Nanotechnology is the most well-known disciplines of science due to its wide range of applications. Nanoparticles have been used commonly for agriculture, electronic, medicine, medicinal material, and sensory. In this study, Prunus laurocerasus L. fruits were heated in distilled water for 2 hours at 40°C. After filtration, the treatment of silver nitrate (1.0 mM) with plant solution yielded silver nanoparticles (AgNPsμ@pl). Advanced spectroscopic techniques elucidated the synthesized AgNPs@pl. The functional moieties of secondary metabolites responsible for capping, reducing and stabilizing agents were determined by Fourier transform infrared spectroscopy (FTIR). The surface plasmon resonance at 468 nm was presented by UV-Vis spectroscopy. Transmission electron microscopy (TEM) displayed the particles as spherical in the size of 15 nm. The structure and particle size were also calculated by X-ray diffraction (XRD) spectroscopy and AgNPsμ@pl were found as cubic structure with the particle size of 17 nm. The zeta potential confirmed the stability of nanostructures. The high negative zeta potential value (-16.5 mV) displayed the dispersion of the nanostructure with stability.