Vineyards In Spain Research Articles

International Advances in Pesticide Application 2024

Meeting report from Graham Matthews The Association of Applied Biologists latest meeting on advances in application technology, held in Brighton from 23???25 January 2024, was attended by about 80 delegates representing many different countries, with another 30 registered on-line. Mireia Torres Maczassek from Familia Torres vineyards in Spain gave the opening presentation, in which she referred to changing climate and various innovations, regenerative agriculture, adapting vineyards, and, improving the management and purification of water.

Drivers of winegrowers' decision on land use abandonment based on exploratory spatial data analysis and multilevel models

The frequency of producers opting to abandon agricultural land has become increasingly, highlighting the significance of this phenomenon due to its environmental, landscape, and socio-economic impacts. The decisions of producers to abandon or maintain/improve their farms depend on individual and contextual factors. The aims of this research are twofold. Firstly, to evaluate the influence of the neighbours on the winegrowers' decisions, using spatial analysis. Secondly, to clarify the specific importance of each of the individual and contextual drivers in farmers' decisions to improve their farms, to keep them unchanged or to abandon them, using multilevel models. The results obtained for the case study of vineyards in Spain, reveal a strong agglomeration phenomenon in farmers' decisions indicating that producers make land use decisions influenced by what their neighbours do. A multilevel analysis identifies that individual factors are determinant and that the influence of contextual factors is conditioned by the innovation process at farm level. Individual drivers, such as size, innovation, Protected Designations of Origin and irrigation influence vineyard area, with irrigation having the greatest overall influence, and is expected to be decisive in climate change projections. The Protected Designations of Origin are driving forces that dynamize the territory and achieve productive concentrations, encouraging winegrowers to replant, but they are not enough to halt abandonment. The elements that slow down the abandonment of plots are irrigation and the combination of innovation and context variables, mainly the combination of modernised plots in the municipalities with trading options.

Dynamics of Cynodon dactylon and weed community composition in different cover crops in a vineyard

AbstractThe implementation of cover crops in vineyards is an environment‐friendly soil management technique that has several advantages, including weed suppression. The effectiveness of the management depends on the characteristics of both the weed community and the cover crop species. The objectives of this study were to evaluate the dynamics of Cynodon dactylon patches and the rest of the weed community composition under five different cover crops (four grasses and one dicotyledonous species) in a commercial vineyard in Spain: (1) the perennial Festuca arundinacea and the annual species (2) Hordeum vulgare, (3) Vulpia ciliata, (4) a mixture of Bromus species and (5) Medicago rugosa. The annual species were sown in November every year after tillage (from 2015 to 2018) and shredded in June, while F. arundinacea was sown in 2015 and shredded in June and in November every year. Results showed that cover crop species affected C. dactylon soil coverage and frequency across the field. After three seasons, F. arundinacea and H. vulgare were the best cover crops which controlled C. dactylon, resulting in the lowest weed coverage of 4.6% and 3.0% respectively. Although the expansion of the weed was restrained by cover crops, the soil tillage prior to sowing the annual species spreads the rhizomes and stolons to weed free areas of the field. Canonical Correspondence Analysis (CCA) showed that the weed community composition varied depending on the cover crop species, revealing the importance of its management in the weed assemblage.

Mapping the spatial variability of Botrytis bunch rot risk in vineyards using UAV multispectral imagery

