Recent innovations in physical activity (PA) assessment have made it possible to assess the walking behaviors of a wide variety of populations. Objective measurement methods (e.g., pedometers, accelerometers) have been widely used to assess walking and other prevalent types of PA. Questionnaires suitable for international populations (e.g., the International Physical Activity Questionnaire and the Global Physical Activity Questionnaire) and measurement techniques for the assessment of gait patterns in disabled populations allow for the study of walking and its health benefits among many populations. Results of studies using the aforementioned techniques indicate that children are more active than adolescents and adolescents are more active than adults. Males, particularly young males, are typically more active than females. The benefits associated with regular participation in PA for youth and walking for older adults have been well documented, although improvements in the assessments of physical, cognitive, and psychosocial parameters must be made if we are to fully understand the benefits of walking for people of all ages. Most youth meet appropriate age-related PA activity recommendations, but adults, particularly older adults and adults with disabilities, are less likely to meet PA levels necessary for the accrual of health benefits. International studies indicate variation in walking by culture. It is clear, however, that walking is a prevalent form of PA across countries and a movement form that has great potential in global PA promotion. Continued development of measurement techniques that allow for the study of individualized gait patterns will help us add to the already rich body of knowledge on chronically disabled populations and allow for individual prescriptions for these populations.