Vitex Pinnata Research Articles

Acute Toxicity Assessment of Cream Ethanolic Extract of Vitex pinnata Bark from Central Kalimantan.

Vitex pinnata bark has the ability to scavenge free radicals and has antimicrobial activity, thus accelerating wound healing. The formulation of V. pinnata bark preparations has good organoleptic properties, homogenity, pH, dispersion, and absorption. We studied the acute toxicity of V. pinnata skin cream in Wistar rats according to OECD guidelines 402. Seven nulliparous, non-pregnant female Wistar rats were exposed 2000 mg/kg of body weight. There were no signs of toxicity in any behavioral, necropsy dan histopathological changes. The Globally Harmonized System classified V. pinnata cream extract as category 5 (unclassified) with LD50 more than two thousand mg/kg.

Conservation of the Alaban tree (Vitex pinnata L) in the coastal landscapes at Banyak Island, Aceh Province

Conservation of the Alaban tree (Vitex pinnata L) in the coastal landscapes at Banyak Island, Aceh Province

Metabolic profiling of Vitex Pubescens Vahl bark via UPLC-ESI-QTOF/MS/MS analysis and evaluation of its antioxidant and acetylcholinesterase inhibitory activities

BackgroundAlzheimer’s disease is a neurodegenerative age-related disease that primarily affects the elderly population leading to progressive memory impairments and neural deficits. It is counted as a major cause of geriatric dependency and disability. The pathogenesis of Alzheimer’s disease incidence is complex and involves various hypotheses, including the cholinergic hypothesis, deposition of β-amyloid plaques, neuroinflammation, oxidative stress, and apoptosis. Conventional treatments such as donepezil aim to delay the symptoms but do not affect the progression of the disease and may cause serious side effects like hepatoxicity. The use of natural candidates for Alzheimer’s disease treatment has drawn the attention of many researchers as it offers a multitargeted approach.MethodsThis current study investigates the metabolic profiles of total defatted methanolic extract of Vitex pubescens bark and its polar fractions, viz. ethyl acetate and n-butanol, using ultra-performance liquid chromatography-electrospray ionization-quadrupole time-of-flight tandem mass spectrometry(UPLC-ESI-QTOF/MS/MS) technique as well as evaluate the antioxidant using free radical scavenging assays, viz. DPPH and ABTS assays and in-vitro acetylcholinesterase inhibitory activities using Ellman’s microplate assay.ResultsMetabolic profiling revealed a total of 71, 43, and 55 metabolites tentatively identified in the defatted methanolic extract, ethyl acetate, and n-butanol fractions, respectively. Phenolic acids were the most abundant class, viz. benzoic acids, and acyl quinic acid derivatives followed by flavonoids exemplified mainly by luteolin-C-glycosides and apigenin-C-glycosides. Quantification of the total phenolic and flavonoid contents in the total defatted methanolic extract confirmed its enrichment with phenolics and flavonoids equivalent to 138.61 ± 9.39 µg gallic acid/mg extract and 119.63 ± 4.62 µg rutin/mg extract, respectively. Moreover, the total defatted methanolic extract exhibited promising antioxidant activity confirmed through DPPH and ABTS assays with a 50% inhibitory concentration (IC50) value equivalent to 52.79 ± 2.16 µg/mL and 10.02 ± µg/mL, respectively. The inhibitory activity of acetylcholine esterase (AchE) was assessed using in-vitro Ellman’s colorimetric assay, the total defatted methanolic extract, ethyl acetate, and n-butanol fractions exhibited IC50 values of 52.9, 15.1 and 108.8 µg/mL that they proved the significant inhibition of AchE activity.ConclusionThe results obtained herein unraveled the potential use of the total methanolic extract of Vitex pubescens bark and its polar fractions as natural candidates for controlling Alzheimer’s disease progression.

