Visceral Responses Research Articles

Subjective visual sensitivity in neurotypical adults: insights from a magnetic resonance spectroscopy study.

Altered subjective visual sensitivity manifests as feelings of discomfort or overload elicited by intense and irritative visual stimuli. This can result in a host of visual aberrations including visual distortions, elementary visual hallucinations and visceral responses like dizziness and nausea, collectively referred to as "pattern glare." Current knowledge of the underlying neural mechanisms has focused on overall excitability of the visual cortex, but the individual contribution of excitatory and inhibitory systems has not yet been quantified. In this study, we focus on the role of glutamate and γ-aminobutyric acid (GABA) as potential mediators of individual differences in subjective visual sensitivity, measured by a computerized Pattern Glare Test-a series of monochromatic square-wave gratings with three different spatial frequencies, while controlling for psychological variables related to sensory sensitivity with multiple questionnaires. Resting neurotransmitter concentrations in primary visual cortex (V1) and right anterior insula were studied in 160 healthy participants using magnetic resonance spectroscopy. Data showed significant differences in the perception of visual distortions (VD) and comfort scores between men and women, with women generally reporting more VD, and therefore the modulatory effect of sex was considered in a further examination. A general linear model analysis showed a negative effect of occipital glutamate on a number of reported visual distortions, but also a significant role of several background psychological traits. When assessing comfort scores in women, an important intervening variable was the menstrual cycle. Our findings do not support that baseline neurotransmitter levels have a significant role in overreactivity to aversive stimuli in neurotypical population. However, we demonstrated that biological sex can have a significant impact on subjective responses. Based on this additional finding, we suggest that future studies investigate aversive visual stimuli while examining the role of biological sex.

Nepali Translation, Validity and Reliability Study of the Cohen-Hoberman Inventory of Physical Symptoms for Utilization With Bhutanese Refugees.

Language-appropriate outcome measurements help to improve health equity. The purpose of this study was to translate and validate the Cohen-Hoberman Inventory of Physical Symptoms (CHIPS) in Nepali for Bhutanese refugee utilization. English-Nepali forward and back translations of CHIPS were completed by an official translator and evaluated by three content experts. A scaled rubric measured the following constructs: neurogenic stress response (NSR), somatic stress response (SSR), and visceral stress response (VSR). Data were analyzed using SPSS 26.0. The Nepali version of CHIPS reported good content validity, strong internal consistency (Cronbach's α =.94), and inter-rater reliability (ICC=0.91). Kappa statistic reported 88% to 96% agreement. Constructs of NSR (0.91), SSR (0.94), and VSR (0.94) reported strong internal consistency. The Nepali translated version of CHIPS showed strong validity and reliability for utilization in the Bhutanese refugee population and improves health access to outcome measurements for a vulnerable population.

AESTHETIC ALCHEMY FOR UNVEILING THE TRANSFORMATIVE POWER OF ARTISTIC CHOICES IN POSTCOLONIAL TRAUMA NARRATIVES: EVALUATING THROUGH THE INTERPRETATIONS OF AMITAV GHOSH'S SELECTED NARRATIVES

