autumn of 2016 and 2017. Comparison of the phytoplankton communities in the two bays showed higher diversity in Aughinish, while a bloom of Prorocentrum micans (Ehrenber, 1834), a brackish-water-tolerant marine dinoflagellate, was observed only in Kinvara. This work demonstrates how SGD can control phytoplankton community structure even in well-flushed macro-tidal bays and thus controls the biogeography at local scales.

Historical trends of floating wind turbine fatigue loads (Ireland 1920–2010)

We present a new method for analysing the fatigue loads of offshore floating wind turbines over the long term. In this method, bias-corrected wind and wave data from the 20th century (ERA20C versus ERA5 reanalysis) is used for an energetic sea location in western Ireland. To reduce the computational cost and theoretical complexity, the sea states were clustered into categories to indicate how these categories evolve over three climate periods during a period of 30 years (1921–1950, 1951–1980, and 1981–2010). OpenFAST aeroelastic code simulation in floating mode was then conducted at each sea state for spar-type 5-MW and semi-submersible 15-MW wind turbines. This shows the damage loads and fatigue increments over the century, with some relevant cases (rotor torque, blade pitch and flapping, and tower side–side and fore–aft moments) showing fatigue increments of 5%–8%. Thus, in the new method, historical data are used to create a model to represent the evolution of sea states and corresponding fatigue over the long term, which can be applied globally to future projections.

Molecular characterisation of Pinus sylvestris (L.) in Ireland at the western limit of the species distribution

BackgroundScots pine (Pinus sylvestris L.) underwent significant population declines across much of northwest Europe during the mid-to-late Holocene and was thought to have become extirpated in Ireland from about 400 AD. However, most extant populations are plantations reintroduced from Scotland. Others are naturalised therefrom and one in Western Ireland is a putative relict. In this paper, Scots pine in Ireland are genetically described for the first time.ResultsUsing two mitochondrial (mtDNA) loci, eight chloroplast (cpSSR) and 18 nuclear (nSSR) loci, the genetic composition and diversity of 19 Irish Scots pine populations is described and compared to other European populations. All trees sampled in Ireland were fixed for mitotype a, which is the most common across northwest Europe. By contrast, cpSSR (HCP = 0.967) and nSSR (He = 0.540) variation was high, and comparable with estimates for other regions across the species range. Differentiation at both sets of loci were similarly low (cpSSR FST = 0.019; nSSR FST = 0.018), but populations from continental Europe were significantly differentiated from all Irish populations based on nSSR variation.ConclusionsAll Irish Scots pine are likely part of a common Irish-Scottish gene pool which diverged from continental Scots pine following post-glacial recolonisation. A high genetic diversity and an absence of evidence of inbreeding suggests the regional decline of Scots pine did not critically reduce allelic variation. The post-glacial relationship between Irish and Scottish pine is discussed, and a suggestion from recent palaeoecological work that reintroduced Scots pine be managed as a native species is now further supported by genetic data.

Click-click, who’s there? Acoustically derived estimates of sperm whale size distribution off western Ireland

Understanding the structure of populations is a critical element to the establishment of management and conservation measures. Sperm whales Physeter macrocephalus are characterised by a demographic spatial segregation, associated with a conspicuous sexual dimorphism reflected in their vocalisations. These characteristics make acoustic techniques very relevant to the study of sperm whale population structure, especially in remote, challenging environments. The reliability of using inter-pulse intervals of sperm whale clicks to infer body size has long been verified and extensively used. We provide the first size structure estimates of the sperm whale population in an area where assumptions on population structure mainly relied on sparse observations at sea, whaling records and stranding data. Over 10,000 hours of acoustic data collected using both static acoustic recorders and towed hydrophone arrays in Irish offshore waters were processed using a machine learning-based tool aimed at automatically extracting inter-pulse intervals from sperm whale recordings. Our analyses suggested that, unlike previously thought, large males would not account for the majority of the animals recorded in the area. We showed that adult females/juvenile males (length 9-12 m) were predominant, accounting for 49% (n = 788) of the number of individuals recorded (n = 1,595), while the proportions of immature individuals (length<9 m) and adult males (length >12 m) were well balanced, accounting for 25% (n = 394) and 26% (n = 413) of the recorded whales, respectively. Our data also suggested some size segregation may be occurring within the area, with smaller individuals to the south. The implications of such findings are crucial to the management of the population and provide an important baseline to monitor changes in population structure, particularly relevant under changing habitat conditions.

