Waters Of Washington Research Articles

Rockfish Species Trends in Puget Sound, Washington, USA, 2009–2023

Rockfishes (Sebastes spp.) are a long-lived, understudied, multi-species fish assemblage in inland Washington (USA) waters. Driven by large-scale fishery removals in the 1970s, their numbers declined and have yet to recover, perhaps due to slow maturation and rare recruitment success. Since then, rockfish fishing restrictions have increased within Puget Sound, culminating in a 2010 total ban. Here, we analyze rockfish community trends (abundance and Shannon Diversity) within Puget Sound from 2009 to 2023. Our dataset includes 157 recorded dive transects from nine rocky or artificial reef habitats at depths 5–24 m throughout four Puget Sound basins: Admiralty Inlet (2 sites), Central Puget Sound (4 sites), Hood Canal (2 sites), and South Puget Sound (1 site). Significant differences in community species composition between basins were observed; we noted more Black (S. melanops), Yellowtail (S. flavidus), and Puget Sound (S. emphaeus) rockfish and fewer Brown rockfish (S. auriculatus) at sites in Admiralty Inlet vs. Central Puget Sound. Adult rockfish counts and Shannon Diversity varied seasonally, with higher rockfish counts and diversity indices in summer/autumn vs. winter/spring. Notably, we observed that total adult rockfish count per survey tended downward over time, driven partly by significant downward trends in Hood Canal Copper rockfish (S. caurinus) counts. We recommend continued monitoring and additional investigation into what factors besides fishing may be driving the trends reported here.

Distribution and Abundance of California (Zalophus californianus) and Steller (Eumetopias jubatus) Sea Lions in the Inshore Waters of Washington, 2013-2016

Two species of sea lions occur in the in387land waters of Washington State: the California sea lion (Zalophus californianus) and the Steller sea lion (Eumetopias jubatus). Both species breed elsewhere, but they typically move into Puget Sound and adjacent waters of the Salish Sea from autumn through spring. There is a need for information on their current abundance and seasonal use patterns as both species prey heavily on threatened/endangered stocks of salmon and steelhead trout (Oncorhynchus spp.), and empirical abundance estimates of these species are lacking for inland Washington waters. From 2013 to 2016, we conducted 39,399 km of aerial surveys for marine mammals in this area, sighting 255 groups of sea lions. We used a subset of 7,841 km of effort and 165 sea lion sightings made during surveys in good sighting conditions to estimate in-water abundance using line-transect methods. Historical tagging data collected in Pacific Northwest waters were used to evaluate the proportions of time that each species spent on land and conducting dives, and then to develop correction factors to derive total abundance for both sea lion species, providing the first empirical abundance estimates for these waters. We estimated that between 33 and 442 California sea lions were found in Puget Sound/Hood Canal in different seasons, with nearly 3,000 being found in the broader inland Washington waters in the peak season (spring). Steller sea lions occurred in much smaller numbers, with a peak of 219 animals in Puget Sound/Hood Canal/Strait of Juan de Fuca in autumn (and possibly as many as 600 to 700 in the entire study area). While some estimates suffer from low precision, this study demonstrates that substantial numbers of sea lions use waters of the study area throughout much of the year. Our results provide an important step toward a better understanding of these two species in the inland waters of Washington, as well as their potential effects on protected salmonid prey species.

Spatio-temporal variation in zooplankton community composition in the southern Salish Sea: Changes during the 2015–2016 Pacific marine heatwave

We examined zooplankton collected across a latitudinal gradient in the southern Salish Sea for changes during the 2015–2016 Pacific marine heatwave (MHW). Zooplankton were collected biweekly in vertical net tows from three regions of Puget Sound and one in northern Washington waters near the Strait of Juan de Fuca, from 2014 to 2020. Water temperatures were cooler in the north and warmer in the south, with anomalously high temperatures occurring in 2015–2016 in all regions. Chlorophyll biomass showed no consistent relationship with temperature. All regions had anomalously high zooplankton biomass in 2015, which continued in the three Puget Sound regions through 2017, with the two central stations showing the most dramatic increases. Several large crustacean species and molluscs showed positive biomass anomalies during the MHW. Euphausiid furcilia showed differential response by species. Puget Sound resident copepod species showed primarily positive anomalies, while some boreal and subarctic copepod species were anomalously low during this period. Gelatinous zooplankton had somewhat higher biomass through the first few years of the study, but patterns showed no clear relationship with the MHW. Differing zooplankton patterns compared to those observed in the California Current, as well as prolonged effects on zooplankton biomass in Puget Sound, suggest that local forcing played a dominant role, likely differing from the mechanisms affecting zooplankton in the California Current. Primary postulated factors include increased growth and metabolism due to higher temperatures, nutrient-driven increases in primary production, and changes in species composition advected from the ocean. Further research is needed to determine the ramifications of the changes and how they may affect the food web in the future.