The fungus Botrytis cinerea causes severe diseases in many crops. In grapevines, it causes Botrytis bunch rot (BBR), one of the most reported diseases worldwide. It affects all herbaceous organs of the vine, especially the ripe berries, causing significant reductions in yield and wine quality. Botrytis detection models traditionally focus on temporal analysis at a specific spatial location, ignoring the study of the spatial variability of the crop. Unmanned aerial vehicles (UAVs) equipped with multispectral cameras can provide high-resolution images that can be valuable information to develop a tool for aerial pest detection. This paper proposes an algorithm to assess the risk of Botrytis development in a vineyard in Spain, using as input products generated by UAV imagery: DTM (Digital Terrain Model), NDVI (Normalised Difference Vegetation Index), CHM (Canopy Height Model) and LAI (Leaf Area Index). They represent the height and architecture of the canopy, the topography and the plant status. Healthy vines were significantly different from vines affected by Botrytis (p < 0.05) in each of these variables, supporting the consistency of using these inputs for the model. This methodology combines photogrammetric, spatial analysis techniques, and machine learning classification methods with deep vineyard-related agronomic knowledge to produce heatmaps with acceptable accuracy (R² > 0.7) that may support vineyard managers in understanding the spatial variability of the disease, allowing the spatial 2D visualisation of the risk of BBR disease development and, potentially, resulting in higher operational efficiency and reducing phytosanitary treatments, as well as economic costs. Furthermore, the present work takes advantage of imaging technologies that provide information about any location in the field, not only about specific points in the vineyard, suggesting that UAV imagery is appropriate to measure the likelihood of BBR development within the vineyard, highlighting the importance of efficient disease management based on spatial variability.

Carbonated Irrigation Assessment of Grapevine Growth, Nutrient Absorption, and Sugar Accumulation in a Tempranillo (Vitis vinifera L.) Vineyard

Iron chlorosis is common in vineyards grown on calcareous soils, and consists of a chlorophyll deficiency caused by a disorder in iron nutrition. It is manifested by interveinal yellowing of the youngest leaves and decreased growth and production. Most of the vineyards in Spain are planted on calcareous soils, so iron chlorosis is frequent, even in tolerant rootstocks. In the case of Spain, Tempranillo accounts for 20.8% of the total Spanish wine area, with this red variety being the most planted. The study of iron chlorosis and the availability of nutrients is essential for improving the qualitative balance of the wine, as it is one of the most representative in our country. The aim of this work was to evaluate how carbonated irrigation modifies soil pH and increases the absorption of nutrients, as well as to assess the impact of chlorosis on the physiology of the variety and the composition of the grape. In the first instance, a test was carried out on bare soil of calcareous composition, evaluating three different levels of carbonated water, and seeing what response it gave to the pH level. The result showed that the dose of 400 ppm of CO2 provided the optimal pH. Subsequently, in a potted soil, the dose of 400 ppm of CO2 was evaluated on the variety cv. Tempranillo, in three different compositions of calcareous soil. Results showed that carbonated irrigation increased the levels of chlorophyll impacting on primary metabolism (acids and sugar), plant growth, and higher crop yield, improving the optimal grape ripeness. Given that irrigation with 400 ppm CO2 increased the ability to control ferric chlorosis, this strategy could be an easy-to-use alternative to iron-based chelates for preventing Fe deficiency in the grapevine as well as moderating the different levels of iron chlorosis. This strategy could be an alternative to the use of synthetic Fe chelates as EDTA or o,o-EDDHA for preventing Fe chlorosis in susceptible Tempranillo (Vitis vinifera L.) vineyard in calcareous soils, with less of a risk to the environment.

Potential of functional analysis applied to Sentinel-2 time-series to assess relevant agronomic parameters at the within-field level in viticulture

