Wall Shear Stress Levels Research Articles

Non-invasive measurement of wall shear stress in microfluidic chip for osteoblast cell culture using improved depth estimation of defocus particle tracking method.

The development of a non-invasive method for measuring the internal fluid behavior and dynamics of microchannels in microfluidics poses critical challenges to biological research, such as understanding the impact of wall shear stress (WSS) in the growth of a bone-forming osteoblast. This study used the General Defocus Particle Tracking (GDPT) technique to develop a non-invasive method for quantifying the fluid velocity profile and calculated the WSS within a microfluidic chip. The GDPT estimates particle motion in a three-dimensional space by analyzing two-dimensional images and video captured using a single camera. However, without a lens to introduce aberration, GDPT is prone to error in estimating the displacement direction for out-of-focus particles, and without knowing the exact refractive indices, the scaling from estimated values to physical units is inaccurate. The proposed approach addresses both challenges by using theoretical knowledge on laminar flow and integrating results obtained from multiple analyses. The proposed approach was validated using computational fluid dynamics (CFD) simulations and experimental video of a microfluidic chip that can generate different WSS levels under steady-state flow conditions. By comparing the CFD and GDPT velocity profiles, it was found that the Mean Pearson Correlation Coefficient is 0.77 (max = 0.90) and the Mean Intraclass Correlation Coefficient is 0.66 (max = 0.82). The densitometry analysis of osteoblast cells cultured on the designed microfluidic chip for four days revealed that the cell proliferation rate correlates positively with the measured WSS values. The proposed analysis can be applied to quantify the laminar flow in microfluidic chip experiments without specialized equipment.

Effects of hematocrit and non-Newtonian blood rheology on pulsatile wall shear stress distributions in vascular anomalies: A multiple relaxation time lattice Boltzmann approach

In the present study, we investigate the flow dynamics of non-Newtonian blood, focusing on the distribution of wall shear stress (WSS) and hematocrit levels, which is the volume percentage of red blood cells in whole blood. We analyze these factors under pulsatile conditions, in vascular anomalies such as stent channels and intracranial aneurysms. To achieve this, a three-dimensional computational approach based on the lattice Boltzmann method (LBM) with a multiple relaxation time (MRT) collision operator is employed. To represent the blood's shear-thinning properties, we developed a constitutive model inspired by the Carreau–Yasuda model. This model considers the variability in blood viscosity with shear rate correlated with hematocrit levels based on experimental data documented in the literature. The accuracy of the employed MRT-LBM is demonstrated by the consistency of results with analytical solutions for steady state and experimental data for pulsatile WSS distributions in non-Newtonian and Newtonian fluids. Results indicate that, in areas narrowed by stenosis or expanded by aneurysms, hematocrit levels affect flow dynamics. Higher hematocrit levels intensify pulsatile flow through stenotic regions, increasing WSS cyclic variations. We derived a density distribution function to demonstrate how shear rates vary in vascular anomalies, revealing blood viscosity changes and non-Newtonian properties. These properties complicate flow patterns, resulting in non-linear WSS distributions, which are essential for understanding endothelial cell reactions and disease pathways. Pulsatile blood flow and altered rheological properties due to increased hematocrit affect saccular aneurysm fluid dynamics over time and space, causing vorticities to change shape, size, and intensity.

Aerosol dosimetry in the whole conducting zone of a murine left-lung using CF-PD and LSFM images

