Waterfowl Habitat Research Articles

Challenges and strategies for implementing the Ramsar Convention. Balancing Economic Development and Wetland Conservation

Ramsar Convention on Wetlands of International Importance, particularly focusing on waterfowl habitats, signifies a pivotal shift in global attitudes towards wetland conservation. Historically viewed as hindrances to development, wetlands have suffered from drainage and reclamation efforts, resulting in significant biodiversity loss. However, a growing recognition of their ecological significance has spurred international action. The Convention, established in 1971, serves as a landmark agreement to safeguard wetland ecosystems. It mandates member states to designate and conserve wetlands within their territories, emphasizing the importance of international cooperation due to the transboundary nature of wetland ecosystems. The Convention’s objectives encompass sustainable development goals, including water quality, biodiversity, and climate change mitigation. Implementation relies on national policies and collaboration among stakeholders. Furthermore, the Convention aligns with other environmental agreements, fostering synergies to enhance wetland protection globally. Despite these positive developments, there are significant challenges in ensuring the effective implementation of the Convention, particularly when economic interests conflict with environmental conservation. As highlighted in the case of the Bystroe Canal project in the Danube Delta, balancing development with wetland protection remains a critical issue. This article addresses the question of whether the Ramsar Convention can effectively apply its provisions in such cases, arguing that while it provides a strong framework, its effectiveness is often undermined by competing economic priorities. Without stronger enforcement mechanisms and increased international cooperation, the objectives of the Convention risk being compromised.

Deteriorating wintertime habitat conditions for waterfowls in Caizi Lake, China: Drivers and adaptive measures

China has made enormous strides to achieve high-quality development and biodiversity conservation, and the establishment of nature-protected areas is one of the essential initiatives. Caizi Lake involves a natural reserve and two national wetland parks, accommodating winter migratory waterfowl over the middle and lower Yangtze River basin in China. However, the water transfer from the Yangtze River to the Huai River (YR-HR water transfer) has modified the winter hydrological conditions of Caizi Lake, negatively affecting wintertime waterfowl habitats. Hence, conserving wintertime waterfowl habitats necessitates knowledge of the dynamical mechanisms behind the impacts of YR-HR water transfer on wintertime waterfowl habitats and adaptive measures. Here we developed a machine learning model, the normalized difference vegetation index, and on-spot observatory datasets such as the spatial distribution of waterfowl species and underwater topography of Caizi Lake. We found that the rising winter water level of Caizi Lake encroaches on winter waterfowl habitat with extremely high suitability. Meanwhile, rising water levels reduced waterfowl food sources. Thus, rising water levels due to YR-HR water transfer deteriorated waterfowl living conditions over Caizi Lake. Therefore, we proposed adaptive measures to alleviate these negative effects, such as water level regulation, artificial feeding of waterfowls, restoration and reconstruction of contiguous mudflats, grass flats. This study highlights human interferences with waterfowl habitats, necessitating biodiversity conservation at regional scales.

Beyond the selective regulation of wetlands: towards a rights of wetlands

ABSTRACT The importance of wetlands as places worthy of protection has been recognised since at least 1975, with the passage of the global wetland treaty, the Ramsar Convention (officially the Ramsar Convention on Wetlands of International Importance Especially as Waterfowl Habitat). However, over the past 50 years wetlands have declined and at least 35% of natural wetlands have been lost with more at risk of extinction. Unfortunately, wetland habitats continue to suffer, despite the existence of protection regimes. In this context, this paper invites us to push the way we think about current wetland regulation and protection. Contemporary conceptions of wetlands that underpin dominant protection and management regimes tend to characterise wetlands in a way that overlooks all essential elements of the ecosystem. Our concern is to illuminate the plight of wetland ecosystems as interconnected waterscapes requiring more than a selective or partial regulative protection effort. We argue for a ‘rights of wetlands’ framing for a more holistic approach to regulate the management of these key ecosystems. We situate our argument using a Ramsar-listed wetland example, the Towra Point Nature Reserve, located in Botany Bay (Kamay), Sydney, Australia. Ultimately, we invite a recalibration of regulatory efforts for wetland conservation.

