Vimba Vimba Research Articles

Improved innate immunity in juvenile vimba bream (Vimba vimba) fed a dry diet with an additive of hydrochloric acid (HCl)

Abstract A few reports indicate the beneficial effects of organic acids and their salts on anti-infective immunity in fish species. In the role of immunostimulants, inorganic acids may prove to be a much cheaper alternative to their organic equivalents. However, no report has described the effect of using inorganic acid as a feed additive on fish immunity. This study is the first attempt to evaluate the effect of hydrochloric acid (HCl) as a fish feed supplement on cellular immunity in the stomachless fish, cypriniform vimba bream, Vimba vimba (L.). Two groups, three replicates each, of juvenile vimba were fed a commercial dry fish diet or its variant containing a 1.5% additive of HCl for 55 days. The experiment was conducted in a recirculating aquaculture system at 25°C. Mortality, growth, condition factor, feed conversion ratio, respiratory burst activity (RBA), potential killing activity (PKA), and the proliferative response of head kidney lymphocytes were determined for both groups at the end of the experiment and compared. The PKA was higher in the group fed the diet with HCl. For all other parameters studied, there were no significant differences between the experimental groups. The results of the current study prove that the dietary additive of inorganic hydrochloric acid can substantially improve immune response to bacterial infections in juvenile vimba bream.

ALIEN VIMBA BREAM VIMBA VIMBA (CYPRINIDAE) IN THE URAL RIVER BASIN

This paper provides information on the presence of the vimba bream ( Vimba vimba ) in the middle reaches of the Ural River. One of the distinguishing features of this species from the native Caspian vimba ( V. persa ) is a greater number of scales in the lateral line. In the late 1980s, the vimba bream was introduced from the Don River basin into the Volga River basin. Having successfully adapted in the Volgograd reservoir, the species subsequently settled in the unregulated section of the Lower Volga, in the northern and, apparently, middle parts of the Caspian Sea, and also formed a stable residential population in the Ural River basin.

Artificial reproduction of vimba bream (Vimba vimba) cultured generation F2 and eggs management under controlled conditions

The experiments were conducted to investigate the hormonal stimulation of oocyte maturation in cultured (F2 generation) vimba bream (Vimba vimba) under controlled conditions and some aspects of egg management. In artificial reproduction, four hormonal agents (CPH, Ovopel, Ovaprim, and hCG) were tested. Fish were intraperitoneally (IP) injected with two doses of the hormonal agents at the base of the ventral fin. The highest percentage of ovulating vimba bream females (100%) was obtained in the Ovaprim group. Fertilization and hatchability percentages were nearly 95% and 93% in this group. In the control group, which was not injected with hormones, only one female from seven ovulated. Regarding the egg management aspects, the effects of 1) time adding sperm to activated eggs, 2) portional adding sperm to eggs, and 3) pH of the ovarian fluid on fertilization success of vimba bream were also assessed. The results indicate that vimba bream eggs retain fertilization capacity as long as 150–180 s after their activation. When fertilization was carried out within 30 s from the activation of the eggs, the hatchability rate of the larvae was over 80%. The analysis of the results of the present study indicates that there is a statistically significant correlation between vimba bream ovarian fluid pH and the hatching rate of larvae (R2 = 0.954). Moreover, a hatching rate (%) of >50% was noted in the females, of which the ovarian fluid pH was above 7.6. Thus, the results of the present study indicate that the pH of the ovarian fluid is an important factor affecting the quality of vimba bream gametes. The obtained results can be successfully used to produce vimba bream stocking material in a fish farm with the use of cultured spawners.

Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia.

In this study a battery of bioassays, both in vivo (metals and metalloids concentrations, erythrocyte morphometry, comet assay, micronucleus assay, and histopathological analyses) on vimba bream Vimba vimba (L., 1758) and white bream Blicca bjoerkna (L., 1758), and in vitro (treatment of HepG2 cells with native water samples) was applied to assess the harmful potential of untreated wastewater. Faecal indicator bacteria were quantified to assess the microbiological water quality. Vimba bream had significantly higher Fe concentrations in both liver and muscle, while white bream had higher Ca and Cu concentrations in liver. Vimba bream had a significantly higher level of DNA damage in both liver and blood cells, in comparison to white bream. Low levels of micronucleus and nuclear abnormalities were observed in both species. Erythrocytes morphometry did not show significant interspecific differences. Histopathological analyses revealed a similar response of the studied species, with a significantly higher presence of ceroid pigments in the liver of vimba bream. Treatment of HepG2 cells revealed the high genotoxic potential of water downstream of the discharge point. The results of this study clearly demonstrate the importance of effect-based monitoring, in order to enforce more efficient management of natural resources and implementation of wastewater treatment systems.

