Maize soil pathogenic fungi, Fusarium moniliforme, Macrophomina phaseolina, and Rhizoctonia zeae are important causes of annual economic losses worldwide. Since there are no maize cultivars with complete resistance to soil-borne fungal pathogens. This study aims to produce highly effective Nano-formulations of Peganum harmala and Ailanthus altissima plant extracts to control maize soil fungi. The GC-MS, DLS, Zeta, TEM, SEM, FTIR, disease symptoms, fresh and dry weights, Catalase, and Peroxidase enzymes were measured under in-vitro and in-vivo conditions. Harmine and Quercetin were the most common compounds in the extracts. The obtained results showed that the particles were spherical and encapsulated on a nanoscale. The in-vitro results showed that the best treatment for the F. moniliforme, M. phaseolina, and R. zeae fungi was P. harmala Nanoemulsion at 20 % concentration with 78.7 % inhibition. Maize seed priming with 20 % P. harmala Nanoemulsion showed the highest fresh and dry weights of 75.2 and 25.2 g, respectively, with minimal disease symptoms. The highest activity of Catalase and Peroxidase, 6.2 and 2.4 U mg protein−1 min−1, respectively.