Water Ionic Strength Research Articles

Environmental behaviour of iron and steel slags in coastal settings

Iron and steel slags have a long history of both disposal and beneficial use in the coastal zone. Despite the large volumes of slag deposited, comprehensive assessments of potential risks associated with metal(loid) leaching from iron and steel by-products are rare for coastal systems. This study provides a national-scale overview of the 14 known slag deposits in the coastal environment of Great Britain (those within 100 m of the mean high-water mark), comprising geochemical characterisation and leaching test data (using both low and high ionic strength waters) to assess potential leaching risks. The seaward facing length of slag deposits totalled at least 76 km, and are predominantly composed of blast furnace (iron-making) slags from the early to mid-20th Century. Some of these form tidal barriers and formal coastal defence structures, but larger deposits are associated with historical coastal disposal in many former areas of iron and steel production, notably the Cumbrian coast of England. Slag deposits are dominated by melilite phases (e.g. gehlenite), with evidence of secondary mineral formation (e.g. gypsum, calcite) indicative of weathering. Leaching tests typically show lower element (e.g. Ba, V, Cr, Fe) release under seawater leaching scenarios compared to deionised water, largely ascribable to the pH buffering provided by the former. Only Mn and Mo showed elevated leaching concentrations in seawater treatments, though at modest levels (<3 mg/L and 0.01 mg/L, respectively). No significant leaching of potentially ecotoxic elements such as Cr and V (mean leachate concentrations <0.006 mg/L for both) were apparent in seawater, which micro-X-Ray Absorption Near Edge Structure (μXANES) analysis show are both present in slags in low valence (and low toxicity) forms. Although there may be physical hazards posed by extensive erosion of deposits in high-energy coastlines, the data suggest seawater leaching of coastal iron and steel slags in the UK is likely to pose minimal environmental risk.

The Impact of Dissolved Salts on the Erosion of Physically Purified Compacted Bentonite

Compressed bentonite is an appropriate cushion material for getting rid of spent nuclear waste below the surface storage. The contact of compressed bentonite with water, its immersion in water and subsequently its swelling into the neighboring gaps are the principal processes for the development of colloid fines. In this study, to examine the impact of ionic strength on the erosion of compacted bentonite, only the ionic power values of the water contacting the bentonite were changed by keeping the flow rates constant. Sodium chloride was used to change the ionic power of the water contacting the bentonite throughout the experiment. Turbidity meter (TM) was used to determine the amount of eroded particles. The dissolution of bentonite fragments is critical not only due to their physical stability but also due to the radioactive isotopes they contain. An experimentation methodology is introduced. The implemented flow rates were 0.06 ml/min and 0.22 ml/min, and the selected waters were distilled water and 0.05 M NaCl ionic strength water. The dry densities of the compressed bentonite samples used were 1.67 g/cm3, 1.72 g/cm3, 1.72 g/cm3 and 1.72 g/cm3. With distilled water, the total erosion value measured at the end of the 24th day using a flow rate of 0.06 ml/min was 1002.7 mg, whereas the final erosion value measured at the end of the 24th day using water containing 0.05 M NaCl salt was 31.13 mg. In the case of distilled water, the total erosion value measured at the end of the 24th day using a flow rate of 0.22 ml/min was 1774.85 mg, while the total erosion value measured at the end of the 24th day using water containing 0.05 M NaCl salt was 73.6 mg. This demonstrates that the influence of the ionic strongness of the water on erosion is too important to be neglected.

Effect of physicochemical and microbiological factors on the development of viable but non-culturable and resuscitation states of Vibrio cholerae.

Vibrio cholerae can endure harsh environmental conditions by transitioning into viable but non-culturable (VBNC) form and resuscitate upon return of appropriate conditions. In this study, we assessed the impact of physicochemical and microbiological factors, on the development of low temperature-induced VBNC state and subsequent recovery by temperature upshift. In estuarine water, Vibrio cholerae exhibits a slower decline in culturability over a period of 77 days as compared to 10 days in fresh water. When variable cell numbers from different growth phases were used for VBNC induction, it was observed that the higher inoculum size (106-107 cfu ml-1) from the late log phase culture appears to be crucial for entering the VBNC state. Conversely, starved cells could enter the VBNC state with an initial inoculum of 104-105 cfu ml-1, followed by resuscitation as well. The addition of glucose, GlcNAc and mannitol differentially affects progression into VBNC, while the addition of tryptone, yeast extract and casamino acid facilitated early entry into the VBNC state and shortened the length of the recovery period. Altogether these findings demonstrated that the ionic strength of water, inoculum size and the availability of nutrients played distinct roles during VBNC induction and resuscitation.