A crucial role is played by aesthetics in shaping trauma narratives, impacting how individuals perceive, undergo, and process traumatic events. In this context, "aesthetics" encompasses the artistic or stylistic elements employed in storytelling across various mediums like literature, film, visual arts, and more. Aesthetic choices such as symbols and metaphors become potent tools for conveying intricate emotions and experiences linked with trauma, providing a layer of abstraction that facilitates a nuanced exploration of its psychological and emotional dimensions. Aesthetics also influence the structure of a trauma narrative, with elements like flashbacks, non-linear timelines, or fragmented storytelling mimicking the disjointed nature of traumatic memories, effectively conveying the disorientation experienced by those who have undergone trauma. In visual arts and cinema, the visual representation of trauma significantly influences its perception, employing stark imagery, visual metaphors, and symbolic elements to communicate the profound impact of trauma, transcending the limitations of language in capturing its depth. Aesthetic elements like soundtracks and musical scores contribute to the emotional tone, heightening tension, creating a sense of foreboding, and influencing the audience's engagement with the traumatic content. In literature, the choice of language and literary devices shapes the narrative style, impacting how trauma is portrayed. Vivid descriptions, metaphors, and unique linguistic styles evoke a visceral response, fostering a more intimate connection between readers and the trauma narrative. Additionally, aesthetics can influence the resolution of a trauma narrative, utilizing specific visual or narrative elements to contribute to a sense of catharsis or closure for both the characters and the audience, providing a pathway towards healing and recovery. In summary, aesthetics serve as a crucial lens through which trauma narratives are presented and experienced, enabling creators to enhance their depth, emotional resonance, and impact, fostering a greater understanding and empathy for the complex nature of trauma through thoughtful and intentional artistic choices. In the context of Amitav Ghosh's novels, the discussion of postcolonial historic aporia becomes significant, especially given Ghosh's aversion to the term "postcolonial" which tends to portray the aftermath of colonialism in a negative light. Ghosh expresses his dissatisfaction with this characterization, stating, ". . . when I think of the world that I grew up to inhabit, my dominant memory of it is not that it was trying to be a successor state to a colony; it was trying to create its own reality, which today is the reality that we do inhabit" (Kumar 105). This perspective reflects Ghosh's inclination to focus on the efforts of postcolonial societies in shaping their distinct realities rather than being defined solely in opposition to their colonial past. Ghosh's novels, influenced by this viewpoint, illustrate the complexities of postcolonial experiences through the aesthetics of storytelling. Within his narrative framework, he employs symbolism, metaphor, and narrative structure to delve into the intricacies of trauma. Through these aesthetic choices, Ghosh offers a nuanced exploration of the psychological and emotional dimensions of characters' traumatic experiences, challenging conventional postcolonial narratives that tend to emphasize negativity. Ghosh's deliberate use of visual representation, sound, and literary devices in portraying trauma further underscores his commitment to presenting a multifaceted understanding of postcolonial realities. Consequently, his intentional and thoughtful aesthetic choices serve as a critical lens through which postcolonial trauma narratives are depicted, fostering a richer appreciation for the intricate nature of these stories and the diverse realities they represent.

Effectiveness of immune checkpoint inhibitor therapy on bone metastases in non-small-cell lung cancer.

Bone metastases (BoMs) are prevalent in patients with metastatic non-small-cell lung cancer (NSCLC) however, there are limited data detailing how BoMs respond to immune checkpoint inhibitors (ICIs). The purpose of this study was to compare the imaging response to ICIs of BoMs against visceral metastases and to evaluate the effect of BoMs on survival. A retrospective, multicentre cohort study was conducted in patients with NSCLC treated with nivolumab or pembrolizumab in Alberta, Canada from 2015 to 2020. The primary endpoint was the real-world organ specific progression free survival (osPFS) of bone versus visceral metastases. Visceral metastases were categorized as adrenal, brain, liver, lung, lymph node, or other intra-abdominal lesions. The secondary outcome was overall survival (OS) amongst patients with and without BoMs. A total of 573 patients were included of which all patients had visceral metastases and 243 patients (42.4%) had BoMs. High PD-L1 expression was identified in 268 patients (46.8%). No significant difference in osPFS was observed between bone, liver, and intra-abdominal metastases (p=0.20 and p=0.76, respectively), with all showing shorter osPFS than other disease sites. There was no difference in the osPFS of extra-thoracic sites of disease in patients with high PD-L1 expression. There was significant discordance between visceral disease response and bone disease response to ICI (p=0.047). The presence of BoMs was an independent poor prognostic factor for OS (HR 1.26, 95%CI: 1.05-1.53, p=0.01). Metastatic bone, liver, and intra-abdominal lesions demonstrated inferior clinical responses to ICI relative to other sites of disease. Additionally, the presence of bone and liver metastases were independent poor prognostic factors for overall survival. This real-world data suggests that BoMs respond poorly to ICI and may require treatment adjuncts for disease control.