Harnessing Hyper-Accumulator Plants for an Innovative Green Technology to Recover Nickle from Serpentine Soils in Sri Lanka

Phyto-mining is an environmentally sound green technique that has been employed for metal recovery from low-grade sources using hyperaccumulator plants. Due to its economic viability and wide social acceptance, this technology has been favoured over many conventional mining applications. Nickel (Ni) phyto-mining was introduced to the world with the identification of many Ni hyperaccumulator species grown in Ni-enriched metalliferous soils like serpentine, where they accumulate more than 1,000 mg/kg of Ni. Along the boundary of Highland and Vijayan Complexes in Sri Lanka, Ginigalpelessa, Indikolapelessa, and Ussangoda serpentinite deposits are closely located by showing similar geographical features. According to previous studies, these deposits contain a high concentration of Ni in the soil causing the evolution of serpentinite flora unique to these areas. This study aimed to identify the diversity and the presence of hyperaccumulator species that can be employed in Ni phyto-mining. More importantly, the study focused on identifying annual or biannual crop species with short lifespans that can be used in phyto-mining. The plant diversity and abundance analysis were carried out using a grid sampling method (3×3 m), and the highly abundant species were harvested and subjected to aqua regia digestion to determine hyperaccumulator levels using Inductively Coupled Plasma Mass Spectrometer (ICP-MS). Ginigalpelessa and Indikolapelessa showed similar diversity, abundant with grass and small herb species, such as Apluda mutica, Imperata cylindrica, Eupatorium odoratum, and Tephrosia villosa, where the A. mutica was dominant. In addition, plants like Morinda tinctoria, Vitex pinnata, and Azadirachta indica were dispersed throughout the deposit. Ussangoda deposit exhibited few herb species, including Hybanthus enneaspermus, Evolvulus alsinoides, and Desmodium triflorum, and localized thick vegetation patches. Diversity analysis of all the deposits showed very low plant diversity, ranging from 0.1 to 1.3. Moreover, the areas containing high Ni concentrations (6,000- 10,000 mg/kg) were abundant with stunted trees and grass species. The present study identified the Apluda grass species as a potential Ni hyperaccumulator for phytomining since it accumulates a high concentration of Ni (900-2,800 mg/kg) and can be grown biannually. Even though the grass species was dominant in both Ginigalpelessa and Indikolapelessa deposits, it was not observed in Ussangoda deposit, probably due to the arid climate and the deposition of salt spray from the coast. The findings of this study would be promising to establish phytomining in serpentine soils to support the increasing demand for Ni in the global market, especially for clean energy transition.
 
 Keywords: Low-grade soils, Clean energy transition, Ni phyto-mining, Serpentinite deposits
 
 Financial assistance from SR/LT/2021/15 grant from University of Moratuwa is acknowledged

Antioxidant Activity and Total Flavonoid of Extract of Bark Vitex pinnata L.

Background: The bark of Vitex pinnata L is not yet optimally utilized, primarily being used in boat construction while the remaining material becomes waste that is either buried or burned in trash. Therefore, it is necessary to explore more efficient and economically valuable uses for the bark of the laban tree. Objective: The aim of this research is to identify the chemical compounds, flavonoid content, and antioxidant activity present in the methanol extract of Vitex pinnata L bark. Method: Phytochemical screening was conducted on the methanol extract of Vitex pinnata L. bark. To obtain the extract, the simplicia powder was macerated using methanol as the solvent. The total flavonoid content and antioxidant activity were then tested using the 1,1-diphenyl-2-picryhydrazil (DPPH) method. Results: the phytochemical screening indicated that the Vitex pinnata L. bark extract contains alkaloids, flavonoids, and tannins. The total flavonoid test yielded 17.27%, and the antioxidant activity test revealed that the methanol extract of the laban tree bark has antioxidant activity with an IC50 value of 43.22 ppm. Conclusion: This extract has strong potential as an antioxidant agent.

Alaban wood (Vitex pinnata) as a promising feedstock in the community-scale charcoal industry: A case study of KPHP Kendilo, Indonesia

Indonesian forest production management unit, also called KPHP, have initiated various programs to develop the prosperity of local societies around forest area, including their independence in the energy sector. Charcoal is an ancient green energy which is still traditionally produced by those people for daily usage. Currently, the demand of charcoal for many modern industries in the world has been growing, showing its high potential as future export commodities since Indonesia has a richness in the forest biomass resources for charcoal feedstock. Therefore, we conducted a suitability analysis of the community-scale charcoal production at KPHP Kendilo, one of the forest management units located in East Kalimantan province. Alaban wood (Vitex pinnata) is considered the most potential biomass in this area, indicating its high availability in nature for charcoal feedstok. It was converted by a drum kiln technique using ratio of fuel wood and raw material at 1 : 2 (dry matter) for approximately 4 hours. It showed that the plant having diameter of above 15 cm possessed the highest charcoal yield (6.83 ton/ha), with appropriate energy properties (7,001 kcal/kg). Finally, this study points out the promising of Alaban wood in KPHP Kendilo for charcoal production in the rural communities.