Habitat quality evaluation and pattern simulation of coastal salt marsh wetlands

Coastal salt marsh wetlands not only sequester a large amount of organic carbon, mitigating the effect of climate change, but also nurture rich wetland resources and diverse ecological environments. In this study, habitat pattern and quality of the Jiangsu Yancheng Wetland Rare Birds National Nature Reserve were studied. The evolution of habitat patterns was analyzed using the U-Net model and Sentinel-2 data. The habitat quality was evaluated using the InVEST model, while the future habitat pattern in 2027 under different scenarios were simulated using the PLUS model. Our results showed that, during 2017–2022, the Suaeda salsa habitat showed a net decrease in area of 2077.61 ha, while Spartina alterniflora and Phragmites australis habitats manifested a net increase in different degrees. The overall habitat pattern was characterized by fragmentation decline and regularization enhancement. The habitat quality decreased from 0.75 to 0.72, mainly due to the loss of the S. salsa habitat and the expansion of the P. australis habitat. The simulation results indicated that, the habitat quality is expected to further decline to 0.71 under the natural development scenario, and 390.27 ha of S. salsa habitat will convert to P. australis. While in government control scenario, the habitat quality is expected to improve to 0.78, which was 0.07 higher than that in natural development scenario, and S. salsa habitat can be restored well. This study provides a scientific basis for the protection of suitable habitats for waterfowl and is crucial for the ecological conservation and management planning of nature reserves and coastal salt marsh wetlands.

Extreme drought alters waterfowl distribution patterns and spatial niches in floodplain wetlands

The Yangtze River Basin was among the areas most severely affected by the widespread extreme drought in the Northern Hemisphere in 2022. Drought conditions in this basin extended into the wintering season, significantly influencing waterfowl habitat availability. Exploring how waterfowl distributions and ecological niches respond to these changes in wetland environmental conditions and the underlying ecological mechanisms are important for biodiversity conservation and understanding wetland ecological processes. By integrating field observations, remote sensing, and ecological niche modelling, we analysed habitat changes over the past decade and investigated how extreme drought affected the distribution patterns and spatial niches of two waterfowl guilds, ducks and geese, in the Dongting Lake Region, a key wetland ecosystem in the Yangtze River Basin. The extreme drought led to a drastic reduction in the waterbody area of natural habitats and caused vegetation to enter the wilting stage prematurely. In response, a substantial proportion of waterfowl shifted to adjacent managed wetlands with stable water levels, especially the ducks, whose distribution pattern was fundamentally altered. The proportion of both waterfowl guilds in the managed wetland showed a significant correlation with the proportion of the waterbody area. Further, we compared the spatial niche of waterfowl in 2022 with that in the former year, which represented a typical year with normal hydrology. The spatial niche of ducks underwent a substantial contraction, while that of geese remained large in both years but shifted inward in 2022 towards areas with lower elevation, and foraging resource use was altered. Therefore, for both waterfowl guilds, the extent of habitat overlap between the two years was small. This study has important implications for understanding the impact of extreme climatic and hydrological events on wetland ecosystems. Furthermore, our results emphasise the importance of water level-managed wetlands for biodiversity conservation.

Potential use of poultry farms by wild waterfowl in California's Central Valley varies across space, times of day, and species: implications for influenza transmission risk