Thalamic Nucleus Reuniens Glutamatergic Neurons Mediate Colorectal Visceral Pain in Mice via 5-HT2B Receptors

Irritable bowel syndrome (IBS) is a common functional bowel disorder characterized by abdominal pain and visceral hypersensitivity. Reducing visceral hypersensitivity is the key to effectively relieving abdominal pain in IBS. Increasing evidence has confirmed that the thalamic nucleus reuniens (Re) and 5-hydroxytryptamine (5-HT) neurotransmitter system play an important role in the development of colorectal visceral pain, whereas the exact mechanisms remain largely unclear. In this study, we found that high expression of the 5-HT2B receptors in the Re glutamatergic neurons promoted colorectal visceral pain. Specifically, we found that neonatal maternal deprivation (NMD) mice exhibited visceral hyperalgesia and enhanced spontaneous synaptic transmission in the Re brain region. Colorectal distension (CRD) stimulation induced a large amount of c-Fos expression in the Re brain region of NMD mice, predominantly in glutamatergic neurons. Furthermore, optogenetic manipulation of glutamatergic neuronal activity in the Re altered colorectal visceral pain responses in CON and NMD mice. In addition, we demonstrated that 5-HT2B receptor expression on the Re glutamatergic neurons was upregulated and ultimately promoted colorectal visceral pain in NMD mice. These findings suggest a critical role of the 5HT2B receptors on the Re glutamatergic neurons in the regulation of colorectal visceral pain.

Morphological heterogeneity of neurons in the human central amygdaloid nucleus.

The central amygdaloid nucleus (CeA) has an ancient phylogenetic development and functions relevant for animal survival. Local cells receive intrinsic amygdaloidal information that codes emotional stimuli of fear, integrate them, and send cortical and subcortical output projections that prompt rapid visceral and social behavior responses. We aimed to describe the morphology of the neurons that compose the human CeA (N = 8 adult men). Cells within CeA coronal borders were identified using the thionine staining and were further analyzed using the "single-section" Golgi method followed by open-source software procedures for two-dimensional and three-dimensional image reconstructions. Our results evidenced varied neuronal cell body features, number and thickness of primary shafts, dendritic branching patterns, and density and shape of dendritic spines. Based on these criteria, we propose the existence of 12 morphologically different spiny neurons in the human CeA and discuss the variability in the dendritic architecture within cellular types, including likely interneurons. Some dendritic shafts were long and straight, displayed few collaterals, and had planar radiation within the coronal neuropil volume. Most of the sampled neurons showed a few to moderate density of small stubby/wide spines. Long spines(thin and mushroom) were observed occasionally. These novel data address the synaptic processing and plasticity in the human CeA. Our morphological description can be combined with further transcriptomic, immunohistochemical, and electrophysiological/connectional approaches.It serves also to investigate how neurons are altered in neurological and psychiatric disorders with hindered emotional perception, in anxiety, following atrophy in schizophrenia, and along different stages of Alzheimer's disease.

Qizhi Weitong Granules Alleviate Functional Dyspepsia-like Gastric Hypersensitivity in Rats by Inhibiting Inflammatory Cytokines and Strengthening Duodenal Mucosal Barrier