• Functional analysis methods were able to decompose the yearly trend and spatial structure of the vineyards. • Performance of PCA declines if more than three componets are added. • The results are linked to soil characteristics and other key variables, highlighting several phenomena affecting the vineyard. Sentinel-2 satellite imagery offers a wealth of spectral information combined with a weekly temporal resolution. It is seen as a promising tool to extract spatial information about vineyards and link them to agronomic parameters. Usually, only one or a few images are commonly employed at specific stages like veraison in viticulture. Extracting further information from time-series images may be of interest; however, this remains an issue due to the noisy and complex nature of extracted time-series. The functional analysis proposes a robust continuous representation of these time-series, which can then be used with adapted statistical tools. This paper focuses on extracting relevant information at the within-field level on two vineyards in Spain, which can be jointly interpreted with field observations and measurements. More precisely, it discusses the use of popular linear dimensionality reduction techniques, namely Principal Component Analysis (PCA) and Partial Least Square (PLS), adapted to functional data in order to decompose NDVI time-series into a weighted sum of several functional components. The unsupervised methods, like PCA, decomposed the spatial structure within the vineyards using a few components, resulting in a better and more manageable dataset than the one obtained using simple non-constrained methods. The results show significant correlations with ground-truth data showing the added value of considering the whole NDVI temporal series compared to a single NDVI map at veraison. The proposed approach provided helpful information about each component's yearly trend. Moreover, the results are linked to grapevines' seasonal phenology and management practices, highlighting phenomena affecting the vineyard's development. This method is particularly suited for interactions with field experts, who may derive relevant agronomic information from the decomposition maps.

Vineyard modernization drives changes in bird and mammal occurrence in vineyard plots in dry farmland

The fast intensification of agriculture affects many systems. In recent decades, traditional vineyards have been rapidly converted to trellis vineyards in Spain (the country with the largest vineyard surface worldwide) in an attempt to reduce harvesting costs and increase vine productivity. The implications of this modernization of vineyards on farmland biodiversity are still largely unknown. We studied 52 vineyards (26 traditional and 26 trellis) from June to September in south-western Spain, aiming to describe the effect of the modernization of goblet-shape traditional vineyards on vine structure and their management, as well as its effect on species' occurrence in vineyard plots. We applied hierarchical occurrence models to assess the occurrence probability of 10 bird species and two mammal species in traditional and trellis vineyard plots. Vineyard modernization involved taller vines (connected by metallic guide wires and poles by rows), greater distance between vine rows, bigger plot sizes, the systematic implementation of irrigation, and an application of fertilizers through the watering system (fertirrigation) in a third of studied vineyards. Other agrochemical treatments seemed to be equally used in both vineyard types. Due to the use of herbicides and frequent tilling, both types of vineyards showed low natural vegetation groundcover. Vineyard modernization had clear effects on fauna biodiversity in vineyard plots, with a higher occurrence in trellis vineyards of some species (rufous-tailed scrub-robin, European goldfinch, red-legged partridge, and house sparrow) and a higher occurrence of other species (European rabbit and European greenfinch) in traditional ones. Thus, vineyard modernization can drive a change in the community of birds and mammals that inhabit them. In addition, vineyard use by some species (particularly ground-dwelling ones) was strongly determined by the presence of arable land adjacent to the vineyard. These species may therefore use vineyards during summer because they provide water and/or better cover than harvested crops. Animals attracted to vineyards for water could be exposed to toxic doses of nitrates that are routinely applied through the watering system in a third of these. To maximize benefits for biodiversity, it would be advisable to manage modernization schemes in order to maintain landscape heterogeneity, with vineyards of both types combined with other agricultural systems, as well as higher proportions of natural vegetation surface cover.

Evolution of fungicide residues in pruned vine-shoots

Pruned vine-shoots used as wine additives have proven to be a useful tool for improving and differentiating wines. This is because they accumulate substances from the plant itself, which later, as a result of the toasting process, express their greatest oenological potential. However, vine being one of the crops subject to the most phytosanitary treatments, including fungicides in particular, it can be assumed that their residues will accumulate in the vine-shoots and pass into the wine. The aim of this study was to determine the content in pruned vine-shoots of four of the main fungicides applied in the vineyard in Spain: trifloxystrobin, boscalid, kresoxim-methyl and penconazole. In order to do so, a HPLC-MS/MS method was developed, which showed high reliability given its adequate validation parameters. The method was linear for the concentration range studied, LOD was 0.003 mg/kg and LOQ was 0.01 mg/kg, which comply with the control criteria.The evolution of residues in vine-shoots complying with critical agricultural practices and good agricultural practices was monitored at 1, 3 and 6 months of storage after pruning and, in the latter case, after being subjected to a toasting process. The dissipation of all the residues was demonstrated to be affected by storage duration and toasting. It was confirmed that converting vine-shoots into oenological additives for wines will not pose a risk to the consumer if good agricultural practices are applied.