Aerosol dosimetry in respiratory airways is relevant for pulmonary drug delivery and inhalation toxicology. Consequently, computational fluid-particle dynamics (CF-PD) modelling of pulmonary aerosol delivery is an active research field. Additionally, mice are the most commonly used animals in medical research. Technological advances have provided information on whole mice lung morphologies with unprecedented high resolution. Therefore, in this study, we used high-resolution light sheet fluorescent microscopy (LSFM) images of a healthy C57BL/6 mouse lung with a constant air flow rate of 72 ml/min, to extract an anatomical 3-dimensional (3D) geometry of the entire airway tree of the left lung from the primary bronchi to the most distal bronchioles excluding the trachea. The airways were segmented based on an order- and generation-based method. Also, to compare the morphological data and regional deposition, a generation-based investigation including 25 generations was employed in the present model. One-way coupling of CF-PD modeling was applied to model an intubated and mechanically-ventilated mouse. Maximum values of the velocity and vorticity magnitude of 3.2 m/s and 200,000 1/s were reached in the second order, respectively, and maximum pressure and wall shear stress levels were 30 Pa and 3.5 Pa, respectively. Finally, order- and generation-based particle deposition efficiency and dose per lung area were obtained for the particle size range of 1 μm ≤ dp ≤ 10 μm yielding pronounced hotspot deposition patterns mainly near the proximal bifurcations. The results showed a positive correlation between deposition efficiency and particle size due to a size-dependent increase in inertial and gravitational effects. Maximum regional deposition and normalized dose was seen for 10 μm particles in the 1st order of the murine left lung. Smaller peak sizes of deposition efficiency were seen in the third and fourth orders of the mouse left lung due to almost complete loss of the largest particles in lower order airways. It also justifies the close to zero deposition efficiency in the highest orders (fifth to sixth). Both lung morphology as well as total and regional aerosol deposition showed reasonably good agreement with empirical data from the literature. The present CF-PD model with accurate realistic lung morphology, improves our knowledge of airway aerosol deposition hotspots. The obtained modeling method and the qualitative results can be implemented on human airways.

The impact of choledochal cysts on bile fluid dynamics: A perspective using computational fluid dynamics and surface mapping technique

Choledochal cysts (CCs) are an important risk factor for cholangiocarcinoma, though their etiology remains debated. Given the vital role of bile fluid in digestive processes within the biliary system, examining such mechanisms from the perspective of bile fluid dynamics may offer additional insights for clinical use. This study utilized magnetic resonance imaging (MRI)-based patient-specific scans for detailed reconstruction and further employed the computational fluid dynamic method to assess the physiological functions of each system, including refilling and emptying processes. The impact of bile rheological property was also examined. Key biomechanical parameters—pressure and wall shear stress (WSS)—were displayed on a two-dimensional plane via surface mapping for enhanced visualization and comparative analysis. Outcomes demonstrated a significant reduction in bile flow velocity in CCs patients due to common bile duct's anatomical features and bile's shear-thinning, non-Newtonian nature, with a notable increase in pressure drop observed. In healthy biliary systems, WSS variations were minimal; however, in CCs patients, extreme WSS differences were found, with the highest WSS in the segmental bile duct and the lowest in the dilatation area, presenting a magnitude difference of approximately 1000. CCs one showed WSS levels 100–250 times higher than healthy ones in the common bile duct. Bile rheological properties substantially affect pressure and WSS patterns, particularly WSS, where pathological bile caused a tenfold increase in WSS compared to healthy bile. The findings aimed to enhance the understanding of biliary fluid mechanics in CCs and offer insights into selected fluidic variables for future microfluidic chip experiments.

Preventive maintenance of horizontal wind turbines via computational fluid dynamics-driven wall shear stress evaluation

Wind energy is a source of renewable energy that can be converted to electricity using a wind turbine. The typical lifespan of a wind turbine is at least 20–25 years. However, regular monitoring and preventive maintenance are required to ensure optimal performance and to extend the working life of wind turbines. Blade erosion and fatigue are the main structural issues which degrade wind turbine efficiency and lifespan. One method to identify the causes of these structural issues is computational fluid dynamics (CFD) that can be used to investigate relevant physical parameters affecting wind turbine performance. The current study investigated wall shear stress (WSS) or the force per area exerted by the wind close to the surfaces of the turbine blades and simulated the WSS at various points on the blade. The 3-parameter lognormal distribution that represented WSS probability density functions revealed that wind speed, rotational blade speed, and WSS were positively related, with the highest WSS levels occurring at the blade's leading edge, particularly in the region near the blade tip. Based on these findings, regular preventive maintenance plans should carefully monitor the blade leading edges, while protective coatings should be applied. These strategies would help to prevent blade erosion and fatigue, ultimately improving the efficiency and lifespan of the wind turbine. However, further research is needed to refine these CFD simulations and to validate the results based on additional experimental data.