Background: Qizhi Weitong (QZWT) granules are a compound preparation of Chinese medicine, which are approved by theChina Food and Drug Administration for the treatment of functional dyspepsia (FD) and have been used in clinical practice fordecades. The objective of this study is to evaluate whether QZWT granules can alleviate gastric hypersensitivity by improvinglow-grade inflammation and the mucosal barrier in the duodenum in a functional dyspepsia-like (FD-like) model in rats.Methods: FD-like gastric hypersensitivity in adulthood was induced by iodoacetamide (IA) in neonatal rats. Thirty-twoSprague-Dawley (SD) rats were randomly classified into the control, model, QZWT, and Mosapride groups. Further, 0.2 mLof 0.1% iodoacetamide and 2.0% sucrose administrated by oral gavage was used to establish the FD-like rats model (oncea day, for six consecutive days). Electromyography (EMG) was used to evaluate the visceral motor response to gastricdilation. After treatment, the fluorescein isothiocyanate-glucan (FITC-glucan) test was performed to evaluate duodenalpermeability; enzyme-linked immunosorbent assay (ELISA) and quantitative polymerase chain reaction (qPCR) were used toevaluate the pro-inflammatory cytokines for duodenal epithelial cells and the tight junction (TJ) proteins of the duodenalmucosa. Results: The EMG score was elevated in the model group compared to the control group, but it decreased afterQZWT intervention; no changes were observed between the Mosapride and model groups. Compared with the controlgroup, rats in the gastric hypersensitivity model group showed a higher expression of interleukin-6 (IL-6) and tumor necrosisfactor-α (TNF-α) and lower expression of zona occludens protein 1 (ZO-1), occludin, and desmosome (DSG2) of junctionproteins (P < 0.05); while in the QZWT group, the expression of IL-6 and TNF-α was reduced (P < 0.01), and the expressionof ZO-1, occludin, and DSG2 was increased compared to the model group (P < 0.05). Conclusion: QZWT granulesalleviate visceral hypersensitivity in an FD-like model in rats by inhibiting inflammatory cytokines IL-6 and TNF-α andregulating junction proteins.

Regulation of the MCHergic Neural Circuit to Dorsal Raphe Nucleus on Emotion-Related Behaviors and Intestinal Dysfunction in Mice Model of Irritable Bowel Syndrome with Diarrhea

Introduction: Irritable bowel syndrome with diarrhea (IBS-D) is frequently accompanied by depression and anxiety, resulting in a reduced quality of life and increased medical expenditures. Although psychological factors are known to play an important role in the genesis and development of IBS-D, an understanding of the central neural control of intestinal dysfunction remains elusive. Melanin-concentrating hormone (MCH) is a gut-brain peptide involved in regulating feeding, sleep-wake rhythms, and emotional states. Methods: This study investigated the regulation of the MCHergic neural circuit from the lateral hypothalamic area (LHA) to the dorsal raphe nucleus (DRN) on anxiety- and depression-like behaviors, intestinal motility, and visceral hypersensitivity in a mice model of IBS-D. The models of IBS-D were prepared by inducing chronic unpredictable mild stress. Results: Chemogenetic activation of the MCH neurons in the LHA could excite serotonin (5-HT) neurons in the DRN and induce anxiety- and depression-like behaviors and IBS-D-like symptoms, which could be recovered by microinjection of the MCH receptor antagonist SNAP94847 into the DRN. The mice model of IBS-D showed a reduction of 5-HT and brain-derived neurotrophic factor (BDNF) expression in the DRN, while an elevation of 5-HT and BDNF was observed in the colon through immunofluorescent staining, ELISA, and Western blot analysis. SNAP94847 treatment in the DRN alleviated anxiety- and depression-like behaviors, improved intestinal motility, and alleviated visceral hypersensitivity responses by normalizing the 5-HT and BDNF expression in the DRN and colon. Conclusion: This study suggests that the activation of MCH neurons in the LHA may induce IBS-D symptoms via the DRN and that the MCH receptor antagonist could potentially have therapeutic effects.

Heart rate and insula activity increase in response to music in individuals with high interoceptive sensitivity.

Interoception plays an important role in emotion processing. However, the neurobiological substrates of the relationship between visceral responses and emotional experiences remain unclear. In the present study, we measured interoceptive sensitivity using the heartbeat discrimination task and investigated the effects of individual differences in interoceptive sensitivity on changes in pulse rate and insula activity in response to subjective emotional intensity. We found a positive correlation between heart rate and valence level when listening to music only in the high interoceptive sensitivity group. The valence level was also positively correlated with music-elicited anterior insula activity. Furthermore, a region of interest analysis of insula subregions revealed significant activity in the left dorsal dysgranular insula for individuals with high interoceptive sensitivity relative to individuals with low interoceptive sensitivity while listening to the high-valence music pieces. Our results suggest that individuals with high interoceptive sensitivity use their physiological responses to assess their emotional level when listening to music. In addition, insula activity may reflect the use of interoceptive signals to estimate emotions.