Seasonal movements and ecological markers as evidence for migration of common minke whales photo-identified in the eastern North Pacific

In the eastern North Pacific Ocean, common minke whales (Balaenoptera acutorostrata) are widespread but encountered relatively infrequently. It is generally believed that they make annual migrations between higher latitudes in the summer and lower latitudes in the winter; however, in some temperate coastal regions where common minke whales have been sighted year-round they have been referred to as resident. To determine movement patterns of common minke whales found in coastal waters of British Columbia and Washington we examined photo-identification data that were collected opportunistically from 2005–12. These data were from four non-overlapping areas between 48°N and 53°N. Despite year-round search efforts, common minke whales were only encountered between April and October. Most of the 44 unique individuals identified in 405 encounters displayed fidelity to areas both within and among years. Five of these whales made relatively large-scale intra-annual movements between areas on six occasions. They were documented to move up to 424km in a northerly direction in spring and up to 398km in a southerly direction in autumn. The seasonal patterns of these movements provide new insights into the foraging ranges and migrations of the individuals. Ecological markers provide evidence that the common minke whales photographed undertake annual long distance migrations. Scars believed to be from cookiecutter shark (Isistius brasiliensis) bites were observed on 43 individuals and the majority of whales documented with good quality images each year had acquired new scars since the previous year. Furthermore, the commensal barnacle Xenobalanus globicipitis was observed on three individuals. Since these sharks and barnacles are from relatively warm waters, it can be inferred that they interacted with the common minke whales at lower latitudes. These findings may have important implications for the definition and management of common minke whale stocks and/or populations in the eastern North Pacific.

Determining effectiveness of dungeness crab escapement in derelict traps

The prevalence and impacts of derelict crab traps in the Washington waters of the Salish Sea have been well documented. When properly equipped with biodegradable escape cord, a derelict trap becomes “disabled” upon escape cord degradation, allowing an egress route for entrapped crab to escape. However, among trap designs commonly used in the region, the effectiveness of escapement (egress) varies. We conducted a laboratory experiment simulating derelict traps to analyze the escapement effectiveness of 13 recreational trap designs, some with modifications. The least successful trap designs in allowing crab escapement require crab to push open a door on the topside of the trap, offset from the edge. Escapement effectiveness in these traps improved when equipped with a bungee to spring-load the door open upon escape cord degradation, but escape rates were still not maximized. The traps most successful at allowing crab escapement were those that provided an unobstructed escape panel either on the wall or along the topside edge of the trap. Traps not designed with this feature can be easily modified by detaching one escape ring, and re-attaching it with escape cord. Following escape cord degradation, the ring falls away resulting in an opening through which the crab can freely escape. This study provides the first reporting of escapement effectiveness from derelict crab traps of the region, and results are currently influencing resource management and gear manufacturing decisions with intent to reduce Dungeness crab mortality from derelict traps.

Response of Pacific halibut (Hippoglossus stenolepis) to future climate scenarios in the Northeast Pacific Ocean