Side‐Chain Chemistry Governs Hierarchical Order of Charge‐Complementary β‐sheet Peptide Coassemblies

AbstractSelf‐assembly of proteinaceous biomolecules into functional materials with ordered structures that span length scales is common in nature yet remains a challenge with designer peptides under ambient conditions. This report demonstrates how charged side‐chain chemistry affects the hierarchical co‐assembly of a family of charge‐complementary β‐sheet‐forming peptide pairs known as CATCH(X+/Y−) at physiologic pH and ionic strength in water. In a concentration‐dependent manner, the CATCH(6K+) (Ac‐KQKFKFKFKQK‐Am) and CATCH(6D−) (Ac‐DQDFDFDFDQD‐Am) pair formed either β‐sheet‐rich microspheres or β‐sheet‐rich gels with a micron‐scale plate‐like morphology, which were not observed with other CATCH(X+/Y−) pairs. This hierarchical order was disrupted by replacing D with E, which increased fibril twisting. Replacing K with R, or mutating the N‐ and C‐terminal amino acids in CATCH(6K+) and CATCH(6D−) to Qs, increased observed co‐assembly kinetics, which also disrupted hierarchical order. Due to the ambient assembly conditions, active CATCH(6K+)‐green fluorescent protein fusions could be incorporated into the β‐sheet plates and microspheres formed by the CATCH(6K+/6D−) pair, demonstrating the potential to endow functionality.

Side-Chain Chemistry Governs Hierarchical Order of Charge-Complementary β-sheet Peptide Coassemblies.

Self-assembly of proteinaceous biomolecules into functional materials with ordered structures that span length scales is common in nature yet remains a challenge with designer peptides under ambient conditions. This report demonstrates how charged side-chain chemistry affects the hierarchical co-assembly of a family of charge-complementary β-sheet-forming peptide pairs known as CATCH(X+/Y-) at physiologic pH and ionic strength in water. In a concentration-dependent manner, the CATCH(6K+) (Ac-KQKFKFKFKQK-Am) and CATCH(6D-) (Ac-DQDFDFDFDQD-Am) pair formed either β-sheet-rich microspheres or β-sheet-rich gels with a micron-scale plate-like morphology, which were not observed with other CATCH(X+/Y-) pairs. This hierarchical order was disrupted by replacing D with E, which increased fibril twisting. Replacing K with R, or mutating the N- and C-terminal amino acids in CATCH(6K+) and CATCH(6D-) to Qs, increased observed co-assembly kinetics, which also disrupted hierarchical order. Due to the ambient assembly conditions, active CATCH(6K+)-green fluorescent protein fusions could be incorporated into the β-sheet plates and microspheres formed by the CATCH(6K+/6D-) pair, demonstrating the potential to endow functionality.

The effects of epirubicin-loaded Boc-L-Diphenylalanine peptide nanoparticles on cytotoxicity, genotoxicity, oxidative stress, and apoptosis in non-small cell lung cancer cells.

Peptides, which are important components of the human body, appear in different chemistry applications. Perhaps the most important of these applications is the use of these structures in drug delivery systems due to their biocompatibility properties. Diphenylalanine (FF) peptide-based systems, which are part of the ß-amyloid polypeptide sequence and are known as the smallest dipeptide group, are particularly preferred due to their biocompatible nature, thermal stability, high ionic strength in water in new targeted drug systems. Epirubicin, the epimer of doxorubicin, is utilized in treating lung cancer. The side effects and the applied doses of epirubicin are being tried to be reduced. Therefore, in this study, epirubicin-loaded tert-butyloxycarbonyl protected diphenylalanine (Boc)-FF particles were synthesized and characterized and the effects of these peptides on cytotoxicity, genotoxicity, oxidative stress and apoptosis on non-small cell lung cancer cells (NSCLC) (A549) were evaluated. According to the results of the study, it was determined that epirubicin-loaded Boc-FF dipeptides significantly reduced the viability, oxidative stress, and increased DNA damage and apoptosis in the cells. The study suggests that epirubicin-loaded Boc-FF particles can be used as an alternative drug carrier for NSCLC treatment due to their physiological, chemical, and biological activity.