Interactions between wildlife and livestock can lead to cross‐species disease transmission, which incurs economic costs and threatens wildlife conservation. Wild waterfowl are natural hosts of avian influenza viruses (AIVs), are often abundant near poultry farms, and have been linked to outbreaks of AIVs in poultry. Interspecific and seasonal variation in waterfowl movement and habitat use means that the risk of disease transmission between wild birds and poultry inevitably varies across species, space, and time. Here, we used GPS telemetry data from 10 waterfowl species in and near California's Central Valley, a region where both wild waterfowl and domestic poultry are abundant, to study selection of poultry farms by waterfowl across diel, seasonal, and annual cycles. We found that waterfowl selected for wetlands, open water, protected areas, and croplands, which meant that they generally avoided habitats that were likely to be used for poultry farming. These selection patterns were linked to species' ecology and diel behavioral patterns, such that avoidance of poultry habitats was stronger for local or partial migrants than for long‐distance migrants, and stronger during daytime than at night. We then combined these habitat selection results with data on poultry farm locations to map risk of waterfowl–poultry contact across the Central Valley. Average selection strength at poultry farms was low, suggesting that current placement of poultry farms is generally effective for limiting risk of contact with wild birds. When we combined these habitat selection results with data on species' abundances and AIV infection prevalence, we found dramatic variation in potential AIV transmission risk among species. These results could be used to prioritize surveillance and biosecurity efforts for regions and times of relatively high risk. More generally, these results highlight that fine‐scale movement data can help identify interspecific, seasonal, and diel patterns in animal behaviors that affect wildlife and poultry health.

What waterfowl hunters want: exploring heterogeneity in hunting trip preferences

Canadian and American waterfowl hunters were surveyed to identify their hunting trip preferences. Respondents were individuals that were now participating or had participated in waterfowl hunting, and most had hunted the majority of the last five years. We identified four latent classes of waterfowl hunters that varied in their preferences for harvest, access effort, length of travel, quantity of waterfowl seen, and the potential for interference/competition. We found a diminishing return associated with the number of waterfowl harvested, and that ‘devoted’ and ‘local’ hunters did not perceive appreciable benefit from harvesting more birds beyond harvesting a single bird. Results highlight the importance of not only considering population size, but also the location of habitat for people and waterfowl. Our results provide waterfowl managers important insights into the heterogeneity of North American waterfowl hunters by highlighting differences in priorities for waterfowl hunting trips. Notably, to address this heterogeneity, managers could consider the balance of objectives, actions and resources designed to satisfy current waterfowl hunters. Managing access to improve the likelihood that hunters will see and have opportunities to harvest some waterfowl has benefit to hunters.

Economic and Environmental Implications of Wood Exploitation in the Mangrove Ecosystem in Tiko, South West Region of Cameroon

Mangroves are vital ecosystems performing myriad functions and providing critical ecological goods and services. However, over the years, population growth and land use mutations have significantly reduced the ecological capacity of mangroves. This study aimed at identifying the economic and environmental implications of wood exploitation in the mangrove ecosystem. Data were collected from the field through the administration of questionnaires and field observations. This study examined the different drivers of wood exploitation which have resulted in the gradual destruction and degradation of mangrove ecosystem. Anthropogenic activities relating to the utilization of mangroves have significantly reinforced the rate of degradation within the study area. The results also show that the exploitation of mangrove forest for wood and fishery resources, fish smoking and charcoal production have also contributed to the daily and monthly income of the exploiters, making them essential to poverty alleviation. However, mangrove exploitation for various economic motives identified in the study have contributed enormously to ecological disequilibrium which has aggravated mangrove degradation. Some of the environmental consequences identified during field observations include loss of biodiversity, reduction in fish quantity and water pollution amongst others. To address these issues, it is necessary to promote environmental education, carry out reforestation, and establish community corporation in the wise use of mangroves. This falls in line with Cameroon’s 1994 Forestry and Environment Law relating to sustainable exploitation of forest resources and 1972 Ramser Convention on Wetlands as Water Fowl Habitats.

Marginal value analysis reveals shifting importance of migration habitat for waterfowl under a changing climate.