Bioaccumulation of potentially toxic elements in fish species of Serbia: a review.

In the first review of potentially toxic elements (PTEs) in fish in water ecosystems in Serbia, 40 studies related to fish contamination with PTEs were identified and evaluated. Studies of concentrations of PTEs in fish tissues covered 28 ecosystems (108 localities). The main studied river ecosystems were the Danube and the Sava, and the Međuvršje reservoir was the most studied standing water ecosystem. Among the most studied fish species were catfish (Silurus glanis), followed by pikeperch (Sander lucioperca), bream (Abramis brama), carp (Cyprinus carpio), and barbel (Barbus barbus). There were 48 "hot spot" sampling sites where concentrations of Hg, Cd, Pb, As, Ni, Fe, and/or Cu exceeded the maximum permitted concentrations (MPCs) prescribed by legislation. Elevated concentrations of these elements were the most detected in fish species sampled in the Danube, Sava, and rivers belonging to the Velika Morava basin. Hg concentrations were the most common above MPC in muscle tissue of species sampled from the Danube and Sava rivers. On the other hand, we can single out the species Vimba bream (Vimba vimba), sampled in Danube River in Zemun near Belgrade, as the safest species for human consumption due to the largest number of elements in fish meat below detection limits. Finally, having in mind all the criteria prescribed by U.S. EPA (2000), we identify and recommend two predatory fish species pikeperch (S. lucioperca) and catfish (S. glanis), and bottom-feeding fish species barbel (B. barbus) as bioindicators of PTEs contamination in Serbian waters.

Bisphenol A Used in Plastic Industry Negatively Affects Wild Vimba Bream (Vimba vimba)

Used as a component of polycarbonate plastics and epoxy resins in the modern industrial world, bisphenol A (BPA) is one of the highest volume produced industrial chemicals worldwide. BPA causes important environmental problems including endocrine disrupting effect on the organisms in aquatic environments. This study focused on the histopathological, oxidative and hematological effects of long-term BPA exposure on wild vimba bream (Vimba vimba) for the first time. Twenty-five fish stocked in each aquarium and exposed to 1000 μg/L BPA for four weeks. Hematological parameters of fish did not differ significantly compared to control group. The antioxidant activity of liver and gill tissues created a significant partial difference between the groups. According to the histopathological studies, intense hyperplasia and necrosis were detected in the gill tissues of the fish exposed BPA. In addition, hypertrophy and epithelial lifting symptom were partly observed. Vocalization and hypertrophic cells was determined in the liver tissues. In conclusions, this study revealed that BPA had an oxidative and especially histopathologically adverse effects on V. vimba, even though it did not have a hematological effect on the treated fish. Although BPA has adverse effects on the health of aquatic organisms, future studies should focus on the residue in fish meat and risk assessment on human health.

Comparison of different methods of wild vimba bream Vimba vimba (L.) spawning under controlled conditions

This paper describes a study in which different types of the reproduction of wild vimba bream (Vimba vimba) under controlled conditions were compared. Reproduction was performed by using three methods: spontaneous (natural spawning- NS groups): without hormones but with nests, semi-artificial (S groups) with hormones and nests, and artificial (A groups) with hormones and manual stripping of gametes. In groups S and A, different types of hormonal stimulation were tested: common carp pituitary homogenate (CPH), human chorionic gonadotropin (hCG), and commercial pellets containing mammalian GnRHa and dopamine antagonist-metoclopramide (Ovopel). The number of spawning fish, i.e., spermiating males and ovulating females, were compared depending on the type of breeding and the stimulant used. The lowest number of finally matured fish: spermiating males (67 %) and ovulating females (0 %) were noted in the spontaneously reproduced group. The best method of reproducing wild vimba bream under controlled conditions was the semi-artificial approach with hormonal stimulation and spawning nests, where ovulation rates in hormonally treated groups were between 43 % and 71 %, vs 26–57 % in artificial spawning groups. No ovulation observe in control groups in S and A types of reproduction. The highest embryo survival to the eyed-egg-stage was noted in group A-hCG (average 88.2). A higher value of spawning effectiveness (Se) with the use of the same hormones was obtained for groups S (0.374–0.574) than for groups A (0.252–0.493). In both cases, the highest Se was recorded for Ovopel stimulated fish and the lowest in hCG stimulated groups.