Early Growth of Seven Local Tree Species In The Reclamation Area of PT Bara Tabang

Revegetation of post-mining land with local species has been carried out by coal mining companies in East Kalimantan. However, the information about plant species that can adapt to post-mining land environments is still limited. Therefore, PT Bara Tabang, as a coal mining company, has established a demonstration plot for planting local species in post-coal mining areas. The purpose is to add information on local species that can adapt to the post-mining environment. There were 7 species planted, that is Vitex pinnata, Syzygium polyanthum, Schima wallichii, Shorea balangeran, Melaleuca cajuputi, Syzygium sp., and Hopea mengarawan. 100 plants were planted for each species with the addition of 1 kg of compost for each plant. Observations for six months showed that the survival rate of local species was still above 90%, except for H. mengerawan that only 13%. The species of plants that showed the best growth in height and diameter at 6 months of age were M. leucadendron with 8.08 cm and 0.16 cm respectively. Sc. wallichii showed the lowest growth in height and diameter of 1.60 cm and 0.05 cm respectively at 6 months of age.

Retraction: The Gastroprotective Effect of Vitex pubescens Leaf Extract against Ethanol-Provoked Gastric Mucosal Damage in Sprague-Dawley Rats.

Retraction: The Gastroprotective Effect of Vitex pubescens Leaf Extract against Ethanol-Provoked Gastric Mucosal Damage in Sprague-Dawley Rats.

Characteristics and Antioxidant Activity of Liquid Smoke Produced from Gelam Wood (Melaleuca cajuputi), Alaban Wood (Vitex pubescens Vahl), and Coconut Shells (Cocos nucifera L.)

The antioxidant activity of liquid smoke is a major consideration in its use as a food preservative. Raw materials greatly determine the phenolic content in liquid smoke. The purpose of this study was to determine the physicochemical properties (phenolic & acid content, pH level, and chemical composition) and the antioxidant activity of liquid smokw made from gelam wood (Melaleuca cajuputi), alaban wood (Vitex pubescens Vahl), and coconut shells (Cocos nucifera L). The phenolic content was determined by the Follin-Ciocalteau method, whereas the acid content was determined using the titration method. The antioxidant activity was evaluated using a spectrophotometric method with DPPH radical compounds. The average phenolic contents of LSGW, LSAW, and LSCS were 0.855, 0.885, and 1.957 (% g GAE/mL), respectively, and the average acid contents were 4.256; 2.776 and 11.873 (%g/mL), respectively. Compounds detected in LS consist of organic acids, phenolic compounds, carbonyls, alcohols, furans, and esters. All three types of LS had high antioxidant activity, as indicated by their IC50 values of 0.53% for LSGW, 0.70% for LSAW, and 0.75% for LSCS, respectively. The findings of this study implied that the antioxidant activity of liquid smoke is not always directly proportional to its total phenolic content.

Qualitative Phytochemical Screening, Evaluation of Antioxidant and Anti-Inflammatory Potentials of Vitex pinnata L. Leaf Extract

The purpose of the study was to screen phytochemicals and assess the antioxidant as well as anti-inflammatory properties of Vitex pinnata leaf extracts. Alkaloids, glycosides, tannins, terpenoids, saponins, phlobatannins, steroids, quinine, phenol, coumarins, and flavonoids were found during the phytochemical investigation. V. pinnata leaf extract exhibited an IC50 value of 154.33 µg/ml in the DPPH free radical scavenging assay (standard: IC50 = 25.48 µg/ml) IC50 value of 218.8 µg/ml in the albumin denaturation inhibition assay (standard: IC50 = 33.74 µg/ml). According to the findings, the leaves of V. pinnata contain a variety of pharmaceutically important secondary metabolites capable of scavenging DPPH and inhibiting albumin denaturation, demonstrating their antioxidant and anti-inflammatory properties. J. Bio-Sci. 30(2): 01-11, 2022

Improving Simplicia of Kerangas Forest by Minimizing Microbial Content Under Ultraviolet Radiation Treatment

