ObjectiveTo investigate the relationship between patient perception of lower extremity function and a home-based virtual clinician assessment of mobility in lower-limb prosthesis clients. DesignDescriptive observational study using a clinician-administered functional mobility survey and timed-up-and go test to assess lower extremity function under supervision. SettingHIPAA compliant online virtual platform. ParticipantsTwelve lower limb loss clients currently using prostheses, at least 19 years of age; not pregnant, with no stroke, seizure disorder, or cancer. InterventionsNot applicable. Main Outcome Measure(s)Main outcomes were mobility survey scores and mean timed-up-and-go duration. ResultsMost participants reported significant ease of completing basic indoor ambulation and toileting tasks (66 – 75%) and significant difficulty in running or prolonged ambulation activities (83%) requiring use of lower limb prosthesis. Timed-up-and go test was faster (11.0 ± 2.9 seconds) than the reference range for transtibial prosthesis users and negatively associated with self-reported lower extremity functional status (r = -.70, p = .02). ConclusionSelf-reported movement with lower limb prosthesis at home and virtual evaluation of mobility via a virtual platform, is a feasible assessment modality that may reduce frequency of therapy visits, defray some rehabilitation costs, and minimize the travel burden to distant prosthetic clinics.