Converging neural-centric and mechano-regulation in organoid modeling for system biology and medicine

<p>The understanding of complex biological systems and the development of effective precision medicine strategies necessitate controllable and tractable experimental models. The human body is composed of systemic and systematic interactions at multiple levels such as occurs between cells, tissues, and organs. Hence, how to recapitulate the system complexity of the human body has become an inevitable problem. This review emphasizes the need to understand complex interactions between organs by exploring the potential use of organoids and their derivatives. We focus on the nervous system and its pivotal roles in the regulation of peripheral organs, and meanwhile, highlight the importance of often overlooked mechanobiological factors. The nervous system controls many neuromodulation processes and is capable of transmitting biological information through electrophysiology. In addition, mechano-regulation operates at the cellular and microenvironment levels, functioning through system-level regulation. It can influence neural tissue or collaborate with nerves to direct skin and visceral tissue responses and their immunity. To achieve <i>in situ</i> probing and manipulation of such processes, we recommend the use of organoid assembloids that directly fusion individual organoids to create interactive structures of neural-centric complexes and mechano-regulation conditions, or organoids-on-a-chip that relies on microfluidic chips as tailorable bioreactors to form multi-organ associations and simulate and incorporate mechanobiological and neurological regulations. Based on mechano-regulatory influence at the cellular and microenvironment levels, we may develop more systematic, biomimetic, and robust in-vitro models. These models not only approach genuine physiology and pathology in humans without sacrificing real-time observation capabilities but present minimal ethical concerns and offer substantial potential for industrial scalability.</p>

Intuition as a “trained thing”: sensing, thinking, and speculating in computational cultures

What happens when intuition becomes algorithmic? This article explores how approaching intuition as recursively trained sheds light on what is at stake affectively, politically, and ethically in the entanglements of sensorial, cognitive, computational and corporate processes and (infra)structures that characterise algorithmic life. Bringing affect theory and speculative philosophies to bear on computational histories and cultures, I tease out the continuing implications of post-war efforts to make intuition a measurable and indexable mode of anticipatory knowledge. If digital computing pioneers tended to elide the more ambivalent implications of quantifying intuition, this article asks what computational myths are at play in current accounts of machine learning-enabled sensing, thinking, and speculating and what complexities or chaos are disavowed. I argue that an understanding of more-than-human intuition which grapples meaningfully with the indeterminacy central to digitally mediated social life must recognise that visceral response is recursively trained in multiple ways with diverse, and often contradictory, effects.

Transcriptome and digital gene expression analysis reveal immune responses of mantle and visceral mass pearl culturing in Hyriopsis cumingii

Biomineralization is a widespread phenomenon in marine mollusks and is responsible for the production of shells and pearls. However, the regulatory mechanisms governing the adaptive immune responses in the mollusk mantle and visceral mass during mineralization remain unclear. In this work, we examined the mantle and visceral mass immune responses of Hyriopsis cumingii during pearl culture using high-throughput sequencing techniques. A mantle transcriptome database was established using transcriptome sequencing technology and reference to the major databases. Digital gene expression profiling was used to identify the differentially expressed genes of mantle and visceral mass at different insertion periods. Moreover, quantitative real-time PCR was used to verify the expression of five immune-related genes. Transcriptome sequencing results showed 257,457 unigenes were identified. Digital gene expression profiles showed 1389, 3572, 1888, and 2613 differentially expressed genes (DEGs) in the mantle and visceral mass at 5, 20, 50, and 90 d after insertion, respectively, with the highest number at 20 d and the lowest at 5 d after insertion (q < 0.05). A cluster analysis of the DEGs showed similar clustering and expression features in the mantle to the control group, and at 5, 50 and 90 d, after mantle insertion. The DEGs in the visceral mass showed similar clustering and expression features to the control group and at 5, 20 and 50 d after insertion. We also screened 22 immune-related DEGs in the mantle and visceral mass during the same pearl culture period, including serine/threonine-protein kinase NLK, C-type lectin, and galectin. The greatest number of DEGs was found 90 d after insertion. Compared with the mantle, more immune-related DEGs were down-regulated than up-regulated in the visceral mass during pearl culture, indicating that the immune regulatory mechanisms in the visceral mass and the mantle differ during pearl culture, and that the visceral mass is liable to higher infection and mortality rates. Quantitative real-time PCR results showed that the expression of five immune-related genes was consistent with DGE results. Our findings will further knowledge of the immune systems that are present in the mantle and visceral mass during pearl culture.