Porosity and surface curvature effects on the permeability and wall shear stress of trabecular bone: Guidelines for biomimetic scaffolds for bone repair

Scaffolds are an essential component of bone tissue engineering to provide support and create a physiological environment for cells. Biomimetic scaffolds are a promising approach to fulfill the requirements. Bone allografts are widely used scaffolds due to their mechanical and structural characteristics. The scaffold geometry is well known to be an important determinant of induced mechanical stimulation felt by the cells. However, the impact of allograft geometry on permeability and wall shear stress distribution is not well understood. This information is essential for designing biomimetic scaffolds that provide a suitable environment for cells to proliferate and differentiate. The present study investigates the effect of geometry on the permeability and wall shear stress of bone allografts at both macroscopic and microscopic scales. Our results concluded that the wall shear stress was strongly correlated with the porosity of the allograft. The level of wall shear stress at a local scale was also determined by the surface curvature characteristics. The results of this study can serve as a guideline for future biomimetic scaffold designs that provide a mechanical environment favorable for osteogenesis and bone repair.

Enhancing Wind Turbine Blade Preventive Maintenance Procedure through Computational Fluid Dynamics-Based Prediction of Wall Shear Stress

Wind turbine blades are essential parts of wind energy systems and are frequently exposed to harsh environmental elements, such as strong winds, turbulence, and corrosive atmospheric elements. Over time, these circumstances may result in serious harm to blades, such as delamination and erosion, which may negatively affect the wind turbine’s functionality and durability. Accurate prediction of various types of damage is crucial to improve the toughness and lifespan of wind turbine blades and to maximize the overall effectiveness of wind energy systems. This article presents a novel computational fluid dynamics (CFDs)-based method for analyzing the distribution of wall shear stress on turbine blades, aimed at publicizing the yearly maintenance procedure. The investigation results from the CFDs, when compared with the current situation in a wind turbine farm in Thailand, confirmed that our wall shear stress modeling accurately predicted wind turbine damage. A maximum wall shear stress level higher than 5.00 Pa in the case of PA 90°, incoming air velocity 10.00 m/s, and 15 rpm was the main contribution to presenting the erosion and delamination from current drone inspection in wind turbine farms. In conclusion, these findings demonstrated the potential of using CFDs to predict wind turbine blade delamination and erosion, thereby significantly contributing to the development of specific and accurate yearly preventive maintenance. The proposed CFDs-based approach should serve as a sustainability tool for local human development, benefiting wind turbine engineers and operating technicians by providing them with a deeper understanding of the local flow conditions and wall shear stress distribution along wind turbine blades. This enables them to make informed decisions regarding blade design and maintenance.

Effects of hematocrit levels on flow structures and stress levels in the healthy and diseased carotid arteries

Flow structures and wall shear stress (WSS) distribution in human arteries depend strongly on blood rheology. Blood viscosity undergoes significant alterations due to varying hematocrit (Hct) levels. Hematocrit levels can change from the normal level (45%) due to numerous disorders such as leukemia, lymphoma, and congenital heart disease. However, the effects of Hct levels on the flow features and WSS levels in large stenotic arteries are not well reported in literature. The present study computationally assesses the variation in flow features in healthy and stenosed carotid arteries due to changes in blood Hct levels. We have reported variations in axial and secondary flow characteristics, vortex structures, and WSS-based parameters for different Hct levels. Specifically, we consider high (65%) and low (25%) Hct levels in addition to a normal Hct level. Our results reveal that both high and low Hct levels have adverse effects on the hemodynamic features inside a healthy carotid artery model. The low Hct level displays a much more widespread low time-averaged WSS (TAWSS) area, while the high Hct shows an increased oscillatory shear index (OSI) zone at the carotid sinus wall. In stenotic arteries, low Hct enhances the spread of the low TAWSS zone in the post-stenotic carotid sinus, whereas high Hct increases the risk of plaque rupture. The high Hct level also increases the spread of high OSI zone at the inner wall of internal carotid artery for the moderately stenosed artery.