Experimental study of montmorillonite erosion in low ionic strength water under stagnant and flow conditions in artificial fractures

Compacted bentonite has been proposed as a buffer material in several geological repository concepts for high-level radioactive waste. However, in dilute groundwater, bentonite, especially when dominated by Na-montmorillonite, has been found to be susceptible to erosion and excessive mass loss may compromise the safety of the repository. Herein, results from twelve erosion tests with either flowing or stagnant aqueous solutions, performed with custom-designed artificial fractures made of Poly(methyl methacrylate) are reported. The fracture aperture was 0.1 mm in all tests. Na-montmorillonite or an equal mix by weight of Na- and Ca-montmorillonite were employed. Eleven of the tests were performed with the fracture being vertical, and in one test the fracture was horizontal. The transparent artificial fracture setup allowed monitoring of the expansion of the montmorillonite into the fracture. In all but one test, the diameter reached a limit value, normally within one month of the start of the test. The final diameters varied between 5.9 and 8.7 cm and no clear correlation to flow rate, type of montmorillonite or ionic strength could be discerned. The initial diameter of the emplaced clay was 3.5 cm. The erosion was monitored regularly by turbidity measurements. In general, erosion rates decreased with time and were ultimately low enough that an extrapolation or upscaling of the results to repository scale and operating time would imply a total erosion below the critical value for repository safety. This was the case for all vertical fractures, regardless of montmorillonite type and ionic strength of the aqueous solution. The observed decrease in erosion rates was not due to depletion of montmorillonite at the source because at the end of each test 90% or more of the montmorillonite remained. Further, the ionic strengths were below the critical coagulation concentration, hence gelation cannot provide an explanation to the reduction in erosion rates. Finally, the diameters of the extruded montmorillonite reached the limit while the erosion rates continued to decrease. Consequently, the observed limited expansion was not due to a steady state between erosion and swelling. Rather, the expansion behaviour suggests the presence of frictional forces between the montmorillonite and the fracture walls. The results from this study are beyond the current understanding of montmorillonite colloid behaviour and cannot be explained by existing models.

Preliminary investigation on effects of size, polymer type, and surface behaviour on the vertical mobility of microplastics in a porous media

Due to the ubiquitous nature of microplastic (MP), knowledge of its fate and migration in subsurface environments like soil becomes extremely important to understand underlying ecological risk. The fate and migration of MP in subterranean settings like sand are governed by the retention/transport properties influenced by the interaction of sand and MPs. In this study, sand column experiments under simulated rainfall conditions were conducted for 180 days to assess the vertical migration of mixed MPs consisting of polypropylene (PP), polyethylene (PE), and polyethylene terephthalate (PET). Sand column experiments were subjected to 60 wet-dry cycles over 180 days. The effects of polymer type, microplastic size, sand particle size, and surface roughness on the migration of MPs were evaluated. Results showed that the smallest-sized fragmented PE particles had the highest migration potential compared to PET and PE. The ratios of the diameters of MP particles and sand particles (dMP/dsand) played a significant role in determining the penetration depth of the various sizes of MPs. The MP particles with dMP/dsand 0.11 showed greater penetration depth in sand media and were detected in the column effluent water after 60 days of a column run. In addition, surface roughness, low ionic strength water, irregularly shaped particles, and wet and dry cycles contributed to the migration of MPs in the sand column. Three new absorbance peaks corresponding to the hydroxyl, CO stretch, and carbonyl groups evolved in the extracted PE MPs sample from different depths, as shown by FTIR analysis, suggesting that PE MPs had been oxidised. XPS analysis revealed changes in the surface properties of the MPs, indicating that oxidation occurred at the top layer, causing structural deterioration of the PE MPs. However, oxidation of the surface bonds was restricted in the layers underneath due to a lack of oxygen. The finding of the study suggests that in a natural environment, such accumulation and migration of MPs in sandy soil can increase the possibilities to the underlying groundwater contamination.

