Water-based Formulations Research Articles

Volatilomics Profiling of Counterfeit Perfume by Gas Chromatography Hyphenated to Mass Spectrometry and Fourier-Transformed Infrared Spectroscopy

To prevent deleterious effects on consumers and potential health damage caused by counterfeit perfumes, this study aims to distinguish the original perfume from its suspected counterfeit products. Fingerprint and volatilomics profiling was performed using attenuated total reflection-Fourier infrared spectroscopy (ATR-FTIR) and gas chromatography hyphenated to mass spectrometry (GC-MS). Headspace (HS)-GC-MS was optimized to analyze perfume samples containing water. In the presence of water, our optimized HS-GC-MS method shows linalool's consistent signal intensity, providing an alternative analytical method for water-based perfume formulation. The GC-MS chemical characterization revealed 45 compounds detected in the original sample but only four compounds were detected in the counterfeit products: linalool, citronellol, methyl jasmonate, acetic acid, and propanol. This suggests a clear difference in the formulation of counterfeit products. Counterfeit products also cheat by using a lower amount of ingredients. Relative quantification shows that linalool in counterfeit products was as low as only 5.1% of the amount in the original product. In addition, cheaper and hazardous materials like methanol and 6,7-dihydrogeraniol were detected in counterfeit products. The combination of ATR-FTIR, GC-MS, and HS-GC-MS demonstrated fast authentication of counterfeit perfumes for routine quality control purposes and the possibility of water-based perfume analysis.

A Liquid Well Barrier Element for Temporary Plug and Abandonment Operations: A Breakthrough Approach

Plug and abandonment (P&A) operations demand valuable time and resources for operational procedures and materials to establish the well barrier element. This study aims to investigate the application of a water-based fluid as a liquid well barrier element for temporary abandonment, based on estimates of its lifespan and the survival probabilities of downhole temperatures acquired through accelerated life tests. To achieve this, the water-based formulation was tested and exposed to 95, 110, 140, and 150 °C temperatures for time intervals ranging from 1 to 10 days. After the temperature exposure, the fluid properties were verified, and failure was detected by accounting for any deterioration in rheological parameters and/or a substantial increase in filtrate volume. A statistical analysis of the failure data was performed in RStudio 4.1.3 software using the Weibull Model, and the fluid average lifespans and survival probabilities were estimated for the P&A temperatures. The results obtained demonstrate that the degradation of the fluid was only observed for 140 and 150 °C temperatures. According to the results, the fluid is a promising alternative for temporary abandonment until 80 °C, with no need for monitoring once its lifetime expectation exceeds three years at this temperature. For downhole temperatures above 80 °C, the fluid is a possible alternative, however, the operation’s maximum time and monitoring requirements should consider reliability metrics for each temperature.

Process analytical technology based quality assurance of API concentration and fiber diameter of electrospun amorphous solid dispersions

In this study, a novel quality assurance system was developed utilizing Process analytical technology (PAT) tools and artificial intelligence (AI). Our goal was to monitor the critical quality attributes (CQAs) like drug concentration, morphology and fiber diameter of electrospun amorphous solid dispersion (ASD) formulations with fast at-line techniques. Doxycycline-hyclate (DOX), a tetracycline-type antibiotic was used as a model drug with 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) as the matrix excipient. The water-based formulations were electrospun with high-speed electrospinning (HSES). Raman and NIR sensors and machine vision-based color measurement techniques were employed to accurately determine the drug concentration. Given that morphology can influence the solubility of the drug, a convolutional neural network (CNN)-based AI model was developed to examine this property and detect manufacturing defects. Additionally, the diameter of electrospun fibrous samples was measured using camera images and a trained AI model, enabling rapid analysis of fiber diameter with results similar to that of scanning electron microscopy (SEM). These methods and models demonstrate potential in-line analytical tools, offering rapid, cheap and non-destructive analysis of ASD formulations.

Acetaminophen effects upon formalin-evoked flinching, postformalin, and postincisional allodynia and conditioned place preference.

