Viscera Of Sheep Research Articles

Increased prevalence of canine echinococcosis a decade after the discontinuation of a governmental deworming program in Tierra del Fuego, Southern Chile.

Hydatid disease is a neglected zoonotic parasitic disease caused by cysts of the tapeworm Echinococcus granulosus. Canids, especially domestic dogs, are definitive hosts of the parasite and are the most pragmatic targets for control programs. A governmental dog deworming campaign was established in 1979 to control hydatidosis in southern Chile, which succeeded in reducing the prevalence of canine echinococcosis in Tierra del Fuego province from 68.4% (in 1978) to 1.2% (in 2002). In 2004, however, the program was dismantled to reduce costs, and since then, no follow-up echinococcosis monitoring has been conducted. We surveyed 356 domestic dogs and interviewed owners or workers at 45 ranches in Chilean Tierra del Fuego during the summer of 2015-2016. Faecal flotation was employed to detect Taeniidae eggs, and PCR was used to test faecal samples for Echinococcus granulosus. Taeniidae eggs and Echinococcus sp. DNA were detected in the faeces of 45.4% (147/324) and 6.9% (23/331) of dogs, respectively. Infrequent dog deworming and the presence of culpeo foxes (Lycalopex culpaeus) were significant predictors of the prevalence of Echinococcus sp. DNA and Taeniidae eggs. Furthermore, the presence of introduced chilla foxes (Lycalopex griseus), the municipality, and several operational characteristics of ranches (number of sheep, frequency of sheep slaughter, number of dogs, frequency of removal of dog faeces, feeding of dogs with sheep viscera) were also predictive of the prevalence of Taeniidae eggs. Our findings reveal an ongoing risk of echinococcosis with pathogen maintenance in ranch dogs in Chilean Tierra del Fuego, and in the absence of adequate control programmes, there is a tangible risk of re-emergence of hydatid disease as a public health concern.

Survey for Cadmium Concentration in Liver, Kidney and Muscle of Slaughtered Sheep in Butcher Shops of Fallujah City, Iraq

Cadmium (Cd) is regarded as one of major the presence of hazardous substances in the environment, with a broad variety toxicity to organs and an extended half-life of removal. Cd exposure for farmed ruminants can come from industrial processing and intensive agricultural operations, which can pollute feed and water. It was noted that most of the wastes produced by nearby factories in Al Fallujah city are either strewn around the highways or use as landfills while the sewage is used for irrigation, while sheep graze on the edges of runways and other sites that might have been contaminated with hazardous materials. EF (enrichment factor) According to the findings, the concentration of Cd in the soils of Al Fallujah city was exceptionally high, and according to sediment content criteria, the soils in Fallujah city were contaminated with high concentrations of Cd, revealing the influence of human and industrial activity on Cd accumulation in the soil. Cadmium levels in liver, kidney and muscle from 216 samples for 72 slaughtered sheep in slaughterhouse at Al Fallujah city, Iraq during the period of October to December 2020 were analyzed. The values of Cadmium detected in sheep liver, kidney and muscle samples were 0.138± 0.020, 0.432 ± 0.090, and 0.037± 0.003 mg/kg, respectively which refer to high concentration of Cd in sheep viscera also This study demonstrates the need of assessing and monitoring cadmium and other hazardous metal levels in meals in order to create population-safe intake values.

Effect of Genetic Crossing and Nutritional Management on the Mineral Composition of Carcass, Blood, Leather, and Viscera of Sheep.

The effect of genetic crossing and nutritional management on weight gain and the concentration of minerals and trace elements in the carcass, blood, leather, and viscera of sheep were evaluated. Several statistical strategies were used to evaluate the different elemental composition characteristics of pure breed animals, i.e., White Dorper (ODO), Ile de France (OIF), Texel (OTX), and Santa Inês (OSI), as well as their crossbreeds 1/2 White Dorper and 1/2 Santa Inês (ODS), 1/2 Ile de France, and 1/2 Santa Inês (OIS), 1/2 Texel × 1/2 Santa Inês (OTS). Three different diets were evaluated AL (ad libitum), R75, and R63 (75 and 63g of dry matter/kg of the animal metabolic weight, respectively). Levels of Ca, Cu, Fe, K, Mg, Mn, P, S, and Zn were determined by inductively coupled plasma optical emission spectrometry (ICP OES). The concentration of inorganic elements in the different body components was not affected by the diet (P> 0.05), and OTX and OTS were the breeds with the highest weight gain. Random forest importance models demonstrated that Zn in the carcass, K, Ca, and Zn in blood, and K in leather are most associated with separating the different evaluated sheep's breeds. Crossbreeding the native Santa Inês breed with sheep of exotic breeds produces animals well adapted to confinement.