A Novel Approach to Developing Thresholds for Total Dissolved Solids Using Standardized and Experimental Toxicity Test Methods.

The increasing salinization of freshwater streams from anthropogenic land uses and activities is a growing global environmental problem. Increases in individual ions (such as sodium or chloride) and combined measures such as total dissolved solids (TDS) threaten drinking water supplies, agricultural and economic interests, and the ecological health of freshwater streams. Because the toxicity of high ionic strength waters depends on the specific ion composition, few water quality standards exist to protect freshwater streams from salinization. In the present study, we used a novel approach to develop site-specific and ecologically relevant TDS thresholds for the protection of aquatic life. The first step of the approach was to characterize the ion composition of the waterbody or region of interest and prepare artificial samples to match that composition. Using a combination of standardized toxicity test species and more ecologically relevant field-collected species, toxicity tests were then conducted on these artificial samples prepared at a range of TDS concentrations. The advantage of this approach is that water quality criteria can be developed for easy-to-measure generalized parameters such as TDS while ensuring that the criteria are protective of instream aquatic life and account for the complex interactions of the various ions contributing to salinization. We tested this approach in Sand Branch, Loudoun County, Virginia, USA, where salinization from hard rock mining and urban runoff has impaired aquatic life. Acute and chronic TDS thresholds of 938 and 463 mg/L, respectively, were developed in this stream and used for total maximum daily load development in the watershed. The approach provides a potential model for establishing protective thresholds for other waterbodies impacted by salinization. Environ Toxicol Chem 2022;41:2782-2796. © 2022 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

Avoiding the point of no return: Maintaining infiltration to remediate saline-sodic Vertosols in high rainfall environments

Saline-sodic soils are often too saline and alkaline for plant survival. These soils are prone to dispersing and eroding after high rainfall events when salinity is reduced before the sodicity. Cost-effective and water efficient methods are needed to leach salts while maintaining sufficient ionic strength of the soil solution. We tested the ability of gypsum, both alone and combined with elemental sulfur and organic matter to remediate the upper 15 cm of a strongly saline-sodic alkaline Vertosol when leached with deionised water in repacked columns. Prior to leaching, all amendment combinations reduced soil alkalinity by 80% and dispersion by 47% by displacing exchangeable sodium (Na). After leaching with 600 mm of deionised water, electrical conductivity of the soil solution (ECss) decreased from an average of 38–4.8 dS m−1 at 8 cm depth. Importantly, structure was maintained in all amended soils, despite this decrease in ECss. In contrast, for the control treatment, there was a concomitant loss of soil structural stability as ECss decreased. This decrease in stability also occurred in the subsoils of all treatments (which were not amended) because the applied calcium (Ca) precipitated before it could be leached to remediate the deeper layers. This study demonstrated that it was critical to first apply amendments as deep in the soil profile as possible to prevent the development of a non-saline sodic soil. Leaching the soil with low ionic strength water removed excess soluble salts from the plant root zone. We estimated that > 300 mm of water (rainfall or irrigation) was required to leach through the root zone to ensure a suitable soil profile for establishing of salt tolerant pioneer species such as Rhodes grass (Chloris gayana Kunth).

Attachment, re-mobilization, and inactivation of bacteriophage MS2 during bank filtration following simulation of a high virus load and an extreme rain event

Viruses, including human pathogenic viruses, can persist in water. For producing drinking water from surface water via bank filtration, natural attenuation capacities and the fate of viruses during the passage of aquatic sediments are of particular interest. Moreover, the increasing frequency of extreme hydrological events necessitate re-evaluation of the sustainability and efficacy of processes removing viruses. For this purpose, we performed bank sediment filtration experiments using a mesocosm in a technical-scale experimental facility that simulates a field situation under more tightly controlled conditions. We used the bacteriophage MS2 as a surrogate for enteric viruses to study the transport of different viral loads through the bank sediment. Additionally, we simulated a heavy rain event to investigate the re-mobilization of initially attached virus particles. We quantified the abundance of infectious MS2 phages by plaque assay and the total number of MS2 particles by qPCR. Also, we differentiated pore water concentrations by depths of the sediment column and investigated attachment to the sediment matrix at the end of the individual experimental phases. Bank filtration over a vertical distance of 80 cm through sandy sediment revealed a virus removal efficiency of 0.8 log10 for total MS2 particles and 1.7 log10 for infectious MS2 particles, with an initial phage concentration of 1.84 × 108 gene copies mL−1. A low load of infectious MS2 (1.9 × 106 plaque forming units mL−1) resulted in a greater removal efficiency (3.0 log10). The proportion of infectious MS2 phages of the total MS2 particle mass steadily decreased over time, i.e., in the course of individual breakthrough curves and with sediment depth. The simulated pulse of rainwater caused a front of low ionic strength water which resulted in pronounced phage remobilization. The high proportion of infectious MS2 among the detached phages indicated that attachment to the sediment matrix may substantially conserve virus infectivity. Therefore, the re-mobilization of previously attached viruses owing to hydrological extremes should be considered in water quality assessment and monitoring schemes.

