Voucher Number Research Articles

Macromycetes Determined in Aluçdağı Nature Park (Çamlıdere-Ankara) and its Close Environs

The study is based on the macrofungi samples collected from Aluçdağı Nature Park and its environs between the years 2021 and 2024. As a result of field and laboratory studies, 124 macrofungi species belonging to 2 divisions, 4 classes, 15 orders, 50 families and 86 genera were determined. The list of the species is presented together with their localities, habitat or substrates, collection dates and voucher numbers.

Medicinal plants used by traditional medicine practitioners in treatment of alcohol-related disorders in Bushenyi District, southwestern Uganda.

Alcohol-related disorders rank seventh among risk factors for morbidity and mortality globally, posing a significant public health burden. In Africa, including Uganda, there is limited availability and utilization of pharmacotherapies to treat alcohol-related disorders. This study documented medicinal plant species, plant parts used, and the methods of preparation and administration utilized by Traditional Medicine Practitioners (TMPs) in treating alcohol-related disorders in southwestern Uganda. A descriptive cross-sectional ethnopharmacological survey was conducted among TMPs within Bushenyi District, southwestern Uganda. Data was collected with key informant interviews using semi-structured questionnaires. The TMPs identified medicinal plants by local names. Plant specimens were collected and deposited at the Department of Biology, Faculty of Science, Mbarara University for identification and voucher numbers allocated. The plant scientific names and species were identified based on the International Plant Names Index. Plant species, family, life form, number of mentions, method of collection, preparation and administration were analyzed using descriptive statistics in Microsoft Excel. The survey data were utilized to compute Frequency of Citation, Relative Frequency of Citation, and Informant Consensus Factor. We enrolled 50 traditional medicine practitioners aged between 34 and 98years, with a mean age of 67. Approximately two-thirds were female (66%, 33/50), and mean experience in traditional healing was 31years. The total number of plants identified were 25 belonging to 20 families. The most prevalent plant life form was herbs (36%) while grasses (4%), were the least. Leaves (48%) were the most utilized plant parts with the least utilized being the barks. The most prevalent method, adopted by approximately one-third of the TMPs, involved drying the plant material in the sun. The Informant Consensus Factor was 0.67. The study shows that the traditional medicine practitioners in Bushenyi district use a wide diversity of plants species to treat alcohol related disorders. The relatively high Informant Consensus Factor suggests a significant level of agreement among TMPs regarding the use of the identified plants. We recommend further investigations into phytochemistry, safety, efficacy, and mechanisms of action of the identified plants.

A Contribution to the Ethnobotany of Dhule and Thane Districts of Maharashtra State (India) - A Preliminary Report

Native medicinal uses of 11 plants common among Tadvi, Kokni & Gavit tribes inhabiting Dhule district and Katkari, Varli & Koli tribes of Thane district of Maharashtra State are reported. These plants have either not been reported by earlier workers or their uses mentioned here are unrecorded. Information is given on local name, locality, voucher number, native uses and mode of administration of doses.

Macromycetes determined in Yıldız Mountain (Sivas)

In this study macrofungi determined at Yıldız Mount (Sivas-Türkiye), through the identification on the samples collected between 2019-2020, are presented. As a result of field and laboratory studies, 62 species within 26 families were identified. Four of them belong to the division Ascomycota and 58 to Basidiomycota. The determined species are listed together with their collection localities, habitats, edibility status and voucher numbers.

Macromycetes determined in Baykan (Siirt) District

The current study was based on the macrofungi collected from Baykan (Siirt) district. As a result of field and laboratory studies, 88 species belonging to 56 families were identified. Nine taxa belong to Ascomycota and 79 to Basidiomycota. Considering the taxonomic categories of the species identified in the research area, it is seen that the order, family and genus represented by the most members are Agaricales, Agaricaceae and Agaricus L., respectively. The identified species are listed along with their localities, habitats/substrates, geographical positions, collection dates, and voucher numbers. All of the determined species are new records for the study area.