225 Crude protein-content of fat-free muscle and viscera in sheep

Abstract The nutritional effects of variation in feed supply and subsequent compensatory gain can play a significant role in cattle and beef production, due to their effects on carcass quality and feed costs. A system that predicts changes in fat and protein content of muscle and viscera in animals of different life stages and nutritional histories could therefore assist management to optimize performance and reduce costs of feed. A method has been developed to simultaneously estimate body composition and nutrient requirements of ruminants. This method estimates body composition from the difference in energy balance derived from ME intake, and heat production from ME intake and protein content of muscle and viscera, but requires information on protein content of fat free mass in viscera and non-viscera “muscle” tissue. Data from the literature was combined with unpublished data from a study conducted in growing lambs. This experiment tested the effects of ad libitum intake of diets of varying energy density and added RUP on performance and carcass composition of lambs that had been previously restricted or unrestricted prior to a 12–13 week finishing phase, and both carcass and viscera components were chemically analyzed on an individual basis. On a fat-free basis, muscle crude protein averaged 20.8%; this value is in agreement with literature values, which lie between the range of 20–24% and which do not appear to vary substantially with age in postweaning sheep. In the same sheep, crude protein content of viscera averaged 15.6% on a fat-free basis; while data on visceral composition in the literature is limited, these values are within the range of available data. This data is use to parameterize functions describing growth of viscera and changes in heat production over time. This contributes to the method we have developed to estimate nutritional effects on body composition.

Lipid Production from Amino Acid Wastes by the Oleaginous Yeast Rhodosporidium toruloides

Microbial lipids have been considered as promising resources for the production of renewable biofuels and oleochemicals. Various feedstocks, including sugars, crude glycerol, and volatile fatty acids, have been used as substrates for microbial lipid production, yet amino acid (AA) wastes remain to be evaluated. Here, we describe the potential to use AA wastes for lipid production with a two-stage culture mode by an oleaginous yeast strain Rhodosporidium toruloides CGMCC 2.1389. Each of the 20 proteinogenic AAs was evaluated individually as sole carbon source, with 8 showing capability to facilitate cellular lipid contents of more than 20%. It was found that L-proline was the most favored AA, with which cells accumulated lipids to a cellular lipid content of 37.3%. When blends with AA profiles corresponding to those of meat industry by-products and sheep viscera were used, the cellular lipid contents reached 27.0% and 28.7%, respectively. The fatty acid compositional analysis of these lipid products revealed similar profiles to those of vegetable oils. These results, thus, demonstrate a potential route to convert AA wastes into lipids, which is of great importance for waste management and biofuel production.

Evaluation of equations to estimate fat content in soft tissues of carcasses and viscera in sheep based on carbon and nitrogen content

The objective of the present study was to evaluate equations for estimating fat content (%) of soft tissues of carcass (muscle and adipose tissues, n = 28) and viscera (n = 28) samples of adult ewes based on nitrogen (N, %), carbon (C, %) and dry matter (DM, %) composition. The following equations reported by Brouwer (1965) and Kuhla et al. (2004) were tested: ether extract (EE, %) = 1.3038 × C (%) − 4.237 × N (%) (eq. 1), EE (%) = 1.2948 × C (%) − 4.407 × N (%) (eq. 2) and EE (%) = −31.23 + 1.9677 × DM (%) (eq. 3). A fourth equation for estimating EE was also tested: EE (%) = 1.3039 (± 0.017) × C (%) – 3.9949 (± 0.218) × N (%) (eq. 4). A final equation including DM content was also developed and tested in the present study: EE (%) = 1.3820 (± 0.025) × DM (%) (eq. 5). The correlation coefficients (r) between EE and C and between EE and N were 0.90 and −0.97, respectively (P < 0.001). All of the equations presented high precision (r2> 0.91), except equations (3) and (5), and high accuracy according to the bias correction factor (Cb > 0.95), confirming their good reproducibility and concordance with the observed data (CCC > 0.94). The modelling efficiency factor (MEF) indicated moderate to high efficiency of prediction (from 0.63 to 0.91) for all equations except equation (3). The coefficient of model determination (CD) ranged from 0.59 to 1.25, indicating high variability in the predicted data. The null hypothesis (intercept = 0 and slope = 1) was only accepted for equations (1), (4) and (5). Equations (1) and (4) had high precision (r2 = 0.92) and accuracy (Cb = 0.99), with a reproducibility index of 0.95. Hence, we conclude that equations (1) and (4) could be used to predict the percentage of EE in sheep soft tissues of carcasses and viscera based on the C and N contents (%). These equations could be applied in the future to determine the composition of the soft tissues of carcass and viscera of animals to estimate their energy requirements under different production levels.

