Water Retention Characteristics Research Articles

Applying Resistant Starch to Improve the Gel and Water Retention of Reduced-Fat Pork Batter.

Emulsified meat products contain high animal fat content, and excessive intake of animal fat is not good for health, so people are paying more and more attention to reduced-fat meat products. This study investigated the impact of varying proportions of pork back-fat and/or resistant starch on the proximate composition, water and fat retention, texture properties, color, and rheology characteristic of pork batter. The results found that replacing pork back-fat with resistant starch and ice water significantly decreased the total lipid and energy contents of cooked pork batter (p < 0.05) while improving emulsion stability, cooking yield, texture, and rheology properties. Additionally, when the pork back-fat replacement ratio was no more than 50%, there was a significant increase in emulsion stability, cooking yield, hardiness, springiness, cohesiveness, chewiness, and L* and G' values (p < 0.05). Furthermore, resistant starch and ice water enhanced myosin head and tail thermal stability and increased G' value at 80 °C. However, the initial relaxation times significantly decreased (p < 0.05) and the peak ratio of P21 significantly increased from 84.62% to 94.03%, suggesting reduced fluidity of water. In conclusion, it is feasible to use resistant starch and ice water as a substitute for pork back-fat in order to produce reduced-fat pork batter with favorable gel and rheology properties.

Enhanced Resistance to Desiccation Cracking of Polymer-Bentonite Mixtures: An Experimental Investigation of Underlying Mechanisms

Polymers have been shown to enhance the resistance of swelling clay soils to desiccation cracking, a critical property in engineering applications, particularly in waste containment facilities. However, the microscopic and macroscopic mechanisms driving this improvement remain poorly understood. Additionally, the influence of different mixing methods on these mechanisms is not well-established. While dry mixing is more convenient for onsite implementation, wet mixing offers intercalation between clay and polymer, resulting in potentially more durable stabilization outcomes. In this paper, key properties related to desiccation cracking of a polymer-clay mixture were measured. The mixture was synthesised by amending Na-bentonite with sodium carboxymethyl cellulose (Na-CMC) using dry and wet mixing. Soil water retention characteristics curves (SWCC), swelling and shrinkage potential, tensile strength, and pore size distribution by mercury intrusion porosimetry (MIP) were measured for both mixtures and untreated bentonite. Compared to pure bentonite, mixtures were found to have slightly reduced air-entry values, significantly lower swelling and shrinkage potentials and higher tensile strengths. In all experiments, dry mixing exhibited superior performance compared to wet mixing. MIP analysis of the amended mixtures revealed a more porous structure when compared to untreated bentonite.

The effect of mechanical compaction on the soil water retention curve: Insights from a rapid image analysis of micro-CT scanning

Soil hydrological and agricultural properties are highly affected by the soil water retention characteristics, which are closely related to the void size distribution and structure of the soil. Agricultural lands are often faced with the challenge of soil compaction, which alters the size, shape, connectivity, and morphology of soil voids. To determine the water retention curve and other hydrological and agricultural functions of un-compacted and compacted sandy soils, we used micro-CT techniques and image analysis procedures in both two and three dimensions. We found that these techniques were reliable, as they were validated against physical measurements and empirical physically-based models, and provided a relatively simple and fast way to characterize the key features of soil hydrological and agricultural properties. The findings highlight the impact of compaction on the soil void size distribution, resulting in increased water holding capacity, greater water availability for root uptake, and reduced hydraulic conductivity. We also discussed the differences between the two and three-dimensional analyses, highlighting the better ability to characterize soil hydrological and agricultural functions and soil water retention curve using the three-dimensional image analysis approach.

Effect of volumetric deformation on saturation of unsaturated soils under constant suction

The soil–water retention curve (SWRC) represents a fundamental relationship in the study of unsaturated soils, where volumetric deformation plays a crucial role in shaping the water retention characteristics by impacting the pore size distribution. This study conducted a thermodynamic analysis to derive a relationship between the effective stress parameter and the water retention properties of unsaturated soils. Consequently, a streamlined formula was introduced to forecast the impact of volumetric deformation on saturation at constant suction. This formula, incorporating a singular parameter from the effective stress parameters equations, can be seamlessly integrated with any prevailing SWRC model to characterize the influence of volume deformation on the SWRC. The effectiveness of the proposed model was corroborated through seven sets of experimental data spanning various soils, affirming its capability to precisely describe the water retention behavior of deformable soils under deformation.

