Extract Viscosity Research Articles

Investigation of bioactive phytochemical compounds of the Ethiopian medicinal plant using GC-MS and FTIR

Medicinal plants Highly aromatic crude materials are utilized for treating warts as an alternative medicine to surgical treatment because they can be permanently removed from the body. Thus, this investigation aimed to extract plant material from Calotropis procera leaves, describe the phytochemical screening, analyze anti-microbial activities, determine the functional groups in FTIR, and identify the chemical compounds in GC-MS. The PH, specific gravity, and viscosity of the crude extracts of Calotropis procera were determined at 4.5, 0.79, and 0.49, respectively. Analyze the solubility of crude extracts; ethanol can dissolve while water does not. Flavonoids, alkaloids, phenols, tannins, and saponins were also present in the phytochemical screening tests of the Calotropis procera extracts, triterpenoids, terpenoids, and steroids were not present in the crude extract. Flavonoids, alkaloids, phenols, tannins, and saponins are the primary phytochemical components found in therapeutic plant material. The Calotropis procera crude extracts analyzed for functional groups by FT-IR contained a hydroxyl group, alkane, carbonyl, aldehyde, ketone, phenols, ester, alcohol, and methylene. The chemical compounds analyzed by GC-MS of Calotropis procera crude material were found to have 22 main compounds. Of 22 compounds, 5 compounds are active ingredients for the applications of medical purposes. The bioactive compounds found in the Calotropis procera plant extract are neophytadiene, hexahydrofarnesyl, lanosterol, 2,4-dimethylbenzo [H]quinolone, and squalene. Those bioactive compounds have anti-bacterial, analgesic, antipyretic, anti-inflammatory, antimicrobial, antioxidant, antiviral, and anti-cancer properties. In an in vitro antimicrobial activity test, the crude extract effectively inhibited more gram-positive bacteria than gram-negative bacteria. This collective reason is why the traditional therapist uses this Calotropis procera plant for the treatment of warts.

Breeding assessment of winter rye population GC-494/15vv with high viscosity of water extract of grain meal

Breeding assessment of winter rye population GC-494/15vv with high viscosity of water extract of grain meal

Screening of West Cameroonian edible plant-based mucilage for the production of improved protein-polysaccharide conjugates

In developing countries, blending the powders of locally utilized edible plants for mucilage extraction can be a way to address the growing demand for more viscous and soluble cost-effective hydrocolloids. This study aimed to identify mucilaginous edible plants exploited in the western region of Cameroon in order to enhance the viscosity and water solubility of their mucilages. A semi-administered questionnaire facilitated a comprehensive ethno-nutritional survey. Powders (200 μm) from mucilaginous plants available were defatted, oven dried, and used to produce crude extracts in a water bath (50 °C, 1 h, 2 cycles), with distilled water (1:10, m/v), followed by triple filtration (60 μm, 25 °C) and double centrifugation (2900 g, 20 min). The apparent viscosity of these crude extracts was recorded (spindle 2#, 600 s−1, 60 rpm) at 25 °C, and compared to a 0.2% (m/v) xanthan gum solution (in distilled water). The best plant powders were blended (5 blends) for use under an alkaline condition (pH 10, 0.025 M) for optimal liquid mucilage production and its corresponding powder named hibisanua, derived by coagulating the liquid mucilage (4 °C, 96% ethyl alcohol), oven drying (45 °C), powdering, and sieving (200 μm). Some rheological parameters were determined. FTIR and SEM analysis were carried out with the mucilage hibisanua. Out of 300 surveyed participants, 120 (40%) from 17 ethnicities use the identified plants. Thirty species were found, spanning 27 genera and 19 botanical families. Hibiscus esculentus (okra) pods, Triumfetta annua (nkui) bark, and Corchorus olitorius (lalo) leaves are commonly used in traditional food processing. However, only okra (9.23 Pa s) and nkui (5.54 Pa s) liquid mucilage viscosities were similar to xanthan, making their defatted powders interesting. The validated plant powder mixture (F2) thus comprised 10% nkui and 90% okra (w/w). Their crude extract of concentration 1.51% (w/v) showed the highest dynamic viscosity (23.05 Pa s), not significantly different (p ≥ 0.05) from the control. The resulting dry mucilage with a yield of 215.8 mg/g F2 had the highest solubility (83.05%) with a specific viscosity of 11.519 Pa s upon a desirability of 0.73. All polysaccharides’ solutions exhibited pseudoplastic characteristics and shear-thinning behaviour. A concentrated solution (1.51%) of hibisanua had a rheologic index (0.742) and viscous relaxation time (69.25 s) comparable to xanthan gum. Its structural analysis revealed functional group and morphological changes, typical of highly physical gelling pectic mucilages. These findings support the formulated plant powder approach and the use of its mucilage in the food and pharmaceutical industries.