Determining the sensitivity of abattoir surveillance for ovine Johne's disease

The objective of this study was to determine the sensitivity of abattoir surveillance of intestinal tract lesions for detecting ovine Johne's disease (OJD) under normal meatwork conditions. The design of this study was a diagnostic test validation. The three OJD inspectors were the diagnostic test and follow-up histopathological examination was used for test validation. Approximately 1200 sheep were procured from known high prevalence OJD infected farms. The sheep viscera were tagged (numbered) and then examined as they were processed on the abattoir line by three experienced meat inspectors. Their observations were independently recorded on a cassette tape. Specified sections of viscera were prepared and subjected to histopathological examination and these results were compared with the inspector diagnoses. The sensitivity of abattoir inspection for OJD varied between inspectors from 53 percent to 87 percent. The specificity varied from 97 to 100 percent. It appeared that the level of sensitivity for detecting disease was higher in lines of sheep where the disease was more prevalent. It also appeared that formal training was an important aspect in ensuring a high level Abattoir surveillance is a very economical and rapid method of assessing the OJD status of sheep. On the basis of these results it is reasonable to suggest that abattoir surveillance has a sensitivity of approximately 70 percent. This technique is useful as an ancillary to other testing regimes for negative assurance programs where a sheep identification system is used.

Digestion and nutrient net fluxes across the rumen, and the mesenteric- and portal-drained viscera in sheep fed with fresh forage twice daily: net balance and dynamic aspects.

Digestion and portal net flux of nutrients were studied in sheep fed twice daily with fresh orchard-grass. Digestive flows were measured in six fistulated sheep using the double-marker technique. Three sheep were fitted with catheters and blood-flow probes, allowing nutrient net flux measurements across the portal-drained viscera (PDV), the mesenteric-drained viscera (MDV) and the rumen. Total tract apparent digestion of N was similar to portal net appearance of N, calculated as the sum of free amino acids (FAA), peptide amino acids (PAA), NH3, and urea net fluxes. PAA accounted for 25 % of non-protein amino acid net release across the PDV. With the exception of glycine and glutamate, the small intestine was the main contributor to this PAA net release. The essential amino acid (EAA) apparent disappearance between the duodenum and the ileum was lower than the net appearance of EAA (FAA + PAA) across the MDV. The value of PDV:MDV flux of free EAA was, on average, 78 %. The rumen accounted for 30 % of the net uptake of EAA by the PDV tissues not drained by the mesenteric vein. Rumen net release of acetate, propionate, butyrate, 3-hydroxybutyrate, and lactate accounted for 70, 55, 46, 77 and 52 %, respectively, of their portal net releases. Conversely, the small intestine was a net consumer of arterial acetate and 3-hydroxybutyrate. Dynamic study of nutrient net fluxes across the PDV showed that throughout a feeding cycle, the liver faced a constant flux of amino acids (AA), whereas volatile fatty acid and NH3 net fluxes varied in response to the meal. The present study specified, in forage-fed sheep, the partitioning of nutrient net fluxes across the PDV and the role of peptides in portal net release of AA.

Absorption of amino acids from the intestine and their net flux across the mesenteric- and portal-drained viscera of lambs.

Experiments were conducted to compare the rates of apparent absorption (disappearance) of individual essential amino acids (EAA) from the small intestine with their net fluxes across the mesenteric- (MDV) and portal- (PDV) drained viscera in sheep given a pelleted alfalfa diet at two levels of intake. Disappearances of individual EAA across the region of the small intestine drained by the mesenteric arcade (jejunum to ileum) were similar to those across the whole of the small intestine (duodenum to ileum). The net MDV flux of each EAA was similar to its rate of disappearance, but, with the exception of threonine on the low intake level, the net PDV flux was lower (P < .05). Increasing the intake of alfalfa from 800 to 1,200 g/d increased the rate of disappearance of individual EAA between the duodenum and ileum by .56 (range .43 to .65) and between the jejunum and ileum by .51 (range .45 to .60). The MDV and PDV blood flows increased by .35 and .39, respectively, and, with the exception of valine, net fluxes of individual EAA increased by .39 (range .20 to .50) across the MDV and by .44 (range .21 to .71) across the PDV. When net fluxes across the MDV and PDV were measured simultaneously, the ratio of PDV: MDV flux for each EAA was less than (P < .05) unity (mean .61, range .55 to .69), even though all MDV blood enters the PDV, contributing approximately .45 of the total portal flow. This observation suggests that, in regions of the tract not drained by the MDV, extraction of arterial blood EAA for tissue and secretory protein synthesis must exceed the release of protein degradation products into the venous drainage. The results are discussed in terms of endogenous protein secretions into the lumen of the tract anterior to the small intestine.

