A lab-scale rotating drum biological contactor (RDBC) was implemented and accessed concerning abiotic oxygen transfer rate and treatment capacity for domestic sewage. The dissolved oxygen (DO) transfer test showed that the rotational speed and the submergence level of drum greatly affected the DO in bulk liquid with the volumetric oxygen transfer coefficient KLa of 2.3-25.9 h−1, effectively achieving anaerobic/anoxic/aerobic conditions in the reactor. The post-denitrification process was proposed using the RDBC to degrade the synthetic nitrogen-containing sewage wastewater. When the hydraulic retention time was 16 h, the flow distribution ratio was 3:1 and the anoxic rotational speed was 6 rpm, the effluent NH4+-N and TN were 2.3 mg L−1 and 7.3 mg L−1 with the NH4+-N and TN removal efficiency of 94.1% and 80.9%, respectively. The Nitrosomonas europaea and Candidatus Kuenenia dominated in the anoxic carried-biofilm, indicating that the coexisting of aerobic and anaerobic ammonium oxidation bacteria enhanced the TN loss in the post-anoxic tank. This study provides an innovative non-aeration technology for biological wastewater treatment and has implications to simplify operation and save energy.