Different Ground Vegetation Cover Management Systems to Manage Cynodon dactylon in an Irrigated Vineyard

Ground cover management in vineyards in Spain is focused on minimizing soil erosion and compaction. Such practices have influenced the weed community structure in the inter-rows, contributing to the spread of the high noxious weed Cynodon dactylon (L.) Pers. This fact highlights the need for further investigation of the interaction between ground cover practices and weed control techniques. In this study, the effect of four different ground cover managements (M) in the inter-rows on C. dactylon population dynamics (changes in coverage and frequency) was assessed over three seasons (2015–2017): (M1) a no-till spontaneous vegetation ground cover managed by shredding; (M2) a no-till spontaneous vegetation ground cover managed by shredding plus herbicide application, (M3) tilled soil and spontaneous vegetation growing; and (M4) tilled soil and a barley cover crop seeded (Hordeum vulgare L.). Cynodon dactylon and the other weeds responded differently to the various weed control methods. After three seasons, the barley cover crop was the most efficient management system to control C. dactylon and other weeds. Final soil cover in barley cover crop and tilled soil with spontaneous vegetation were 0.5% and 1.1%, respectively, compared to 3.7% and 7.7% obtained by spontaneous vegetation shredded with and without herbicide application, respectively. In addition, total weed frequency varied from 9.7% for barley cover crop to 45.8% for spontaneous vegetation only shredded. Weed community composition changed due to the pressure exerted by each management and the adaptive strategy of the different species. This study highlights the importance of knowledge of how vegetation management influences weed flora to improve the sustainability of wine grape production systems.

Spread parameters of the borerXylotrechus Arvicola(Olivier) (Coleoptera: Cerambycidae) in a ‘Tempranillo’ vineyard in La Rioja (Spain): A long-term study

Xylotrechus arvicola(Olivier) (Coleoptera: Cerambycidae) is a polyphagous xylophagous beetle that is becoming a pest of increasing importance for vineyards in Spain, also because of the wood fungi developing in the galleries excavated by its larvae, which cause a progressive decline of the affected grapevines, until death. Between 1993 and 2015, a survey of the infestation caused byX. arvicolaand the symptoms caused by pathogenic wood fungi was performed in a ‘Tempranillo’ variety vineyard in La Rioja region (Spain). Maps showing the overtime spread of the borer and the diffusion of symptoms of grapevine decline andEutypadieback were obtained. Results indicated that the borer colonization began in the centre of the plot, followed by the first symptoms caused by the wood fungi a few years later. The statistical analysis showed that the evolution of infestation is characterized by a linear increase of new holes whereas the pattern of their allocation in the vines follows a bimodal distribution which, to some extent, can be simulated by a Poisson’s model. Based on these observations, a methodology to estimate the state of the infestation over time is proposed. The procedure - based on a linear regression of the average number of holes per vine over a set of years - can be applied in a relatively simple way and provides the probability for a grapevine to have a certain number of exit holes in a definite year with a mean error of around 5%.

Leafroller-induced phenylacetonitrile and acetic acid attract adult Lobesia botrana in European vineyards.

We recently identified unique caterpillar-induced plant volatile compounds emitted from apple leaves infested with the larvae of various leafroller species. In subsequent field tests, binary blends of phenylacetonitrile+acetic acid and 2-phenylethanol+acetic acid were found to be attractive to a range of tortricid leafroller species (Tortricidae: Tortricinae) in both the Southern and Northern Hemispheres. In this work, the caterpillar-induced plant volatiles from the apple-leafroller system were tested in two vineyards in Spain and Hungary for their attractiveness to the grape frugivore Lobesia botrana (Tortricidae: Olethreutinae). As seen for Tortricinae species, a binary blend of phenylacetonitrile+acetic acid attracted significantly more male and female L. botrana to traps than acetic acid or blank lures. Traps baited with other caterpillar-induced plant volatile compounds (benzyl alcohol, 2-phenylethanol, indole, and (E)-nerolidol, each as a binary blend with acetic acid) did not catch significantly more moths than traps containing acetic acid alone. The catches of male and female moths support an optimistic future for new products in female tortricid surveillance and control that are based on combinations of kairomone compounds released from larval-damaged foliage.