The River Suck-Hind tochailt—One Event in the Very Active Kingship of Toirrdelbach Ó Conchobair, King of Connacht and High-King of Ireland, 1119–1156

THE CAREER OF TOIRRDELBACH Ó CONCHOBAIR, King of Connacht and High-King of Ireland, served as a high-water mark in terms of the authority and aspirations visible among pre-Norman Gaelic rulers. We will investigate one episode in his career, the AD 1139 commissioning of a major action of communal labour in western Ireland. This will confirm the course of this earthwork, understand its functions, and use this endeavour as a case study in examining the ambitions of this remarkable king, and the influences which shaped his decisions. This 12th - century tochailt (Ir digging) is made up of physical elements, some natural and some manmade. The natural course of the Suck flows southwards from west Roscommon towards Ballinasloe to join the Shannon at Raghrabeg, south-west of Shannonbridge. The Hind flows eastwards from south of Rocksavage House to Lough Ree on the Shannon. Between the headwaters of the Hind at Rocksavage and the Suck at Castlestrange Bridge, north of Athleague, a distance of 13.5 km, are the Ballinturly and Correal turloughs. It is the three lengths of works linking the turloughs to each other and to the two rivers that constitute the tochailt. This use of water formed a barrier between modern south Roscommon and mid-Roscommon, protecting and regulating access into and out of the traditional territory of the Gaelic Uí Chonchobair kings of Connacht to the north of this boundary throughout the later medieval period and beyond.

New data on the Ben Levy anticline, with reverse reactivation on the Coolin Fault giving up-side-down Silurian rocks in Joyces Country, Connemara

Abstract: In Joyces Country in western Ireland, the ∼ E-W striking axial plane of the D7 Ben Levy anticline folded Dalradian and unconformable Silurian rocks. The north side of the fold is mostly coincident with the contemporaneous ESE-WNW striking steeply dipping Coolin Fault close to the Dalradian-basal Silurian contact. This fault was first initiated by sinistral strike-slip motion, probably of Ordovician age, and then secondly used by post-Wenlock down throw on the north side to form the D7 anticline. Later a newly recognised third motion of reverse reactivation on the fault uplifted the north limb of the fold and locally overturned the Silurian succession against irregularities in the SSW dip of the fault. Still later, but pre-374Ma (Devonian), NE-SW faulting exposed different previous levels of the Coolin Fault zone. The superb Dalradian rock exposure provides details not generally apparent on other reactivated faults in Connemara.

Structural control of the deposition and subsequent deformation of the Killary Habour-Joyce Country Silurian succession

Abstract: The Killary Harbour–Joyce Country succession of Silurian rocks forms the remnant of one of three successor basins that developed after the Grampian Orogeny and which are preserved in western Ireland. Its outcrop everywhere obscures the contact between the Dalradian rocks of Connemara and the Ordovician rocks of the South Mayo Trough. The outcrop is bisected by the prominent north-west trending Maam Valley Fault Zone that is shown to have a component of synsedimentary movement down-throwing to the southwest. The original basin margins lay well outside the current outcrop area. North-south shortening and inversion of the basin was accomplished by reactivation and inversion of earlier fault structures and was exclusively in the brittle zone. Folding of the Silurian rocks with weak to moderate cleavage and deformation of the basement rocks of Connemara was accomplished by largely vertical movements along numerous strike parallel and oblique fractures. Break-back inversion structures were developed along the southern margin of the South Mayo Trough, which include thrusting associated with footwall shortcut faults and clockwise rotation. Although the timing of deformation overlaps that of diorite sill emplacement, it remains poorly constrained in absolute terms.

A Pennsylvanian echinoid from County Clare, western Ireland

A Pennsylvanian echinoid from County Clare, western Ireland

Vitamin D and celiac disease.

Celiac disease (CD) is an immune-mediated condition affecting the small intestine. Its reported global prevalence falls within the range of 0.7% to 1.4%. Notably, historically, higher rates, reaching 1% in Western Ireland, have been documented. Recent research has even revealed prevalence rates as elevated as 2% in northern Europe. These findings underscore the urgency for swift and cost-effective diagnosis, especially in individuals identified through screening efforts. At present, the diagnosis of CD relies on a multifaceted approach involving positive serological markers such as IgA anti-tissue transglutaminase (anti-TTG) and anti-endomysial antibodies (anti-EMA). These serological findings are assessed in conjunction with classical histological alterations, as outlined in the Marsh classification. CD is an inflammatory condition triggered by the consumption of gluten, resulting from intricate interactions between genetic, immunological, and environmental factors. CD is linked to malabsorption, leading to nutritional deficiencies. Individuals with CD are required to adhere to a gluten-free diet, which itself can lead to nutrient deficiencies. One such deficiency includes vitamin D, and there is substantial experimental evidence supporting the notion of a bidirectional relationship between CD and vitamin D status. A low level of vitamin D has a detrimental impact on the clinical course of the disease. Here we summarize the key characteristics of CD and explore the prominent roles of vitamin D in individuals with CD.

