Villages In Mali Research Articles

Malaria asymptomatic carriage in the Sahelian region: the challenge associated to young adults

Abstract Background Mali and Senegal have made important efforts to control malaria. After a period of decline, the number of cases has remained stable for several years. Asymptomatic carriage acting as a reservoir could explain malaria persistence despite malaria control. Mass drug administration (MDA) is a candidate intervention that could help to accelerate the decreasing of malaria transmission if favourable epidemiologic context and good adherence are met. We aimed to update asymptomatic reservoir description along a Sahelian ecological gradient and study MDA acceptability. Methods We conducted an open cohort in 8 villages in Mali and Senegal including all household members sampled aged >6 months. We measured P. falciparum infections prevalence by qPCR in 4 surveys: 2 in dry season 2021, 1 in rainy season 2021 and 1 in dry season 2022. We measured prospective acceptability of MDA in cohort aged ≥15 years in Senegal by questionnaire. We explored risk factors associated to asymptomatic infections and acceptability score with multilevel logistic and linear models. Results We included 168 households and 1428 participants in at least 1 survey. In preliminary results, prevalence of P. falciparum infections was 11% in dry season 2021 in Senegal, 20% in central Mali and 60% in Northern Mali. Young adults between 15 to 24 years old were more likely to carry asymptomatic infections in dry season compared to children <5 years old (OR = 5.74 [2.99;11.04]). MDA presented a good acceptability with a mean score of 3.5 (score between -7 and 7). Young adults had lower acceptability compared to older participants (β=−1.07 [-1.82,-0.32]). Conclusions Young adults were important asymptomatic carriers in Senegal and Mali but were less likely to accept to participate in MDA. Key messages • Young adults represent a public health challenge that need to be address to reach malaria elimination. • Specific sensibilization could be implement to improve adherence to malaria control interventions.

Isotopic evidence that aestivation allows malaria mosquitoes to persist through the dry season in the Sahel.

Data suggest that the malaria vector mosquito Anopheles coluzzii persists during the dry season in the Sahel through a dormancy mechanism known as aestivation; however, the contribution of aestivation compared with alternative strategies such as migration is unknown. Here we marked larval Anopheles mosquitoes in two Sahelian villages in Mali using deuterium (2H) to assess the contribution of aestivation to persistence of mosquitoes through the seven-month dry season. After an initial enrichment period, 33% of An. coluzzii mosquitoes were strongly marked. Seven months following enrichment, multiple analysis methods supported the ongoing presence of marked mosquitoes, compatible with the prediction that the fraction of marked mosquitoes should remain stable throughout the dry season if local aestivation is occurring. The results suggest that aestivation is a major persistence mechanism of An. coluzzii in the Sahel, contributing at least 20% of the adults at the onset of rains. This persistence strategy could influence mosquito control and malaria elimination campaigns.

Comparison of Different Sampling Methods to Catch Lymphatic Filariasis Vectors in a Sudan Savannah Area of Mali.

There is a need for better tools to monitor the transmission of lymphatic filariasis and malaria in areas undergoing interventions to interrupt transmission. Therefore, mosquito collection methods other than human landing catch (HLC) are needed. This study aimed to compare the Ifakara tent trap type C (ITTC) and the Biogents sentinel trap (BGST) to the HLC in areas with different vector densities. Mosquitoes were collected in two villages in Mali from July to December in 2011 and 2012. The three methods were implemented at each site with one ITTC, one BGST, and one HLC unit that consisted of one room with two collectors-one indoor and the other outdoor. The Anopheles collected in 2011 were individually dissected, whereas those from 2012 were screened in pools using reverse transcription-polymerase chain reaction (RT-PCR) to determine the maximum infection prevalence likelihood (MIPL) for Wuchereria bancrofti and Plasmodium falciparum. The dissection of the females also allowed to assess the parity rates, as well its results. Over the 2 years, the HLC method collected 1,019 Anopheles, yields that were 34- and 1.5-fold higher than those with the BGST and ITTC, respectively. None of the dissected Anopheles were infected. The RT-PCR results showed comparable MIPL between HLC and ITTC for W. bancrofti with one infected pool from each trap's yield (respectively 0.03% [0.0009-0.2%] and 0.04% [0.001-0.2%]). For P. falciparum, no infected pool was recovered from BGST. The ITTC is a good alternative to HLC for xenomonitoring of program activities.