Recovering rhamnogalacturonan-I pectin from sugar beet pulp using a sequential ultrasound and microwave-assisted extraction: Study on extraction optimization and membrane purification

This research focuses on the integrated recovery of rhamnogalacturonan-I (RG-I) pectin from sugar beet pulp (SBP). First, the extraction of RG-I pectin through sequential ultrasound-assisted extraction (UAE) and microwave-assisted extraction (MAE) was assessed. Optimization using a response surface methodology identified the optimal conditions as initial pH 4, 10 min of UAE, and 157 °C for MAE, achieving a 66.0 % recovery of pectooligosaccharides (POS). Additionally, purification through continuous diafiltration and concentration via ultrafiltration of the POS using membranes with different molecular weight cut-offs (MWCO) was explored. In contrast to previous research using discontinuous diafiltration, the use of continuous diafiltration allowed a decrease in the extract viscosity and obtained higher yields using a higher MWCO membrane. The refined RG-I pectin solids exhibited a high global yield (39–40 g pectin/100 g SBP), and high-methoxyl characteristics, as well as purity levels (70–80 %) similar to commercial prebiotics.

Acoustic droplet ejection facilitates cell-based high-throughput screenings using natural products

Natural Products (NPs) are one of the main sources for drug discovery. Many clinical drugs are NPs or NP-inspired compounds, and recently discovered New Chemical Entities (NCEs) of NPs are emerging as promising new drugs. High-Throughput Screening (HTS) of large sample sets or libraries has grown to be vital for the drug discovery field. Industrial-scale HTS of NP libraries can be limited due to the difficulties entailed in working with tiny extract volumes and the variability in viscosity of NP extracts. For these reasons, the implementation of new technologies to miniaturize different reagent volumes grows to be fundamental. Since Acoustic Droplet Ejection (ADE) emerged as a helpful tool in HTS campaigns for the transference of compound libraries. The aim of this work was to test the effectiveness of ADE for the dispensation of NP extract libraries in cell-based HTS assays.

Analysis of nucleotide sequences of the <i>GT47</i> glycosyltransferase gene in rye cultivars differing in the content of water-soluble pentosans in grain

Rye (Secale cereale L.) is the most important crop in Russia, its grain quality depends on the content of water-soluble pentosans. The grain of rye cultivars with high content of water-soluble pentosans has good baking properties, but low fodder qualities. Methods of marker-assisted selection for this trait in rye remain undeveloped. For Triticeae, the content of pentosans in grain may be associated with the GT47 glycosyltransferase genes, but the genes of this family have not been identified in rye. The aim of this study was amplification, sequencing, and search for single nucleotide substitutions or other mutations in the GT47 gene in various rye cultivars differing in the content of water-soluble pentosans in their grain and the viscosity of their aqueous extract. DNA from rye leaves was isolated by the standard CTAB method. Based on the nucleotide sequences of the bread wheat and barley GT47 genes, universal primers were selected, then a fragment of the open reading frames of the studied gene was amplified, and the nucleotide sequences were determined by automatic capillary sequencing. The population cultivars of rye, ‘Chulpan 7’ and ‘Podarok’, and F1 hybrids ‘KVS Aviator’, ‘KVS Magnifico’ and ‘KVS Eterno’ were analyzed. The highest content of pentosans and the highest kinematic viscosity of the aqueous extract were found in cv. ‘Chulpan 7’. The lowest values of these indicators were shown by the hybrid cv. ‘KVS Aviator’. The analysis of the nucleotide sequences of the GT47 gene revealed the presence of single-nucleotide substitutions in seven loci, in which the studied rye cultivars differed. Of these, the high-pentosan cv. ‘Chulpan 7’ and the low-pentosan cv, ‘KVS Aviator’ differed in three nucleotide substitutions: 159 (G/A), 204 (C/T), and 327 (G/A). It is suggested that these SNPs can be used for genotyping rye cultivars for the content of water-soluble pentosans in grain.