Mechanical wall stress and wall shear stress are associated with atherosclerosis development in non-calcified coronary segments

Background and aimsAtherosclerotic plaque onset and progression are known to be affected by local biomechanical factors. Whereas the role of wall shear stress (WSS) has been studied, the impact of another biomechanical factor, namely mechanical wall stress (MWS), remains poorly understood. In this study, we investigated the association of MWS, independently and combined with WSS, towards atherosclerosis in coronary arteries. MethodsThirty-four human coronary arteries were analyzed using near-infrared spectroscopy intravascular ultrasound (NIRS-IVUS) and optical coherence tomography (OCT) at baseline and after 12 months. Baseline WSS and MWS were calculated using computational models, and wall thickness (ΔWT) and lipid-rich necrotic core size (ΔLRNC) change were measured in non-calcified coronary segments. The arteries were further divided into 1.5 mm/45° sectors and categorized as plaque-free or plaque sectors. For each category, associations between the biomechanical factors (WSS & MWS) and changes in the coronary wall (ΔWT & ΔLRNC) were studied using linear mixed models. ResultsIn plaque-free sectors, higher MWS (p < 0.001) was associated with greater vessel wall growth. Plaque sectors demonstrated wall thickness reduction over time, likely due to the medical therapy, where higher levels of WSS and WMS, individually and combined, (p < 0.05) were associated with a greater reduction. Sectors with low MWS combined with high WSS demonstrated the highest LRNC increase (p < 0.01). ConclusionsIn this study, we investigated the association of the (largely-overlooked) biomechanical factor, MWS with coronary atherosclerosis, individually and combined with WSS. Our results demonstrated that both MWS and WSS significantly correlate with atherosclerotic plaque initiation and development.

Wall shear stress and pressure patterns in aortic stenosis patients with and without aortic dilation captured by high-performance image-based computational fluid dynamics.

Spatial patterns of elevated wall shear stress and pressure due to blood flow past aortic stenosis (AS) are studied using GPU-accelerated patient-specific computational fluid dynamics. Three cases of moderate to severe AS, one with a dilated ascending aorta and two within the normal range (root diameter less than 4cm) are simulated for physiological waveforms obtained from echocardiography. The computational framework is built based on sharp-interface Immersed Boundary Method, where aortic geometries segmented from CT angiograms are integrated into a high-order incompressible Navier-Stokes solver. The key question addressed here is, given the presence of turbulence due to AS which increases wall shear stress (WSS) levels, why some AS patients undergo much less aortic dilation. Recent case studies of AS have linked the existence of an elevated WSS hotspot (due to impingement of AS on the aortic wall) to the dilation process. Herein we further investigate the WSS distribution for cases with and without dilation to understand the possible hemodynamics which may impact the dilation process. We show that the spatial distribution of elevated WSS is significantly more focused for the case with dilation than those without dilation. We further show that this focal area accommodates a persistent pocket of high pressure, which may have contributed to the dilation process through an increased wall-normal forcing. The cases without dilation, on the contrary, showed a rather oscillatory pressure behaviour, with no persistent pressure "buildup" effect. We further argue that a more proximal branching of the aortic arch could explain the lack of a focal area of elevated WSS and pressure, because it interferes with the impingement process due to fluid suction effects. These phenomena are further illustrated using an idealized aortic geometry. We finally show that a restored inflow eliminates the focal area of elevated WSS and pressure zone from the ascending aorta.