Малийские детские деревни: социально-педагогическая среда для социализации детей-сирот

The article focuses on Sos Children’s Villages in Mali (West Africa), a country that has been at war since 2012. The war has killed hundreds of thousands of civilians and soldiers. This study is based on the current situation in the country, where the number of orphans in children’s villages has increased. It causes difficulties in providing social and educational support in children’s villages. However, despite the difficult conditions, teachers have been able to achieve results. How do they do it? What social and educational environments do they work in? What strategies are used to socialize orphaned children? The aim of the article is to describe the social and educational environment in which orphans in Mali children’s villages are socialized.

Video on Mobile Phones as an Effective Way to Promote Sustainable Practices by Facilitating Innovation Uptake in Mali

A challenge for researchers and other developers of new technologies in agriculture is to find ways of communicating their results and recommendations. This challenge is particularly acute in regions in which farmers have limited access to education and where illiteracy is widespread, such as in the rural areas of Mali. One approach that shows potential, yet remains largely unused by extension services, is the dissemination of educational videos on mobile phones with video and Bluetooth technology (3G mobile phones). It has been shown that the conditions are suitable for this approach to be used in Mali, with 3G mobile phones widely available in the region but there is little empirical evidence of its acceptance and potential impact. This article aims to explore the potential of video on mobile phones as a tool to facilitate the diffusion of new agricultural technologies in Western Africa. Three videos showing agricultural innovations for sustainable production were shown and shared with 200 farmers in twelve villages in Mali. The villages were revisited 10 months later and farmers were asked about their experiences with the videos that had been shared and their previous knowledge of the innovations shown in them. Of the farmers who had watched one of the videos (N=148), 60.1% had adopted at least one of the videos’ innovations. Mobile-phone videos could be accessed by people who had previously received limited access to information sources, such as younger women, and video based information was found to be understandable for illiterate farmers. These results show that mobile-phone videos could enhance information transfer and thereby expand outreach. The use of video on mobile phones is a novel approach to farmer-to-farmer exchange and has tremendous potential for enhancing dissemination programs or specific research and development projects to enable more resilient, inclusive and democratic systems.

Factors Associated with Wuchereria bancrofti Microfilaremia in an Endemic Area of Mali.

Although Wuchereria bancrofti (Wb), the causative agent of lymphatic filariasis, is endemic throughout Mali, the prevalence of Wb microfilaremia (Mf) can vary widely between villages despite similar prevalence of infection as assessed by circulating antigen. To examine this variation, cross-sectional data obtained during screening prior to an interventional study in two neighboring villages in Mali were analyzed. The overall prevalence of Wb, as assessed by Wb CAg (circulating antigen), was 50.3% among 373 participants, aged 14-65. Wuchereria bancrofti Mf-positive and negative individuals appeared randomly distributed across the two villages (Moran's I spatial statistic = -0.01, Z score =0.1, P>0.05). Among the 187 subjects positive for Wb CAg, 117 (62.5%) had detectable Mansonella perstans microfilaremia (Mp Mf) and 64 (34.2%) had detectable Wb microfilaremia. The prevalence of Mp microfilaremia was 73.4% in the Wb Mf-positive group (as compared to 56.9% in the Wb Mf-negative group; p=0.01), and median Wb Mf load was increased in co-infected subjects (267Mf/ml vs 100 Mf/ml; p<0.001). In multivariate analysis, village of residence, Mp Mf positivity and gender were significantly associated with Wb Mf positivity. After controlling for age, gender, and village of residence, the odds of being Wb Mf positive was 2.67 times higher in Mp positive individuals (95% confidence interval [1.42-5.01]). Given the geographical overlap between Mp and Wb in Africa, a better understanding of the distribution and prevalence of Mp could assist national lymphatic filariasis control programs in predicting areas of high Wb Mf prevalence that may require closer surveillance.