New insights into modeling disjoining pressure and wettability alteration by engineered water: Surface complexation based rock composition study

Several laboratory studies observed wettability alteration of carbonate rocks towards a more water-wet state through modifying the composition of injected water. The change in carbonate surface charge was reported as the underlying reason behind this wettability alteration and the corresponding incremental oil recovery by engineered water injection (EWI). Although the reported results of carbonate rock sensitivity to injected water ionic strength, pH, salinity, and concentration of potential determining ions (Ca 2+ , Mg 2+ , and SO 4 2− ), there is still an ambiguity on the way carbonates interact with injected water/ions. This is due to the different mineralogical compositions and electrokinetic properties of carbonates. Therefore, this paper provides more insight into the role of major rock-forming carbonate minerals (calcite and dolomite) in engineered water injection at different water compositions by utilizing surface complexation modeling (SCM). The study utilizes a geochemical simulator to highlight the effect of dolomite surface complexation reactions. After determining the total rock surface charge and surface potential, the disjoining pressure was calculated to estimate the water film thickness and consequently, infer about wettability alteration. The results showed that a successful EWI operation creates three regimes: displacement regime, surface reaction regime, and quasi-equilibrium regime. These regimes can be characterized by the proposed surface complexation technique to calculate/optimize the injection pore volumes needed for each regime, determine the location of EWI effect in the reservoir, and predict the time ranges when EWI will reach to the surface in the form of additional oil recovery. Also, it was found that the surface reaction regime is the most important one where main surface reactions and oil recovery occur. Furthermore, the study utilizes SCM for both calcite and dolomite minerals for the first time highlighting their importance in geochemical reactions of EWI when the rock has dolomite in its composition. Existing SCM considers oil-brine and rock-brine interfaces where only calcite mineral is captured in the rock-brine interface. The latter is based on assuming that the rock composition is pure calcite, which is not necessary the case. The findings of this study confirm that seawater is capable of inducing wettability alteration in carbonates under certain favorable conditions due to adsorption/desorption of the potentially determining ions. • This paper provides novel insight into the role of major rock-forming carbonate minerals (calcite and dolomite) in EWI/LSWI at different water compositions by utilizing the surface complexation reactions. • It is found that in a successful EWI operation, three regimes would be created, i.e. displacement regime, surface reaction regime, and equilibrium regime. • These regimes can be characterized by the proposed technique to calculate/optimize the injection pore volumes needed for each zone. • The developed approach can determine the location of EWI effect in the reservoir, and predict the time ranges when EWI would reach to the surface in the form of additional oil recovery. • This study utilizes SCM for both calcite and dolomite minerals for the first time highlighting their importance in geochemical reactions during EWI when the rock has dolomite as a constituent.

A new nanocomposite assembled with metal organic framework and magnetic biochar derived from pomelo peels: A highly efficient adsorbent for ketamine in wastewater

