The aim of this study was to investigate impact of bio-humus on Cardinal grape yield (Vitis vinifera L.) and nutrient contents of dark brown soil using drip irrigation systems under the open field conditions in the vineyard region of Azerbaijan. A field experiment was conducted in a Cardinal Vineyard farm located in Shamakhi district, Azerbaijan between May and October 2021. An experiment with one dose of bio-humus treatment (5 t.ha-1) and three replications, with a plot size of 1 ha treatment was used. There were performed drip irrigation, starting from May 15 up to September 15, every 15 days. The soil sampling and measurements carried out after harvest the application of bio-humus in soil and the soil samples were collected from depth of 20 cm. The results showed that addition of bio-humus increased fresh berryweight yield, contents of organic matter, total N, available P, and available micronurtient (Fe, Cu, Zn and Mn) in soil compared with control plots. The soils treated with bio-humus had significantly more EC in comparison to unamended plots. The addition of bio-humus in soil resulted in increase of soil pH.