The effect of various sweeteners on the physical, chemical, and organoleptic characteristics of ginger leaf extract syrup

The ginger leaf is an underutilized component of the ginger plant that contains antioxidant chemicals in the form of terpenoids, phenols, and flavonoids, making it effective as a free radical inhibitor. To improve the advantages of ginger leaves, it may be utilized as a food supplement product in the form of syrup as an alternative in product development. The purpose of this study was to determine the effect of sweeteners on the physical properties (viscosity), chemical properties (pH, total phenol, and antioxidant activity), and organoleptic properties of ginger leaf extract syrup (SEDJ), as well as the optimal type of sweetener to generate the most favoured syrup. With five repeats, this study employed a range of sweeteners, including T1 (sorbitol), T2 (sucrose), T3 (HFS), and T4 (honey). The results revealed that sweetener usage in SEDJ had a significant influence (p<0.05) on the pH, viscosity, and organoleptic properties of ginger leaf extract. Based on the highest level of panellist preference, SEDJ with honey sweetener is the best treatment that is nutritious as an antioxidant.

Comparative assessment of the winter grain crops’ feeding value by the content of arabinoxylans

ABSTRACT The main difference in fodder qualities of winter rye, triticale and wheat grains is the content of anti-nutritive substances: water-soluble pentosans and, accordingly, the viscosity of the aqueous extract of grain. Lack of scientific information about the content of antinutrients in the grain of winter cereal crops determines the relevance of the research; and the possibility to develop ways of using it and volumes of production for fodder purposes determines study’s innovativeness. The paper was set out to make a comparative assessment of the winter rye, winter triticale and winter wheat grain quality. On average for three years, the protein content of winter wheat grain was 11.5%, winter triticale – 10.0% and winter rye – 8.5%. In terms of starch content, winter wheat grain differs significantly from winter rye grain (by 0.9 percentage points more); winter triticale (by 0.8 percentage points more). On average, over three years, phosphorus in winter rye grain was 0.02 percentage points less than in wheat grain and 0.03 percentage points less than in triticale grain. Winter triticale and wheat grains hardly vary in the content of arabinoxylans and the viscosity of the aqueous extract. The results can be used in fodder production and animal husbandry.

ПЕРСПЕКТИВЫ ИСПОЛЬЗОВАНИЯ И ПРИЕМЫ СНИЖЕНИЯ АРАБИНОКСИЛАНОВ КОРМОВОГО ЗЕРНА ОЗИМОЙ РЖИ

The purpose of the research was to establish the nature and degree of influence of the methods of preparing the grain of winter rye for feeding and on the content of antinutrients in it. To achieve the goal of the research, laboratory experiments, laboratory analysis of the content of arabinoxylans in the grain by the orcinol-chloride method Albaum H.G. and Umbreit W.W. modified Hashimoto S. and the kinematic viscosity of the aqueous grain extract with the capillary viscometer VPZH-1 were carried out. Grain for laboratory analysis was grown in the Educational Scientific Center of the Bashkir GAU, located in the southern foreststeppe zone of the Republic of Bashkortostan. The results of studies of methods for reducing arabinoxylans in the grain of winter rye are presented. Extrusion, treatment with an ultrahigh frequency electromagnetic field (1.61 and 2.44 times, respectively) and enzyme preparations Fidbest-W and Cellolux-F significantly reduce the content of arabinoxylans and the viscosity of the aqueous grain extract. As a result of extrusion treatment, the content of arabinoxylans in the grain of winter rye decreased by 1.34 times, the kinematic viscosity of the aqueous extract – by 2.42 times. Extrusion of rye grain causes the breakdown of starch and a significant increase in soluble carbohydrates (by 19.9 percentage points). Grain treatment with microwaves (power 700 W and exposure 180 seconds) reduced the content of arabinoxylans by 1.44 percentage points and the kinematic viscosity of the aqueous extract by 2.44 times. Treatment with enzyme preparations Fidbest-W and CelloluxF with a norm of 100 mg/kg led to a decrease in the kinematic viscosity of the aqueous extract of winter rye grain by 9.8 and 7.3 times.