Ichthyofauna of the Bilotserkivskyi lower reservoir on the Ros river

The composition of ichthyofauna of Ukraine is changing due to natural processes and the influence of anthropogenic factors in the internal water bodies. Systematic analysis of changes allows controlling these processes and maintaining the diversity of species at a constant level. The purpose of the study was to investigate changes in the composition of the ichthyofauna of the Bilotserkivskyi lower reservoir (Ros River) after the discharge of industrial waste of the Bilotserkivvoda enterprise in 2017 and 2020, which led to the mass death of fish and crayfish. The studies were conducted by catching fish with species identification and a survey of fishermen from November 2016 to October 2020. Additionally, the species composition of fish in this section of the Ros River was considered before the reservoir was created. According to the results of the conducted studies, 24 fish species were identified in 2016, 21 in 2017, and 19 in 2020. Analysing the number of ichthyofauna since the 1960s, it can be concluded that every 10 years the species composition of the ichthyofauna of the Bilotserkivskyi lower reservoir lost about 2-3 species of fish, which is conditioned by the influence of riverbed overlap, industrial activities, uncontrolled and unidentified discharges into the reservoir, etc. After a detailed analysis of the number of ichthyofauna, it was established that by 2016, the following species had disappeared: common dace Leuciscus leuciscus, Dnieper chub P. borysthenicus, ide I. idus, sunbleak Leucaspius delineatus, vimba bream Vimba vimba, crucian carp Carassius carassius, weatherfish Misgurnus fossilis, spined loach Cobitis taenia; in 2017 – species such as gudgeon Gobio gobio, round goby Neogobius melanostomus, tubenose goby Proterorhinus marmoratus; in 2020 – char, ruffe. Quantitative and qualitative restoration of ichthyofauna is possible at the expense of fish from the Protoka River (Rotok). To continue fishing use, it is necessary to settle fish in the reservoir. The practical significance of this study is to determine the species composition of the ichthyofauna for further use of the reservoir for environmental and fishery purposes

Understanding Nutrition and Metabolism of Threatened, Data-Poor Rheophilic Fishes in Context of Riverine Stocking Success- Barbel as a Model for Major European Drainages?

Simple SummaryIt is important to consider the nutritional requirements and optimum feeding of hatchery-raised, conservation-priority rheophilic fishes meant for river stock enhancement. Properly selected diets for these nutritionally data-poor fish species are crucial to achieve optimum growth potential and physiology. It greatly reduces the time spent in captivity, and if properly trained, may produce fish that are better insured for facing hardships in the wild.Large-bodied, river-migrating, rheophilic fishes (cyprinids) such as barbel Barbus barbus, nase Chondrostoma nasus, asp Leuciscus aspius, and vimba bream Vimba vimba are threatened in major European drainages. This represents the subject of our present study. Their hatchery nutrition prior to river-release is mostly on a hit-and-trial or carp-based diet basis. The study demonstrates an alternative approach to decide optimum nutrition for these conservation-priority and nutritionally data-poor fishes. The study revealed barbel as a central representative species in terms of wild body composition among other native rheophilic cyprinids considered (asp, nase, vimba bream). Taking barbel as a model, the study shows that barbel or rheophilic cyprinids may have carnivorous-like metabolism and higher requirements of S-containing, aromatic, branched-chain amino acids (AAs) than carps. Besides, there are important interactions of AAs and fatty acids (FAs) biosynthesis to consider. Only proper feeding of nutritionally well-selected diets may contribute to river stocking mandates such as steepest growth trajectory (≈less time in captivity), ideal size-at-release, body fitness (≈blend-in with wild conspecifics, predator refuge), better gastrointestinal condition, maximized body reserves of functional nutrients, and retention efficiencies (≈uncompromised physiology). Considering important physiological functions and how AA–FA interactions shape them, hatchery-raised fishes on casually chosen diets may have high chances of physiological, morphological, and behavioral deficits (≈low post-stocking survivability). Based on the observations, optimum nutrient requirements of juvenile (0+ to 1+ age) barbels are suggested. Future efforts may consider barbels as a nutrition model for conservation aquaculture of threatened and data poor rheophilic cyprinids of the region.