Improving stock unearthing method to measure soil erosion rates in vineyards

Vineyard soils experience high erosion rates compared to soils from other agricultural land uses. The high soil losses in vineyards limits the sustainability of traditional production schemes and warrants comprehensive research aimed at thwarting the main erosion processes affecting vineyard systems. However, long-term measurements, which include spatial variability of soil erosion rates at the plot scale, are uncommon, as most of the measurements have taken place either at the hillslope or watershed scales. Against this background, the stock unearthing method (SUM) can be considered a useful methodology. However, the current method falls short because it assumes that the topography between the vine lines (inter-rows) remains planar. Therefore, we propose a new methodology (ISUM: improved stock unearthing method) that includes three measurements between in the inter-row areas. By taking inter-row measurements, we hypothesized that the spatial patterns of sediment detachment, transport and deposition features in the inter-rows would be detected. The ISUM costs 20% more time to conduct than the SUM, but greatly improved the utility of the field survey. ISUM allowed for: i) the creation of maps that identified linear soil erosion features and accumulation sites; ii) measures the amount of soil accumulated under the vines; iii) estimation of soil erosion rates with higher accuracy at long-term periods in a specific moment. This study compared the ISUM with the SUM in a vineyard in Spain. Soil erosion rates with ISUM were −2.5 Mg ha yr−1 while the SUM calculated rates of +4.9 Mg ha yr−1.Results showed that the traditional method underestimated soil rates a −25.7%. Maps created with the ISUM technique could also be used to provide insights about sediment connectivity.

A territorial approach to assess the transition to trellis vineyards in special protection areas for steppe birds in Spain

Traditional vineyards in Spain are being upgraded to trellis vineyards. A key question is to what extent the transition to trellis vineyards is compatible with the environmental preservation goals in Special Protection Areas. This paper presents a GIS-based model to assess the suitability of Special Protection Areas for the development of trellis systems. The model was applied in a Special Protection Area for steppe birds in Castilla-La Mancha, Spain, a region with the largest concentration of vineyards in the world. The model includes three components: (1) a vineyard structure analysis, (2) an analysis of habitat fragmentation, and (3) a suitability analysis. The results showed that 50.9% of the study area, including 82.1% of the existing vineyards, was classified as suitable for transition to trellis systems. In the remaining unsuitable areas an increase in trellis systems should be avoided. The model is a stepping stone for improving the sustainable land use development of Mediterranean agricultural landscapes. Further research is needed to include wider socio-economic and environmental consequences of modernizing viticulture practices.

Soil microbiological properties and its stratification ratios for soil quality assessment under different cover crop management systems in a semiarid vineyard

AbstractIn vineyards in Spain, tillage and semiarid Mediterranean climatic conditions accelerate organic matter loss from the soil. Cover crops are a conservation management practice that can provoke changes in soil quality which requires evaluation. Stratification ratios of soil properties such as soil organic C and labile C fractions have been proposed for the assessment of soil quality under different soil management systems. Our objective was to study the effect of different cover crop management on various soil parameters and their stratification ratios. We evaluated three different soil managements in a Typic Haploxerept from NE Spain: conventional tillage (CT); 5‐y continuous cover crop of resident vegetation (RV); and 4‐y continuous cover crop of Festuca longifolia Thuill., followed by 1‐y Bromus catharticus L. after resowing (BV). We monitored soil organic C, particulate organic C, water soluble C, potentially mineralizable N, microbial biomass C, β‐glucosidase and urease enzymatic activities, and water stable aggregates at 0–2.5, 2.5–5, 5–15, 15–25, and 25–45 cm soil depths. We calculated soil depth stratification ratios of those soil properties. Resident cover crop increased microbiological properties, labile C fractions, and aggregation with respect to conventional tillage at 0–2.5 and 2.5–5 cm soil depths. However, for Bromus cover crop the same soil properties were lower than for the resident cover crop at 0–2.5 cm depth. Stratification ratios of β‐glucosidase and urease enzymatic activities, and particulate organic C showed a higher sensitivity than other soil properties; therefore, they would be the best indicators for soil quality assessment in semiarid Mediterranean vineyards.