A new nanocomposite, magnetically modified metal-organic framework ZIF-8/biochar (MZBC), was synthesized and used for removal of emerging contaminant ketamine (KET). Here, ZIF-8 was used as a sacrificial template and magnetic biochar was derived from pomelo peels. The influence of different pyrolysis temperatures (400, 500, 600 and 700 ºС) on adsorption performance was investigated in detail. The optimal pyrolysis temperature for the preparation of MZBC was obtained at 600 ºС. The maximum adsorption capacity of MZBC-600 for KET was 32.50 mg·g−1, which was 24.8 times higher than that of a single magnetic biochar (MBC-600). MZBC-600 exhibited a high stable adsorption capacity for KET over a wide pH (3−12) range and even high ionic strength in water. The pseudo-second-order kinetic model and Freundlich isotherm model were more suitable to analyze the adsorption process of MZBC-600 to KET, with R2 of 0.996 and 0.998, respectively. Adsorption kinetics, isotherms, pH effect, ionic effect and series of characterization analysis suggested that the adsorption process was dominated by pore filling, π-π interaction, hydrogen bonding and chelation. The sustainable adsorption capacity (>90% of the initial adsorption capacity) of MZBC-600 was well maintained even after five reuse cycles. In a word, this novel nanocomposite (MZBC-600) contributed a high adsorption efficiency, environment-friendliness and good sustainability.

Effect of saline water ionic strength on phosphorus recovery from synthetic swine wastewater

Declining worldwide phosphate rock reserves has driven a growing interest in exploration of alternative phosphate supplies. This study involved phosphorus recovery from swine wastewater through precipitation of struvite, a valuable slow-release fertiliser. The economic feasibility of this process is highly dependent on the cost of magnesium source. Two different magnesium sources were used for phosphorus recovery: pure magnesium chloride and nanofiltration (NF) saline water retentate. The paper focuses on the impact of ionic strength on phosphorus recovery performance that has not been reported elsewhere. Experimental design with five numerical variables (Mg/P molar ratio, pH, PO43−-P, NH4+-N, and Ca2+ levels) and one categorical variable (type of magnesium source) was used to evaluate the effect of ionic strength on phosphorus removal and struvite purity. The experimental data were analysed using analysis of variance (ANOVA) and principal component analysis (PCA). Results indicated that a magnesium source obtained from NF retentate was as effective as MgCl2 for struvite precipitation. It was also revealed that ionic strength had a more positive effect on struvite purity than on phosphorus removal. Within the range of parameters studied in this research, high ionic strength, high pH and wastewater with high phosphate, high ammonium and low calcium contents were found to be the most favourable conditions for struvite precipitation. Findings from this study will be beneficial to determine the feasibility of using high ionic strength saline water, such as NF seawater retentate, as a magnesium source for phosphorus recovery from wastewater that is rich in ammonium-nitrogen and phosphate.

Erosion dynamics of compacted raw or homoionic MX80 bentonite in a low ionic strength synthetic water under quasi-stagnant flow conditions

Bentonite is planned to be used as a backfill material in deep geological repositories (DGR) notably for those to be established in crystalline rock. Potential erosion processes have to be clearly understood in order to properly assess its long-term barrier function. A likely scenario over the lifetime of a DGR in certain geographical locations is the intrusion of low mineralized glacial melt water through low transmissivity bedrock fractures that could interact with bentonite and favor release of clay colloids. The objective of this work was to determine the dynamic of erosion under a glaciation scenario for Na-exchanged, Na-Ca-exchanged, Ca-exchanged and raw bentonite. The different bentonite types cover different potential materials used for backfill and different stages of cation loading via cation exchange processes upon contact with groundwater of different composition. To this end, erosion experiments were carried out with highly compacted bentonite confined by a porous filter (20 μm) under dynamic conditions simulating the presence of a hydraulically active fracture filled with fault gauge and fracture filling minerals.The <500 μm size fractions of untreated MX80 bentonite and after Na- or Ca-homoionization, were used. Two compacted clay pellets (1.6 ± 0.1 g/cm3) of identical composition were placed in separate compartments of double-side reactors. Post-mortem visual inspection and the observed washout of water soluble MX80 components indicated full equilibration of the bentonite with simulated groundwater taking place during the experiment. The erosion was investigated by circulating a low ionic strength (1.6 10−3 M) water at pH 8.4. Bentonite swelling was confined by stainless steel filter plates.The erosion (clay colloid detachment) via a low mineralized groundwater flow was quantified by determining an average eluted mass loss rate (AMLR). AMLR values integrated over 1466 days (4 years) erosion time for the raw MX80 bentonite, the Na-MX80 and the Na-Ca-MX80 are at 0.019 ± 0.003 kg/y/m2, 0.245 ± 0.007 kg/y/m2 and 0.10 ± 0.02 kg/y/m2, respectively. Only by the sensitive ICP-MS analysis of clay-bound Th and U a tiny detachment of particles from Ca-MX80 could be identified, which shows the highest resistivity against erosion (0.004 ± 0.002 kg/y/m2). The experiments pointed to the swelling pressure as main driving force for colloid generation. A possible inhibiting effect of accessory minerals on erosion can also be deduced from this study. Assuming erosion via a groundwater flow from a single fracture intersecting a deposition hole filled with raw MX80 bentonite at an angle of 90o and with a fracture aperture of up to 200 μm, the present estimations indicated that under the given scenario the mass loss limit of 1200 kg bentonite will not be reached during the first 106 years.