Harvest Rates of Rheophilic Fish Vimba vimba, Chondrostoma nasus, and Barbus barbus Have a Strong Relationship with Restocking Rates and Harvest Rates of Their Predator Silurus glanis in Lowland Mesotrophic Rivers in Central Europe

The European catfish Silurus glanis (Linnaeus, 1758) is an expanding apex piscivorous predator whose predation may drive fish harvest rates and fish populations. This study aimed to analyze the relationships between intensive catfish stocking/harvesting and harvest rates of putative catfish prey–three rheophilic fish species: vimba bream Vimba vimba, nase Chondrostoma nasus, and barbel Barbus barbus (Linnaeus, 1758). The GAM (generalized additive model) was used to analyze the relationships between the harvest rate and the stocking intensity rate of the catfish and the three rheophilic fish species. The harvest rates and stocking intensity rates were obtained from mandatory angling logbooks collected from 38,000 individual recreational anglers by the Czech Fishing Union on 176 fishing sites over the years 2005–2017 in central Bohemia and Prague (the Czech Republic). Our results show that a higher intensity of catfish stocking and harvesting resulted in a lower harvest rate of rheophilic fishes. Conversely, the stocking rates of rheophilic fishes were not significantly correlated to their harvest rates. In conclusion, a significant negative relationship was found between the harvest rate and the restocking rates of rheophilic fishes and their predator, suggesting that fisheries managers should not perform intensive stocking of both catfish and rheophilic fishes on the same rivers.

Seasonal variations of macro-, micro-, and toxic elements in tissues of vimba bream (Vimba vimba) from the Danube River near Belgrade, Serbia.

Vimba bream (Vimba vimba) individuals were collected from the Danube River near Belgrade, from May 2016 to May 2017. Samples of muscle and liver tissue were analyzed for concentrations of 25 elements (macro-, micro-, and toxic elements) using inductively coupled plasma optical emission spectrometry (ICP-OES). For both tissues, the highest concentrations of most elements were observed in late spring/early summer (months of May and June). Majority of statistically significant correlations between elements were positive in both tissues. Contributions of Ca, K, and Mg to human diet were in a similar range. The lowest contribution of all macroelements was observed for Na, while the contribution of Se was well above the recommended dietary allowance for this element. Levels of detected elements were well below MAC values prescribed by national and international regulations. The highest value of Hg liver/muscle index (>1) was observed in August, which could indicate a higher contamination of the sampling location in that month. Se:Hg molar ratio never drops below 1, which could indicate that Hg toxicity is prevented. THQ values showed little variation thought the year and were well below the threshold of 1, with the exception of THQ for Al in May (0.92). This element also strongly affects the hazard index.

Gravel Quarries Could Affect Vimba Bream (Vimba vimba Linnaeus 1758) Populations from Someș River, Romania

The European stocks of vimba bream (Vimba vimba) are presently declining, having a "vulnerable" status in some countries. In Romania, there is a lack of scientific studies regarding the species. In order to supplement existing information about this species, specimens of vimba bream were collected from the middle sector of Someș River (Transylvania). Age, length and weight were recorded from 4 sampling points, near gravel quarries. The results show that only young specimens were found. The highest total length value was 22.77 cm, while the highest weight recorded was 121.08 g. Most vimba bream were captured in the first location, where less anthropogenic pressure exists. The populations are in decline in this region because of gravel quarries, among other factors.

Estimation of natural reproduction efficiency of semi-anadromous and river fish species in inland water bodies of the Republic of Dagestan, Russia

Aim. Estimation of the natural reproduction efficiency and patterns of formation of fish stocks.Material and Methods. Collection of material was carried out in 2010-2019 in inland water bodies in Dagestan and its shallow Caspian Sea coastal zone. Estimation of the number of juveniles in rivers and canals was carried out using ichthyoplankton cone seines. Fry seines were used to count juveniles of fish in water bodies and in the coastal area. 470 samples of juveniles were collected and 5640 juveniles were analysed. The collection and processing of materials were carried out according to generally accepted methods.Results. The main environmental factors determining the productivity of fish juveniles in the study area in the long-term have been identified. During the observation period, juvenile fish of 15 species were recorded in spawning grounds of inland water bodies. Roach, bream, carp, crucian carp, rudd and vimba bream dominate. In terms of numbers, roach juveniles predominate: their relative proportion being 31.02% with an increase from 27.1% in 2010 to 35.4% in 2018.The second place was occupied by bream juveniles, whose average relative proportion was 12.1%. The third place was occupied by carp juveniles with an average proportion of 9%. Juvenile zander, catfish, pike and kutum are relatively low in abundance from 2 to 4%. The yield of juvenile fish in 2010-2019 decreased 1.7 times compared to 1990-1995.Conclusion. It was established that a decrease in the number of juvenile fishes occurs under the influence of unfavorable environmental factors: decrease in sea level; reduction in spawning areas; the shallowing and silting of migration routes due to the lack of a stable water supply. Measures are proposed to improve the living conditions of aquatic biological resources, aimed at increasing the efficiency of their natural reproduction.