Migratory waterfowl are an important resource for consumptive and non-consumptive users alike and provide tremendous economic value in North America. These birds rely on a complex matrix of public and private land for forage and roosting during migration and wintering periods, and substantial conservation effort focuses on increasing the amount and quality of target habitat. Yet, the value of habitat is a function not only of a site's resources but also of its geographic position and weather. To quantify this value, we used a continental-scale energetics-based model of daily dabbling duck movement to assess the marginal value of lands across the contiguous United States during the non-breeding period (September to May). We examined effects of eliminating each habitat node (32 × 32 km) in both a particularly cold and a particularly warm winter, asking which nodes had the largest effect on survival. The marginal value of habitat nodes for migrating dabbling ducks was a function of forage and roosting habitat but, more importantly, of geography (especially latitude and region). Irrespective of weather, nodes in the Southeast, central East Coast, and California made the largest positive contributions to survival. Conversely, nodes in the Midwest, Northeast, Florida, and the Pacific Northwest had consistent negative effects. Effects (positive and negative) of more northerly nodes occurred in late fall or early spring when climate was often severe and was most variable. Importance and effects of many nodes varied considerably between a cold and a warm winter. Much of the Midwest and central Great Plains benefited duck survival in a warm winter, and projected future warming may improve the value of lands in these regions, including many National Wildlife Refuges, for migrating dabbling ducks. Our results highlight the geographic variability in habitat value, as well as shifts that may occur in these values due to climate change.

Evaluation of breeding distribution and chronology of North American scoters

North America's scoter species are poorly monitored relative to other waterfowl. Black Melanitta americana, surf M. perspicillata, and white‐winged M. deglandi scoter abundance and trend estimates are thus uncertain in many parts of these species' ranges. The most extensive source of waterfowl abundance and distribution data in North America is the Waterfowl breeding population and habitat survey (WBPHS). Although the WBPHS effectively monitors most species, both its timing and geographic coverage may preclude accurate scoter monitoring. Therefore, our goal was to better define when and where scoters breed to help interpret survey results and optimize potential supplemental survey efforts for scoters. We integrated satellite telemetry tracking data from scoters marked at multiple molting, staging, breeding, and wintering areas along the Atlantic and Pacific coasts to quantify continent‐wide breeding chronology and distribution. We also examined possible drivers of variation in timing of arrival, length of stay, and departure at nesting locations. We documented a northwest to southeast distribution of estimated breeding sites across Alaska and Canada. On average, scoters arrived at nest sites on 1 June. Surf scoters and Pacific black scoters arrived earliest and departed earliest. Pacific‐wintering black and white‐winged scoters began breeding earlier than Atlantic‐wintering birds. Additionally, birds arrived at nesting locations earlier in years with earlier snowmelt, and later snowmelt reduced lengths of stay for males. Breeding chronology also varied by age group, with adults arriving earlier than subadults. Our study is the first to comprehensively describe spatial variation in timing of breeding of both Atlantic and Pacific populations of all three scoter species across North America. Our results increase our understanding of how current surveys enumerate scoters and will inform possible supplemental efforts to improve continental monitoring of scoter populations.

Drivers of hydrologic budgets in small terminal lakes in the Alberta prairies

The prairies and boreal plain within the North Saskatchewan Watershed (NSW) of Central Alberta have numerous shallow ponds and lakes that sustain unique aquatic ecosystems and are critical habitat for migratory waterfowl in North America. However, over the past 20 years water levels have declined and the reasons are unresolved. Here we used a combination of inorganic geochemical analyses and stable water isotopes to constrain the hydrologic budgets of six lakes in the NSW. Our results show that the bedrock groundwater major element geochemistry is controlled by chemical weathering reactions along the flow paths and is dominated by lower δ18O and δ2H values (i.e. isotopically depleted), while the lake water generally shows unchanging evaporatively enriched stable isotope values and cation concentrations. An isotopic mass balance (IMB) technique combined with solution geochemical modelling using activity – activity plots reveals that deep groundwater input is negligible, while the lakes appear to lose a greater fraction of water inflows to evaporation (60%) than shallow groundwater and surface outflow (40%). The relative importance of shallow groundwater requires further study, as shallow groundwater sampling locations are scarce and surface outflow is negligible. The IMB technique also indicated that these prairie lakes have short water residence times, ranging from 1.8 to 10.4 yrs. Our results suggest that declining lake levels are likely the result of a changing relationship between precipitation and evaporation from the climatic norm.