Kerangas forest is known as a source of traditional medicinal plants and thus potentially the source of simplicia. Medicinal plant preparations of simplicia are usually dried to meet water content standards (< 10%). However, in some cases the drying process carried out still does not eliminate the microbial content in the simplicia. This study aims to analyze the phytochemical compounds contained in three types of medicinal plant simplicia and the total microbial content by treatment with ultraviolet (UV) irradiation. The types of simplicia used were Baeckea frutescens leaves, Shorea balangeran bark and Vitex pubescens leaves (obtained from the kerangas forest). Before testing the total microbial content, simplicia first received UV irradiation. The first simplicia sample was not irradiated, while the next simplicia sample was given UV irradiation with a power of 60 watts and an irradiation time of 1, 2 and 4 h. The microbial content test used the total plate count method with the pour plate analysis technique. S. balangeran is unexposed species to microbes, even without UV irradiation. Radiation with a UV lamp for 1 h with a lamp power of 60 watts and a distance of the sample with a lamp of 30 cm effectively eliminates microbes in B. frutescens simplicia. Radiation for 4 h with a UV lamp of 60 watts and a sample distance of 30 cm was only able to eliminate the microbial content in V. pubescens simplicia. The results of this study indicate that S. balangeran has high potential to be developed as an antibacterial.

INVENTARISASI JENIS VEGETASI POHON DI LABORATORIUM ALAM PENDIDIKAN BIOLOGI SEBAGAI RANCANGAN BUKU SAKU PADA MATERI KEANEKARAGAMAN HAYATI

The types of woody plants in the Nature Laboratory of Biology Education FKIP UNRI have not been completely recorded, besides that the results of observations can be used as a source of learning which is still very rare for teachers to develop pocket books. This study aims to inventory tree vegetation at the Nature Laboratory of Biology Education FKIP UNRI as a pocket book design on biodiversity material. The research was conducted in June-September 2021. This research is survey research and development research. Inventory of tree vegetation types using the roaming method. The development of a pocket book on biodiversity material uses the ADDIE development model. The inventory results show that there are 19 types of tree vegetation in the Biology Education Nature Laboratory, namely Alstonia scholaris. R. Br, Rhodamnia cinereal, Sizygium acuminatissimum, Terminalia catappa. L, Aporasa granularis, Blumeodendron kruzii, Maccaranga gigantean, Endospermum javanicum, Artocarpus elasticus, Ficus carica. L, Artocarpus heterophyllus, Calophyllum inophyllum. L, Vitex pinnata, Switenia macrophylla, Peronema canescens, Symplocos cochinchinensis, Diospyros confertiflora, Schima wallichi. Korth, Cocos nucifera. L. The results of the inventory become a source of pocket book information on biodiversity material.

Prediction of Pharmacokinetic Parameters from Ethanolic Extract Mane Leaves (Vitex pinnata L.) in Geothermal Manifestation of Seulawah Agam Ie-Seu’um, Aceh

The Mane plant (Vitex pinnata L.) is traditionally used as medicine in Aceh Province, Indonesia. This study aimed to predict the pharmacokinetic parameters of compounds in the ethanolic extract of Mane leaf (EEML), including the absorption, distribution, metabolism, excretion, and toxicity (ADMET), by in-silico approach. The method used was to analyze the compounds using a web-predictor server and molecular docking. Gas chromatography-mass spectrometry (GCMS) analysis of EEML showed the presence of active compounds, including phytol (60.93%), acorenol (8.56%), n-hexadecanoic acid (4.89%), trans-Z-alpha-bisabolene epoxide (2.7%) and cedrane (2.03%). Lipinski's rule of five states that all compounds had a deviation of less than 2. Pharmacokinetic parameters suggested that phytol was moderately absorbed in the gastrointestinal tract and had a toxicity level of 5 with lethal doses (LD50) &gt;5000 mg/kg. Molecular docking results showed that phytol could be used against the targeted enzyme Staphylococcus aureus. In conclusion, our study suggests that the active compounds of EEML may have potential as a drug candidate.

POTENSI KESESUAIAN JENIS TANAMAN PADA AREAL LAHAN PASCATAMBANG TIMAH (STUDI KASUS DI KECAMATAN SUNGAI SELAN KABUPATEN BANGKA TENGAH PROVINSI BANGKA BELITUNG)

