This study sought to analyze the relationship between regional body composition, swimming performance, and aerobic and force profile determined through tethered swimming in well-trained swimmers. Eleven male and five female swimmers were involved in the study and underwent the following evaluations: (1) body composition, assessed by the dual-energy X-ray absorptiometry method (DXA); (2) swimming performance, determined for 200, 400, 800, and 1.500 m front-crawl swimming; (3) a tethered swimming force test to determine maximum and mean force (Fmax and Fmean); and (4) an incremental tethered swimming test for the aerobic profile determination of the swimmers. Oxygen uptake (VO2) was directly measured by an automatic and portable system (K4b2 Cosmed, Italy). The fat-free mass (lean mass + bone mineral content, LM+BMC) in lower and upper limbs (UL_LM+BMC: 6.74 ± 1.57 kg and LL_LM+BMC: 20.15 ± 3.84 kg) positively correlated with all indexes of aerobic conditioning level, showing higher coefficients to the indexes representing the ability to perform at high aerobic intensities (VO2max: 49.2 ± 5.9 mL·kg-1·min-1 and respiratory compensation point (RCP): 43.8 ± 6.0 mL·kg-1·min-1), which attained 0.82 and 0.81 (with VO2max), 0.81 and 0.80 (with RCP). The S200 (1.48 ± 0.13 m·s-1) was significantly correlated to Trunk_LM+BMC (r = 0.74), UL_LM+BMC (r = 0.72), Total_LM+BMC (r = 0.71), and LL_LM+BMC (r = 0.64). This study highlights that regional body composition plays an important role in swimming, and body segment analysis should be considered instead of the total body. Tethered swimming may represent a useful method for force and aerobic assessment, aiming at training control and performance enhancement.