Does the high potassium content in recycled winery wastewater used for irrigation pose risks to soil structural stability?

Sodicity, where a high proportion of cation exchange sites are occupied by monovalent Na+ ions relative to divalent cations Ca2+ and Mg2+, is a widespread and serious problem globally as this can result in soil dispersion, low hydraulic conductivity and poor aeration. It has been increasingly recognized that the presence of potassium (K+) can also be a contributor to soil structural stability problems. The aim of this research was to investigate the long term (>15 years) effects of irrigating treated winery wastewater on soil structural stability, for which K+ comprises a high proportion of total cation concentrations, at a vineyard trial site in the Barossa Valley, Australia. Analysis of the long-term soil dataset was undertaken along with laboratory experiments to determine the current or future impacts of high exchangeable K+ on soil hydraulic conductivity and dispersion. Field results showed build-up of K+ in subsoils and a corresponding decline in Mg2+. This appears due to the higher selectivity and exchange of K+ relative to Na+, and higher K/Na ratio in the wastewater. The potential dispersibility of the subsoils was demonstrated via a high turbidity of soil suspensions and high values of the Emerson Dispersion Index. Declining relative hydraulic conductivity occurred for subsoils leached with winery wastewater followed by low ionic strength water. Flocculation measurements were performed in which aliquots of CaCl2 were added to determine the amount of Ca2+ needed to overcome dispersion. We conclude that the long-term use of winery wastewater irrigation has potential to negatively impact soil structural stability via increasing exchangeable K+. Further field research is required but Ca or Mg based amendments could be used to reduce dispersion risks.

Stability and characterization of mixture of three particle system containing ZnO-CuO nanoparticles and clay

The aim of this study was to understand heteroaggregation of mixture of ZnO and CuO nanoparticles (NPs) with clay, for the first time as per the authors' knowledge. Aggregation studies of mixture of ZnO and CuO nanoparticles with clay was done (ionic strength =5 mM; pH 7; nanoparticles concentration = 0.1 mg/L, 1 mg/L, 10 mg/L; Clay concentration = 1, 10, and 100 mg/L and HA concentration = 0.1,1, and 10 mg/L as total organic carbon). Critical coagulation concentration (CCC) and attachment efficiency values of suspensions with ZnO: CuO ratio = 0.1, 1, 10 were also determined. Aggregation and liquid portions of mixture suspension at equal mass ratio were characterized for size, zeta potential (ZP), metal and ion contents, pH and conductivity. Results indicated that CCC was found to be 120.7 mM for ZnO: CuO ratio 10 and 1144 mM for ZnO: CuO ratio 1. Values of attachment efficiency were obtained to be 0.9 and 0.8 for these two ZnO:CuO ratio. At natural water ionic strength (IS) condition, aggregate rate constant of mixture of particles ranged from 0.281 to 8.63 nm/min for 10 mg/L NP concentration. Aggregation in suspension containing mixture of particles was found to be affected by NP concentration, clay concentration, and humic acid (p < 0.05). During a 1-h aggregation study, 2.67 mg Cu metal/g aggregate and 0.38 mg Zn/g aggregate were found in aggregates of 5 mM suspension. Overall, this study provided information on aggregation characterization of mixture of metal oxide nanoparticles (ZnO and CuO) in HA and clay presence, which is useful in understanding aggregation formation and in characterizing exposure dose for environmental risk assessment. More detailed information on a three -particle system with natural colloids is required for predicting their fate in aquatic system and defining risk.