In addition to providing benefits, mining activities can result in physical, chemical, biological and marginal changes for plant growth. Criteria of plants used for revegetation of land mines are rapidly growing in the neighborhood marginal, allowing other types of grow on the land, producing fruits that are easily spread by birds, and producing a litter easier undergoing decomposition. This research aims to know the biodiversity and natural vegetation types of suitability identification for revegetation land post-mining tin. Observation of post-mining land was carried out to identify the physical and chemical properties of the soil and the type, density, and distribution of vegetation in the observation plots. Analysis of the suitability of vegetation types with rehabilitation land refers to the criteria in Permenhut No P.4/Menhut-II/2011 with modifications. Soil texture on former tin mining land was dominated by sand, is acidic, and has low nutrients. There were 35 types of plants found, ten species of trees and three types of shrubs among them, which have the potential to be used in revegetating post-tin mining land, namely. i.e., the Acacia mangium, Syzygium buxifolium, Syzygium racemosum Blume, Schima wallichii, Trema orientalis, Eugenia sp., Alstonia angustifolia, Ficus padana, Alstonia scholaris, Vitex pinnata L, Syzygium sp, Melastoma malabthricum dan Melastoma polyanthum.

Liver Histopathology of Mice Induced by Paracetamol on Administration of Methanol Extract of Laban Tree Bark (Vitex pubescens Vahl.)

Paracetamol metabolism takes place in the liver mediated by CYP450 enzymes and generates NAPQI radicals. The NAPQI radical is highly electrophilic to hepatocyte biomolecules and is therefore capable of inducing liver damage. Laban tree bark (V. pubescens) methanol extract is known to contain antioxidant compounds of flavonoids, saponins, and tannins with an IC50 value of 19.83 g/ml. This study aims to determine the optimal dose of extract that can improve the liver microanatomy of mice induced by toxic dose of paracetamol (TDP). This study used 30 male Swiss strain mice with a body weight of 20-30 g. The treatments given consisted of negative control (paracetamol 105 mg kg-1), positive control (vitamin E 46.8 mg kg-1), and laban extract at a dose of 75; 150; 300; 600 mg kg-1 BW. Liver microanatomy parameters measured included hepatocyte diameter (19.50±0.21 µm), sinusoids (4.39±0.23 µm), and central vein (36.84 ± 1.79 µm). Data were analyzed using one-way ANOVA and Duncan's Multiple Range Test further test was then presented in the form of mean ± standard deviation. The results showed that extract at a dose of 600 mg kg-1 had hepatocyte, sinusoid, and central vein diameters that were not significantly different (p&gt;0.05) with vitamin E.

Vegetation structure and floristic composition at habitat Rafflesia patma Blume in Pananjung Pangandaran Nature Reserve, West Java, Indonesia

Rafflesia patma Blume is a plant endemic to Pangandaran, West Java, which is protected because of its rare status. This study aims to study the composition of the floristic vegetation that supports the life of R. patma in the Pananjung Pangandaran Nature Reserve, West Java. The research was conducted using a survey method with a purposive sampling technique and sampling using quadrat. The data obtained were then analyzed using the Importance Value Index (IVI), Shannon-Wiener index, and Evenness index. The results showed that R. patma in the Pananjung Pangandaran Nature Reserve lived as a parasite on the roots and stems of Tetrastigma leucostaphylum. The floristic composition of the habitat of R. patma in the Pananjung Pangandaran Nature Reserve, West Java, consisted of 251 individuals divided into 28 species. The plant species with the highest Importance Value Index (IVI) were Syzygium antisepticum, Stephania capitata, Casearia sp., and Buchanania arborescens for the sapling category, as well as Syzygium antisepticum and Vitex pubescens for the tree category. The condition of the vegetation around the habitat of R. patma is quite dense with the diversity index values obtained belonging to the moderate level of diversity. The highest diversity index at the sapling level was found at the Cilegon location with an H' value of 2.14 and the highest diversity index at the tree level was found at the Gua Parat location with an H' value of 1.5.

Analyses of Vegetation Used by Long-tailed Macaque (&lt;i&gt;Macaca fascicularis&lt;/i&gt; Raffles 1821) in Tinjil Island