“An Be Jigi”: Collective cooking, whole grains, and technology transfer in Mali

This paper addresses how available resources, food security, technology, and culture are shaping the choices rural Malian women are making to ensure the health, energy, and well-being of their families. This research contributed to evaluating an eight-year research project (An Be Jigi) targeting improved nutrition. The study, performed over four months, used semi-structured interviews of 120 women in six villages in Mali to assess the identified issues with qualitative and quantitative approaches. This paper describes the history of the An Be Jigi project, whole-grain processing techniques, and group cooking for knowledge sharing with rural women for improved nutrition. Interviews revealed substantial adoption of whole-grain processing techniques and women’s appreciation of the nutritional benefits of those techniques. The women engaged in group cooking ( cuisines collectives) appreciated the activities and mentioned multiple benefits from using them. Women identified access to mills, and to some extent the social stigma of laziness and poverty associated with whole-grain food, as limiting factors of adoption. This study of women’s practices and perceptions regarding use of whole grain tells a story of changing consumption habits being shaped by culture, technology, knowledge, and available resources. Malian women are agents of change and care in their adoption of new techniques and recipes for the improved nutrition of young children and households.

The invasive shrub Prosopis juliflora enhances the malaria parasite transmission capacity of Anopheles mosquitoes: a habitat manipulation experiment

BackgroundA neglected aspect of alien invasive plant species is their influence on mosquito vector ecology and malaria transmission. Invasive plants that are highly attractive to Anopheles mosquitoes provide them with sugar that is critical to their survival. The effect on Anopheles mosquito populations was examined through a habitat manipulation experiment that removed the flowering branches of highly attractive Prosopis juliflora from selected villages in Mali, West Africa.MethodsNine villages in the Bandiagara district of Mali were selected, six with flowering Prosopis juliflora, and three without. CDC-UV light traps were used to monitor their Anopheles spp. vector populations, and recorded their species composition, population size, age structure, and sugar feeding status. After 8 days, all of the flowering branches were removed from three villages and trap catches were analysed again.ResultsVillages where flowering branches of the invasive shrub Prosopis juliflora were removed experienced a threefold drop in the older more dangerous Anopheles females. Population density dropped by 69.4% and the species composition shifted from being a mix of three species of the Anopheles gambiae complex to one dominated by Anopheles coluzzii. The proportion of sugar fed females dropped from 73 to 15% and males from 77 to 10%.ConclusionsThis study demonstrates how an invasive plant shrub promotes the malaria parasite transmission capacity of African malaria vector mosquitoes. Proper management of invasive plants could potentially reduce mosquito populations and malaria transmission.

Safety and efficacy of PfSPZ Vaccine against Plasmodium falciparum via direct venous inoculation in healthy malaria-exposed adults in Mali: a randomised, double-blind phase 1 trial