CARD3: Wall Shear Stress Signature of the Axial B-impeller Design with Superior Hemodynamics

Purpose: Ventricular assist devices (VADs) present great promise as both bridge-to-transplant and destination therapies for patients with end-stage heart failure. However, complications—such as hemolysis, gastrointestinal bleeding, and degradation of the von Willebrand factor—stand in the way of their widespread application. High levels of wall shear stress resulting from poor hemodynamics within the bladed flow domain are primarily responsible. Computational fluid dynamics (CFD) software was used to map the hemodynamic performance of the B-impeller—a biologically-inspired, helicoid VAD impeller design—and provide detailed insight into the internal flow structures, leading to the shear stress levels within. Methods: The B-impeller with casing was parametrically modeled using a computer aided design software. The geometry was exported to a CFD software and simulations were subsequently run to assess the impeller hemodynamics with respect to the wall shear stress parameter. Results: The B-impeller was found to have a very low shear stress signature versus typical VAD designs, which exhibit much higher signatures than the defined hemolytic threshold of 400 Pascals. The peak and area-averaged values of shear stress were found to be 304.512 Pa (at a flow rate of 9 L/min) and 39.949 Pa (averaged over multiple input flow rate simulations), respectively. Conclusion: The B-impeller concept shows great potential to reduce the adverse hemolytic events associated with most other turbo VADs, in addition to its ability to generate high rates of flow in a minimally intrusive diameter of 8 mm. This biologically inspired, rim-driven impeller design was found to reduce shear stress levels over competitive designs by virtue of its radical, open architecture. This low shear stress signature translates to lowering rates of hemolysis, bleeding, and von Willebrand factor degradation, thereby improving post-implantation outcomes of patients.

How spanwise travelling transversal surface waves change the near-wall flow

The alteration of the near-wall flow field of a turbulent boundary layer flow subjected to spanwise travelling transversal surface waves at a friction Reynolds number $Re_\tau \approx 1525$ is investigated. The results of a spatial noise-assisted multivariate empirical mode decomposition reveal that this flow control method periodically induces near-wall large-scale bursts while simultaneously lowering the energetic content of small-scale features. The increasing occurrence of intense large-scale ejections in the near-wall region is of particular importance for reducing the wall-shear stress since these ejections balance large-scale sweeps originating from the outer layer. Thus, they corrupt the outer-layer impact on the near-wall dynamics and, consequently, the overall fluctuation intensity at the wall is attenuated. This disturbed top-down momentum exchange is highlighted by an inner–outer interaction analysis, which further reveals an increased bottom-up communication provoked by the large-scale ejections. Moreover, it is shown that the periodic secondary flow field induced by the actuation interferes with the quasi-streamwise vortices in the near-wall region. The velocity gradients of the secondary flow field deform the vortices’ cross-section into an elliptic shape, which yields an unstable vortex state resulting in vortex disintegration. In combination with the effect of the large-scale ejections, the reduced number of quasi-streamwise vortices compared with the undisturbed boundary layer flow results in a decreased wall-normal momentum exchange and the widening and weakening of near-wall streaks. This yields a reduced fluctuation intensity in the near-wall region that lowers the overall wall-shear stress level.

Impact of Fe2+ and Shear Stress on the Development and Mesoscopic Structure of Biofilms-A Bacillus subtilis Case Study.