Pacific halibut (Hippoglossus stenolepis) are a large-bodied species of flatfish that are important culturally, economically, and as a key predator in marine systems in the USA and Canada. The species has a wide distribution, and complex life history including large-scale migrations to spawn and feed, making it potentially susceptible to climate change impacts. We examined the potential changes in halibut distribution and relative abundance that may arise from changing temperature and dissolved oxygen concentrations using species distribution models (SDMs) and future climate scenarios downscaled by two regional ocean models. SDMs were fit with both environmental variables (depth, near-bottom temperature and dissolved oxygen concentration) and spatial random field components (representing unknown habitat-related variables). The best-fitting models, trained on data from 2009 to 2013, were able to account for 33 % and 53 % of the variation in small and large halibut catch-per-unit-effort in the annual set-line survey data from 2014 to 2020. The results suggest that the response of Pacific halibut to climate change in British Columbia and Washington waters is likely to depend on changes in dissolved oxygen concentration. Pacific halibut appear sensitive to changes in dissolved oxygen, yet relatively tolerant to increases in temperature. Projections for 2046–2065 period suggest that future decreases in near-bottom dissolved oxygen in shallow waters that small halibut inhabit are likely to result in moderate decreases in relative abundance. Projected changes in relative abundance are less certain for large Pacific halibut, due to disagreement in the regional ocean models near-bottom dissolved oxygen projections at mid depths (300–600 m) where large Pacific halibut are more common. Overall, the relative abundance of Pacific halibut is expected to decrease in most areas of British Columbia. Future management strategies will need to account for the projected changes in distribution and abundance and their uncertainty.

Isolation by Distance and Proximity to Urban Areas Affect Genetic Differentiation among Collections of English Sole (Parophrys vetulus, Family Pleuronectidae) in the Northeastern Pacific Ocean and Salish Sea

The English sole (Parophrys vetulus, family Pleuronectidae) is an abundant ground fish used as a sentinel of marine pollution in the inland marine waters of Washington and Oregon. We used 15 microsatellite loci to evaluate patterns of genetic variability within and among 17 collections of sole from coastal sites and within the southern Salish Sea. Over 470 alleles were identified in 15 loci, and high levels of genetic richness, as estimated by expected heterozygosity (HE) and allele richness (AR), were detected. Fish sampled from coastal areas at the northern end of our study area had the lowest values of genetic richness, in contrast to collections at the southern end. The level of differentiation among all collections was low: mean FST value was 0.0006. Nineteen comparisons had statistically significant FST values (mean FST of 0.0029) and involved comparisons involving three locations associated with urban areas (Port Gardner [Everett, WA], Sinclair Inlet [Bremerton, WA], and Commencement Bay [Tacoma, WA]). A statistically significant, but weak, isolation-by-distance pattern was seen in the five coastal collections over 1,100 km of coastline. In the Salish Sea collections, the pattern of differentiation was patchy and marked by several distinctive collections in close proximity to urban areas. The pattern of differentiation in the Salish Sea parallels differences seen in ecotoxicological characteristics of the English sole that inhabit separate urban embayments differing in pollution characteristics. Future work can focus on evaluating the stability and meaningfulness of this subtle variability in a time of local and global environmental changes.

Estimating the stock size of harbor seals (Phoca vitulina richardii) in the inland waters of Washington State using line-transect methods.

Harbor seals (Phoca vitulina richardii) in the inland waters of Washington were reduced by predator control programs in the twentieth century, but stocks have rebounded since being protected in the 1970s. Three management stocks are recognized, but there is little information on their current abundance. We conducted 38,431 km of aerial line-transect surveys throughout the range of these stocks in 2013–2016, sighting a total of 4,678 groups of harbor seals. Line-transect analysis with Beaufort sea state as a covariate provided estimates of the number of seals in the water. We then incorporated tagging data from 15 instrumented seals to develop correction factors, both for seals missed in the water while diving, and those that were on shore. Tagging data were modeled with generalized linear mixed models to provide estimates of the proportions diving and hauled out. After applying these correction factors, we estimated that the Hood Canal stock contained 1,368 seals (CV = 16.8%), the Southern Puget Sound stock contained 1,976 seals (CV = 20.5%), and the Washington Northern Inland Waters stock contained 7,513 seals (CV = 11.5%). This study presents a non-traditional approach to estimating the size of Washington inland waters harbor seal stocks, which may also be applicable to other species for which survey and tagging data are available.