An atomic force microscopy study of calcite dissolution in seawater

We present the first examination of calcite dissolution in seawater using Atomic Force Microscopy (AFM). We quantify step retreat velocity and etch pit density to compare dissolution in seawater to low ionic strength water, and also to compare calcite dissolution under AFM conditions to those conducted in bulk solution experiments (e.g. Subhas et al., 2015, Dong et al., 2018). Bulk dissolution rates and step retreat velocities are slower at high and mid-saturation state (Ω) values and become comparable to low ionic strength water rates at low Ω. The onset of defect-assisted etch pit formation in seawater is at Ω ∼ 0.85 (defined as Ωcritical), higher than in low ionic strength water (Ω ∼ 0.54). There is an abrupt increase in etch pit density (from ∼106 cm−2 to ∼108 cm−2) occurring when Ω falls below 0.7 in seawater, compared to Ω ∼ 0.1 in low ionic strength water, suggesting a transition from defect-assisted dissolution to homogeneous dissolution much closer to equilibrium in seawater. The step retreat velocity (v) does not scale linearly with undersaturation (1-Ω) across an Ω range of 0.4 to 0.9 in seawater, potentially indicating a high order correlation between kink rate and Ω for non-Kossel crystals such as calcite, or surface complexation processes during calcite dissolution in seawater.

Pore-scale study of water salinity effect on thin-film stability for a moving oil droplet

HypothesesThe interfacial dynamics in natural porous media are affected not only by the interplay between viscous and capillary forces but also the solid surface wettability. It has been hypothesized that the wettability alteration induced by changes in the water salinity is primarily caused by electric double-layer force expansion, which strongly affects the multiphase flow dynamics. SimulationsWe investigate the effect of water ionic composition and surface roughness on pore-scale wettability alteration. Multiphase hydrodynamics is numerically captured by a lubrication approximation describing the evolution of thin-films coupled with a multiscale level-set approach. An oil blob mobilized by water within a single pore is considered as a case study. The effect of brine ionic composition is accounted for by an electric double-layer through the water ionic strength and zeta-potential parameters. FindingsWe demonstrate that high-salinity water thin-films collapse to an adsorbed nanometer layer, leading to a large pressure drop during mobilization of the blob induced by the attractive surface forces. However, low-salinity water thin-films are stable due to the repulsive electric double-layer forces, leading to less pressure drop during mobilization of the blob. The novelty of this work lies in efficiently capturing the nanoscale effects of the electric double-layer in pore-scale multiphase flow displacements. Our quantitative investigations provide fundamental insights into the efficiency of low-salinity waterflooding.

Synthesis and application of superabsorbent polymer microspheres for rapid concentration and quantification of microbial pathogens in ambient water

Even though numerous methods have been developed for the detection and quantification of waterborne pathogens, the application of these methods is often hindered by the very low pathogen concentrations in natural waters. Therefore, rapid and efficient sample concentration methods are urgently needed. Here we present a novel method to pre-concentrate microbial pathogens in water using a portable 3D-printed system with super-absorbent polymer (SAP) microspheres, which can effectively reduce the actual volume of water in a collected sample. The SAP microspheres absorb water while excluding bacteria and viruses by size exclusion and charge repulsion. To improve the water absorption capacity of SAP in varying ionic strength waters (0–100 mM), we optimized the formulation of SAP to 180 g⋅L−1 Acrylamide, 75 g⋅L−1 Itaconic Acid and 4.0 g⋅L−1 Bis-Acrylamide for the highest ionic strength water as a function of the extent of cross-linking and the concentration of counter ions. Fluorescence microscopy and double-layer agar plating respectively showed that the 3D-printed system with optimally-designed SAP microspheres could rapidly achieve a 10-fold increase in the concentration of Escherichia coli (E. coli) and bacteriophage MS2 within 20 min with concentration efficiencies of 87% and 96%, respectively. Fold changes between concentrated and original samples from qPCR and RT-qPCR results were found to be respectively 11.34–22.27 for E. coli with original concentrations from 104 to 106 cell·mL−1, and 8.20–13.81 for MS2 with original concentrations from 104 to 106 PFU·mL−1. Furthermore, SAP microspheres can be reused for 20 times without performance loss, significantly decreasing the cost of our concentration system.