New Disease ReportsVolume 29, Issue 1 p. 11-11 ArticleOpen Access First report of Lasiodiplodia theobromae causing canker on tapped Boswellia papyrifera trees in Ethiopia Alemu Gezahgne, Corresponding Author Alemu Gezahgne alexshig@yahoo.com Forestry Research Center, P.O. Box 30708, Addis Ababa, EthiopiaSearch for more papers by this authorAbraham Yirgu, Abraham Yirgu Forestry Research Center, P.O. Box 30708, Addis Ababa, EthiopiaSearch for more papers by this authorHabtemaria Kassa, Habtemaria Kassa Center for International Forestry Research (CIFOR), P.O. Box 5689, Addis Ababa, EthiopiaSearch for more papers by this author Alemu Gezahgne, Corresponding Author Alemu Gezahgne alexshig@yahoo.com Forestry Research Center, P.O. Box 30708, Addis Ababa, EthiopiaSearch for more papers by this authorAbraham Yirgu, Abraham Yirgu Forestry Research Center, P.O. Box 30708, Addis Ababa, EthiopiaSearch for more papers by this authorHabtemaria Kassa, Habtemaria Kassa Center for International Forestry Research (CIFOR), P.O. Box 5689, Addis Ababa, EthiopiaSearch for more papers by this author First published: 24 March 2014 https://doi.org/10.5197/j.2044-0588.2014.029.011Citations: 1AboutSectionsPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Boswellia papyrifera (Burseraceae) is found mainly in the Amhara, Tigray and Benshangul Gumze Regions of Ethiopia (Vollesen, 6). This economically important tree species is widely used for frankincense (incense) production. Incense is extracted by frequent, intensive and repeated wounding made at different directions and positions on the bole of the tree. Depending on the size of the tree, there could be between 6 and 16 tapping spots that are refreshed and widened 8-12 times each year at an interval of 15 to 20 days (Gebrehiwot, 1). Tapping for incense has a negative impact on the survival rate, growth and reproduction of the tree (Rijkers et al., 5) and wounding predisposes trees to microbial infection. Disease symptoms and death of Boswellia trees have been commonly observed in all areas where tapping has been practiced. Specific symptoms include canker formation, exudation of gum, wilting, dieback, vascular browning and death of the tree. This study was carried out to determine the cause of the observed disease on tapped Boswellia trees. Samples were collected from Humera and Metema Districts, North Ethiopia. Isolation was made from ten tapped trees that developed scars and galls on the stem (Figs. 1, 2). No clear disease symptoms were observed in untapped trees. Pieces of wood pieces (5 mm2) were taken from each sampled tree, surface sterilised with 70% alcohol and inoculated on to malt extract agar (MEA) amended with streptomycin. Such pieces of wood were then incubated in a moist chamber at 25°C with 12 h light/dark cycle to induce fruiting structures. The pycnidia produced on the wood had a conical shape and were covered by dark grey mycelium. Spores collected from crushed pycnidia were transferred on to MEA. Pieces of mycelia from emerging fungal colonies were transferred to water agar medium containing sterilised pine needles and incubated at 25°C to produce spores for further morphological characterisation. Figure 1Open in figure viewerPowerPoint Figure 2Open in figure viewerPowerPoint Colonies obtained from the different isolation procedures produced white and dense aerial mycelium, later turning black at the top and grey to black on the reverse side of the Petri dish. The brown coloured conidia were single septate, sub-ovoid to ellipsoid and longitudinally striated (Fig. 3) with dimensions of 19-30 times 12.5-15 μm. These morphological characteristics were identical with the description of Lasiodiplodia theobromae (Pat.) Griffon & Maubl (syn. Botryodiplodia theobromae), the anamorph of Botryosphaeria rhodina Berk & Curt. Arx (Punithalingam, 4). Figure 3Open in figure viewerPowerPoint A pathogenicity test was conducted in situ on the branches of ten randomly selected Boswellia trees. Two and three isolates of L. theobromae on MEA from Metema and Humera Districts respectively and pure MEA (control) were used. A 5 mm bark borer was used to make inoculation holes on the bark of B. papyrifera trees. Inoculated branches were covered with Parafilm to hold inoculum in place as well as to avoid desiccation and contamination of the inoculum. After three months, the size of the lesions developed on branches inoculated with L. theobromae measured between 14.3 and 15.7 cm (Fig. 4). No lesion developed on trees treated with the control. This test confirmed that L. theobromae is pathogenic to B. papyrifera trees in Ethiopia. L. theobromae has previously been reported to cause dieback and death of mango and cacao trees in Pakistan and Cameroon respectively (Khanzada et al., 2; Mbenoun et al., 3). This is the first report of L. theobromae infecting Boswellia papyrifera in Ethiopa, which represents a new constraint to the sustainable management of Boswellia papyrifera and incense production. Figure 4Open in figure viewerPowerPoint Acknowledgments The authors would like to thank the Austrian Development Cooperation for financing this study through CIFOR Ethiopia, and the CIFOR for its logistical support. References 1Gebrehiwot K, 2003. Ecology and management of Boswellia papyrifera (Del.) Hochst. Dry forest in Tigray, Northern Ethiopia. Georg-August-University of Göttingen, Germany, PhD thesis. Google Scholar 2Khanzada MA, Lodhi AM, Shahzad S, 2004. Mango dieback and gummosis in Sindh Pakistan caused by Lasiodiplodia theobromae. Plant Health Progress, 1094. http://www.plantmanagementnetwork.org/pup/php/diagnosisguide/2004/mango/Google Scholar 3Mbenoun M, Momo Zeusta EH, Samuels G, Nsouga Amougou F, Nyasse S, 2008. Dieback due to Lasiodiplodia theobromae, a new constraint to cocoa production in Cameroon. Plant Pathology 57, 381. 10.1111/j.1365-3059.2007.01755.xWiley Online LibraryWeb of Science®Google Scholar 4Punithalingam E. 1976. Botryodiplodia theobromae. CMI Description of Pathogenic Fungi and Bacteria, No 519 Kew, Surrey, UK: Commonwealth Mycological Institute. Google Scholar 5Rijkers T, Ogbazghi W, Wessel M, Bongers F, 2006. The effect of tapping for frankincense on sexual reproduction in Boswellia papyrifera. Journal of Applied Ecology 43, 1188– 1195. 10.1111/j.1365-2664.2006.01215.xWiley Online LibraryWeb of Science®Google Scholar 6Vollesen, K, 1989. Burseraceae. In: I Hedberg, S Edwards, eds. Flora of Ethiopia, Volume 3, National Herbarium, Addis Ababa University and Uppsala University, 442– 447. Google Scholar Citing Literature Volume29, Issue1January 2014-June 2014Pages 11-11 FiguresReferencesRelatedInformation