The article is devoted to the study of the psychomotor abilities of future police officers, taking into account the gender aspect (using the example of cadets of the Dnipropetrovsk State University of Internal Affairs). At the current stage, the issue of modern ways of integrating the gender perspective in the police occupies considerable attention of specialists, namely in the context of bringing it into line with the requirements of international standards. During the performance of official tasks, women and men have equal opportunities in interaction, cooperation, equal distribution of responsibilities, readiness to help regardless of gender, gender self-identification, etc. The goal is to study the features of psychomotor abilities of future police officers of both sexes of the Dnipropetrovsk State University of Internal Affairs. Cadets of both genders of the Dnipropetrovsk State University of Internal Affairs aged 17 to 20 participated in the research (girls n=16, boys n=26). Methods: system-functional analysis; pedagogical experiment; methods of mathematical statistics. Psychophysiological indicators were studied in cadets using the «Diagnost-1» computer method. Mathematical and statistical analysis of the obtained results was carried out using the Statistica 6.0 program. In the course of the experiment, the psychomotor abilities of cadets of both sexes were investigated (maximum muscle effort, the error of differentiated muscle effort (50% of the maximum dynamometry of the hand), the assessment of the sense of time (5 s and 10 s). Significant differences between the group of girls and boys were found only in the indicator of the maximum strength of the hand of the leading hand (p<0.05).Other investigated indicators (accuracy of differentiation of muscle effort and assessment of the sense of time 5 s and 10 s), taking into account the gender aspect, did not show probable differences between those who were studied (p>0.05). The functional mobility of nervous processes is statistically better in boys compared to girls (p<0.05), the results of simple and complex visual-motor tests are statistically better in girls (p<0.05). The results of the strength of nervous processes in young men are significantly better than in girls. Thus, it can be stated that a number of indicators of psychophysiological functions vary in both boys and girls, and according to individual indicators of psychological functions and levels of reactions, they are at the same level (time speed of assessing the situation and quick decision-making). The obtained data make it possible to assert that future police officers, regardless of gender, can perform their professional duties at a high professional level.