We explored in mice, the analgesic, tolerance, dependency, and rewarding effects of systemic acetaminophen (APAP). Studies employed adult mice (C57Bl6). (1) Intraplantar formalin flinching + post formalin allodynia. Mice were given intraperitoneal APAP in a DMSO (5%)/Tween 80 (5%) or a water-based formulation before formalin flinching on day 1 and tactile thresholds assessed before and after APAP at day 12. (2) Paw incision. At 24 hours and 8 days after hind paw incision in male mice, effects of intraperitoneal APAP on tactile allodynia were assessed. (3) Repeated delivery. Mice received daily (4 days) analgesic doses of APAP or vehicle and tested upon formalin flinching on day 5. (4) Conditioned place preference. For 3 consecutive days, vehicle was given in the morning in either of 2 chambers and in each afternoon, an analgesic dose of morphine or APAP in the other chamber. On days 5 and 10, animals were allowed to select a "preferred" chamber. Formalin in male mice resulted in biphasic flinching and an enduring postformalin tactile allodynia. Acetaminophen dose dependently decreased phase 2 flinching, and reversed allodynia was observed postflinching. At a comparable APAP dose, female mice showed similarly reduced phase 2 flinching. Incision allodynia was transiently reversed by APAP. Repeated APAP delivery showed no loss of effect after sequential injections or signs of withdrawal. Morphine, but not APAP or vehicle, resulted in robust place preference. APAP decreased flinching and allodynia observed following formalin and paw incision and an absence of tolerance, dependence, or rewarding properties.

Preparation of high-performance water-based ink from acrylic-modified rosin resin

This study focuses on the preparation of a water-based ink binder using acrylic-modified rosin polyethylene glycol ester (ROPEGE). This binder is characterized by a low acid value (12.23 mg NaOH/g), low viscosity (5.40 mPa s), high fixed content (92.40 %), and excellent printability. Compared to reported water-based inks, the inks derived in this study exhibit higher fixed content (resulting in lower VOC emissions), low acid value (indicating high oxidation resistance), and low viscosity (enhancing print quality). The individual effects of emulsifier type, emulsifier content, initiator type, initiator content, acrylic acid content, and dispersant content are analyzed as single factors. The optimal formulation is achieved by selecting specific components: emulsifier PEG-400 (40 wt%/ROPEGE), initiator potassium persulfate (10 wt%/ROPEGE), functional monomer acrylic acid (10 wt% of ROPEGE), and wetting and dispersing agent OT-75 (10 wt%/ROPEGE). The enhancement of the water-based ink formulation is accomplished through the use of acrylic-modified rosin resin (AMRR) as the binder, dimethyl silicone oil as the defoamer, and malachite green as the pigment.

Design of Experiments and Optimization of Monacolin K Green Extraction from Red Yeast Rice by Ultra-High-Performance Liquid Chromatography.

Monacolin K (MK), in red yeast rice (RYR) in the forms of lactone (LMK) and hydroxy acid (AMK), is known for its anti-hypercholesterolemic activity. Under the rising demand for natural bioactive molecules, we present a green ultrasound-assisted extraction (UAE) optimization study for MK in RYR. The development and validation of a fast, sensitive, selective, reproducible, and accurate ultra-high-performance liquid chromatography (UHPLC) method coupled to diode array detection for LMK and AMK allowed us to evaluate the MK recovery in different extract media. Firstly, the ethanol comparability to acetonitrile was assessed (recovery of 80.7 ± 0.1% for ethanol and 85.5 ± 0.2% for acetonitrile). Then, water/ethanol mixtures, with decreasing percentages of organic solvent, were tested by modulating temperature and extraction times. Water extractions at 80 °C for 10 min produced MK yield > 85%. Thus, UAE conditions were optimized by a DOE study using a water-based formulation (mouthwash). The optimal total MK extraction yield (86.6 ± 0.4%) was found under the following conditions: 80 °C, 45 min, 5 mg mL-1 (RYR powder/solvent). Therefore, the new single-process green approach allowed the simultaneous direct extraction of MK and mouthwash enrichment (MK concentration = 130.0 ± 0.6 µg mL-1), which might be tested for the prevention and treatment of periodontitis or oral candidiasis.

Physico-chemical studies and development of water-based drilling fluid formulations with carbon nanotube additives

The paper offers a comprehensive experimental study on the effect of carbon nanotube additives on the features of water-based drilling fluids. A similar study for this fluid has not been carried out before. Nanotubes were used to modify the drilling fluids. Weight concentration of nanomaterials in slurries was from 0.1 to 0.5 %. The formulation of stable drilling slurries modified by carbon nanotube were developed and refined. The study is focused on investigation of rheology, filtration and antifriction properties, temperature and colloidal stability of drilling fluids modified with carbon nanotubes. It is shown that carbon nanotube additives even in small concentrations can significantly change the features of water-based drilling fluids. A comparison was made between the effects of single- and multi-walled carbon nanotubes (SWCNT and MWCNT) on the characteristics of water-based drilling fluids. The study has shown that both types of nano-additives considerably change in the properties of the base fluid. Nevertheless, it was determined that in order to achieve a comparable effect on solution properties, an order of magnitude greater amount of MWCNT additives is required in comparison to single-walled carbon nanotubes.