An agent-based model to quantify energetics, movement and habitat selection of mid-continent mallards in the Mississippi Alluvial Valley

The dynamics of wintering waterfowl populations at the landscape scale are the result of complex interactions of environmental, behavioral and energetic drivers. Agent-based models provide a method to directly link these factors in a spatially explicit framework and allow the emergence of patterns from the aggregation of individual agent actions. We adapted the Spatially-explicit Waterbird Agent-based Model Program (SWAMP), originally developed for waterfowl in central California, to simulate a basin-scale population of mallards (A. platyrhynchos) wintering in the Mississippi Alluvial Valley over a four-month period (November–February). Simulated agents move within the landscape, foraging on areas made available based on a probabilistic inundation status, and converting food resources to endogenous energy. The model uses a high-resolution map of eastern Arkansas waterfowl habitats and incorporates a hierarchical habitat selection system that enables mallards to relocate at increasing scales in response to changing food availability. We validated the performance of modeled mallard body condition and behavioral metrics under a range of environmental conditions against expected outcomes derived from empirical data and found that the simulation produced realistic representations of changes in flight distances, energy expenditure, lipid storage, and foraging habitat use in response to depleting food resources over time. We discuss the model's applicability as a tool to quantify waterfowl response to a range of environmental conditions and to evaluate scenarios of landscape composition and configuration in the context of waterfowl population management.

Evaluation of fall‐seeded cover crops for grassland nesting waterfowl in eastern South Dakota

AbstractThe Prairie Pothole Region (PPR) is the primary breeding ground for many species of North American waterfowl. The PPR was historically dominated by mixed and tallgrass prairies interspersed with wetlands, but >70% of the native grassland area has been lost due to widespread conversion to croplands. Cover cropping is a reemerging farming technique that may provide suitable nesting cover for grassland nesting waterfowl in active croplands, but waterfowl nest survival in fall cover‐cropped fields has not been evaluated. We studied use (nest abundance and density) and nest survival of breeding waterfowl in fall‐seeded cover crops and perennial cover during 2018 and 2019. We searched 2,094 ha of cover crops and 1,604 ha of perennial cover and found 123 and 304 duck nests, respectively, in each cover type. Estimated nest success (34‐day interval) was 3.7% and 16.6% in cover crops during 2018 and 2019, respectively, versus 22.1% in 2018 and 24.9% in 2019 in perennial cover, with increased success of cover‐crop fields in 2019 resulting from precipitation that prevented most fields from being planted to row crops. In a model that included effects of planting, daily nest survival in perennial cover was 0.944 (SD = 0.026) in 2018 and 0.960 (SD = 0.019) in 2019. Estimated daily nest survival was 0.912 (SD = 0.040) in 2018 and 0.960 (SD = 0.019) in 2019 during intervals when planting did not occur, but was only 0.417 (SD = 0.124) in 2018 and 0.612 (SD = 0.117) in 2019 on the day that planting occurred. Estimated nest densities in 2018 and 2019, adjusted for nests that failed prior to discovery, were 5.1 (SE = 1.1) and 11.0 (SE = 3.1) nests 100‐ha−1 in perennial cover, but only 2.1 (SE = 0.8) and 2.6 (SE = 0.7) in cover crops, respectively. Based on observed nest initiation and planting dates, about 70% of duck nests in cover crops would experience planting events in a typical growing season. Our results suggest that under current management techniques, fall‐seeded cover crops offer poor nesting habitat for waterfowl; however, the important benefits cover crops provide to soil health, water quality, and other ecosystem services remain.

Marine Endangered and Threatened Species in Russia: A Review of Current Conservation Strategies and Management Legislative Tools

Despite the global decline in marine species biodiversity, relatively few countries have enacted national endangered and threatened species legislation. Tailoring an adequate legislative framework with clear objectives and regulations consistent with the available scientific evidence is fundamental for the effective conservation of marine endangered and threatened species. This paper analyzes the legal framework and current institutional tools for the conservation of marine endangered and threatened species in the Russian Federation. In this regard, important legislative tools include federal laws, as well as internationally binding signed agreements, among others, such as the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) and the Convention on Wetlands of International Importance Especially as Waterfowl Habitat (Ramsar). A strategy and an action plan for the conservation of biological diversity were also developed. Besides, the most important tool for the conservation and protection of marine endangered and threatened species is the Red Book of the Russian Federation (RBRF) and other regional Red Books. Responsibility for causing harm to the species listed in the RBRF and their habitat is specified in the code of administrative offenses and the criminal code of the Russian Federation. Finally, conclusions and identified gaps were highlighted in the last section, including, among other things, that legislation is still limited in how it takes the impacts of climate change into account. Such type of study is highly recommended, considering the relatively few number of papers dedicated to the study of the impact and/or implications of the conservation tools and strategies mentioned in this paper on the status of the marine endangered and threatened species.

