Different morphologies of nanostructured TiO2 materials can be tailor-made as a function of growth time varied from 1 to 7 h with a step of 2 h. TiO2 nanostructures were prepared on titanium foil by a facile water-assisted chemical vapor deposition method at 850°C. Scanning electron microscopy examination of the samples reveled that, under the treatment time of 7 h, the morphology of nanostructures transformed from cubic nanostructure-like to agglomerated nanorod-like and nanoplate shape. The X-ray diffraction through synthesized TiO2 nanostructures exhibited the crystalline structure of rutile TiO2 formed during the synthesis process. X-ray diffraction analysis showed that at deposition time of 7 h the intensity peak of [110] plane strongly decreased and seems that the [210] plane peak is the preferred orientation at this condition. Present paper deals with studies of the antibacterial activity of different synthesized samples after 24 h in the presence of Escherichia coli and Staphylococcus aureus cells. It is found that the growth time has an essential role in the morphology and antibacterial properties of TiO2 nanostructures.