Genetic variations and phylogenetic relationship of genus Uromastyx from Punjab Pakistan.

During the present study, specimens were collected from selected sites of Cholistan desert and Kalabagh Game Reserve, Punjab province, Pakistan. Each captured specimen was tagged with voucher number and morphometric measurements were taken. The average snout to vent length was 172.559±1.40 mm and average weight was 92.1±1.30 g. The DNA of Uromastyx hardwickii was amplified and sequenced using 16S rRNA primer set. The obtained DNA sequence has shown reliable and clear species identification. After trimming ambiguous bases, the obtained 16S rRNA fragment was 520 bp while 16S rRNA fragments aligned with closely matched sequence from NCBI comprised of 510 bp. Closely matched sequences of genus Uromastyx were retrieved from NCBI in blast searches. Neighbour-joining tree of genus Uromastyx was constructed based on p-distance using MEGA X. The mean intraspecific variation was 0.095±0.01 while intraspecific variation was ranging from 0-1%. Similarly, interspecific variation of Uromastyx hardwikii with Saara asmussi, Uromastyx alfredschmidti, Uromastyx geyri, Uromastyx thomasi, Uromastyx alfredschmidti was 0-12%, 0-19%, 0-19%, 0-20%, 12-19% respectively. The newly produced DNA was submitted to NCBI and accession number was obtained (MW052563.1). Results of current study provided information about the molecular and morphological identification of Genus Uromastyx. In our recommendation, comprehensive molecular based identification of Pakistan's reptiles is required to report any new or subspecies from country.

Angiosperm weeds diversity of Kohima District, Nagaland, Northeast India

The paper presents a comprehensive study of 118 common angiosperm weeds from Kohima District, Nagaland. These weeds represent 33 families and 92 genera. The botanical names, vernacular names, common names, family, habit, lifespan, voucher number and uses are enumerated systematically. The study reveals that certain plant families predominate in the study area, with Compositae (19.49%), Leguminosae (16.95%), and Poaceae (7.63%) being the most dominant. These families have a significant presence among the recorded weeds. Herbaceous plants were represented with the highest number of species (68%), followed by shrubs (16%), under shrubs (10%), climbers and vines (2%), creeping herb and lianas (1%). The paper also highlights the utilization of some weed species as medicines and vegetables for consumption by the local population as well as fodder for animals. The current study will provide an inventory of the common angiosperm weeds in Kohima District providing a valuable resource for local biodiversity preservation and also help researchers, scientists, farmers, and students who want to conduct further studies in this field.

Macromycetes determined in Çamlı (Of-Trabzon) village and its environs

The study was carried out on the macrofungi samples collected from Çamlı (Of-Trabzon) district and its close environs. As a result, 118 macrofungi species within 63 genera, 38 families, 8 orders and 3 classes belonging to Ascomycota and Basidiomycota were determined. Ten of the determined species belong to Ascomycota while 108 belong to Basidiomycota. In the region, the most crowded order is Agaricales, the most crowded families are Inocybaceae and Psathyrellaceae, and the most crowded genus is Inocybe in terms of taxa number. The determined species are listed together with their localities, habitats/substrates, geographical position, collection date and voucher numbers.

Contribution to the Macrofungal Biodiversity of Yahyalı District

This contributory study was carried out on the macrofungi samples collected from Yahyalı district of Kayseri province. As a result, 69 macromycete species belonging to 56 genera, 35 families and 7 orders within Agaricomycetes and Pezizomycetes were determined. Including the previously reported species, a total of 147 macromycete species were compiled from the region. The list of the determined species was provided together with their habitats, collection dates, voucher numbers, or the citations for those reported before.