The Distribution of Vimba Bream Vimba vimba vimba (Actinopterygii: Cyprinidae) in the Unregulated Section of the Lower Volga River

This paper presents recent data on the population of the anadromous vimba bream (Vimba vimba vimba), introduced in the Volga basin. After penetration to the downstream section of the Volga River from the Volgograd Reservoir, vimba bream can be found throughout the entire unregulated stretch downstream of the Volga Hydroelectric Station. Observations show that the population size changes naturally during the year owing to its spawning migration. At the same time, a gradual transformation of the seasonal dynamics of the species is observed. As populations spread further downstream, the migrations of spawners downstream of the dam become longer, and the population density decreases. Also a seasonal redistribution takes place, with reduced migration observed in autumn and increased migration during spring. Meanwhile, the growth rate in the downstream section of the Volga River is comparable to the growth rate of populations in the Volgograd and Tsimlyansk reservoirs. Spawning occurs on in-channel spawning grounds. Yearlings are observed over a distance of 100 km downstream of the dam. In addition to Vimba bream, single specimens of Caspian bream (V. v. persa) are also recorded.

Evidence of the occurrence and reproduction of vimba bream (Vimba vimba) in the Nida River system

Abstract Once abundant in the Nida River, vimba bream (Vimba vimba) was not detected during extensive electrofishing surveys undertaken in the late 1960s, 1990s, or early 2010s. Irrespective, a few angler records of this species suggested that a small population of vimba bream might persist in the Nida River. Finally, several records from 2015, 2016, and 2017 confirmed its presence in this river system and indicated its successful reproduction in the middle reach of the river.

Ontogenic development of digestive accessory glands in larval and juvenile Vimba bream, Vimba vimba (Pallas, 1814)

The current research investigated histological development and histochemistry of digestive accessory glands of Vimba vimba from hatching through 60th day after hatching (DAH). The samples were randomly taken from day 1 (hatching) until the yolksac was absorbed (day 7) and during following day, on a daily basis, then with 2, 5, and 10 days intervals until 60 DAH. Histological procedures including fixation, tissue processing, sectioning‌, general staining such as Hematoxiline-Eosine and specific staining such as Periodic-Acid Schiff (PAS), were carried out . A primordial liver was observed at 1 DAH in the ventral region of the yolksac. The pancreas was observed at 2 DAH, and gall bladder and bile duct were developed at 4-5 DAH. At the same time when larvae were growing, digestive accessory glands continued differentiation and, at 5 DAH, hepatocytes of liver with eosinophilic cytoplasm and basophilic granules showed regular shape around Sinusoids. Coinciding with the liver development, sinusoids developed as well and hepatic cells increased in size. Sinusoids store large amount of blood to provide the necessary conditions for liver metabolism. Also, at this stage, eosinophilic zymogen granules of exocrine portion of pancreas were observed. Secretory activity of digestive accessory glands began with the emergence of PAS positive cells followed by secretion of neutral mucopolysaccharides and glycogen compounds in liver cells of larvae at 4 DAH and pancreatic cells of larvae at 4-7 DAH. In general, the results of this study can be used to identify the factors limiting larval growth, reducing casualties during exogenous feeding, and even considering a proper diet for digestive system.