Bivalent cations are known to affect the structural and mechanical properties of biofilms. In order to reveal the impact of Fe2+ ions within the cultivation medium on biofilm development, structure and stability, Bacillus subtilis biofilms were cultivated in mini-fluidic flow cells. Two different Fe2+ inflow concentrations (0.25 and 2.5 mg/L, respectively) and wall shear stress levels (0.05 and 0.27 Pa, respectively) were tested. Mesoscopic biofilm structure was determined daily in situ and non-invasively by means of optical coherence tomography. A set of ten structural parameters was used to quantify biofilm structure, its development and change. The study focused on characterizing biofilm structure and development at the mesoscale (mm-range). Therefore, biofilm replicates (n = 10) were cultivated and analyzed. Three hypotheses were defined in order to estimate the effect of Fe2+ inflow concentration and/or wall shear stress on biofilm development and structure, respectively. It was not the intention to investigate and describe the underlying mechanisms of iron incorporation as this would require a different set of tools applied at microscopic levels as well as the use of, i.e., omic approaches. Fe2+ addition influenced biofilm development (e.g., biofilm accumulation) and structure markedly. Experiments revealed the accumulation of FeO(OH) within the biofilm matrix and a positive correlation of Fe2+ inflow concentration and biofilm accumulation. In more detail, independent of the wall shear stress applied during cultivation, biofilms grew approximately four times thicker at 2.5 mg Fe2+/L (44.8 µmol/L; high inflow concentration) compared to the low Fe2+ inflow concentration of 0.25 mg Fe2+/L (4.48 µmol/L). This finding was statistically verified (Scheirer-Ray-Hare test, ANOVA) and hints at a higher stability of Bacillus subtilis biofilms (e.g., elevated cohesive and adhesive strength) when grown at elevated Fe2+ inflow concentrations.

Bactericidal Efficacy of Nanostructured Surfaces Increases under Flow Conditions.

Bacterial colonization on solid surfaces creates enormous problems across various industries causing billions of dollars' worth of economic damages and costing human lives. Biomimicking nanostructured surfaces have demonstrated a promising future in mitigating bacterial colonization and related issues. The importance of this non-chemical method has been elevated due to bacterial evolvement into antibiotic and antiseptic-resistant strains. However, bacterial attachment and viability on nanostructured surfaces under fluid flow conditions has not been investigated thoroughly. In this study, attachment and viability of Pseudomonas aeruginosa (P. aeruginosa) and Staphylococcus aureus (S. aureus) on a model nanostructured surface were studied under fluid flow conditions. A wide range of flow rates resulting in a broad spectrum of fluid wall shear stress on a nanostructured surface representing various application conditions were experimentally investigated. The bacterial suspension was pumped through a custom-designed microfluidic device (MFD) that contains a sterile Ti-6Al-4V substrate. The surface of the titanium substrate was modified using a hydrothermal synthesis process to fabricate the nanowire structure on the surface. The results of the current study show that the fluid flow significantly reduces bacterial adhesion onto nanostructured surfaces and significantly reduces the viability of adherent cells. Interestingly, the bactericidal efficacy of the nanostructured surface was increased under the flow by ∼1.5-fold against P. aeruginosa and ∼3-fold against S. aureus under static conditions. The bactericidal efficacy had no dependency on the fluid wall shear stress level. However, trends in the dead-cell count with the fluid wall shear were slightly different between the two species. These findings will be highly useful in developing and optimizing nanostructures in the laboratory as well as translating them into successful industrial applications. These findings may be used to develop antibacterial surfaces on biomedical equipment such as catheters and vascular stents or industrial applications such as ship hulls and pipelines where bacterial colonization is a great challenge.

Wall Shear Stress Analysis and Optimization in Tissue Engineering TPMS Scaffolds.

When designing scaffolds for bone tissue engineering (BTE), the wall shear stress (WSS), due to the fluid flow inside the scaffold, is an important factor to consider as it influences the cellular process involved in new tissue formation. The present work analyzed the average WSS in Schwartz diamond (SD) and gyroid (SG) scaffolds with different surface topologies and mesh elements using computational fluid dynamics (CFD) analysis. It was found that scaffold meshes with a smooth surface topology with tetrahedral elements had WSS levels 35% higher than the equivalent scaffold with a non-smooth surface topology with hexahedral elements. The present work also investigated the possibility of implementing the optimization algorithm simulated annealing to aid in the design of BTE scaffolds with a specific average WSS, with the outputs showing that the algorithm was able to reach WSS levels in the vicinity of 5 mPa (physiological range) within the established limit of 100 iterations. This proved the efficacy of combining CFD and optimization methods in the design of BTE scaffolds.