Higher Intra-Abdominal Visceral Adipose Tissue Mass Is Associated With Lower Rates of Clinical and Endoscopic Remission in Patients With Inflammatory Bowel Diseases Initiating Biologic Therapy: Results of the Constellation Study

We sought to assess the association between intra-abdominal visceral adipose tissue (IA-VAT) and response to 3 different biologic drugs in patients with inflammatory bowel disease (IBD) and to investigate its effects on inflammatory cytokine expression, pharmacokinetics, and intestinal microbiota. We prospectively enrolled subjects with active IBD initiating infliximab, vedolizumab, or ustekinumab and a healthy control group. Baseline body composition (including IA-VAT as percent of total body mass [IA-VAT%]) was measured using GE iDXA scan. Primary outcome was corticosteroid- free deep remission at weeks 14-16, defined as Harvey Bradshaw Index <5 for Crohn's disease and partial Mayo score <2 for ulcerative colitis, with a normal C-reactive protein and fecal calprotectin. Secondary outcomes were corticosteroid-free deep remission and endoscopic remission (Endoscopic Mayo Score ≤1 in ulcerative colitis or Simple Endoscopic Score for Crohn's disease ≤2) at weeks 30-46. A total of 141 patients with IBD and 51 healthy controls were included. No differences in body composition parameters were seen between the IBD and healthy control cohorts. Patients with higher IA-VAT% were less likely to achieve corticosteroid-free deep remission (P < .001) or endoscopic remission (P= .02) vs those with lower IA-VAT%. Furthermore, nonresponders with high IA-VAT% had significantly higher serum interleukin-6 and tumor necrosis factor at baseline compared with responders and patients with low IA-VAT%. Drug pharmacokinetic properties and microbiota diversity were similar when comparing high and low IA-VAT% groups. Higher IA-VAT% was independently associated with worse outcomes. This association could be driven at least partially by discrete differences in inflammatory cytokine expression.

Why look at dead animals?

AbstractLion Attacking a Dromedary was a sensational object for its first viewers at the Paris Universal Exposition in 1867. As an entity or thing, it provoked a powerfully visceral response and, as the current essay explores, it remains a difficult object to view or display today. As we now know, the Verreaux brothers embedded human remains in the figure of the rider that had formerly been assumed to be just a clothed mannequin. (I have elected to exclude any image of this controversial diorama containing human and other animal remains because it is not necessary for this theoretical inquiry). This essay suggests that theoretical tools derived from Material Ecocriticism and Monster Theory that may help us to think about, or alongside, the affective power of this disturbing taxidermy assemblage, ever aware that this piece draws its power from the theatrical, colonial violence of extraction and extinction.

An anesthesia protocol for robust and repeatable measurement of behavioral visceromotor responses to colorectal distension in mice.