Towards eco-friendly plant growth regulator: A robustly stable and efficient uniconazole emulsion based on onion-like lamellar liquid crystal

As a commonly water-based pesticide formulation, conventional Emulsions in Water (EWs) always suffer from demulsification in actual complex environments and burst release due to their thermodynamic instability. The ecotoxicity and phytotoxicity caused by chemosynthetic adjuvants cannot be ignored. To address these issues, herein, using a soybean lecithin-based lamellar liquid crystal emulsion (LCE) as the carrier, we construct a robustly stable and low-toxicity EW with the “onion-like” bionic structure and achieve the efficient delivery of plant growth regulator (PGR) uniconazole (UZ). The hydrophobic UZ is firmly encapsulated within the orderly assembled Oil-in-Liquid Crystal-in-Water (O/LC/W) multilayers. This confinement endows UZ-LCE with robust stability, even while encountering disturbances such as dilution, shaking, and changes in pH, ions, and temperature. Furthermore, UZ-LCE exhibits enhanced wettability and affinity to foliar surfaces, as well as specific sustained-release ability. Owing to these superiorities of UZ-LCE, during two consecutive years of efficacy evaluations in cotton field experiments, UZ-LCEs administered through drip irrigation and foliar spray demonstrate remarkable enhancements in controlling excessive plant growth, increasing chlorophyll content, and boosting flower bud formation, surpassing the effectiveness of conventional EWs. UZ-LCE even outperforms commercial preparations (WP and SC) in balancing vegetative growth and reproductive growth, resulting in an 8.3% increase in yield. This research provides an innovative pesticide formulation with eco-friendly adjuvants, less organic solvent, and synergistic functionalities, which is prospective to be extended to other pesticide EWs and contribute to sustainable agriculture.

EVALUATION OF FLAXSEED OIL AND TRICHODERMA SPECIES AGAINST GREY MOLD DISEASE OF GRAPES

Grapes (Vitis vinifera L.) are included in vital crops in the world with respect to its nutritious and economic value. Botrytis grey mold, also called noble rot, harms grapes and wine quality caused by Botrytis cinerea. Other than chemicals Trichoderma species considered as important bio control agents for management of post-harvest diseases. The current study was designed to evaluate Trichoderma species for their Micoparasitic ability using a modification of a pre-colonized plate method. In-vitro efficacy of flaxseed oil against the Botrytis cinerea of grapes were determined using disc diffusion technique with different oil concentrations 0.2%, 0.6%, and 0.8%. In-vivo efficacy of most effective formulated and non-formulated Trichoderma concentrations was determined by applying on grape bunches against Botrytis cinerea. Trichoderma Harzianum is more effective and showed 67.5% growth inhibition than Trichoderma Viridae having 64% growth inhibition while flaxseed oil with 0.2%, o.6% and 0.8% concentrations showed 30.19%, 33.32% and 34.89% growth inhibition against botrytis cinerea respectively. In water-based Trichoderma concentrations (1×10⁸) showed maximum growth inhibition after 6 days that was 76.46%. However, it was observed that the percent disease severity index of the grape fruits in oil-based formulation showed 16 %, 20 %, and 18% after the 9 days of incubation whereas the water-based Trichoderma formulations showed 20%, 22%, 24% respectively as compared with control. Trichoderma species can be considered as bio-control agent while emulsion oil can be used for better shelf life.

Optimization of Water Based Drilling Fluid Properties with the SiO2/g-C3N4 Hybrid.