Productivity and nutritional qualities of grain of new F1 winter rye hybrids in the Southern Ural

Abstract The paper presents field experiments and laboratory analyses to study productivity and nutritional qualities of the grain of four F1 hybrids of winter rye (Aviator, Ravo, Prommo, Eterno), and the population variety Chulpan 7 in the natural conditions of the Southern Ural. The research revealed an individual difference in the nutritional qualities of grain and the content of anti-nutritional substances between different studied F1 hybrids, and compared them with the Chulpan 7 variety. Hybrids of winter rye formed higher grain productivity (from 4.65 t/ha to 6.07 t/ha) in comparison with the Chulpan 7 variety. The paper assesses the content of anti-nutritional substances (water-soluble pentosans) in grain, the kinematic viscosity of the water-extractable from grain hybrids (water-soluble pentosans 1.55 ‒ 1.74%, the viscosity of the water extract 18.3 ‒ 23.1 cSt and winter rye variety (of water-soluble pentosans 1.75%, the viscosity of the water extract was 23.4 cSt), and the dependence of these indicators on the environment during the growing season. The paper also presents the results of determining the nutritional value of the grain of hybrids and the Chulpan 7 variety by the content of crude proteins, starch, phosphorus, calcium, and potassium.

Natural deep eutectic solvent as tool for improving Rosa canina L. polyphenol recovery in maceration

Rosa canina L. extracts were prepared using water or three natural deep eutectic solvents (NADESs: betaine+malic acid, betaine+sucrose, and citric acid+sucrose with 50% of water) and maceration. The extracts were characterized in terms of total polyphenol content (TPC), ABTS radical scavenging potential, extraction yield, zeta potential, conductivity, pH, density, surface tension, and viscosity. TPC was the highest in betaine+malic acid extract (10.4 mg gallic acid equivalents, GAE/g), and the lowest in water and citric acid+sucrose extracts (6.5 and 6.4 mg GAE/g, respectively). ABTS radical scavenging potential was the highest in water extract, 5.6 mmol Trolox/g, whereas the lowest was in citric acid+sucrose extract, 2.6 mmol Trolox/g. Extraction yield was the lowest for betaine+malic acid extract, 0.607 %, and statistically significantly higher for betaine+sucrose extract, 1.22 %. Zeta potential (absolute value) was the highest for betaine+sucrose extract (-2.12 mV), and the lowest for citric acid+sucrose extract (0.29 mV). Conductivity was in the range of 0.25 mS/cm (betaine+sucrose extract) to 5.46 mS/cm (betaine+malic acid extract). pH ranged from 3.0 in betaine+malic acid extract to 4.5 in water and betaine+sucrose extracts. Density varied from 1.00 g/mL for water extract to 1.19 g/mL for betaine+sucrose extract, while surface tension varied from 35.0 mN/m for betaine+sucrose extract to 40.6 mN/m for water extract. Viscosity of water extract was 1.52 mPa·s and it was significantly higher for citric acid+sucrose extract, 10.67 mPa·s. The application of NADESs as an extraction medium can improve polyphenol recovery from rose hips, as well as extraction yield and conductivity, but depending on NADES composition. Namely, the highest TPC and conductivity were measured in betaine+malic acid extract, while betaine+sucrose extract possesses the highest extraction yield. Thus, the constitution of NADES should be optimized depending on the future application of the extract.