Visceral motor responses (VMR) to graded colorectal distension (CRD) have been extensively implemented to assess the level of visceral pain in awake rodents, which are inevitably confounded by movement artifacts and cannot be conveniently implemented to assess invasive neuromodulation protocols for treating visceral pain. In this report, we present an optimized protocol with prolonged urethane infusion that enables robust and repeatable recordings of VMR to CRD in mice under deep anesthesia, providing a two-hour window to objectively assess the efficacy of visceral pain management strategies. During all surgical procedures, C57BL/6 mice of both sexes (8-12 weeks, 25-35 g) were anesthetized with 2% isoflurane inhalation. An abdominal incision was made to allow Teflon-coated stainless steel wire electrodes to be sutured to the oblique abdominal musculature. A thin polyethylene catheter (Φ 0.2 mm) was placed intraperitoneally and externalized from the abdominal incision for delivering the prolonged urethane infusion. A cylindric plastic-film balloon (Φ 8 mm x 15 mm when distended) was inserted intra-anally, and its depth into the colorectum was precisely controlled by measuring the distance between the end of the balloon and the anus. Subsequently, the mouse was switched from isoflurane anesthesia to the new urethane anesthesia protocol, which consisted of a bout of infusion (0.6 g urethane per kg weight, g/kg) administered intraperitoneally via the catheter and continuous low-dose infusion throughout the experiment at 0.15-0.23 g per kg weight per hour (g/kg/h). Using this new anesthesia protocol, we systematically investigated the significant impact of balloon depth into the colorectum on evoked VMR, which showed a progressive reduction with increased balloon insertion depth from the rectal region into the distal colonic region. Intracolonic TNBS treatment induced enhanced VMR to CRD of the colonic region (>10 mm from the anus) only in male mice, whereas colonic VMR was not significantly altered by TNBS in female mice. Conducting VMR to CRD in anesthetized mice using the current protocol will enable future objective assessments of various invasive neuromodulatory strategies for alleviating visceral pain.

Evaluation of two laboratory model methods for diarrheal irritable bowel syndrome

BackgroundDiarrheal irritable bowel syndrome (IBS-D) is a common chronic functional gastrointestinal disorder, and the underlying pathogenic mechanism is still unclear. Animal models that mimic the pathological state of IBS-D patients were constructed to provide a reference for later drug research and model development.MethodsThe IBS-D model was induced using restraint stress and chemical stimulation (rhubarb), and rats were divided into normal control group (NC), chemically stimulated group (CS), and restraint stress group (RS). Visceral motility responses to Colorectal Balloon Dilation (CRD) were measured by Abdominal Withdrawal Reflex (AWR); evaluation of faecal properties and water content; determination of colonic tissue tight junction (TJ) mRNA expression by RT-PCR; measurement of inflammatory cytokines by ELISA; and intestinal flora and short chain fatty acids.ResultsCompared to NC group, CS and RS group rats showed increased intestinal sensitivity and Bristol stool score, significant diarrheal symptoms and weight loss. Mucin 2, ZO-1, OCLN, CLDN4 mRNA expression was reduced and the intestinal mucosal barrier function was diminished. In addition, the levels of inflammatory factors IL-1β, IL-6, IL-8, IL-10 and TNF-α increased, the abundance and diversity of intestinal flora decreased, the content of beneficial bacteria such as Bifidobacteria decreased, and SCFAs such as acetic acid, propionic acid and butyric acid decreased to different degrees. Although, no significant difference was observed for any molecular and inflammatory marker, but compared to CS group, RS group had less water in the stool, higher visceral sensitivity, and higher relative abundance of beneficial intestinal bacteria such as Actinobacteria.ConclusionIn conclusion, restraint stress combined with chemical stimulation can mimic the pathological state of diarrhoea symptoms, visceral hypersensitivity, reduced intestinal mucosal barrier permeability, immune regulatory dysfunction and dysbiosis in IBS-D patients. However, herbs with antibacterial effects such as rhubarb and senna, for example, are not suitable as the first choice for chemical stimulation, as they may lead to a decrease in harmful bacteria and an increase in beneficial bacteria in the intestinal fraction and do not perfectly mimic the imbalanced state of intestinal flora in IBS-D patients, while restraint stress may be a key factor in modelling.

Moving-With Anastasis Corporal, a path to implicated witnessing

ABSTRACT This article thinks through a practice-as-research exploration of facilitating active listening to the testimonies of female victims/survivors in Colombia, bridging a theoretical gap between applied theatre and performance studies by extending understanding of theatre’s impact in contexts of transitional justice beyond visibility to an affective register. Rebuilding relationalities torn by war requires collective healing to overcome widespread trauma, disconnection and apathy. By moving-with recorded testimonial performances, sharing visceral responses via Zoom, witnesses feel more implicated in histories of violence. Fomenting shared response-ability in this way – through listening from the body – offers a form of resistance to the normalisation of violence.

