The objective of this study was to get more information about the root zone, mainly the gas composition in substrates, which are used in closed hydroponic systems. For analyses of carbon dioxide (CO2), ethylene (C2H4) and oxygen (O2) different methods were proved and used. For analyses of carbon dioxide and ethylene, a gas sampling system was used to get gas samples from the root zone. CO2 gas samples of 20 ml were analyzed with an IR sensor by air diffusion into a measuring cuvette. C2H4 gas samples of 3 ml were analyzed using gas chromatography (GC). Gas sample cells were inserted into the substrates at 2 or 3 heights. Dissolved oxygen was determined with a membrane covered galvanic sensor, in the air, in the in the nutrient solution, and in the drain solution. To analyze the root zone, a hydroponic system with chrysanthemum cv. 'Snow' on 3 substrates was performed. The following substrates were used: Sawagrow (polyester fiber), polyester fleece (thin layer of fleece), and UC-mix (peat substrate). Gas sampling at 2 or 3 heights from root zone did function well, results ranged from 350 ppm to 7700 ppm CO2, and from 0.0088 ppm to 0.1147 ppm of C2H4. The organic substrates UC-mix contains more CO2, from top to the bottom the CO2 level increased. The CO2 level is influenced by microbial and root respiration. The determined CO2 concentration is no limiting growth factor. Results of C2H4 shown an influence by the substrates and a gradient from the top to the bottom. But highest level was determined in the middle and at the bottom of the substrates. The time course shows during the day pattern according the photoperiod. The trend of C2H4 level was almost constant during the experiment. The dissolved O2 level in solution was about 57-82% and in drain solution 78%-99%. O2 level of solution and drain can be an indicator for oxygen deficiency, but it is no direct measurement in root zone or roots. The O2 content in air between 10.5% to 20.4%. During the experiment the O2 concentration decreased; for CO2 the opposite trend was found. INTRODUCTION Plant growth in closed hydroponic systems, with restricted root environment, and consequently, low buffering capacity is more related to water, nutrient solution, and oxygen supply. Water and nutrients are supplied with the nutrient solution, which is regulated by the composition of the solution and irrigation control. Available oxygen is mainly determined by the layout of the hydroponic system and the physical substrate properties. The oxygen diffusion rates into the water depends directly on volumetric air content, the partial oxygen pressure, and temperature. Within the hydroponic systems there is an oxygen gradient for design flow techniques and flow rates (Vestergaard, 1984; Bunt, 1991; Baas et al. 2000; Wever et al., 2000). In addition, the substrate parameters change at the end of each crop, due to decomposition of organic substrates or root and increasing root mass (Wever and van Leeuwen, 1995). Oxygen deficiency may be a limiting factor for plant growth. Oxygen deficiency can be tolerated by roots for a short time, even for several days (Buwalda, 1991; Gislerod et al., 1997; Yosida et al., 1997). Proc. IS on Growing Media Eds.: Alsanius, Jensen & Asp Acta Hort 644, ISHS 2004