A Wireless Sensor Network for Vineyard Monitoring That Uses Image Processing

The first step to detect when a vineyard has any type of deficiency, pest or disease is to observe its stems, its grapes and/or its leaves. To place a sensor in each leaf of every vineyard is obviously not feasible in terms of cost and deployment. We should thus look for new methods to detect these symptoms precisely and economically. In this paper, we present a wireless sensor network where each sensor node takes images from the field and internally uses image processing techniques to detect any unusual status in the leaves. This symptom could be caused by a deficiency, pest, disease or other harmful agent. When it is detected, the sensor node sends a message to a sink node through the wireless sensor network in order to notify the problem to the farmer. The wireless sensor uses the IEEE 802.11 a/b/g/n standard, which allows connections from large distances in open air. This paper describes the wireless sensor network design, the wireless sensor deployment, how the node processes the images in order to monitor the vineyard, and the sensor network traffic obtained from a test bed performed in a flat vineyard in Spain. Although the system is not able to distinguish between deficiency, pest, disease or other harmful agents, a symptoms image database and a neuronal network could be added in order learn from the experience and provide an accurate problem diagnosis.

Effects of the origins of Botrytis cinerea on earthy aromas from grape broth media further inoculated with Penicillium expansum

Earthy “off” aromas from wine and grape juice are highly detrimental to the production of quality grape products. These volatile compounds are produced on grapes by Botrytis cinerea, Penicillium expansum and/or a combination of P. expansum and B. cinerea strains. B. cinerea strains were isolated from different (a) vineyards in Spain and Portugal, (b) grape varieties (c) bunches (i.e., sound and botrytized) and (d) positions in the botrytized bunch (i.e., interior or exterior). A novel Headspace-Phase Microextraction (SPME) followed by Gas Chromatrography/Mass Spectrometry (GC–MS) dedicated to analyze geosmin, methylisoborneol (MIB), 1-octen-3-ol, fenchone and fenchol in grape broth medium was used. Approximately 50% of the B. cinerea strains induced detectable geosmin. One strain accumulated significant amounts of anisoles, demonstrating that this contamination might already occur in the vineyard. Strains from the interior of Cainho grape bunches induced more geosmin and hence it may be possible to reduce this volatile in wine by avoiding using these grapes in case of B. cinerea attack.

Interactive effects of deficit irrigation and shoot and cluster thinning on grapevine cv. Tempranillo. Water relations, vine performance and berry and wine composition

The effects of crop level and irrigation on water relations, yield, grape and wine composition were studied during two seasons in a Tempranillo vineyard in Spain. Irrigation was applied at two levels: R2 (with mild deficit irrigation applied during all the season) and R1 (with more severe water stress applied before veraison). Deficit irrigated vines were compared to a non-irrigated control. Crop levels imposed resulted in 11, 20, and 27 clusters per vine. Over all treatments, yield and ratio of leaf area to yield (LA: Y) were different between years: 4.4 and 16.3 t ha−1 and 1.72 and 0.88 m2 kg−1 in 2005 and 2006, respectively. In 2005, large differences in grape and wine composition occurred among non-irrigated and the irrigated treatments, but not between R1 and R2 treatments. Wines from non-irrigated vines were more acid, had higher total anthocyanins, and higher color intensity. In 2006, irrigation had less effect on grape and wine variables. The effect of shoot and cluster thinning on wine composition was different between seasons due to the different crop load values between years. Grape composition was negatively affected by high crop level only for values of LA: Y lower than 1.5 m2 kg−1.