Semantic Segmentation with Multispectral Satellite Images of Waterfowl Habitat


 
 
 Migratory waterfowl (i.e., ducks, geese, and swans) management relies on landscape bioenergetic models to inform on-the-ground habitat conditions and conservation practices. Therefore, conservation planners rely on accurate predictions of wetland habitats for waterfowl at regional scales. Unharvested flooded corn is a popular management tool on public and private lands that greatly increases landscape-level energy compared to other wetlands; thus, landscape bioenergetic models are particularly sensitive to these habitat features. Despite their importance to conservation planning and implementation, the abundance and distribution of unharvested flooded corn fields across North America is unknown. Furthermore, training data is difficult to collect and accurate predictions are challenging given their unique attributes and discreteness at landscape-level lens. Advances in multispectral imagery and deep learning algorithms may enable continuous and autonomous detection of these habitat features. Therefore, we conducted modeling experiments using training data of unharvested flooded corn fields in West Tennessee and multispectral imagery collected from Sentinel-2 satellite missions. We performed several experiments using individual band combination composites and/or vegetation indices to identify optimal bands using MRUNET architectures. We subsequently used 3 ensemble models of important individual networks. We found the use of multispectral bands was necessary and although the CIR composite and OSAVI index improved precision, the 12-band composite increased recall, the metric we were most interested in. Moreover, all ensembles exhibited poor performance. Here, we present results of our initial modeling experiments and suggest future modeling exercises including temporal image and vegetation index stacking using multi-modal and/or recurrent neural network architectures.
 
 

Waterfowl distribution and productivity in the Prairie Pothole Region of Canada: tools for conservation planning

AbstractSpecies conservation requires an understanding of the factors and interactions affecting species distribution and behavior, habitat availability and use, and corresponding vital rates at multiple temporal and spatial scales. Opportunities to investigate these relationships across broad geographic regions are rare. We combined long‐term waterfowl population surveys, and studies of habitat use and breeding success, to develop models that identify and incorporate these interactions for upland‐nesting waterfowl in the Prairie Pothole Region (PPR) of Canada. Specifically, we used data from the annual Waterfowl Breeding Population and Habitat Survey (1961–2009) at the survey segment level and associated habitat covariates to model and map the long‐term average duck density across the Canadian PPR. We analyzed nest location and fate data from approximately 25,000 duck nests found during 3 multi‐year nesting studies (1994–2011) to model factors associated with nest survival and habitat selection through the nesting season for the 5 most common upland nesting duck species: mallard (Anas platyrhynchos), gadwall (Mareca strepera), blue‐winged teal (Spatula discors), northern shoveler (Spatula clypeata), and northern pintail (Anas acuta). Duck density was highly variable across the Canadian PPR, reflecting positive responses to local wetland area and count, and amounts of cropland and grassland, a regional positive response to latitude, and a negative response to local amounts of tree cover. Nest survival was affected by temporal and spatial variables at multiple scales. Specifically, nest survival demonstrated interactive effects among species, nest initiation date, and nesting cover type and was influenced by relative annual wetness, population density, and surrounding landscape composition at landscape scales, and broad geographic gradients (east‐west and north‐south). Likewise, species‐specific probability of nest habitat selection was influenced by timing of nest initiation, population density, relative annual wetness, herbaceous cover, and tree cover in the surrounding landscape, and location within the Canadian PPR. We combined these models, with estimates of breeding effort (nesting, renesting, and nest attempts) from existing literature, in a stochastic conservation planning model that estimates nest distribution and success given spatiotemporal variation in duck density, habitat availability, and influential covariates. We demonstrate the use of this model by examining various conservation planning scenarios. These models allow estimation of local, landscape, and regional influence of conservation investments and other landscape changes on the productivity of breeding duck populations across the PPR of Canada. These models lay the groundwork for the incorporation of conservation delivery costs for full return‐on‐investment analyses and scenario analyses of climate, habitat, and land use change in regional and continental population models.