Plasmodium falciparum sporozite (PfSPZ) Vaccine is a metabolically active, non-replicating, whole malaria sporozoite vaccine that has been reported to be safe and protective against P falciparum controlled human malaria infection in malaria-naive individuals. We aimed to assess the safety and protective efficacy of PfSPZ Vaccine against naturally acquired P falciparum in malaria-experienced adults in Mali. After an open-label dose-escalation study in a pilot safety cohort, we did a double-blind, randomised, placebo-controlled trial based in Donéguébougou and surrounding villages in Mali. We recruited 18-35-year-old healthy adults who were randomly assigned (1:1) in a double-blind manner, with stratification by village and block randomisation, to receive either five doses of 2·7 × 105 PfSPZ or normal saline at days 0, 28, 56, 84, and 140 during the dry season (January to July inclusive). Participants and investigators were masked to group assignments, which were unmasked at the final study visit, 6 months after receipt of the last vaccination. Participants received combined artemether and lumefantrine (four tablets, each containing 20 mg artemether and 120 mg lumefantrine, given twice per day over 3 days for a total of six doses) to eliminate P falciparum before the first and last vaccinations. We collected blood smears every 2 weeks and during any illness for 24 weeks after the fifth vaccination. The primary outcome was the safety and tolerability of the vaccine, assessed as local and systemic reactogenicity and adverse events. The sample size was calculated for the exploratory efficacy endpoint of time to first P falciparum infection beginning 28 days after the fifth vaccination. The safety analysis included all participants who received at least one dose of investigational product, whereas the efficacy analyses included only participants who received all five vaccinations. This trial is registered at ClinicalTrials.gov, number NCT01988636. Between Jan 18 and Feb 24, 2014, we enrolled 93 participants into the main study cohort with 46 participants assigned PfSPZ Vaccine and 47 assigned placebo, all of whom were evaluable for safety. We detected no significant differences in local or systemic adverse events or laboratory abnormalities between the PfSPZ Vaccine and placebo groups, and only grade 1 (mild) local or systemic adverse events occurred in both groups. The most common solicited systemic adverse event in the vaccine and placebo groups was headache (three [7%] people in the vaccine group vs four [9%] in the placebo group) followed by fatigue (one [2%] person in the placebo group), fever (one [2%] person in the placebo group), and myalgia (one [2%] person in each group). The exploratory efficacy analysis included 41 participants from the vaccine group and 40 from the placebo group. Of these participants, 37 (93%) from the placebo group and 27 (66%) from the vaccine group developed P falciparum infection. The hazard ratio for vaccine efficacy was 0·517 (95% CI 0·313-0·856) by time-to-infection analysis (log-rank p=0·01), and 0·712 (0·528-0·918) by proportional analysis (p=0·006). PfSPZ Vaccine was well tolerated and safe. PfSPZ Vaccine showed significant protection in African adults against P falciparum infection throughout an entire malaria season. US National Institutes of Health Intramural Research Program, Sanaria.

Spatial Patterns of Plasmodium falciparum Clinical Incidence, Asymptomatic Parasite Carriage and Anopheles Density in Two Villages in Mali.

Heterogeneity in malaria exposure is most readily recognized in areas with low-transmission patterns. By comparison, little research has been done on spatial patterns in malaria exposure in high-endemic settings. We determined the spatial clustering of clinical malaria incidence, asymptomatic parasite carriage, and Anopheles density in two villages in Mali exposed to low- and mesoendemic-malaria transmission. In the two study areas that were < 1 km(2) in size, we observed evidence for spatial clustering of Anopheles densities or malaria parasite carriage during the dry season. Anopheles density and malaria prevalence appeared associated in some of our detected hotspots. However, many households with high parasite prevalence or high Anopheles densities were located outside the identified hotspots. Our findings indicate that within small villages exposed to low- or mesoendemic-malaria transmission, spatial patterns in mosquito densities and parasite carriage are best detected in the dry season. Considering the high prevalence of parasite carriage outside detected hotspots, the suitability of the area for targeting control efforts to households or areas of more intense malaria transmission may be limited.

The Role of Livestock Mobility in the Livelihood Strategies of Rural Peoples in Semi-Arid West Africa