Medicinal plants of the upper Aswa River catchment of northern Uganda - a cultural crossroads

BackgroundThis paper presents a comparative inventory of medicinal plant taxa and their uses by smallholder farming communities of four cultures in the Aswa River catchment of northern Uganda, situated in the eastern Sudanian savanna parkland ecotype of sub-Saharan Africa. The purpose of the study was to document the ethnobotanical use of medicinal plants by the Lango, Acholi, Teso (Atesot) and Ethur (jo Abwor), in an historical moment before civil conflict and mass displacement of the respondent communities disrupted the inter-generational transmission of traditional technical knowledge within the study area.MethodsFollowing community consultations in four districts of northern Uganda during 1999–2000, interviews were conducted with holders of specialist knowledge on plants used as medicine on basis of a plant specimen allocated a voucher number and identified by the national herbarium. Use reports reflecting specific medicinal applications were compiled in aggregate to obtain a Relative Importance Index ranking. The commonality of medicinal taxa cited between each cultural interface was assessed by the Jaccard Index of Similarity, and the similarity of specific medicinal usage by taxon using Rahman’s Similarity Index.ResultsThe data collected from 112 respondents comprise 280 medicinal use reports describing 263 applications for 62 medical conditions, citing 108 taxa from 44 botanical families of which Fabaceae comprised 20% of all use reports. No earlier mention could be found to corroborate 72 use reports (27% of the total), representing medicinal indications as yet undocumented, and potentially worthy of investigation. The RI values ranged between 15 and 94%, with 13 taxa having RI values above 50%. The JI ratios indicate the highest degree of similarity in the plant taxa used as medicine (21%) between the Lango and Teso cultures who share a common origin; however, Rahman’s Similarity Index indicates the highest similarity of specific medicinal usage by taxon between the Lango and Acholi, who share a common language group through cultural assimilation over time. ConclusionsAs a comparative study, the results imply that cultural exchange and assimilation may be a greater driver of inter-cultural similarity of ethnopharmacological use of a given taxon, as compared to shared historical origins.

New Species for Regional Mycobiotas of Russia. 8. Report 2023

A total of 83 fungal species including one ascomycete (Helvella macropus) and 82 basidiomycete species have been recorded for the first time from 18 administrative regions of Russia: Republic of Buryatia (2), Republic of Dagestan (7), Republic of Mordovia (30), Republic of Tyva (6), Primorsky Krai (1), Zabaykalsky Krai (3), Arkhangelsk Oblast (5), Irkutsk Oblast (4), Kaluga Oblast (1), Leningrad Oblast (1), Murmansk Oblast (7), Nizhny Novgorod Oblast (1), Novgorod Oblast (3), Novosibirsk Oblast (2), Pskov Oblast (1), Tyumen Oblast (10), Saint Petersburg Federal City (1), and Khanty-Mansi Autonomous Okrug (1 species). An annotated species list containing the data on locality, substrate, habitat type, date of collection, and voucher numbers is provided. Pluteus vellingae is reported as the first record in Russia. Aurantiporus priscus, Bolbitius sibiricus, and Mycopan scabripes are recorded in Russia for the second time. Hypsizygus marmoreus is reported for the third time. New data on little-collected fungal species such as Botryobasidium botryoideum, Fibrodontia gossypina, Lycoperdon rupicola, Neohypochnicium cremicolor, Postia luteocaesia, Psathyrella gordonii, and Xylodon pruinosus, is presented. Complete sequences of ITS1–5.8S–ITS2 nuclear ribosomal DNA for 16 species reported have been generated and submitted to the GenBank database.

An annotated plant checklist of the transboundary volcanic Mt Elgon, East Africa.

Mt Elgon is an ancient transboundary volcanic mountain found at the Kenya-Uganda boarder possessing high plant diversity. This study documents an updated checklist of the mountain's vascular plants obtained through random-walk field excursions and retrieval of herbarium specimen tracing back to 1900. We compiled 1709 species from 673 genera in 131 families. One new species of the family Cucurbitaceae was also reported. This checklist records respective habitat, habits, elevation ranges, voucher numbers and global distribution ranges of each species. Native and exotic species were also distinguished, where 8.4% of the total species in 49 families were exotic species. There were 103 endemic species, while 14 species were found to be both rare and endemic. IUCN conservation status revealed 2 Critically Endangered, 4 Endangered, 9 Vulnerable and 2 Near Threatened species. This study presents the first and most comprehensive plant inventory of Mt Elgon that will facilitate further ecological and phylogenetic studies.