Eye of the future: Unlocking the potential utilization of hydrogels in intraocular lenses

AbstractHydrogels are distinguished by their exceptional ability to absorb and retain large volumes of water within their complex three‐dimensional polymer networks, which is advantageous for the development of intraocular lenses (IOLs). Their innate hydrophilicity offers an optimal substrate for the fabrication of IOLs that simulate the natural lens' accommodation, thereby reducing irritation and facilitating healing after surgery. The swelling and water retention characteristics of hydrogels contribute to their notable biocompatibility and versatile mechanical properties. However, the clinical application of hydrogels faces challenges, including managing potential adverse postimplantation effects. Rigorous research is essential to ascertain the safety and effectiveness of hydrogels. This review systematically examines the prospects and constraints of hydrogels as innovative materials for IOLs. Our comprehensive analysis examines their inherent properties, various classification strategies, cross‐linking processes, and sensitivity to external stimuli. Additionally, we thoroughly evaluate their interactions with ocular tissues, underscoring the potential for hydrogels to be refined into seamless and biologically integrated visual aids. We also discuss the anticipated technological progress and clinical uses of hydrogels in IOL manufacturing. With ongoing technological advancements, the promise of hydrogels is poised to evolve from concept to clinical reality, marking a significant leap forward in ophthalmology characterized by improved patient comfort, enhanced functionality, and reliable safety.

Effect of Olive Oil Hydrogel as a Fat Replacer in Beef Meatballs.

Meat and meat products are essential sources of dietary saturated fatty acids. However, excessive consumption of meat and meat products may be harmful to human health. The study evaluates the effect of fat replacement with hydrogels (olive oil in water emulsions gelled by gelatine) in meatballs. The effect of replacing fat with different ratios of hydrogel (control, 25 (F25), 50 (F50), 75 (F75) and 100 % (F100)) on the chemical (fatty acids and thiobarbituric acid reactive substances (TBARS)) and physical (cooking loss, diameter reduction, fat retention, water retention, colour and texture analysis) characteristics of the meatballs were analyzed. The fat content of raw meatball samples was reduced from (31.2±2.2) to (10.5±0.4) % in the sample with the highest fat substitution (F100). The energy levels of the F100 samples were almost 56 % lower than of the control group. Monounsaturated fatty acids (MUFAs) represented the dominant group in all substitution rates of the meatballs, followed by saturated fatty acids (SFAs) and finally polyunsaturated fatty acids (PUFAs). Among the raw meatball samples, the highest oxidation occurred in the F50 and F100 groups. However, it was determined that the difference between F25 and F75 and the difference between control and F75 were not statistically significant (p˃0.05). When the cooked samples were compared, the highest thiobarbituric acid (TBA) value was found in the F50 sample, followed by the F100 and F75 samples. The difference between the mean values of springiness and cohesiveness of the samples was not significant (p˃0.05). The hardness value of samples decreased significantly (p˂0.001) with >75 % fat replacement. It can be concluded that the oil replacement rate that may satisfy consumer demand without impairing the product technological and chemical quality should be <75 %. As the fat replacement ratio increases, the SFA content of cooked meatballs decreases, while the MUFA and PUFA contents increase. Considering the positive effects of reducing the intake of SFAs and increasing the intake of unsaturated fatty acids on non-communicable diseases such as cardiovascular diseases, fat replacement in meatballs is important for future developments.

Machine learning facilitates connections between soil thermal conductivity, soil water content, and soil matric potential

Properly understanding the strongly coupled thermal and hydraulic properties of porous media is crucial to interests in different fields, e.g., agriculture, building physics and meteorology. Previous studies generally consider the thermal and hydraulic properties independently, specifically, the soil thermal conductivity curve and the soil water retention characteristics curve as methods to connect such process are unavailable or untested. In an effort to test methods based on machine learning (ML) to effectively integrate the soil thermal conductivity (λ), matric potential (ψ) and water content (θ), 2328 measurements from 43 soils were compiled using ML algorithms. Five ML algorithms, including random forest-RF, gradient boosting decision tree-GBDT, support vector machine-SVM, multiple linear regression-LR and backpropagation neural network-BPNN, based on the balanced dataset with synthetic minority over-sampling technique were trained, validated and tested to establish the λ(ψ) and λ(θ) relationships. Results demonstrate that, ML algorithms perform better than empirically based methods to optimize the thermal and hydraulic relationship. The selection of ML algorithms has a significant influence on the coupling relationship and should be taken into account. GBDT performed superior for small soil hydrothermal datasets (Testing set: Nash-Sutcliff efficiency-NSE = 0.9999 and Root Mean Square Error-RMSE = 0.0144 W m−1 K−1; independent verification: NSE = 0.7122 and RMSE = 0.2726 W m−1 K−1), while BPNN is limited by the amount of data with NSE = 0.9970, RMSE = 0.0790 W m−1 K−1 of Testing set.