Variability of selected quantitative traits in new spring barley genotypes

The research included 19 breeding lines and 4 cultivars of spring barley from the preliminary field experiments harvested in 2020 in Radzików. All barley samples were characterized for the content of protein, non-starch polysaccharides (NSP) with soluble (S-NSP) and insoluble (I-NSP) fractions and β-glucan. Additionally, viscosity of water extracts (WEV) was measured to determine the functional properties of the grain. It was the most diverse parameter (CV = 27%) and was significantly correlated with β-glucan content (r = 0.50; for p <0.05). This dependence is shown by the results obtained for the grain of the Avatar cultivar and the RAH 744/19 breeding line, in which the content of β-glucan (5.3% and 4.8%, respectively), as well as the WEV (3.3 mPa.s and 3.0 mPa.s, respectively) were the highest. The lowest content of β – glucan (3.5%) and one of the lowest WEV values (1.4 mPa.s) were observed for KWS Jessie cultivar. Principal component analysis (PCA) showed a substantial impact of the two components PC1 and PC2 on the variability of the analyzed material showing significant variability of the 5 barley genotypes and confirmed the previous results of biochemical analyzes. Our results made it possible to indicate several genotypes that may constitute a source of variability in breeding works aimed at improving the quality of barley. Presented study also show that the grain of some new barley genotypes, with a favorable chemical composition from a fodder and brewing perspective, is a good material for future use in industry.

Identification of genes involved in the formation of soluble dietary fiber in winter rye grain and their expression in cultivars with different viscosities of wholemeal water extract

The grain of rye (Secale cereale L.) used for baking contains a large amount of non-starch polysaccharides, making it an excellent component of functional foods. But rye grain intended for alcohol production and forage use should have a reduced content of these polysaccharides. A comprehensive parameter that can predict the best field of application for winter rye grain is the viscosity of its wholemeal water extract. However, our understanding of the genetic background underlying this key trait and associated features of rye grain is poor. By analyzing six Russian winter rye cultivars, we identified the most contrasting forms and characterized the peculiarities of their water-soluble carbohydrates capable of influencing the viscosity of water extracts. Then, using phylogenetic and transcriptomic analyses, we identified in the rye genome many genes encoding putative glycosyltransferases and glycosylhydrolases responsible for the synthesis and degradation of arabinoxylans, mixed-linkage glucans, cellulose, and some other polysaccharides. We determined the dynamics of mRNA abundance for these genes at three stages of kernel development. Comparisons of gene expression levels in two contrasting cultivars revealed specific members of multigene families that may serve as promising targets for manipulating non-starch polysaccharide content in rye grain. High-viscosity cultivars were characterized by up-regulation of many glycosyltransferases involved in the biosynthesis of arabinoxylans and other cell-wall polysaccharides, whereas low-viscosity cultivars showed up-regulation of several genes encoding polysaccharide-degrading enzymes.

Implementation of a Density Sensitive Structure in the Torsionally Oscillating Resonant Pipe Viscosity Sensor

We present an improved version of a torsionally oscillating resonant pipe viscosity sensor. The new design offers the possibility to sense both, viscosity and density independently and at the same time. The first version of the sensor allowed only sensing the viscosity-density product, where the two values were not separable without additional knowledge or measurements. For the new design, density sensitive structures were additionally implemented into the pipe system. These structures force the fluid to follow the torsional oscillation of the pipe, therefore, in this region the fluid influences the behavior of the resonant structure like an additional mass. This leads to a frequency shift which is mainly density dependent. Due to this behavior it is now possible to use a higher order model to extract both viscosity and density out of one single measurement. We present the newly introduced sensor design, compare it to the first sensor design, describe the additional density sensitive structures and explain how the sensor was designed and evaluated using the Finite Element Method (FEM). We show how different values of viscosity and density influence the resonance frequency and the damping characteristics of the sensor. To prove the concept, we apply a calibrated higher order model to the simulation results and provide a first accuracy estimation.