MicroRNA-155-5p inhibition alleviates irritable bowel syndrome by increasing claudin-1 and ZO-1 expression.

Irritable bowel syndrome (IBS) is a common gastrointestinal disease. Emerging studies have demonstrated that microRNAs (miRNAs) are commonly dysregulated in patients with IBS, and aberrant miRNAs are implicated in IBS occurrence. Although miR-155-5p participates in inflammatory bowel disease (IBD) and intestinal barrier dysfunction, the role of miR-155-5p in IBS is unclear. In the present study, colon samples were obtained from IBS patients and IBS mice induced by trinitrobenzenesulfonic acid (TNBS), and the levels of miR-155-5p, claudin-1 (CLDN1), and zonula occludens-1 (ZO-1) were assessed using quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemical analysis. The regulatory role of miR-155-5p in CLDN1 and ZO-1 expression was validated using dual luciferase reporter assay. We found that miR-155-5p levels were upregulated in colon samples of IBS patients and mice compared with healthy subjects and normal mice, respectively. Meanwhile, the levels of CLDN1 and ZO-1 were decreased in colon samples of IBS patients and mice. Importantly, forced expression of miR-155-5p inhibited CLDN1 and ZO-1 expression. In IBS mice, intraperitoneal injection with miR-155-5p inhibitor increased CLDN1 and ZO-1 expression in intestinal mucosal epithelium, enhanced visceral response thresholds, and decreased myeloperoxidase (MPO) activity. In summary, these results suggested that miR-155-5p participated in the pathogenesis of IBS, at least in part by inhibiting CLDN1 and ZO-1 expression, indicating that miR-155-5p may be a potential therapeutic target for IBS.

A bibliometric analysis of 100 top-cited journal articles related to acupuncture regulation of the autonomic nervous system.

Research on the effects of acupuncture on autonomic function has been conducted for several decades, and a few notable studies have emerged in recent years. This study used bibliometric analysis to assess 100 top-cited articles to characterize the current status and research trends over the last three decades. The 100 top-cited publications were identified from the Web of Science Core Collection database. The bibliometrix package in R was used for quantitative and qualitative analyses of the publication patterns and the country/region, institution, and author contributions. VOSviewer was used to construct networks based on co-citation analysis of the journals and the keyword co-occurrence. The 100 top-cited articles were identified with a total of 8,123 citations (range: 37-345). The majority of the articles came from the USA (n = 42), followed by Japan (n = 14) and mainland China (n = 13). Articles from the USA exhibited the largest number of citations (3,582 citations), followed by articles from Japan (1,189 citations), then articles from mainland China (755 citations). Neurosciences/Neurology was the most studied research area (n = 41). The Autonomic Neuroscience: Basic and Clinical published the largest number of papers (n = 14), while Brain Research received the largest number of citations (205 citations). Longhurst JC was the most productive author (10 publications), and Sato A was first among the cited authors (87 citations). The most frequently cited articles that focused on gastrointestinal, cardiovascular, or gynecologic responses to acupuncture regulation of the autonomic nervous system first appeared in the 1990s, peaked in the 2000s, then decreased after 2010. Publication of articles focused on the anti-inflammatory effects of acupuncture associated with autonomic function demonstrated an increasing trend over the last three decades. From the initial studies focusing on the autonomic mechanism of visceral responses to acupuncture, researchers concentrated on exploring the autonomic mechanism of acupuncture in the control of systemic inflammation. Non-invasive electrical methods that activate somato-autonomic reflexes are current translational directions in clinical practice. Additional investigation of the underlying neuroanatomical basis of somato-autonomic reflexes also is needed.

The Emergence of a Theatrical Science of Man in France, 1660–1740

The Emergence of a Theatrical Science of Man in France, 1660–1740