Network Pharmacology and Molecular Modeling to Elucidate the Potential Mechanism of Neem Oil against Acne vulgaris.

Acne vulgaris is a common skin disorder with a complicated etiology. Papules, lesions, comedones, blackheads, and other skin lesions are common physical manifestations of Acne vulgaris, but the individual who has it also regularly has psychological repercussions. Natural oils are being utilized more and more to treat skin conditions since they have fewer negative effects and are expected to provide benefits. Using network pharmacology, this study aims to ascertain if neem oil has any anti-acne benefits and, if so, to speculate on probable mechanisms of action for such effects. The neem leaves (Azadirachta indica) were collected, verified, authenticated, and assigned a voucher number. After steam distillation was used to extract the neem oil, the phytochemical components of the oil were examined using gas chromatography-mass spectrometry (GC-MS). The components of the oil were computationally examined for drug-likeness using Lipinski's criteria. The Pharm Mapper service was used to anticipate the targets. Prior to pathway and protein-protein interaction investigations, molecular docking was performed to predict binding affinity. Neem oil was discovered to be a potential target for STAT1, CSK, CRABP2, and SYK genes in the treatment of Acne vulgaris. In conclusion, it was discovered that the neem oil components with PubChem IDs: ID_610088 (2-(1-adamantyl)-N-methylacetamide), ID_600826 (N-benzyl-2-(2-methyl-5-phenyl-3H-1,3,4-thiadiazol-2-yl)acetamide), and ID_16451547 (N-(3-methoxyphenyl)-2-(1-phenyltetrazol-5-yl)sulfanylpropanamide) have strong affinities for these drug targets and may thus be used as therapeutic agents in the treatment of acne.

Macromycetes Determined in Silifke (Mersin) District

This study was carried out on the macrofungi samples collected from Silifke district of Mersin province. As a result, 122 macromycete species belonging to 91 genera, 48 families, 18 orders, and 7 classes, within Ascomycota and Basidiomycota were determined. Including the previously reported 11 species, a total of 133 macromycete taxa were compiled from the region. The list of the taxa was provided together with their habitats, collection dates, voucher numbers, and the citations for those reported before.

New records and barcode sequence data of wood-inhabiting polypores in Benin with notes on their phylogenetic placements and distribution

Supplementary information:<br />Fig. S1.Maximum likelihood tree with rapid bootstrap values. The species names are followed by voucher or strain number and country of origin. Species names in red are from Benin.<br />Fig. S2. Maximum likelihood (ML) analysis of the genus Microporus with rapid bootstrap values based on the combined ITS-LSU-Tef dataset.Newly generated sequences highlighted in red. The sequence namesare followed by voucher or strain number and country of origin.Table S1. Taxon names and GenBank accession numbers of somesequences used in this study.

Design of digital kWh-Meter to top-up the electric pulse by automatically using Relay Module Based on SMS and Arduino Uno

This study aims to design a digital kWh-meter prototype to top-up the electricity pulse by automatically using relay modules based on Short Message Service (SMS) and Arduino Uno. The utilization of 12 relay modules to substitute the keypad input function in the digital kWh meter is our basic idea in this study. The method we used to replace the keypad input function with the relay module is based on the integration between the circuit path in the keypad board and the relay module as an electric switch that can activate when the relay gets a trigger from the Arduino Uno. In this study, when the user wants to charge the electric pulse, the user will send the voucher number to the GSM SIM900A module via SMS, then it will be processed to the Arduino Uno. Then Arduino Uno will trigger the relay to be activated so that it can automatically fill the voucher number to the digital kWh-meter. This study result is the success of relay modules can substitute the function of keypad input to fill the voucher pulse number to the digital kWh-meter through SMS with the successful voucher number filling up to 98%. The usefulness of the relay module to change the keypad input function on the digital kWh meter is our original idea for this study.

