Magnetic field-driven microrobotic swarms have drawn extensive attention, especially in the field of automatic control. Realizing dynamic path planning and motion control of microrobotic swarms for mobile target tracking is one of the important tasks that still remains unsolved. In this paper, we firstly present an enhanced bidirectional rapidly-exploring random tree star (EB-RRT*) algorithm considering the physical size of the swarm to dynamically plan the optimal path for obstacle avoidance. An image-guided motion controller, which consists of a direction controller and a Genetic Algorithm based Linear Quadratic Regulator (GA-LQR) velocity controller, is then proposed to realize mobile target tracking using microrobotic swarms. Targeted bursting algorithm is subsequently developed to meet the requirement of tracking high-speed ( <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">i.e.</i> , 20 <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$\mu m/s$</tex-math> </inline-formula> ) mobile targets. Simulations are performed to validate the proposed methods and obtain the proper ranges of the input parameters for the controllers. Finally, the control effectiveness of mobile target tracking in different conditions and environments is validated by experimental results. <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">Note to Practitioners</i> —The motivation of this work is to develop an effective control scheme for mobile target tracking using microrobotic swarms. Conventional control schemes mainly focus on the control of single microrobots to reach static targets, and thus the desired path is fixed once planned. In addition, the motion of single monolithic microrobots can be modelled precisely. However, in mobile target tracking using microrobotic swarms, dynamic planning algorithms are demanded to frequently update the desired path. Swarms consisting of millions of micro-agents are also difficult to be modelled due to the complex agent-agent interactions. In this work, an effective control scheme consisting of a dynamic path planner, a motion controller and a targeted bursting unit is developed. Real-time dynamic paths will be planned even though the positions of the swarm and the target change rapidly. The precise control of the swarm direction and velocity are achieved, and moreover, using the targeted bursting algorithm, the swarm can be accelerated to approach mobile targets accurately with higher efficiency. Experimental results validates the proposed tracking strategy in different environments with virtual obstacles. The proposed control scheme paves the way for a better understanding of advanced motion control methods for microrobotic swarms.