Drilling fluids are an essential component of drilling operations in the oil and gas industry. Nanotechnology is being used to develop advanced drilling fluid additives. This study looked at the viability of synthesizing SiO2/g-C3N4 hybrid extending the Stober process followed by its addition in different concentrations to water-based drilling fluids and studying impact on the rheological and fluid loss properties of the fluids. The synthesized hybrid was analyzed using XRD, SEM, TGA, and FTIR. Subsequently, it was used to develop the water-based drilling mud formulations and subjected to measurements in accordance with API standard practices. The studies were carried out at various SiO2/g-C3N4 nanoparticle concentrations under before hot rolling (BHR) and after hot rolling (AHR) conditions. The outcomes demonstrated that the rheological and fluid loss properties were enhanced by the addition of SiO2/g-C3N4 nanoparticles, as it worked in synergy with other additives. Additionally, it was discovered that the nanoparticles improved the drilling fluid thermal stability. The experimental findings indicate a significant influence of SiO2/g-C3N4 nanoparticles on base fluid properties including rheology and fluid loss as the most remarkable, especially at higher temperatures. The significant improvements in yield point and 10 s gel strength were 55 and 42.8% under BHR and 216 and 140% under AHR conditions, respectively. Permeability plugging test (PPT) fluid loss was reduced by 69.6 and 87.2% under BHR and AHR conditions, respectively, when 0.5 lb/bbl nanoparticles were used in formulations. As a result, SiO2/g-C3N4 nanomaterial has the potential to be used as drilling fluid additive in water-based drilling fluids.

3D Printing for catalytic activity – Ink development using a drop-on-demand cartridge

Powder-bed binder-jetting 3D printing requires the use of a liquid binder or ink. These binders/inks have been widely tailored for individual application. This article covers the proof-of-concept development of an ink for a powder-bed binder-jetting 3D printer. The ink formulation in our work is both catalytically active and is used to bind the catalyst substrate. A modified, commercially available inkjet cartridge is used to spread liquid ink onto a powder surface, forming shapes in a layer-wise fashion to produce green bodies that are heat-treated post-print. The important parameters for manipulating rheology for inkjet printing are explored as a function of ink formulation. Specifically, the relationship between density, viscosity, and surface tension dictates the suitability for printing and those properties can be controlled by using differing amounts of the ink’s constituent components. Relationships correlating density to solvent concentration and viscosity to binder and dispersant concentrations are found, which are used to determine a water-based ink formulation suitable for use in the commercially available ink cartridge. The results of an initial print are presented and show excellent dispersion of the nickel catalyst.

Investigation of TiO2 replacement alternatives for water-based paints using styrene butyl acrylate

Purpose Titanium dioxide (TiO2) has high opacity, high brightness and whiteness, owing to its high refractive index value. It is mainly used in the coating industry and continuous efforts have been made to replace some of the TiO2 in paint with new pigments. This study aims to replace part of TiO2 pigment with various percentages of BaSO4, CaCO3 and kaolin in styrene butyl acrylate-based paint formulations, without changing the properties of paints using only titanium dioxide. Design/methodology/approach To determine the optimum use rate of new pigment mixing, opacity, gloss, scrub resistance and weather resistance properties have been investigated in the water-based paint formulation. The morphological properties of these samples were examined by scanning electron microscopy analysis. Findings In the total color change (ΔE) measurements, it was observed that the sample coded 85Ti/15Ba produced extremely similar results to the situation when TiO2 was used alone. It was seen that the best results were obtained when 85Ti/15Ba was used instead of TiO2. Originality/value Comparison research on the impact of replacing TiO2 with BaSO4, CaCO3 and kaolin on the performance characteristics of water-based styrene butyl acrylate-based paint formulations has not been done in the literature, according to the literature search.

Gum Arabic-stabilized upconverting nanoparticles for printing applications

Upconverting nanoparticles (UCNPs) have been proposed for a variety of applications ranging from biomedical probes to luminescent sensors and security tags. Yet, bringing UCNPs into real-life, technologically relevant products requires implementation into industry-friendly processes. The need for stable dispersions, clean films or dry powders challenges users who look for a way to use UCNPs. In this work, an ink formulation was developed that offers a straightforward way to print UCNPs on glass and metallic substrates. The use of Gum Arabic as biocompatible emulsifier allowed to implement the NaGdF4:Er,Yb/NaGdF4 core/shell UCNPs into water-based ink formulations without the need of complex surface chemistry. The formulation, based on water, glycerin, and propanediol, exhibited good stability and applicability for printing with a commercial aerosol jet printer. Bright upconversion emission was retained upon printing, and the obtained UCNP films were used in proof-of-concept luminescent thermal sensing.

Rheological Study of Salted Water-Based Drilling Fluid Formulations Developed Using a Novel Amphoteric Terpolymer

Rheological Study of Salted Water-Based Drilling Fluid Formulations Developed Using a Novel Amphoteric Terpolymer

Smart controlled-release nanopesticides based on metal-organic frameworks.