Ethnomedicinal study and in vitro validation of medicinal plants used for treating Jaundice in Zuru emirate of Kebbi State, Nigeria

Abstract Background: For decades, the resident of Zuru emirate have used herbal medicine to treat liver-related diseases including jaundice. Therefore, the present study was designed to investigate and document the herbal medicine used for treating jaundice in Zuru emirate. Method: Oral interviews and questionnaire were used to document information on medicinal plants, medicinal practices, and demographic profiles of respondents. The medicinal plants that were mentioned were collected, identified, and assigned voucher numbers. The names were further authenticated using www.theplantlist.org and theworldfloraonline.org. Thereafter, the methanol stem bark extracts of three of the most frequently mentioned plants were subjected to in vitro bilirubin degradation assay. Result: A total of 46 TMPs Traditional medicine practitioners responded and cited 28 medicinal plants and medicinal practices used to treat jaundice. The most frequently mentioned plants were Erythrina senegalensis (19.6%) followed by Cochlospermum planchonii (13%), and Anogeissus leiocarpus (13%). The herbal remedies were prepared using different parts of the plants as boiled juice or powder and mostly taken as juice with pap or fresh raw cow milk. The in vitro bilirubin degradation assay revealed a time-dependent and dose-dependent bilirubin degradation by Anogeissus leiocarpus (63.05 %), Erythrina senegalensis (46.33%), and Cochlospermum planchonii (27.45%). Conclusion: The present findings revealed the medicinal plants that are used to treat jaundice and the potential jaundice ameliorative effect of these plants may involve bilirubin degradation. Future in vitro and in vivo mechanistic studies should investigate the jaundice ameliorative potency of these plants.

Kop Dağı (Bayburt-Erzurum)’nın makromantar biyoçeşitliliği

The study was mainly based on macrofungi samples collected from Kop Mount within the boundaries of Bayburt and Erzurum province between 2010 and 2011. Together with some previously presented taxa, a total of 88 macrofungi species belonging to 63 genera, 30 families, eight orders within classes Agaricomycetes, Leotiomycetes and Pezizomycetes have been compiled. The list of the determined taxa were presented together with their habitats, substrates, collection localities and personel voucher numbers.

Intraobserver and Interobserver Reliability of the Lenke Classification Among Spine Surgeons

Objective: The objective of this study was to analyze the intraobserver and interobserver reliability of the Lenke classification among spine surgeons from the city of Salvador, Bahia. Methods: Preoperative imaging (front, profile and lateral inclinations) examinations of 20 patients at the Outpatient Clinic of the of Santa Izabel Hospital Orthopedic Department, Salvador, Bahia, who had been diagnosed with adolescent idiopathic scoliosis, were selected to be evaluated by 15 spine surgeons two times at an interval of 30 days, for analysis of the intraobserver and interobserver reliability of the Lenke classification. The project was first submitted for ethical analysis to the Institutional Review Board of the Santa Izabel Hospital - Santa Casa de Misericórdia da Bahia / Prof. Dr. Celso Figueirôa and approved with voucher number 002650/2019. All the participants signed the Informed Consent Form (ICF). Results: Analyzing the concordance using the Kappa index, interobserver reproducibilities of 0.755, 0.525 and 0.840 were obtained for the type of curve and the lumbar and sagittal modifiers, respectively, while the intraobserver reliabilities for the same parameters were 0.921, 0.370 and 0.929. Conclusion: For the study population, the reliability of Lenke’s classification was moderate to almost perfect. Level of evidence III; Interobserver and intraobserver reliability.

Extended distribution of two endemic <I>Crotalaria</I> species to Telangana state, India

Two endemic Crotalaria species viz., Crotalaria sandoorensis Bedd. ex Gamble and Crotalaria vestita Baker of the family Fabaceae have been reported first time from the Telangana state. Nomenclatural citation, detailed description and phenological data along with distribution map, pictorial data and herbarium voucher numbers have been provided here to facilitate easy identification and future references.