Insights into the morphology of Sphenothallus (Cnidaria): New features identified in an old genus, from the Carboniferous (Pennsylvanian, Bashkirian) of western Ireland

Insights into the morphology of Sphenothallus (Cnidaria): New features identified in an old genus, from the Carboniferous (Pennsylvanian, Bashkirian) of western Ireland

New Data on the Ben Levy Anticline, With Reverse Reactivation on the Coolin Fault Giving Up-Side-Down Silurian Rocks in Joyces Country, Connemara

In Joyces Country in western Ireland, the ~ E-W striking axial plane of the D7 Ben Levy anticline folded Dalradian and unconformable Silurian rocks. The north side of the fold is mostly coincident with the contemporaneous ESE-WNW striking steeply dipping Coolin Fault close to the Dalradian-basal Silurian contact. This fault was first initiated by sinistral strike-slip motion, probably of Ordovician age, and then secondly used by post-Wenlock down throw on the north side to form the D7 anticline. Later a newly recognised third motion of reverse reactivation on the fault uplifted the north limb of the fold and locally overturned the Silurian succession against irregularities in the SSW dip of the fault. Still later, but pre-374Ma (Devonian), NE-SW faulting exposed different previous levels of the Coolin Fault zone. The superb Dalradian rock exposure provides details not generally apparent on other reactivated faults in Connemara.

Structural Control of the Deposition and Subsequent Deformation of the Killary Habour-Joyce Country Silurian Succession

The Killary Harbour–Joyce Country succession of Silurian rocks forms the remnant of one of three successor basins that developed after the Grampian Orogeny and which are preserved in western Ireland. Its outcrop everywhere obscures the contact between the Dalradian rocks of Connemara and the Ordovician rocks of the South Mayo Trough. The outcrop is bisected by the prominent north-west trending Maam Valley Fault Zone that is shown to have a component of synsedimentary movement down-throwing to the southwest. The original basin margins lay well outside the current outcrop area. North-south shortening and inversion of the basin was accomplished by reactivation and inversion of earlier fault structures and was exclusively in the brittle zone. Folding of the Silurian rocks with weak to moderate cleavage and deformation of the basement rocks of Connemara was accomplished by largely vertical movements along numerous strike parallel and oblique fractures. Break-back inversion structures were developed along the southern margin of the South Mayo Trough, which include thrusting associated with footwall shortcut faults and clockwise rotation. Although the timing of deformation overlaps that of diorite sill emplacement, it remains poorly constrained in absolute terms.

Saint Patrick, what about legless lizards? Tracing the mitochondrial affiliation and possible origin of Anguis (Squamata: Anguidae) population in Ireland

Abstract Ireland is home to only one native lizards’ species, the viviparous lizard and one of the few areas of the world where there are no native snakes. However, since the end of the 19th century, there have been reports indicating the presence of legless lizards, Anguis fragilis Linnaeus, 1758 population whose origin is unknown. As this genus has been intensively studied genetically and taxonomically in the past decade, we have decided to investigate newly obtained mtDNA sequences (ND2 gene) to trace affiliation of the Anguis population in Ireland. We genetically examined 12 individuals from the Burren area (western Ireland) and compared them to available data. The Irish population was identified as belonging to A. fragilis, specifically to the most common haplotype of the Illyrian-Central European haplogroup. This genetic affiliation is shared by populations across a wide European region, including the western Balkans, Spain, France, western Hungary, Austria, Germany, Latvia, Poland, the Czech Republic, Russia, Sweden, Norway, and Great Britain. Our findings showed that the Irish population does not exhibit unique mitochondrial variability. This haplotype is present across the range of the species, including Great Britain, which is not in contrast with the earlier hypothesis that nearby populations from this area, along with human-mediated introductions, could be the source of Anguis populations in Ireland. This is thus potentially the first documented case of Anguis introduction out of the native range. However, further research, including broader sampling in Great Britain and genomics, is required to determine and trace its exact origin.