Effects of Polymer Conditioner and Nitrogen Fertilizer Application on Nitrogen Absorption and Utilization of Drip-Irrigated Wheat in Arid Areas

Nitrogen (N), an important element for crop growth, has a great impact on dry matter weight and yield. Currently, improving N fertilizer use rate is an urgent problem to be solved in agricultural production in the world. In this field experiment, a self-developed water-soluble polymer material (PPM) with water retention and slow-release characteristics was combined with different doses of N fertilizer (N300 (100% N), PN300 (PPM + 100% N), PN240 (PPM + 80% N), PN180 (PPM + 60% N), CK (no N and PPM)) to analyze the impacts on N uptake and use efficiency of wheat plants. The results showed that the combined application of PPM and N fertilizer significantly improved yield, plant height, biomass, and N uptake and use efficiency of drip irrigated wheat, and the PN240 group had the highest N use rate. In addition, the PN300 group had the highest yield. N use efficiency in the PN240 group was 40.23% higher than that in the N300 group. Therefore, the combined application of PPM and N fertilizer, especially PN240, can reduce the N fertilizer application rate by increasing N use efficiency. This study provides technical reference for improving the N use efficiency of drip-irrigated wheat in arid areas.

Effects of Cement Treatment on Water Retention Behavior and Collapse Potential of Gypseous Soils: Experimental Investigation and Prediction Models

Gypseous soil poses a significant challenge in geotechnical engineering due to its susceptibility to collapse under saturation. This type of soil covers approximately 33% of regions in Iraq, primarily in unsaturated conditions. This study focuses on two types of gypseous soils: one with moderate gypsum content from Karbala city (G1) and the other with high-gypsum content from Tikrit city (G2), to investigate the effects of cement treatment on their water retention behavior and potential for reducing collapse. Different percentages of cement were mixed with both soils to determine the optimum soil–cement mixture for reducing collapse potential (CP) through single oedometer tests. The water retention characteristics and water retention behavior of samples with varying gypsum content and different levels of cement treatment were examined and compared using a controlled-suction oedometer. The soil–water retention curve (SWRC) of these natural and treated gypseous soils was also investigated and compared in both wetting and drying paths. Additionally, multiple pedotransfer functions (PTFs) were assessed to identify or adapt prediction equation(s) for the SWRC of gypseous soil both with and without cement treatment with acceptable accuracy. The results show that adding cement can decrease the CP of gypseous soils; it also affects their SWRC significantly. By making some simple modifications, the PTFs demonstrate acceptable estimations for the water retention curve of both natural and cement-treated gypseous soils.

Experimental and modeling study of shear strength of unsaturated fine-grained soils with bi-modal water-retention characteristics

The mechanical behavior and strength characteristics of unsaturated fine-grained soils with dual-porosity are of crucial importance in geotechnical designs. Nanyang fine-grained soils have been selected as typical dual-porosity structure soils to perform experimental tests under a wide range of suction and different initial densities while studying its stress-strain-strength properties constitute the main scope of this study. Axial translation and vapor equilibrium techniques are jointly employed to apply a wide suction range. Our data suggest that soil behavior transits from strain-hardening with shear-induced contraction to strain-softening with shear-induced dilation as suction and density increase. By exploiting a bi-modal soil–water retention curve (SWRC) explicitly separating capillarity and adsorption mechanisms, the shear strength is allowed to be analyzed in the capillary suction stress-shear stress space. The strength envelop exhibits bi-linear characteristics. Based on these findings, we propose a bi-linear shear strength criterion specifically for dual-porosity fine-grained soils. We utilize the obtained test data to evaluate existing strength criteria based on effective stress and dual stress variables that consider the bi-modal SWRC characteristics. The comparison indicates that the proposed bi-linear shear strength criterion can more reasonably represent the variation of shear strength under a wide range of suction for unsaturated dual-porosity fine-grained soils.