Effects of mechanical thinning on fruit and wine composition and sensory attributes of Grenache and Tempranillo varieties (Vitis vinifera L.)

Background and Aims: Cost-effective yield control is required by the wine industry in order to reduce the existing worldwide wine surplus and to improve grape quality. The object of this study was to evaluate the chemical and sensory effects on the resulting wines of mechanical cluster thinning performed at different intensities on Grenache, and at different timings (pea size and veraison) on Tempranillo. Methods and Results: The experiments were conducted with a machine harvester in 2007 on two commercial vertical shoot-positioned vineyards in Spain's Rioja region. In both varieties, mechanical thinning was effective in yield reduction and resulted in more ripened fruit, and wines with higher alcohol and pH values, more intense colour and increased phenolic content. Regardless of the thinning intensity, sensory changes were less noticeable in Grenache than in Tempranillo wines. Of the latter, those from vines thinned during veraison were less aromatic and sour, but had increased astringency. Conclusions: Yield management through mechanical thinning induced changes to the chemical composition of fruit and wines as well as to the wines' aroma, taste and mouthfeel. The extent of the sensory implications seems to depend on several factors such as the variety and timing of thinning application. Significance of the Study: This is one of the first studies addressing the effects of mechanical thinning on the sensory properties of the resulting wine and has implications for viticultural management practices.

Effect ofXylotrechus arvicola(Olivier) (Coleoptera, Cerambycidae) Infestations on Flowering and Harvest in Spanish Vineyards

Some high-value vineyards in Spain are increasingly threatened by a xylophagous insect, <i>Xylotrechus arvicola</i> (Olivier), a polyphagous cerambycid that has become a grapevine pest during the last 10 years. Infestations are difficult to detect and control because the larvae excavate large and numerous galleries inside the wood. This study was performed during flowering and harvest time in La Rioja vineyards to evaluate the effects of infestations by <i>X. arvicola</i> on Tempranillo. Results showed that the inflorescence size and number of flowers were considerably reduced and that, during harvest, grape clusters were smaller and looser, weighing on average five times less than those collected from sound branches. Moreover, wine made from grapes on infested branches had significantly lower alcoholic percentage and higher organic acid concentration. These findings show that the damage caused by <i>X. arvicola</i> is severe both in terms of yield and wine composition. Increasing reports of its presence indicate that this serious insect pest be considered in integrated pest management of vineyards.

Occurrence of Fungal Pathogens Associated with Grapevine Nurseries and the Decline of Young Vines in Spain

AbstractThe occurrence of fungal grapevine trunk pathogens associated with grapevine nurseries and the decline of young vines in Spain was determined in extensive surveys conducted in nurseries and vineyards with young plants. The presence of Phaeomoniella chlamydospora, Phaeoacremonium aleophilum, Cylindrocarpon spp., Botryosphaeria obtusa and Botryosphaeria spp. was detected in all the surveys that were carried out. This study provides evidence for the presence of these pathogens in Spanish grapevine nurseries. Cylindrocarpon spp., B. obtusa and Botryosphaeria spp., were isolated very early in the planting material production process, nevertheless, P. aleophilum and P. chlamydospora were not detected until the rootstock–scion combinations were planted in the field to develop shoots and roots during summer. The occurrence of trunk disease pathogens in symptomatic young vineyards was also high, suggesting that infected plant material might have been used. Phaeomoniella chlamydospora and P. aleophilum were the main species associated with the decline of young vines, followed by Botryosphaeria spp., Cylindrocarpon spp. and B. obtusa. This research confirmed the importance of fungal grapevine trunk pathogens associated with the decline of young vineyards in Spain and suggested the connection between the presence of these fungi in the nurseries and the disease in the fields.