The practical utilization rates of conventional pesticide formulations by target organisms are very low, which results in the pollution of ecological environments and the formation of pesticide residues in agricultural products. Water-based nanopesticide formulations could become alternative and effective formulations to eventually resolve the main issues of conventional pesticide formulations. In this feature article, we describe the design concept of smart (stimuli-responsive) controlled-release nanopesticides, which are created toward hierarchical targets (pests, pathogens, and foliage) in response to multidimensional stimuli from physiological and environmental factors (such as sunlight) of target organisms and plants, for achieving enhanced insecticidal and fungicidal efficacies. The pore sizes and functionalities of metal-organic frameworks (MOFs) can be fine-tuned through the choice of metal-containing units and organic ligands. Tailor-made MOF nanoparticles with large microporous or mesoporous sizes, as well as good biocompatibility and high thermal, mechanical, and chemical durabilities, are used to load pesticides within these pores followed by coating of plant polyphenols and natural polymers for stimuli-responsive controlled pesticide release. This feature article highlights our works on smart controlled-release MOF-based nanopesticides and also includes related works from other laboratories. The future challenges and promising prospects of smart controlled-release MOF-based nanopesticides are also discussed.

Effect of Pink Water Biosolve on Expression of some Cytokines in Hepatocyte of Wistar Rat

The use of Pink Water Biosolve for clean-up of the environment as a bioremediating chemical has a wide application in the clean-up of crude oil spill in Nigeria. BioSolve is a water-based formulation of nonionic surfactants and other specialty chemicals. The level of toxicity resulting from environmental exposure of plants and animals, including humans, to this chemical has not been well understood. Thus, the level of expression of pro-inflammatory and anti-inflammatory cytokines in liver cells of Wistar rats exposed to drinking water obtained from cleaned-up environment was investigated in this study. Reverse transcriptase PCR technique was used to assess the expression of selected hepatic cytokines. The results showed that 10% Soluble Crude Oil Portion (SCP) caused an increased expression of the Interleukin 1-beta (IL1β), Nuclear Factor kappa-B (NF-κB), Interleukin 6 (IL-6), Interleukin 10 (IL-10), Mitogen-activated protein kinase 8 (MAPK8) and Toll-like receptor 4 (TLR4) respectively when compared to the control. The combination of 10% SCP with 0.36mg/L BS and 0.50mg/L BS respectively caused significant (p<0.05) increases in the expression of IL-6, IL-10, MAPK8 and TLR4 respectively. IL-1B was nonsignificantly (p<0.05) increased when compared to the control. This suggests that the presence of the substance in underground/surface water, if found in concentration as low as 0.36mg/L, would be toxic to Wistar rats when consumed over a prolonged period (above 2 weeks).

Citrus waste fibres for natural cosmetic and bioplastic packaging

In this work new biocomposite materials from wasted citrus peels has been validated for a value-added packaging and novel cosmetic products. Specifically, extracted natural fibres and dehydrated pulp from citrus fruit were combined with polylactic acid and other additives as materials for the production of bio-based packaging jars and cosmetic products. For the composition of the packaging an orange powder (dehydrated wastes) was found to be the best performing, while orange comminute, which results from milling the orange peel, was most suitable for composition of the cosmetic formulations. Polylactic acid was compounded with citrus fibres by up to 25 wt.% by twin screw extrusion and processed via injection moulding, one of the most widespread processing technologies for producing rigid packaging containers. Composites were characterized, defining their mechanical, morphological and thermal properties. Validation of packaging thermostability performance was performed by Heat deflection temperature in compliance to ISO75 and VICAT softening temperature in accordance with ISO306. Compatibility tests of packaging demonstrators with newly formulated cosmetic products has been investigated as well. An accelerated compatibility test of the cosmetic jars with water and oil-based simulants was performed at ambient temperature, -5 °C, and at 45 °C. The results indicated that new cosmetic packaging are not consistent with water-based cosmetic formulations but are compatible with products based on natural oils. While the price of these products is generally higher than those using conventional plastics, they are competitive for premium cosmetic brands.

