The effects of cold surface temperature, wet air state (temperature and humidity) and original drop size on frost dendrites growth of two neighboring separate frozen water drops of same size under natural convection conditions were investigated by quantitative measurement. It was determined that for different cold plate surface temperature conditions, i.e., the ordinary-low temperature and the cryogenic temperature range, the frost formation mechanism is different. Under the conditions that the air temperature is not too high and absolute humidity is not too excessive, the influence of frozen water drop size on the longest dendrite of frost crystals becomes more and more obvious with the decrease in cold plate temperature. The changes in air temperature and relative humidity both change air absolute humidity, so they have similar effects on the growth of dendrites. However, the effect of wet air state on the growth of frost dendrites is not monotonous, which needs to be considered comprehensively in combination with heat and mass transfer and the existence of heavy phase layer. The thickness of ‘the initial continuous frost layer’ was measured and it was disclosed that the initial frost layer thickness is 1.7–3.0 times that of the height of the frozen water drop diameter. This value may be possibly used as initial frost layer thickness in heat and mass transfer-based frost layer growth prediction models, at least for ordinary-low temperature conditions.