Over the past 10 years, mobility of livestock has been portrayed as increasing the resilience of rural households in semi-arid Africa to climate change and variability. With this recognition, there has been important work characterizing livestock mobility and the barriers to it. This paper adds to this work by addressing two gaps in the literature: 1. An understanding in the variation of livestock mobility practices among communities; and 2. An understanding of rural peoples’ views of the advantages and disadvantages of livestock mobility as well as the factors affecting their decisions about herd movements. A mixed-methods approach was adopted to analyze data collected by household survey and group interviews conducted in 32 multi-ethnic villages in Mali and Niger spanning the 12.5° N to 16.5° N latitudinal range. The results of regression and qualitative analyses show that: 1. A large fraction of rural households rely on livestock as part of their livelihood strategies; 2. Grazing management of a large majority of village livestock depends on movements outside of the village territory, especially during the rainy season; 3. The mobility of village livestock is not strongly influenced by the village’s sociprofessional composition (farmer, herder, fisher, artisan..etc.); and 4. The prevalence of extra-village movements of village livestock (sheep and cattle) is higher in areas of higher population density. Despite the advantages of livestock mobility cited by informants, longer-distance movements are inhibited by risks associated with climatic, land-use, and sociopolitical change. Herd managers make decisions using diverse information about potential destinations with greater trust of information gathered by themselves or close kin. The implications of these findings for livestock management and policy in the region are discussed.

Sustainable Integrated Malaria Management by Villagers in Collaboration with a Transformed Classroom Using the Holistic Process: Sanambele, Mali, and Montana State University, U.S.A.

Pesticides, bed nets, and malaria medications are not affordable to farmers in remote villages in Mali and other material-resource poor countries. When provided without cost, preventatives often do not reach remote villages. If they do, the rationale for their use is not fully understood by villagers. In a single village, authors explore success and failure of top-down or "outside" approaches. The hypothesis tested was: sustainable malaria management can be entirely village-based after interaction and dialogue with a collaborating, transformed, undergraduate or graduate classroom using the holistic process, village homestays, and life cycle storytelling. This integrated approach combined: economic stability for women from a handicraft enterprise; physical larval management; community awareness; outreach to village school and neighboring villages; and a sustainable, village-made larvicide from neem (Azadirachta indica A. Juss.) leaf slurry against malaria (anopheline mosquito) vectors. Storytelling was the medium used to transfer knowledge. Patience, active listening, rapid-response literature searches, cell phone-initiated sampling, and creative thinking were skills practiced by 137 Montana State University (MSU) students in transformed classrooms collaborating with villagers via a specially trained mentor system of 6 Malian scientists and an environmental engineer. The holistic process was initiated in 2005. Economic interventions and home stays in the village began in 2007. Community awareness, physical management of larvae, and storytelling began in 2008. Deaths in Sanambele during 2008 from cerebral malaria (all children 0-5 years) decreased compared to previous years, despite a national cerebral malaria outbreak in Mali. Dry season larval mosquito management began in March 2009. There were no deaths from malaria in 2009 and afterward. Neighbor village outreach began in 2010. Community awareness involved villages' formal and informal educational systems. Villagers used their available resources to manage mosquito larvae sustainably, significantly reduced the incidence of malaria, and began transferring this information to neighboring villages.

Potential Use of Neem Leaf Slurry as a Sustainable Dry Season Management Strategy to Control the Malaria Vector &lt;I&gt;Anopheles gambiae&lt;/I&gt; (Diptera: Culicidae) in West African Villages

Larval management of the malaria vector, Anopheles gambiae Giles s.s., has been successful in reducing disease transmission. However, pesticides are not affordable to farmers in remote villages in Mali, and in other material resource poor countries. Insect resistance to insecticides and nontarget toxicity pose additional problems. Neem (Azadirachta indica A. Juss) is a tree with many beneficial, insect bioactive compounds, such as azadirachtin. We tested the hypothesis that neem leaf slurry is a sustainable, natural product, anopheline larvicide. A field study conducted in Sanambele (Mali) in 2010 demonstrated neem leaf slurry can work with only the available tools and resources in the village. Laboratory bioassays were conducted with third instar An. gambiae and village methods were used to prepare the leaf slurry. Experimental concentration ranges were 1,061-21,224 mg/L pulverized neem leaves in distilled water. The 50 and 90% lethal concentrations at 72 h were 8,825 mg/L and 15,212 mg/L, respectively. LC concentrations were higher than for other parts of the neem tree when compared with previous published studies because leaf slurry preparation was simplified by omitting removal of fibrous plant tissue. Using storytelling as a medium of knowledge transfer, villagers combined available resources to manage anopheline larvae. Preparation of neem leaf slurries is a sustainable approach which allows villagers to proactively reduce mosquito larval density within their community as part of an integrated management system.