Tailoring Water-Based Graphite Conductive Ink Formulation for Enzyme Stencil-Printing: Experimental Design to Enhance Wearable Biosensor Performance

Tailoring Water-Based Graphite Conductive Ink Formulation for Enzyme Stencil-Printing: Experimental Design to Enhance Wearable Biosensor Performance

Chromatographic separation of silver-111 from neutron-irradiated palladium target: toward direct labeling of radiotracers

BackgroundSilver-111 is a promising β−-emitting radioisotope with ideal characteristics for targeted radionuclide therapy and associated single photon emission tomography imaging. Its decay properties closely resemble the clinically established lutetium-177, making it an attractive candidate for therapeutic applications. In addition, the clinical value of silver-111 is further enhanced by the existence of the positron-emitting counterpart silver-103, thus imparting a truly theranostic potential to this element. A so-fitting matching pair could potentially overcome the current limitations associated with the forced use of chemically different isotopes as imaging surrogates of lutetium-177, leading to more accurate and efficient diagnosis and treatment. However, the use of silver-111-based radiopharmaceuticals in vivo has faced obstacles due to the challenges related to its production and radiochemical separation from the target material. To address these issues, this study aims to implement a chromatographic separation methodology for the purification of reactor-produced silver-111. The ultimate goal is to achieve a ready-to-use formulation for the direct radiolabeling of tumour-seeking biomolecules.ResultsA two-step sequence chromatographic process was validated for cold Ag-Pd separation and then translated to the radioactive counterpart. Silver-111 was produced via the 110Pd(n,γ)111Pd nuclear reaction on a natural palladium target and the subsequent β−-decay of palladium-111. Silver-111 was chemically separated from the metallic target via the implemented chromatographic process by using commercially available LN and TK200 resins. The effectiveness of the separations was assessed by inductively coupled plasma optical emission spectroscopy and γ-spectrometry, respectively, and the Ag+ retrieval was afforded in pure water. Recovery of silver-111 was > 90% with a radionuclidic purity > 99% and a separation factor of around 4.21·10−4.ConclusionsThe developed separation method was suitable to obtain silver-111 with high molar activity in a ready-to-use water-based formulation that can be directly employed for the labeling of radiotracers. By successfully establishing a robust and efficient production and purification method for silver-111, this research paves the way for its wider application in targeted radionuclide therapy and precision imaging.

Immune profiling and tracking of two-dimensional transition metal dichalcogenides in cells and tissues

Two-dimensional (2D) transition metal dichalcogenides (TMDs), such as molybdenum disulfide (MoS2) and tungsten disulfide (WS2), have attracted considerable interest in biomedicine due to their unique combination of physicochemical properties. The effect of nanomaterials on immune cells and their biodistribution are critical aspects of their clinical translation. However, understanding the interactions of these emerging 2D nanomaterials with the complex pool of peripheral blood mononuclear cells (PBMCs) at the single-cell level and their in situ localization in the main organs still needs to be discovered, preventing their translation in medical settings. Here, we report in-depth immune profiling of water-based and defect-free 2D formulations of MoS2 and WS2 through the simultaneous label-free tracking of their immune cell interactions, both ex vivo in human PBMCs and in vivo in mice by high-dimensional analytical approaches, as well as their biodistribution. For comparison, we studied graphene, the hitherto most explored 2D material for biomedical applications. First, we assessed the impact at the protein and gene level by multiplex protein arrays and RNA sequencing, demonstrating a very modest effect of MoS2 and WS2 on immune cell functionality compared to graphene. Then, a single-cell view of the effects of MoS2 and WS2 on 16 primary human immune cell types in terms of viability and functionality was obtained by single-cell mass cytometry by time-of-flight (CyTOF). We explored over 30 markers looking at multiple cell parameters. Finally, we present evidence that MoS2 and WS2 are visible, without the need for labeling, at the single-cell and tissue level by CyTOF, imaging mass cytometry, and multiplexed ion beam imaging by time-of-flight (MIBI-TOF). In particular, MoS2 and WS2 could be detected in the molybdenum (95Mo) and tungsten (180–186W) channels, respectively, which are not used for commercial mass cytometry tags, allowing for the simultaneous interrogation of a wide variety of biological parameters ex vivo and in vivo following intravenous administration of the TMDs. Indeed, we demonstrated the accumulation of TMDs in the main organs by MIBI-TOF and they could also be identified in specific immune cell subsets by CyTOF. Among the two TMDs studied, WS2 exhibited the highest brightness and signal intensity in all the cell subpopulations and tissues analyzed. In conclusion, we identified TMDs as immune-compatible nanoplatforms, traceable at the single-cell and tissue (sub-organ) levels, thus opening up new perspectives for their exploration in biomedicine.