Effect of intravenous glucose infusion on metabolism of portal-drained viscera in sheep fed a cereal/straw-based diet2

This experiment investigated the effect of intrajugular infusion of glucose on whole-body glucose metabolism and the absorption of nutrients by the portal-drained viscera of four Suffolk-cross sheep, average BW 46 +/- 7 kg, fed a cereal/straw-based pelleted diet. Each sheep received by random allocation 0 (control), 1.0, or 2.0 mg of glucose.kg BW-1.min-1 for 8 h infused into the jugular vein. Glucose irreversible loss rate, measured by simultaneous infusion of 6-[3H]-glucose, increased approximately stoichiometrically during glucose infusion (.51, .75, and 1.09 error mean square [EMS] .032 mmol/min for control, 1.0, and 2.0 mg of glucose infused.kg BW-1.min-1, respectively). Utilization of glucose increased in portal tissues (P = .089) as a result of glucose infusion (.17, .18, and .33 EMS .008 mmol/min) and was a constant proportion of glucose irreversible loss (.28). Portal blood flow was not affected by glucose infusion (overall mean 1.13 L/min EMS .034). Net portal absorption of acetate increased during glucose infusion although ruminal VFA concentrations were not affected. In contrast, net free amino acid absorption by portal-drained tissues was reduced during glucose infusion (291, 115, and 4 EMS 33,816 mumol/min, P = .054). These results show that metabolism of nutrients across the gut wall is influenced by glucose availability to gastrointestinal tissues and affects the pattern of nutrients available to the liver and peripheral tissues.

Epidemiologic investigation of echinococcosis in American Indians living in Arizona and New Mexico.

Ten cases of echinococcosis diagnosed in American Indians in Arizona and New Mexico between 1972 and 1975 were investigated as part of a regional epidemiologic study. Patients were visited at home to discover factors associated with local parasite transmission, to detect possible additional cases among family members, and to perform diagnostic tests on dogs. Six patients were Navajo, 2 Zuni, and 2 Santo Domingo Indians. An additional case in a Navajo man was detected by serologic testing of patients' family members; this was the 20th case diagnosed in the region since 1965. Dogs owned by three of the Navajo patients were infected with Echinococcus granulosus. Arecoline-purge testing of 110 dogs in the Zuni pueblo demonstrated echinococcosis in a single stray dog. The findings at slaughter of Navajo-owned sheep indicate that the infection is enzootic in this intermediate host. The epidemiologic findings suggest that humans were infected from dogs which contracted their infections from two sources. The first was sheep raised locally in rural areas of the Navajo Reservation where the infection is enzootic in the dog-sheep cycle; transmission was apparently facilitated by the widespread practice of home butchering. A second source of human infection was dogs which became infected by eating viscera of sheep of off-reservation origin; these sheep were purchased and butchered by individual families in urban areas of the Navajo Reservation and in the Zuni and Santo Domingo pueblos.

Metabolism of volatile fatty acids by liver and portal-drained viscera in sheep.

Metabolism of volatile fatty acids by liver and portal-drained viscera in sheep.

Hydatid Disease in England

Hydatid disease is not a rare disease in this country, although its frequency is much less than in Iceland and in the countries with extensive sheep farming—for example, Australia and the Argentine. While investigating the pathological conditions of food animals, I visited the Birmingham public abattoir daily for several years, and every day saw the viscera of sheep, cattle, or pigs infested with the parasite of this disease. Some herds were extensively infested, others free. In few cases only a solitary cyst was discovered in the animal; in the majority the cysts were multiple, almost completely destroying the normal tissue. The organs so infested were greatly increased in bulk and weight; in some cases cows' livers weighing 200 to 250 lb. were seen, the normal being about 14 lb. (Figs. 1 and 4). Examples of human infestation are seen in hospitals from time to time, and these always attract attention by the variety of the manifestations of the parasitic invasion and by the interesting characteristic features of the hydatid cyst and its contents. No fewer than three hundred articles relative to cases of hydatid disease have been published during the last five years. That we see the disease so frequently in food animals and occasionally in man indicates that conditions are allowed to exist which permit of the completion of the life-cycle of a parasite capable of producing a very dangerous disease in human beings.