Evaluation of dry-cured ham pastiness through rheological measurements of aqueous extracts obtained in vitro mimicking the mastication process

Pastiness is a dry-cured ham defect that can be determined by sensory and chemical analysis. Since pastiness produces an increase of saliva viscosity, rheology might be useful to instrumentally evaluate this defect. The aim of this study was to evaluate the rheological behaviour of water extracts obtained in vitro mimicking the mastication of dry-cured ham samples with different pastiness intensities. Commercial samples from different groups (non-pastiness (NPG), medium pastiness (MPG) and high pastiness (HPG) groups) were sensorially and rheologically analysed. Effect of temperatures and HPP treatments at 7 °C, 20 °C and 35 °C were also evaluated. Results showed that apparent viscosity of the water extracts increased with the increase of pastiness and decreased with temperature. Significant differences (p < 0.05) were observed between NPG/MPG and HPG samples and between 25 °C and 37 °C in HPG. After HPP, sensory pastiness disappeared in samples from MPG at any temperature and decreased from 6.8 to 0.2 in samples from HPG only when HPP35 was used. Although the increase on viscosity of oral fluid is related to the sensory pastiness, other factors seem to be involved in the mechanism of pastiness perception. Despite this, HPG samples can be distinguished from MPG and NPG samples.

Changing Wheat Bran Structural Properties by Extrusion-Cooking on a Pilot and Industrial Scale: A Comparative Study.

Extrusion-cooking can be used to change the techno–functional and nutrition-related properties of wheat bran. In this study, pilot-scale (BC21) and industrial-scale (BC45) twin-screw extrusion-cooking using different types of extrusion (single-pass, double-pass and acid extrusion-cooking) and process parameters (temperature, moisture) were compared for their impact on wheat bran. When applying the same process settings, the higher strong water-binding capacity, extract viscosity and extractability displayed by bran extruded using the industrial set-up reflected a more considerable wheat bran structure degradation compared to pilot-scale extrusion-cooking. This was attributed to the overall higher specific mechanical energy (SME), pressure and product temperature that were reached inside the industrial extruder. When changing the type of extrusion-cooking from single-pass to double-pass and acid extrusion-cooking, wheat bran physicochemical characteristics evolved in the same direction, irrespective of extruder scale. The differences in bran characteristics were, however, smaller on industrial-scale. Results show that the differentiating power of the latter can be increased by decreasing the moisture content and increasing product temperature, beyond what is possible in the pilot-scale extruder. This was confirmed by using a BC72 industrial-scale extruder at low moisture content. In conclusion, the extruder scale mainly determines the SME that can be reached and determines the potential to modify wheat bran.

In vitro studies to characterise different physico-chemical properties of some feed grains and their impact in monogastric nutrition

Characterisation of the variations in physico-chemical properties of grains may help to improve the feeding value of grains for animal nutrition. Thus, this study aimed to obtain more extensive quantitative ideas concerning different physico-chemical properties of wheat, hybrid rye, and barley. The samples were ground in a hammer mill using screen size of 1, 3, and 6 mm, respectively. The cumulative mean particle distribution at >1.0 mm of the ground grains showed significant differences between wheat and hybrid rye (4.63 and 9.13%, respectively). At dry sieve analysis of 6 mm screen size, hybrid rye had significantly higher mean particle size distribution of >1.0 mm (26.8%) than for ground wheat and barley. Ground wheat using a 1 mm mesh sieve had the lowest water holding capacity and swelling capacity (1.89 g H2O/g dry matter (DM); p = .001 and 1.33 mL H2O/g DM; p = .021, respectively) compared to hybrid rye and barley. Ground hybrid rye using a 1 mm mesh sieve had the significantly highest extract viscosity (6.22 mPa s). Ground wheat had the lowest (p < .001) corrected sediment rate. In general, ground hybrid rye had always a higher feed particle size >1 mm regardless of the grinding size. Ground wheat had the lowest water holding capacity irrespective of the grinding mesh sieve. Finally, hybrid rye in general is characterised by high extract viscosity (6.22 mPa s at 1 mm grinding size), which decreased with coarser grinding (3.75 and 3.10 mPa s at 3 and 6 mm, respectively). Highlights Particle size distribution is directly affected by the grinding process. Water holding capacity and swelling capacity are two complementary measurements. Extract viscosity seems to be affected by grinding; however, the sedimentation rate is influenced by the grain type.