Identifying local oil spill risk factors: An application of Hazard Identification within the International Maritime Organization Formal Safety Assessment framework in Washington State

Abstract Number 677002 Oil spills from commercial vessels are low probability, high consequence events that threaten economic, ecological, cultural, and natural resources. Washington State Department of Ecology Spill's Program (Ecology) has a robust risk assessment program that focuses on identifying and reducing oil spill risks to Washington waters. In 2017-2018, Ecology developed and implemented a novel application of the International Maritime Organization (IMO) Formal Safety Assessment process to lead a collaborative Hazard Identification for oil spill risk in Grays Harbor, Washington. This use of the IMO Formal Safety Assessment Process to assess oil spill risk was a first for Washington State and appears to be unique among US state governments. Working with area tribes, government agencies, and stakeholders, Ecology modified the IMO Formal Safety Assessment process to focus on local factors that could contribute to oil spill risks. Ecology facilitated structured brainstorming discussions during two workshops to complete Hazard Identification. Focusing on local factors fostered collaborative discussion among workshop participants, and allowed the process to benefit from local expertise about the characteristics of waterway and operational practices. The workshops resulted in the identification of 43 local factors related to oil spill risks, 34 recommendations to reduce risks based on current vessel traffic, and 10 recommendations to consider if vessel traffic increases in the future. The recommendations from this assessment are directly informing operations in Grays Harbor; in 2019, the Grays Harbor Safety Committee voted to adopt the risk assessment final report as an addendum to their Harbor Safety Plan. Ecology is also working with area tribes, government agencies, and stakeholders to review and prioritize the risk assessment recommendations with a goal of developing implementation plans for selected measures. This application of the IMO Formal Safety Assessment process represents a repeatable, scalable, and defensible method for conducting oil spill risk assessments. Ecology plans to use this process in other state waterways, and invites other organizations to consider adopting these methods. The presentation will walk through how to apply this process for localized waterway risk assessments and discuss best practices for success. Additionally, Ecology will discuss new risk analysis initiatives directed by the Washington State Legislature, including development of a quantitative model for evaluating oil spill risk and the potential effect of risk reduction measures.

Endangered predators and endangered prey: Seasonal diet of Southern Resident killer whales.

Understanding diet is critical for conservation of endangered predators. Southern Resident killer whales (SRKW) (Orcinus orca) are an endangered population occurring primarily along the outer coast and inland waters of Washington and British Columbia. Insufficient prey has been identified as a factor limiting their recovery, so a clear understanding of their seasonal diet is a high conservation priority. Previous studies have shown that their summer diet in inland waters consists primarily of Chinook salmon (Oncorhynchus tshawytscha), despite that species' rarity compared to some other salmonids. During other times of the year, when occurrence patterns include other portions of their range, their diet remains largely unknown. To address this data gap, we collected feces and prey remains from October to May 2004-2017 in both the Salish Sea and outer coast waters. Using visual and genetic species identification for prey remains and genetic approaches for fecal samples, we characterized the diet of the SRKWs in fall, winter, and spring. Chinook salmon were identified as an important prey item year-round, averaging ~50% of their diet in the fall, increasing to 70-80% in the mid-winter/early spring, and increasing to nearly 100% in the spring. Other salmon species and non-salmonid fishes, also made substantial dietary contributions. The relatively high species diversity in winter suggested a possible lack of Chinook salmon, probably due to seasonally lower densities, based on SRKW's proclivity to selectively consume this species in other seasons. A wide diversity of Chinook salmon stocks were consumed, many of which are also at risk. Although outer coast Chinook samples included 14 stocks, four rivers systems accounted for over 90% of samples, predominantly the Columbia River. Increasing the abundance of Chinook salmon stocks that inhabit the whales' winter range may be an effective conservation strategy for this population.

The Emergence of Mucormycosis in Free-Ranging Marine Mammals of the Pacific Northwest

Primary fungal diseases in marine mammals are rare. Mucormycosis, a disease caused by fungi of the order Mucorales, has been documented in few cetaceans and pinnipeds. In 2012, the first case of mucormycosis in the Pacific Northwest was documented in a dead stranded harbor porpoise (Phocoena phocoena) in Washington state. Since then, mucormycosis has been detected in a total of 21 marine mammals; fifteen harbor porpoises, five harbor seals (Phoca vitulina), and one southern resident killer whale (Orcinus orca). Infected animals were predominately found in the inland waters of Washington and British Columbia, and one harbor seal was recovered in northern Oregon. Fungal hyphae were detected histologically in a variety of tissues, including brain, lung, spleen, pancreas, kidneys, muscle, lymph nodes, and skin. Three fungal species were identified from seven cases by PCR screening or fungal culture; Rhizomucor pusillus (four cases), Lichtheimia corymbifera (two cases), and Cunninghamella bertholletiae. Underlying conditions such as emaciation, current or recent pregnancy, multisystemic parasitism, protozoal infection, and herpesvirus were found in several affected animals. Reasons for the appearance and subsequent increase of these fungal infections in marine mammals are unknown. The emergence of this disease as a source of marine mammal mortality in the Pacific Northwest is of particular concern for endangered southern resident killer whales that spend time in this region. Current population-level stressors such as insufficient prey, high levels of contaminants, and noise pollution, could predispose them to these fatal infections.