The Effect of the Operation of Downhole Equipment on the Processes of Corrosive Wear (by the Example Inflow Control Devices of Nozzle Type).

Existing approaches to assessing the reliability and safe operation of downhole equipment provide for assessing only the direct impact of erosion and corrosion processes on the degradation of the material. At the same time, the influence of downhole equipment operating modes on the intensity of corrosion and erosion processes is not evaluated. The necessity of using inflow control devices is shown. The necessity of selecting inflow control devices for specific operating modes of the well is highlighted in order to avoid increased erosion wear due to increased sand production. In this article, a series of studies was conducted to assess the influence of the hydrodynamic characteristics of the fluid flow, which vary depending on the operating modes of the nozzle-type inflow control device, on corrosion processes in the well. It was shown that the level of wall shear stress (WSS) of the base pipe, immediately after the flow control device in the direction of fluid flow, affects the intensification of corrosion processes in downhole equipment. Studies of WSS on the pipe wall were carried out using a dynamic autoclave and elements of mathematical modeling. The design factors (the angle of inclination of the main hydraulic channel of the inflow control device relative to the base pipe) affecting the change in tangential stresses on the wall of the base pipe were investigated. The mechanism of corrosion wear of the base pipe was revealed.

Fluid mechanical performance of ureteral stents: The role of side hole and lumen size.

Ureteral stents are indispensable devices in urological practice to maintain and reinstate the drainage of urine in the upper urinary tract. Most ureteral stents feature openings in the stent wall, referred to as side holes (SHs), which are designed to facilitate urine flux in and out of the stent lumen. However, systematic discussions on the role of SH and stent lumen size in regulating flux and shear stress levels are still lacking. In this study, we leveraged both experimental and numerical methods, using microscopic-Particle Image Velocimetry and Computational Fluid Dynamic models, respectively, to explore the influence of varying SH and lumen diameters. Our results showed that by reducing the SH diameter from to the median wall shear stress levels of the SHs near the ureteropelvic junction and ureterovesical junction increased by over , even though the flux magnitudes through these SH decreased by about . All other SHs were associated with low flux and low shear stress levels. Reducing the stent lumen diameter significantly impeded the luminal flow and the flux through SHs. By means of zero-dimensional models and scaling relations, we summarized previous findings on the subject and argued that the design of stent inlet/outlet is key in regulating the flow characteristics described above. Finally, we offered some clinically relevant input in terms of choosing the right stent for the right patient.

High-Throughput Imaging of Blood Flow Reveals Developmental Changes in Distribution Patterns of Hemodynamic Quantities in Developing Zebrafish.

Mechanical forces from blood flow and pressure (hemodynamic forces) contribute to the formation and shaping of the blood vascular network during embryonic development. Previous studies have demonstrated that hemodynamic forces regulate signaling and gene expression in endothelial cells that line the inner surface of vascular tubes, thereby modifying their cellular state and behavior. Given its important role in vascular development, we still know very little about the quantitative aspects of hemodynamics that endothelial cells experience due to the difficulty in measuring forces in vivo. In this study, we sought to determine the magnitude of wall shear stress (WSS) exerted on ECs by blood flow in different vessel types and how it evolves during development. Utilizing the zebrafish as a vertebrate model system, we have established a semi-automated high-throughput fluorescent imaging system to capture the flow of red blood cells in an entire zebrafish between 2- and 6-day post-fertilization (dpf). This system is capable of imaging up to 50 zebrafish at a time. A semi-automated analysis method was developed to calculate WSS in zebrafish trunk vessels. This was achieved by measuring red blood cell flow using particle tracking velocimetry analysis, generating a custom-made script to measure lumen diameter, and measuring local tube hematocrit levels to calculate the effective blood viscosity at each developmental stage. With this methodology, we were able to determine WSS magnitude in different vessels at different stages of embryonic and larvae growth and identified developmental changes in WSS, with absolute levels of peak WSS in all vessel types falling to levels below 0.3 Pa at 6 dpf. Additionally, we discovered that zebrafish display an anterior-to-posterior trend in WSS at each developmental stage.

