A protocol for the monazite (LREE,Y,Th,U,Si,Ca)PO4 in situ Th-U-Pb dating by electron probe microanalyser (EPMA) involves a suitable reference monazite. Ages of several potential reference monazites were determined by TIMS-U-Pb isotope analysis. The EPMA protocol is based on calibration with REE-orthophosphates and a homogeneous Th-rich reference monazite at beam conditions of 20 kV, 50 nA, and 5 µm for best possible matrix matches and avoidance of dead time bias. EPMA measurement of samples and repeated analysis of the reference monazite are performed at beam conditions of 20 kV, 100 nA, and 5 µm. Analysis of Pb and U on a PETL crystal requires YLg-on-PbMa and ThMz-on-UMb interference corrections. Offline re-calibration of the Th calibration on the Th-rich reference monazite, to match its nominal age, is an essential part of the protocol. EPMA-Th-U-Pb data are checked in ThO2*-PbO coordinates for matching isochrones along regressions forced through zero. Error calculations of monazite age populations are performed by weighted average routines. Depending on the number of analyses and spread in ThO2*-PbO coordinates, minimum errors <10 Ma are possible and realistic for Paleozoic monazite ages. A test of the protocol was performed on two garnet metapelite samples from the Paleozoic metamorphic Zone of Erbendorf-Vohenstrauß (NE-Bavaria, western Bohemian Massif).