Application of Different Classification Methods to Determine the Developmental Stage of Fish Erythrocytes of the Common Nase (Chondrostoma nasus) and Vimba Bream (Vimba vimba)

Fish erythrocytes released from erythropoietic sites are immature. During circulation, they increase their own surface, while the cell body and nuclei begin to become more elliptical and less spherical during maturation. The relative abundance of different developmental stages represents an erythron profile which could be a more sensitive indicator of contamination than classic hematological indices. In this study, we analyzed blood smears of the common nase (Chondrostoma nasus) and vimba bream (Vimba vimba) with the main goal to identify the determinants of the developmental stage of erythrocytes. Based on parameters developmental stages, erythrocytes are categorized into immature, intermediate or mature. In this investigation we have used four classification methods: the Two Step Cluster analysis, the K-Means Cluster analysis, and Neural Networks–Multilayer Perceptron and an Ordinal Regression model. Our findings clearly justify that Multilayers Perceptron and OLR models are appropriate to classify the developmental stage of fish erythrocytes in both species.

The effect of fishing effort, fish stocking, and population density of overwintering cormorants on the harvest and recapture rates of three rheophilic fish species in central Europe

Rheophilic fish species are among the most threatened groups of organisms. There is evidence that anthropogenic activities, such as fish stocking and fishing pressure, could have a significantly negative effect on freshwater fish populations. In addition to this, high population densities of cormorants, Phalacrocorax carbo, (one of the most important avian piscivorous predators in Europe) are also partially driven by anthropogenic activities. The first goal of this study was to estimate the effects of fishery management on fish harvest in three rheophilic fish species: barbel (Barbus barbus), nase (Chondrostoma nasus), and vimba bream (Vimba vimba). The second goal was to estimate the effect of cormorant population density on the harvest of these species. Individual mandatory angling logbooks data were collected on the 22 largest rivers in central Bohemia (Czechia, central Europe) over 13 years. Cormorant density was estimated using bird census data while the diet composition of cormorants was assessed using an analysis of fish diagnostic bones extracted from regurgitated bird pellets. In total, anglers visited the selected fishing areas 3 million times over 13 years and harvested 1 000 tons of fish, of which 4.5 tons were rheophilic species. The fishing effort was the most important driver of fish harvest. Fish stocking and cormorant population density did not significantly affect the fish harvest. However, rheophilic fish species comprised 8.8 % of fish consumed by cormorants (by biomass). In conclusion, fishing pressure was the most important factor affecting the harvest of rheophilic fish. Conversely, the intensity of predation by cormorants was not significantly affecting fish harvest despite their consumption of rheophilic fish. However, there is a possibility that predation pressure of cormorants was evenly distributed among fishing areas.

Monitoring spawning migrations of potamodromous fish species via eDNA

Potamodromous fish are considered important indicators of habitat connectivity in freshwater ecosystems, but they are globally threatened by anthropogenic impacts. Hence, non-invasive techniques are necessary for monitoring during spawning migrations. The use of environmental DNA (eDNA) potentially facilitates these efforts, albeit quantitative examinations of spawning migrations remain so far mostly uncharted. Here, we investigated spawning migrations of Danube bleak, Alburnus mento, and Vimba bream, Vimba vimba, and found a strong correlation between daily visual fish counts and downstream eDNA signals obtained from filtered water samples analysed with digital PCR and end-point PCR coupled with capillary electrophoresis. By accounting for daily discharge fluctuations, it was possible to predict eDNA signal strength from the number of migrating fish: first, the whole spawning reach was taken into account. Second, the model was validated using eDNA signals and fish counts obtained from the upper half of the examined river stretch. Consequently, fish counts and their day-to-day changes could be described via an eDNA-based time series model for the whole migration period. Our findings highlight the capability of eDNA beyond delivering simple presence/absence data towards efficient and informative monitoring of highly dynamic aquatic processes such as spawning migrations of potamodromous fish species.

Metal Accumulation in Muscle and Liver of the Common Nase (Chondrostoma nasus) and Vimba Bream (Vimbavimba) from the Danube River, Serbia: Bioindicative Aspects.

Accumulation of 17 elements in muscle and liver of common nase and vimba bream, caught between February and May 2016 in the Danube River (1173 river kilometer), were assessed by ICP-OES. The principal component analysis grouped muscle and liver samples based on element concentrations (muscle grouped by higher Ba and Sr values, and liver grouped by higher Al, B, Cd, Cr, Cu, Fe, Mn, and Zn values), but no grouping between the two species was observed. Concentrations of Ba, Cu, Fe, and Zn were significantly higher in muscle, and concentrations of Ba, Cd, Cu, and Mn in liver of common nase, while vimba bream had significantly higher concentrations of Cr and Fe in liver. Common nase has a higher affinity for bioaccumulation of Cu, Fe, and Zn in muscle, while vimba bream has a higher affinity for Al, Cd, and Cr in muscle.