The accumulation of particles in ureteric stents is mediated by flow dynamics: Full-scale computational and experimental modeling of the occluded and unoccluded ureter.

Ureteric stents are clinically deployed to restore urinary drainage in the presence of ureteric occlusions. They consist of a hollow tube with multiple side-holes that enhance urinary drainage. The stent surface is often subject to encrustation (induced by crystals-forming bacteria such as Proteus mirabilis) or particle accumulation, which may compromise stent's drainage performance. Limited research has, however, been conducted to evaluate the relationship between flow dynamics and accumulation of crystals in stents. Here, we employed a full-scale architecture of the urinary system to computationally investigate the flow performance of a ureteric stent and experimentally determine the level of particle accumulation over the stent surface. Particular attention was given to side-holes, as they play a pivotal role in enhancing urinary drainage. Results demonstrated that there exists an inverse correlation between wall shear stress (WSS) and crystal accumulation at side-holes. Specifically, side-holes with greater WSS levels were those characterized by inter-compartmental fluid exchange between the stent and ureter. These “active” side-holes were located either nearby ureteric obstructions or at regions characterized by a physiological constriction of the ureter. Results also revealed that the majority of side-holes (>60%) suffer from low WSS levels and are, thus, prone to crystals accumulation. Moreover, side-holes located toward the proximal region of the ureter presented lower WSS levels compared to more distal ones, thus suffering from greater particle accumulation. Overall, findings corroborate the role of WSS in modulating the localization and extent of particle accumulation in ureteric stents.

Hemodynamic study of the effect of the geometric height of leaflets on the performance of the aortic valve under aortic valve reconstruction

BackgroundAlthough aortic valve reconstruction has become an alternative treatment for aortic valve disease, the design of the geometric parameters of the reconstructed leaflet still mainly depends on the experience of doctors. The present study investigates the effects of the height of the leaflets on the performance and biomechanical states of the reconstructed aortic valve.MethodsThis numerical study was carried out using the finite element approach and the lattice Boltzmann method. The dynamic and biomechanical characteristics of the leaflets were evaluated by using the finite element approach, while the blood flow in the aortic sinus was evaluated by applying the lattice Boltzmann method. Three types of leaflets with different heights were designed. Then the dynamic characteristics, stress distribution, and effective orifice area (EOA) of the aortic valve and flow pattern were calculated as the indicators.ResultsThe results demonstrated that the height of the leaflets could indeed regulate the performance and the biomechanical states of the aortic valve. The rapid valve opening times of the 3 types of leaflets gradually reduced along with the decrease of the height ratio (HR_0.8: 120 ms vs. HR_1.0: 68 ms vs. HR_1.2: 31 ms), while the rapid valve closing times (RVCTs) of the 3 types of leaflets were similar to each other (approximately 75 ms). Moreover, the radial displacement of the leaflet at the fully open time increased along with the decrease of the HR of the leaflets (HR_0.8: 8 mm vs. HR_1.0: 6 mm vs. HR_1.2: 4 mm). In addition, the stress level of the leaflets also increased with the increase of the height of the leaflets (max stress, HR_0.8: 0.5 MPa, vs. HR_1.0: 1.1 MPa, vs. HR_1.2: 1.8 MPa). Similarly, the low velocity region near the ascending aortic wall and the wall shear stress (WSS) level on the ventricular side of the leaflets also increased along with the increase of the HR of the leaflets.ConclusionsIn short, the height of the leaflets mainly affects the opening performance of the reconstructed aortic leaflets. The HR of the reconstructed leaflets for adults should be less than 1.0 to balance the opening and closing performance of aortic leaflets.