Habitat suitability and potential biological corridors for waterbirds in Yancheng coastal wetland of China

Yancheng coastal wetland, as an important part of the East Asian–Australasian Flyway, provided habitat for more than 200 migratory waterbirds by rest and over-winter. After it was listed as a “World Heritage List” in 2019, the protection of waterfowl habitat in Yancheng coastal wetland has attracted extensive attention from the international community. As an indicator species of Yancheng coastal wetland ecosystem health, waterbirds have practical significance for the protection of Yancheng coastal wetlands. Due to the long-term impact of human activities, the waterbird habitats are facing the threat of habitat fragmentation and lose, posing a serious challenge to its survival and diversity. Based on the survey data obtained Gruiformes, Charadriiformes, and Anseriformes waterbirds during the whole years from 2017 to 2021, this paper used the Maxent software for modeling species niches and distributions, a comprehensive evaluation method based on fuzzy mathematics, and Linkage Mapper model to assess the habitat suitability distribution of waterbirds, identify protection gaps and potential biological corridors for waterbirds in Yancheng coastal wetland. The results showed that habitat suitability and anthropogenic disturbance were the decisive factors affecting habitat selection of waterbirds. The suitable habitats for Gruiformes, Charadriiformes, and Anseriformes waterbirds were 23868, 22670, and 24870 ha, respectively. The suitable habitat area of waterbirds was 121,930 ha, concentrated on the study area's central and southern coast. Sheyang estuary was identified as a vital conservation gap in the study area. Meanwhile, biological corridors of a total of 33 waterbirds were identified in the study area. The longest biological corridor was 18.78 km, and the shortest was 0.09 km. Our research can provide scientific support for Yancheng coastal wetland protection and the improvement of waterbird diversity, together with the achievements of an ecological and sustainable situation between environmental conservation and economic development.

Intestinal Microbiota of Anser fabalis Wintering in Two Lakes in the Middle and Lower Yangtze River Floodplain.

The intestinal microbiota of migratory birds participate in the life activities of the host and are affected by external environmental factors. The difference in habitat environment provides diversity in external environmental selection pressure for the same overwintering waterfowl, which may be reflected in their intestinal microbiota. Caizi lake and Shengjin Lake in the Middle and Lower Yangtze River Floodplain are the main habitats for migratory waterfowl in winter, especially the Anser fabalis (A. fabalis). It is important to explore the changes in intestinal microbiota composition and function of A. fabalis in the early overwintering period to clarify the effect of habitat size and protection status on intestinal microbiota. In this study, the composition and structural characteristics of the intestinal microbiota of A. fabalis in Shengjin Lake (SL) and Caizi Lake (CL) were preliminarily explored in order to obtain data for the migratory birds. In both SL and CL groups, 16S rRNA amplicon sequencing analysis showed that Firmicutes was the dominant bacterial phylum, but the relative abundance showed significant differences. Lactobacillus was the most abundant genus in both SL and CL groups. At the species level, the abundance of L. aviaries was the highest, with a relative abundance in both SL and CL groups of more than 34%. When comparing the average relative abundance of the 15 most abundant genera, it was found that Subdoligranulum, Exiguobacterium, and Terrisporobacter had higher abundances in the intestinal microbiota of CL A. fabalis, while Streptococcus and Rothia had higher abundances in the intestinal microbiota of SL A. fabalis. There was only a positive correlation between Bacteroidota and Proteobacteria in the intestinal microbiota flora of SL A. fabalis, and the species were closely related. At the same time, there were positive and negative correlations between Firmicutes and Actinomycetes. However, CL is mainly associated with a positive correlation between Firmicutes and Actinomycetes, and there are also a small number of connections between Firmicutes. PICRUSt1 prediction analysis revealed that the Clusters of Orthologous Groups (COG) functions of SL and CL involve energy production and transformation, amino acid transport and metabolism, carbohydrate transport and metabolism, and transcription. Understanding the changes in intestinal microbiota in Aves during the overwintering period is of great importance to explore the adaptation mechanism of migratory Aves to the overwintering environment. This work provides basic data for an A. fabalis intestinal microbiota study.