Improving the Physical Properties and Water Retention of Sandy Soils by the Synergistic Utilization of Natural Clay Deposits and Wheat Straw

Improving the physical properties and water retention of sandy soils is of critical importance in arid and water-scarce regions such as Saudi Arabia. The impacts of organic amendments of different particle sizes coupled with natural clay deposits on improving the soil physio-chemical characteristics, nutrient availability, and growth of Sudan grass were investigated in this study. A loamy sand soil was amended with natural clay deposits at 2.5%, 5.0%, and 10% (w/w) application rates, and in combination with 1.0% (w/w) wheat straw of different particle sizes. The water infiltration, evaporation, and retention characteristics of the amended soil were studied for 9 weeks, and then Sudan grass was grown for 7 weeks. The impacts of the particle size of wheat straw on soil properties and nutrient availability were significant (p &lt; 0.05) when combined with clay deposits. The highest application rate of clay (10%) demonstrated the highest water content (20.63–21.73%), and increased P and K availability to 35.54 and 6980 mg kg−1, respectively, in soil, which were 33% and 88% higher, respectively, compared to the control. Plant N, P, and K concentrations were increased to 0.95%, 0.26%, and 4.33%, respectively, which were 2–3.5-fold higher than the control. Therefore, the integrated application of natural clay deposits and wheat straw of fine particle size could be an effective strategy in improving plant production in water-scarce regions.

Liming leads to changes in the physical properties of acidified forest soils

AbstractBackgroundForest liming is a common practice in many German forests, which aims primarily at improving soil chemical properties that have been negatively impacted by acid rain. Effects on physical functions have not yet been studied widely.AimsThe hypothesis of this study is that chemical changes after liming lead to changes in the physical properties of the humus layer and mineral soil.MethodsWe studied soil physical characteristics (water retention characteristics, air and water permeabilities, and conductivities) of limed and adjacent control plots of sandy to loamy, acidified soils under spruce and beech‐dominated stands.ResultsWe found differences between limed and control plots, especially in the humus layer and mineral top soil. Here, air capacity values (representing air‐filled macropores at −6 kPa) have experienced an increase, while available water capacities (representing the sum of dewatered mesopores between −6 and −1500 kPa) were reduced to a variable extent. These changes in pore size distributions affected gas diffusion as well as gas permeability positively. Unsaturated hydraulic conductivity was not affected. Below the top mineral soil, a tendency to a reduced macroporosity and gas permeability was observed, but no clear changes were detected.ConclusionsSmall but significant changes of soil physical properties and functions in the humus layer and top mineral soil are discussed to be an indirect effect of forest liming. Liming improves pH and nutrient availability, which in turn should have stimulated activity and abundance of soil fauna, especially earthworms. Where observed, their activity could explain the observed changes in soil physical properties. We believe that effects below the top soil might be due to mobilization and translocation of soil particles and a clogging of pores, but these depths were only studied at three plots. Compared to common liming practices, however, the investigated sites are characterized by significantly higher lime applications. The effects on practice liming areas in forestry praxis are therefore likely to be smaller than in this study.

A generalized water retention model in a wide suction range considering the initial void ratio and its verification

A generalized water retention curve (WRC) model in a wide suction range considering the initial void ratio is presented in this paper. Both capillary and adsorbed water in soil pores are considered. Based on the experimental results that the adsorbed water content wa is not dependent on the initial void ratio, a new degree of saturation equation of adsorbed water is established. Meanwhile, a new degree of saturation equation of capillary water at various initial void ratios is proposed with a void ratio-related expression of the air entry value. Compared to Lu’s model, only one parameter b is introduced in the established model, which can be easily calibrated using WRCs under two initial void ratios. The measured WRC results of 5 soils are used to verify the proposed model in comparison with the other 3 widely used WRC models. The soil used in the model validation covers a wide range of soil types from sandy soil to clay soil, including clayey silty sand, sandy clay till, clayey silt, expansive soil and lateritic soil. The proposed model is well verified for its ability to describe soil water retention characteristics in a wide suction range at various initial void ratios.