New Species of Lissodendoryx Topsent, 1892 (Demospongiae, Poecilosclerida, Coelosphaeridae) and Myxilla Schmidt, 1862 (Demospongiae, Poecilosclerida, Myxillidae) from the Northeast Pacific.

Collections of sponges by the late Dr. William C. Austin and the authors (N. McDaniel, R. Harbo and B. Ott) provided material for descriptions of new species from two genera of Poecilosclerida for shallow waters of Southern British Columbia, Canada and Northern Washington, USA: Lissodendoryx and Myxilla. There have been no new species of these two genera described for the Northeast Pacific since Laubenfels' work in central California (Laubenfels 1930, 1932) and Lambe's reports in 1893 to 1895 for Geological Survey of Canada sponge collections from British Columbia, Canada to the Bering Sea. We describe three new species of Lissodendoryx (Lissodendoryx) (Demospongiae, Poecilosclerida, Coelosphaeridae) and one new species of Myxilla (Myxilla) (Demospongiae, Poecilosclerida, Myxillidae): L. (L.) barkleyensis n. sp., L. (L.) littoralis n. sp., L. (L.) toxaraphida n. sp. and M. (M.) austini n. sp. Lissodendoryx (L.) barkleyensis n. sp. is cave-dwelling, has acanthostyles 112-260 µm, tornotes 107-177 µm, arcuate isochelas 8-28 µm and two sizes of sigmas 18-29, 26-55 µm. Lissodendoryx (L.) littoralis n. sp. fistulate habitus is adapted to muddy substrates similar to some Polymastia species also found commonly in the Northeast Pacific. It has subtylostyles 185-336 µm, tylotes 112-229 µm, arcuate isochelas 11-23 µm, and sigmas 30-75 µm. Lissodendoryx (L.) toxaraphida n. sp. is the only described Lissodendoryx species with raphides shaped like toxas. It has acanthostyles 140-286 µm, tornotes 143-195µm, arcuate isochelas 18-34 µm and toxiform raphides 65-156 µm. Myxilla (M.) austini n. sp. has a fistulate habitus and both tornote and tylote megascleres. It appears to be tolerant of low oxygen environments. Myxilla (M.) austini n. sp. has smooth to sparsely spined styles 193-353 µm, tylotes 153-221 µm, tornotes 174-260 µm, two sizes of anchorate isochelas 13-27, 42-81 µm, and two sizes of sigmas 13-47, 33-78 µm. All specimens were collected from shallow water (intertidal to 25 m).

Reproductive ecology and size-dependent fecundity in the petrale sole (Eopsetta jordani) in waters of California, Oregon, and Washington

Reproductive ecology and size-dependent fecundity in the petrale sole (Eopsetta jordani) in waters of California, Oregon, and Washington

Bycatch of rockfish in spot prawn traps and estimated magnitude of trap loss in Washington waters of the Salish Sea

Derelict fishing gear is a known stressor to rockfish populations in the Washington waters of the Salish Sea, including two species currently federally protected under the Endangered Species Act. In Washington and British Columbia, rockfish bycatch in actively fished (non-derelict) prawn traps has been documented in spot prawn test fisheries conducted by state and provincial government, and both live and dead rockfish have been found in derelict prawn traps encountered during derelict fishing gear removal operations in Washington. This study calculates rockfish bycatch rates in actively fishing prawn traps and provides preliminary trap loss rates for both commercial and recreational fisheries. Rockfish bycatch rates were determined through analysis of Washington Department of Fish and Wildlife (WDFW) Spot Prawn Test Fishery Data collected from 2004 to 2013. Data from WDFW creel surveys were used to update preliminary prawn trap loss rates. Interviews with WDFW marine enforcement officers were conducted to estimate the number of lost traps that are recovered before becoming derelict. The overall rockfish catch rates in Washington waters of the Salish Sea were 0.023 rockfish per trap drop, with considerable spatial and temporal variability. The lowest catch rates were consistently seen in Marine Area 7 (San Juan Islands and North Puget Sound); while the highest catch rates were seen in Marine Area 11 (south-central Puget Sound). The trap loss rate estimated for the recreational fishery is 2.33% of all traps fished. We estimate that over the years 2012 and 2013 an average of 653 recreational prawn traps became derelict each year. The accumulation of derelict prawn traps has a mostly unknown effect on benthic habitats of Puget Sound, which warrants additional research. While rockfish bycatch and prawn trap loss rates reported here are low, our findings support evaluating methods to reduce rockfish encounters with prawn traps.