Tribally-Led Consultation on the Ethical Use of Sedimentary Ancient DNA for a Culturally Important Plant: Manoomin, Psin, Zizania (Wild Rice)

Led by Native American resource managers, we have convened a working group across 20+ tribal entities in the north-central United States, with the goal of building consensus around the use of sedimentary ancient DNA (sedaDNA) to detect deep-past and recent populations of wild rice (manoomin in Ojibwe, psiŋ in Dakota, Zizania palustris and Z. aquatica) using lake sediment cores. Wild rice is of extremely high cultural and spiritual importance to many Indigenous people of the Laurentian Great Lakes region, as well as being a valuable traditional food and providing important habitat for waterfowl and other organisms. Because of its importance, any research involving wild rice must be tribally led, and outcomes designed to benefit the tribes (e.g., Matson et al. 2020). Wild rice is threatened by environmental degradation due to industrial processes and agriculture, so its protection can be a contentious regulatory and political issue in Minnesota, Wisconsin, and Michigan, involving tribal, state, and federal agencies. The wild rice paleorecord has tremendous potential for detecting and tracking past changes in wild rice distributions. Until recently, however, proxies for wild rice were either ineffective (pollen, seeds), or low-throughput and thus not scalable to region-wide use (phytoliths). SedaDNA would supplement Indigenous knowledge and Western scientific methods to inform conservation, management, designation for protection, and the enhancement of cultural and historical records. In this presentation, we will describe how we are synthesizing output from facilitated online meetings with and presentations to tribal resource managers into a white paper providing guidance from these sovereign Nations to academic researchers, agencies, and policymakers on restrictions on the utilization of sedaDNA of wild rice, the leadership roles tribal entities should take in all research, and tribal priorities for the application of this technique.

Persistence of low pathogenic avian influenza virus in artificial streams mimicking natural conditions of waterfowl habitats in the Mediterranean climate

Avian influenza viruses (AIVs) can affect wildlife, poultry, and humans, so a One Health perspective is needed to optimize mitigation strategies. Migratory waterfowl globally spread AIVs over long distances. Therefore, the study of AIV persistence in waterfowl staging and breeding areas is key to understanding their transmission dynamics and optimizing management strategies. Here, we used artificial streams mimicking natural conditions of waterfowl habitats in the Mediterranean climate (day/night cycles of photosynthetic active radiation and temperature, low water velocity, and similar microbiome to lowland rivers and stagnant water bodies) and then manipulated temperature and sediment presence (i.e., 10–13 °C vs. 16–18 °C, and presence vs. absence of sediments). An H1N1 low pathogenic AIV (LPAIV) strain was spiked in the streams, and water and sediment samples were collected at different time points until 14 days post-spike to quantify viral RNA and detect infectious particles. Viral RNA was detected until the end of the experiment in both water and sediment samples. In water samples, we observed a significant combined effect of temperature and sediments in viral decay, with higher viral genome loads in colder streams without sediments. In sediment samples, we didn't observe any significant effect of temperature. In contrast to prior laboratory-controlled studies that detect longer persistence times, infectious H1N1 LPAIV was isolated in water samples till 2 days post-spike, and none beyond. Infectious H1N1 LPAIV wasn't isolated from any sediment sample. Our results suggest that slow flowing freshwater surface waters may provide conditions facilitating bird-to-bird transmission for a short period when water temperature are between 10 and 18 °C, though persistence for extended periods (e.g., weeks or months) may be less likely. We hypothesize that experiments simulating real environments, like the one described here, provide a more realistic approach for assessing environmental persistence of AIVs.