Tinjil Island is a semi-natural breeding facility for long-tailed macaques (Macaca fascicularis) managed by Primate Research Center, IPB University, located at the southern of Java Island and surrounded by the Indian Ocean. Long-tailed macaques are considered frugivorous even though they are well-known for their flexible diet. This study aims to analyse the vegetation supporting the population of long-tailed macaques. Data were collected from six tracks using square sampling plots with the size of 20 m x 20 m for trees as the main plot, inside the main plot were square subplots consisting of 10 m x 10 m for poles, 5 m x 5 m for saplings, and 2 m x 2 m for seedlings. The Important Value Index (IVI) was calculated for each level of vegetation. Hanjuang (Dracaena elliptica) dominated the seedlings with 29.35%, followed by Kampis (Hernandia peltata) with 18.73%, and Kalapari (Pongamia pinnata) with 13.73%. Hanjuang (Dracaena elliptica) also dominated the saplings with 26.83%, followed by Pancal (Syzygium antisepticum) with 19.19%, and Laban (Vitex pubescens) with 12.30%. The poles were dominated by Ki Cau (Dolichandrone spathacea) as high as 59.28%, while Waru (Thespesia populnea) and Ki Ciat (Ficus septica) dominated at 40.47% and 36.15%, respectively. Kampis (Hernandia peltata) dominated the trees with 39.28%, followed by Ki Ara (Ficus glomerata) with 35.56%, and Ki Langir (Dysoxylum amooroides) with 28.70%. Species found on Tinjil Island are mostly Moraceae (9.84%) and Fabaceae (9.84%), followed by Malvaceae (8.20%), Euphorbiaceae (4.92%), Myrtaceae (4.92%), and Anacardiaceae (4.92%). The vegetation in Tinjil Island supports the livelihood of long-tailed macaques on the island because they have an abundance of food and staple food such as figs to help them fulfil the energy needed to survive and reproduce.

KEANEKARAGAMAN JENIS POHON PENYUSUN CAGAR ALAM PANANJUNG PANGANDARAN

Pananjung Nature Reserve is one of the conservation areas that have unspoiled forest cover. Thus, the ecological processes in the area are still well maintained. This study aims to determine the diversity of vegetation types that make up the Pananjung Nature Reserve. Data were collected using systematic sampling with random start method with nested sampling plots. The sampling intensity used in this study was 0,5% with the number of plots as much as 58. The results showed that there are 59 tree species from 37 families. The highest tree important value index is Vitex pubescens with a value of 29,96%. Meanwhile, the diversity index value (H') of the trees that make up the Pananjung Nature Reserve is 3,55. Based on this value, the diversity index is in high category.

Pemetaan Potensi Biomassa Permukaan Rawan Terbakar Berbasis Citra Landsat 8 Oli Di Kecamatan Kahayan Hilir Kabupaten Pulang Pisau

Peatland fires in 2015 in Central Kalimantan were biomass fires on peatlands that burned with an area of ​​196,987 hectares and one of them was in Kahayan Hilir District. this research aims to determine the potential distribution of surface biomass and identify the types of vegetation. this research uses Landsat 8 OLI imagery in 2020 as well as land cover classification and calculation of the Vegetation Index (NDVI) which is then combined with an overlay to create a map of the distribution of land cover types based on their density which is then used for field checks and surface biomass calculations using the allometric formula on each type of land cover. Based on the research conducted, there are 8 types of land cover that can be identified and the percentage of total biomass, namely high density forest (1,419,013,40 tons or 62.58%), high density shrubs and shrubs (770,449.68 tons or 33.98%). ), High density settlements (44.169.32 tons or 1.95 %), high density oil palm plantations (21,518.77 tons or 0.95 %), High density open land (8,025.37 tons or 0.35%), plantations medium density palm oil (2,690.09 tons or 0.12%), medium density open land (1,545.81 tons or 0.07%) and low density open land (79.65 tons or 0.004%). The types of tree-level vegetation found in high-density forests are laban (Vitex pinnata), mahang damar (Macaranga triloba), and bungur (Lagerstroemia speciosa), while on understorey grass species rija-rija (Scleria sumatrensis) are found

Antioxidant Activity-Guided Isolation of Phenolic Compounds from Leaves of Vitex pinnata (Lamiaceae)

The aim of this study is to isolate phenolic compounds from the ethyl acetate fraction of the leaves of V. pinnata Linn, which have antioxidant properties, guided by a TLC-Bioautography (Thin-layer chromatography) with a reagent of 2,2-diphenyl-1-picrylhydrazyl radical (DPPH). The TLC-antioxidant bioautography technique has several advantages. It does not require a long time in testing, the sample used is small, and it can potentially obtain promising antioxidant-positive compounds.The isolation process led to obtaining two phenolic compounds. The UV-Vis, FTIR, and NMR spectrum analysis showed that the compounds were (1) p-hydroxybenzoate and (2) luteolin. Further antioxidant evaluation by DPPH assay showed a potential radical scavenging DPPH of luteolin (2) with an IC50 of 1.56±0.18 µg/mL.