Conception, fetal growth, and calving seasonality of harbor porpoise (Phocoenaphocoena) in the Salish Sea waters of Washington, USA, and southern British Columbia, Canada

We evaluated harbor porpoise (Phocoena phocoena (Linnaeus, 1758)) strandings in the Salish Sea to determine calving seasonality (1980–2015). A total of 443 strandings were analyzed, of which 134 were calves and 53 were neonates. Stranded calves were reported every month, but peaked in July, August, and September. Based on fetal size and an estimated fetal growth rate of 80 mm/month, mean (±SD) conception date (and range) was back-calculated to 11 October ± 30 days (16 August – 31 December) and was later than in most other studies. Using mean (±SD) length at birth (80 ± 5.8 cm), gestation was estimated to be approximately 10.8 months. Estimated birthing period was 16 July – 27 November, with a mean (±SD) birth date of 10 September (±30.7 days) and a birth length of 80.0 cm. Estimated pregnancy rate (0.28–0.29) is lower than reported in other areas and is likely an underestimate due to missed early embryos, poor postmortem condition of a large proportion of the stranded adult females, and potential biases related to the animals that strand and are available. This study of harbor porpoise reproduction and calving in the Salish Sea is the first assessment of calving seasonality for this species in the northeast Pacific Ocean.

Declining Marbled Murrelet density, but not productivity, in the San Juan Islands, Washington, USA

ABSTRACT The Marbled Murrelet (Brachyramphus marmoratus) is federally threatened in part of its range in western North America. Information on density and productivity is important for managing populations. Over an 18-yr period, we monitored murrelet density and productivity ratios during the breeding season along 170 km of shoreline of the San Juan Islands, Washington, USA. While murrelets occur throughout the coastal marine waters of Washington, the San Juan Islands support higher densities of murrelets during the breeding season than most other areas. From 1995 to 2012, the average density of adult murrelets declined significantly from 11.16 to 5.76 murrelets km−2, a decline that mirrored large-scale at-sea surveys in Washington. Despite this consistent and ominous decline in overall murrelet density, the density of juvenile murrelets and murrelet productivity ratio (juveniles:adults) did not decline over this time period. Thus, the declining density of murrelets in the San Juan Islands was due to decl...

Geography and host species shape the evolutionary dynamics of U genogroup infectious hematopoietic necrosis virus.

Infectious hematopoietic necrosis virus (IHNV) is a negative-sense RNA virus that infects wild and cultured salmonids throughout the Pacific Coastal United States and Canada, from California to Alaska. Although infection of adult fish is usually asymptomatic, juvenile infections can result in high mortality events that impact salmon hatchery programs and commercial aquaculture. We used epidemiological case data and genetic sequence data from a 303 nt portion of the viral glycoprotein gene to study the evolutionary dynamics of U genogroup IHNV in the Pacific Northwestern United States from 1971 to 2013. We identified 114 unique genotypes among 1,219 U genogroup IHNV isolates representing 619 virus detection events. We found evidence for two previously unidentified, broad subgroups within the U genogroup, which we designated 'UC' and 'UP'. Epidemiologic records indicated that UP viruses were detected more frequently in sockeye salmon (Oncorhynchus nerka) and in coastal waters of Washington and Oregon, whereas UC viruses were detected primarily in Chinook salmon (Oncorhynchus tshawytscha) and steelhead trout (Oncorhynchus mykiss) in the Columbia River Basin, which is a large, complex watershed extending throughout much of interior Washington, Oregon, and Idaho. These findings were supported by phylogenetic analysis and by FST. Ancestral state reconstruction indicated that early UC viruses in the Columbia River Basin initially infected sockeye salmon but then emerged via host shifts into Chinook salmon and steelhead trout sometime during the 1980s. We postulate that the development of these subgroups within U genogroup was driven by selection pressure for viral adaptation to Chinook salmon and steelhead trout within the Columbia River Basin.

