As one of Mexico's most crucial water storage facilities, the Villa Victoria Reservoir (VVR) supplies water to over six million people residing in the Mexico City Metropolitan Area. In recent years, this water resource has been subjected to significant risks due to several factors, including human population growth, alterations in global climate patterns, excessive resource utilization, and insufficient protective regulations, thereby endangering not only the biocenosis itself, but also the water supply for numerous inhabitants. This study aimed to evaluate the current state of the reservoir through the determination of conventional and emerging pollutants present in the sampling points, as well as embryotoxicity and oxidative damage in Danio rerio embryos exposed to effluents from the VVR. Embryotoxicity was quantified using the General Morphology Score (GMS) and teratogenic index, whereas oxidative damage was assessed based on lipid peroxidation, hydroperoxide content, oxidized proteins, antioxidant enzyme activity, and gene expression. These results revealed the presence of heavy metals, diverse pharmaceutical compounds, and pesticides. In addition, elevated lipid, hydroperoxide, and protein oxidation accompanied by alterations in superoxide dismutase (SOD) and catalase (CAT) enzymatic activity were observed during exposure. GMS resulted in impaired embryo development and teratogenic effects, including pericardial, axial, and skeletal edema. Furthermore, the upregulation of genes associated with apoptotic processes and antioxidant defense reflects a comprehensive response to oxidative stress. The study concluded that pollutants in VVR water induced oxidative damage, modified antioxidant activity, elicited embryotoxicity, and upregulated oxidative damage-related genes. The findings underscore the necessity of undertaking restoration efforts for water sources, as pollution can potentially endanger aquatic organisms and human well-being.
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