Degree of compaction, aeration, and soil water retention indices of a sugarcane field without soil disturbance after initial tillage

Soil compaction after initial soil tillage for crop establishment has been a major problem in crop fields because of its deleterious effects on soil functioning and crop performance. Therefore, the study aimed to determine the degree of compaction, soil air capacity, near-surface optimum ratios, and water retention characteristics in a sandy loam. Dystrophic Paleudalf initially under different tillage methods for sugarcane crop but without tillage for two seasons in southern Brazil. Initial soil tillage systems consisted of no-tillage (NT), compacted no-tillage (NTC), conventional tillage, and chiseling of no-tillage (Ch). Disturbed and undisturbed soil was sampled from 0 to 10, 10 to 20, 20 to 40, and 40 to 60 cm layers to determine degree of compaction, air capacity, near-surface optimum ratios, soil water retention characteristics, and soil physical quality index S. Initially, NT treatment had the significantly ( p &lt; 0.05) lowest degree of compaction (87%), highest soil air capacity (0.104 cm3 cm−3), air capacity/total porosity ratio (0.261), and better water retention characteristics in the surface layer. Over time, Ch had improved the structure of the subsurface soil layers with the lowest degree of compaction (≈88%) and highest air capacity (≈0.140 cm3 cm−3), while the measured indices were poor in NTC. Irrespective of tillage, the surface layer showed resilience during the years without soil disturbance with low degree of compaction, increased water retention, and air capacity. NT could be a good soil management option for sugarcane production, while mechanical chiseling is advocated for ameliorating compacted soils.

Impact of remolded soil fabric on the volumetric deformation behavior of clayey soils

The volumetric deformation of clayey soils, leading to a reduction in the bearing capacity and serviceability of pavements and building structures, is a major concern during their design, construction, and maintenance. Several approaches are often followed to mitigate the volume expansion and concomitant damage, including removal and replacement, moisture treatment with appropriate compaction protocols, and chemical treatment. During these treatment processes, the in-situ fabric is altered as the natural undisturbed soils are remolded and compacted. Hence, it is crucial to understand the effect of remolding on the volumetric characteristics of clayey soils. To investigate this effect, coefficient of linear extensibility (COLE) tests were conducted on both natural and remolded soil samples. The objective was to evaluate the impact of soil fabric modification on volumetric characteristics such as suction compressibility index (γh) and soil water-retention characteristics, i.e., the soil–water characteristic curve (SWCC) of clayey soils. Our findings indicated that remolded soils had approximately 10% to 30 % higher γh-values than those of unaltered soils, which can be attributed to changes in porosity. Two distinct mechanistic models were developed using the packing theory concept to link the γh-value and SWCC of remolded and natural soils. Finally, an analysis was conducted to compare the potential vertical movement (PVM) of natural and remolded clay soils. This analysis revealed that the remolded soil fabric substantially increased the PVM values, particularly for high-plasticity clay soils. This effect should be considered when assessing the impact of treatment that requires remolding, which substantially alters the soil structure and fabric.

A Review on Soils Treated with Biopolymers Based on Unsaturated Soil Theory.

Adding different materials to soil can improve its engineering properties, but traditional materials such as cement, lime, fly ash, etc., have caused pollution to the environment. Recently, biopolymers have shown many advantages, such as economy and environmental protection, which make them applicable to geotechnical engineering. This study summarizes the effects of biopolymers on soil's engineering properties and the main directions of current research. Firstly, the advantages and disadvantages of a variety of widely used biopolymer materials and their effects on the specific engineering characteristics of soil (i.e., water retention characteristics, strength characteristics, permeability characteristics, microstructure) are introduced, as well as the source, viscosity, pH, and cost of these biopolymers. Then, based on the theory of unsaturated soil, the current research progress on the water retention characteristics of improved soil is summarized. The key factors affecting the strength of biopolymer-treated soil are introduced. Due to the actual environmental conditions, such as rainfall, the permeability and durability of biopolymer-treated soil are also worthy of attention. In summary, it is necessary to study the variation laws of the engineering properties of biopolymer-treated soil in the full suction range, and to predict such laws reasonably. The relevant results are conducive to the safer and more scientific application of biopolymers in geotechnical engineering practice.

Vertical Changes of Organic Carbon Storage, Structural and Hydraulic Properties of a Humid Tropical Soil under Vegetation Cover in Southeast Nigeria