Harbor porpoise (Phocoena phocoena) recovery in the inland waters of Washington: estimates of density and abundance from aerial surveys, 2013–2015

The harbor porpoise (Phocoena phocoena (L., 1758)) used to be common in Puget Sound, Washington, but virtually disappeared from these waters by the 1970s. We conducted systematic aerial line-transect surveys (17 237 km total effort) for harbor porpoises, with the goal of estimating density and abundance in the inland waters of Washington State. Surveys in Puget Sound occurred throughout the year from 2013 to 2015, and in the Strait of Juan de Fuca and the San Juan Islands (and some adjacent Canadian waters) in April 2015. We used a high-wing, twin-engine Partenavia airplane and four observers (one on each side of the plane, one looking through a belly port, and one recording data). A total of 1063 harbor porpoise groups were sighted. Density and abundance were estimated using conventional distance sampling methods. Analyses were limited to 447 harbor porpoise groups observed during 5708 km of effort during good sighting conditions suitable for line-transect analysis. Harbor porpoises occurred in all regions of the study area, with highest densities around the San Juan Islands and in northern Puget Sound. Overall, estimated abundance for the Washington Inland Waters stock was 11 233 porpoises (CV = 37%, 95% CI = 9 616 – 13 120). This project clearly demonstrated that harbor porpoises have reoccupied waters of Puget Sound and are present there in all seasons. However, the specific reasons for their initial decline and subsequent recovery remain uncertain.

Assessing potential spatial and temporal conflicts in Washington's marine waters

This paper uses spatial, temporal, and use-intensity data for 27 major marine uses in Washington waters to feature a method for assessing potential use conflicts in a variety of scenarios. The study represents a first step towards quantifying potential conflicts within Washington’s Marine Spatial Planning (MSP) study area by using a cumulative analysis to highlight high- and low-use intensity areas and the novel Marine Potential Conflict Index (MPCI), which incorporates space, time, and intensity of use, to quantify pairwise potential conflicts between uses. About 10% of pairs of uses do not overlap spatially and are likely compatible with one another. Temporally, the number of uses peaks in July and August and falls during January and February. Additionally, the MPCI identified three important use types with a substantial degree of potential conflict: commercial fishing, tribal fishing, and shipping. External data were used in three cases to ground-truth the analysis, as a limited test of its utility in managing any potential conflict. This article assesses the extent of Washington’s existing marine uses along spatial, temporal, and intensity axes, and more broadly provides a straightforward way to examine potential conflicts between marine uses.

Novel Finding of Widespread Occurrence and Accumulation of Bisphenol A Diglycidyl Ethers (BADGEs) and Novolac Glycidyl Ethers (NOGEs) in Marine Mammals from the United States Coastal Waters.

Bisphenol A diglycidyl ether (BADGE)- and bisphenol F diglycidyl ether (BFDGE)-based epoxy resins have a broad range of applications, including serving as inner coatings of food and beverage cans and as protective coatings in marine construction. Prior to this study, no studies had examined the occurrence and bioaccumulation of BADGEs or BFDGEs in aquatic organisms. In this study, BADGE, BFDGE, and nine of their derivatives were determined in 121 tissue (liver, kidney, blubber, and brain) samples from eight species of marine mammals collected from the U.S. coastal waters of Florida, California, Washington, and Alaska. BADGE·2HCl was the predominant compound found in the majority (78.5%) of the marine mammal tissues analyzed, at concentrations of up to 2950 ng/g (wet weight (wt)) found in the liver of a sea otter from Kachemak Bay, Alaska. The measured concentrations of BADGE·2HCl in marine mammals were on the order of hundreds of nanograms per gram tissue, which are some of the highest concentrations ever reported for this compound in biota. Males contained greater concentrations of BADGE·2HCl than did females. BADGE·2HCl also was found in the brain tissues of sea otters. Trace levels of BADGE·2HCl were found in the livers of polar bears from Alaska, which suggested that BADGEs are widely distributed in the oceanic environment.