Characterisation of Wheat–Oat Flour Extrudate: Physicochemical and Β-Glucan Attributes

The objective of this study was to evaluate the effect of wheat–oat flour ratio on the physical properties and β-glucan characteristics of extrudates. Results showed that increasing the wheat–oat flour ratio resulted in a decrease in the water solubility index (r2=0.8567) and hardness (r2=0.9316), whereas the expansion ratio (r2=0.9307) and water absorption index (r2=0.9061) increased. Wheat flour generally caused an increase in L values from 57.81 to 62.94 providing bright samples. Few cells were observed at high wheat–oat flour ratios under a scanning electron microscope, and a smooth surface was noted. Meanwhile, the total (r2=0.9867) and soluble (r2=0.9848) β-glucan contents were inversely proportional to the wheat–oat flour ratio. Extrudates with added wheat flour had a high molecular weight, but wheat flour had no significant (P&lt;0.05) effect on the viscosity of β-glucan extracts. Conclusively, incorporation of wheat flour at a wheat–oat flour ratio of 2.33 provides ready-to-eat food based on whole oat flour, on account of improving the texture and providing sufficient β-glucan contents (0.806 g/100 g) without significantly affecting β-glucan viscosity.

Single-pass, double-pass and acid twin-screw extrusion-cooking impact physicochemical and nutrition-related properties of wheat bran

The potential of extrusion-cooking as a technique to change the physicochemical characteristics of wheat bran, and thereby its nutrition-related properties was investigated. It was investigated whether double-pass extrusion-cooking or the addition of citric acid had a larger effect than single-pass extrusion-cooking alone. Double-pass extrusion-cooking resulted in the highest strong water-binding capacity (1.37 g/g dm), while acid extrusion-cooking resulted in the lowest (0.66 g/g dm) due to hydrolysis of bran polymers that contribute to the microstructure. Double-pass and acid extrusion-cooking increased the amount of water-extractable arabinoxylan, the main dietary fiber constituent (from 0.62 % to 1.75 % and 3.11 % dm, respectively), and therefore also increased extract viscosity. An increased in vitro fermentation rate was observed for double-pass compared to single-pass extruded bran. More phytate was degraded and more ferulic acid released during double-pass extrusion-cooking as compared to acid and single-pass extrusion-cooking. In conclusion, double-pass and acid extrusion-cooking distinctly modify wheat bran physicochemical and nutrition-related properties.

Research of water extraction from xenoderm as an active pharmaceutical ingredient in drugs

Today, burns are one of the most common types of injuries in the home and at work around the world. Therefore, the issue of treatment of burns remains relevant today for medicine and pharmacy in particular.&#x0D; In Ukraine, the method of treatment of burns using xenodermoimplants from porcine skin is used, and therefore the crushed substrate of cryolyophilized porcine skin (xenoderm) is a promising active ingredient in the technology of various drug forms.&#x0D; The aim of the work was to study the structural and mechanical properties of water extract from the crushed substrate of the xenoderm and to determine its amino acid composition by using physicochemical analysis, namely using high performance liquid chromatography (HPLC).&#x0D; A glass pycnometer and a Heppler BH 2 MLW drop ball viscometer were used to determine the density and viscosity of the water extract from the xenoderm. The density and viscosity of the water extract were studied at different temperatures.&#x0D; The dependence of the density and viscosity of the water extract from the xenoderm on temperature was studied and it was found that with increasing temperature the dynamic viscosity decreases and the density changes slightly.&#x0D; A glass pycnometer and a viscometer with falling ball were used to determine the density and viscosity of the xenoderm water extract. Chromatographic separation of amino acids was performed on a liquid chromatograph Agilent 1200 with a fluorescent detector.&#x0D; Chromatographic determination of amino acids was performed on a liquid chromatograph Agilent 1200 (USA) with a fluorescent detector G1315A (USA) and an autosampler 1313A.&#x0D; Using the HPLC method, 16 amino acids were identified (essential – 6; conditionally – 2; nonessential – 8). Identified amino acids are almost in a bound state (1.5%), the largest amount is glutamic acid (0.23%), glycine (0.19%), aspartic acid (0.18%), proline (0.17%) and arginine (0.17%). In unbound form, the content of glutamic acid (0.09%) and glycine (0.06%) is the highest.&#x0D; Based on the results of research, you can choose quality indicators, to determine the appropriate criteria that can be proposed for the standardization of water extract from the crushed substrate of the xenoderm.