The study was performed to identify the imperative pharmacognostic details of berberis aristata DC roots. The root of the plant was standardized by morphologically and microscopically. Further other parameters chemomicroscopical, physical, hytochemical screening and florescence analysis were performed to determine the diagnostic features of berberis aristata DC (B. aristata). Macro and microscopic studies were also reported in the present study. Physicochemical parameters including extractive values, ash values, moisture content have been calculated, which showed 0.79 total ash, 0.05 acid insoluble ash, 20 water soluble ash and 0.90 water insoluble ash. The extractive values of berberis aristata DC were found to be 3.6, 1.2, 0.6 and 2.3 in ethanol, Pet. ether, acetone, and in water respectively. Phytochemical analysis closely revealed the presence of alkaloids in this plant. These outcomes will further help to standardize, identification and in execution research on this plant.