Recent trends show a rise in stress and psychological disorders, necessitating innovative strategies for recovery. Our study examines the psychophysiological effects of virtual reality (VR) nature, based on the biophilia hypothesis, within real-life group settings. A randomized, acute cross-over design was employed, involving 57 participants who experienced a 10-min session in either a VR nature or a reference room, separated by a 2-min interval. Participants were measured in groups of 7–10 individuals. The VR setup featured a landscape video projected around the participants, creating a captivating 3D environment, whereas the reference room utilized the same space but without the VR elements, maintaining a basic meeting room atmosphere with white walls and a quiet setting. The primary outcome measured was heart rate variability (HRV), complemented by exploratory outcomes including the heart and respiratory rates, and questionnaires on affective well-being, creativity, and sense of belonging. Findings revealed that VR nature exposure resulted in higher HRV and reduced heart rate, indicative of enhanced parasympathetic activity. Participants reported decreased feelings of anxiety and depression, with an increase in comfort, enthusiasm, creativity, and belonging. These results present the VR nature's physiological and psychological benefits, demonstrating its effectiveness in promoting relaxation and recovery in a real-life group setting. In conclusion, the VR nature room can emerge as a versatile tool for stress alleviation and well-being improvement.