Changes in organic carbon storage (OCS), structural and hydraulic properties with depth of soil are important phenomena that need to be understood per soil for effective soil management in crop production. A study was conducted in a Randomized Complete Block Design (RCBD) to assess the vertical changes in OCS, structural and hydraulic properties of soil under vegetation cover. The study involved 5 levels of sampling depth replicated nine (9) times to give forty five (45) observational units. The nine points for sampling were randomly selected and the five sampling depths were 0 – 20, 20 – 40, 40 – 60, 60 – 80 and 80 – 100 cm. Auger and core soil samples were collected at the designated depths. The samples were processed and analysed in the laboratory. The results of data analyses showed that depths varied significantly (P≤0.05) in the parameters measured. The highest OCS of 38.28 ton / ha was obtained at the 0 – 20 cm depth and the lowest (10.38 ton / ha) was obtained at 80 – 100 cm depth. The highest mean weight diameter of 1.00 mm and lowest of 0.72 mm were obtained at 0 – 20 and 80 – 100 cm depths, respectively. The lowest clay dispersion index of 25.43 % and the highest clay flocculation index of 74.53 % were obtained at 80 – 100 cm depth. Clay content and bulk density (BD) increased with depth and, the highest clay content of 212.4 g / kg and highest BD of 1.65 mg / m3 were obtained at the 80 – 100 cm depth. Saturated hydraulic conductivity (Ksat), total porosity (Pt) and macroporosity (Pm) decreased with depth. The highest Ksat of 3.11 cm / hr, Pt of 47.41 % and Pm of 24.13 % were obtained at 0 – 20 cm depth. The water retention characteristics increased with depth. The highest field capacity of 24.46 %, permanent wilting point of 11.16 % and available water capacity of 13.32 % were obtained at 80 – 100 cm depth. There were consistent changes in the soil properties across the depths. The reduced drainage at the higher depths should be considered in cultivating deep rooted crops which are sensitive to poor drainage. The top soils should be managed with good agronomic practices and minimum tillage to avert damages to the microaggregates.

Investigation of Amended Sandy Soil's Water Retention Characteristics

Purpose: Soil water retention studies are important for evaluating soil quality and water movement in soils. This study assesses the water content capacity and changes in the retention curve of sandy soil after using zeolite X, activated charcoal, and rice husk ash as amendments. Design/Methodology/Approach: Sandy soil samples were collected from the study area, Keta District of Ghana, and a core borer and a pot experiment were used to determine the pressure head and water content of the soil. One-way analysis of variance (One-way ANOVA) was used to analyze the data. Water retention curve analyses were conducted using COMSOL multi-physics simulation and experimental work. Research Limitation/Implication: The amendments used were restricted to zeolite X, activated charcoal, and rice husk ash. Practical Implications: The study contributes to the field of sustainable agriculture and land management by exploring amendments that can potentially reduce water usage in irrigation, mitigate soil erosion, and enhance soil fertility. This aligns with global efforts to promote sustainable and efficient resource use. Social Implications: These findings can be implemented for cases of poor yield by farming communities by applying zeolite X, activated charcoal, and rice husk ash to improve crop productivity.

Porous Mineral Amendments Enhance Nitrogen Mineralization via Improvement of Soil Aeration and Water Retention Characteristics

Porous Mineral Amendments Enhance Nitrogen Mineralization via Improvement of Soil Aeration and Water Retention Characteristics

Effects of fir-wood biochar on CH4 oxidation rates and methanotrophs in landfill cover soils packed at three different proctor compaction levels

Application of biochar to landfill cover soils can purportedly improve methane (CH4) oxidation rates, but understanding the combined effects of soil texture, compaction, and biochar on the activity and composition of the methanotrophs is limited. The amendment of wood biochar on two differently textured landfill cover soils at three compaction levels of the Proctor density was explored by analyzing changes in soil physical properties relevant to methane oxidation, the effects on CH4 oxidation rates, and the composition of the methanotrophic community. Loose soils with and without biochar were pre-incubated to equally elevate the CH4 oxidation rates. Hereafter, soils were compacted and re-incubated. Methane oxidation rates, gas diffusivity, water retention characteristics, and pore size distribution were analyzed on the compacted soils. The relative abundance of methanotrophic bacteria (MOB) was determined at the end of both the pre-incubation and incubation tests of the packed samples. Biochar significantly increased porosity at all compaction levels, enhancing diffusion coefficients. Also, a re-distribution in pore sizes was observed. Increased gas diffusivity from low compaction and amendment of biochar, though, did not reflect higher methane oxidation rates due to high diffusive oxygen fluxes over the limited height of the compacted soil specimens. All soils, with and without biochar, were strongly dominated by Type II methanotrophs. In the sandy soil, biochar amendment strongly increased MOB abundance, which could be attributed to a corresponding increase in the relative abundance of Methylocystis species, while no such response was observed in the clayey soil. Compaction did not change the community composition in either soil. Fir-wood biochar addition to landfill cover soils may not always enhance methanotrophic activity and hence reduce fugitive methane emissions, with the effect being soil-specific. However, especially in finer and more compacted soils, biochar amendment can maintain soil diffusivity above a critical level, preventing the collapse of methanotrophy.