“Copy Debts”?: —Towards a Cultural Model for Researchers’ Accountability in an Age of Web Democracy

“Copy Debts”? —Towards a Cultural Model for Researchers’ Accountability in an Age of Web Democracy Jan Jansen (bio) Introduction1 The highly standardized oral narrative about Sunjata, nowadays known as the Sunjata Epic, has been governing society since—at least—the fourteenth century when Arab travel writer Ibn Battuta on a trip along the Niger River reported a Sunjata tradition. This epic tells about the foundation of society—called “Mali” or “Mande”—and expresses values that go beyond the borders of countries: it explains the relationships among clans. It also prescribes how, based on patronymics and clan-related praise songs, every person should behave in public. The epic is also now much esteemed as Mali and Guinea’s medieval history and as a national and supranational charter, maintaining prominence both in the mass media and in educational programs (cf. Bulman 2004; Adejunmobi 2011). The name “Mali” itself, which in 1960 became the official designation for the territory, is definitely the most striking example of this heightened status of the Sunjata Epic.2 Several villages in Mali and Guinea have families living there that have much prestige because of their knowledge of the Sunjata Epic. In Mali, the Diabate family from Kela are among the most authoritative interpreters of the Sunjata Epic (cf. Austen 1999; Jansen 2001). I use the case presented in this article—about a Sunjata Epic recording in Kela and the discussions of ownership that the recording raised—to argue that researchers whose work deals with such an intangible heritage may have to reposition themselves. They must work from a radically different perspective than the one behind the usual discourse, which is based on concepts of permission/approval, individual author rights, and informed consent. A new attitude, based on the idea of “copy debts,” may meet the local deep concerns and unexpected claims that underlie a prestigious and intangible heritage. It is the property rights of this intangible heritage that form the main focus of this article. Any attempt to publish an oral tradition is a political statement in line with supporting an open society and an agenda of web democracy. One may even argue that researchers, in their academic attempt to maximize access to oral traditions and in their professional search and fascination for optimal recording and documentation technologies, are tempted to practice a “WikiLeaks mentality” by making accessible as much data as possible. However, the approach of researchers differs from WikiLeaks practices in the fact that researchers are concerned with the rights of their sources. The accountability of researchers in their desire to combine web democracy with a concern for local population groups will therefore be a central concern throughout this article, and the present-day concern for intellectual property rights provides its context. These rights are conceptualized in terms of copyrights and author rights (droits d’auteur). UNESCO’s program for Masterpieces of Oral Intangible Heritage of Humanity has definitely increased the focus on these rights and has institutionalized them. Unfortunately, UNESCO has institutionalized these rights in a primarily legalistic way, thus conceptualizing copyrights primarily from a written text perspective that prioritizes national copyright laws along with the idea that a group is, legally, a collection of individuals.3 I will argue here that it may be problematic and even undesirable for a fieldworker, who is closely related to the performers and the recording of the oral traditions, to follow UNESCO’s ideas and procedures for establishing property rights. My case thus illustrates the limits, and even the shortcomings, of UNESCO’s program of Masterpieces and the copyrights/droits d’auteur that it implies. Based on a representative case study of an epic text recorded in 2007 in Mali, this article calls for a methodological discussion: what does one do when terms of ownership are intrinsically impossible to conceptualize in a legal framework? First of all, I will describe the social tension created by my recording. Subsequently, I will describe the conceptual framework that I developed to deal with this tension in a culturally appropriate way. In this article I suggest that determining ownership in terms of copyrights easily runs the risk of imposing a Western political standard. As an alternative, I have explored a...

A Randomized Trial of Doxycycline forMansonella perstansInfection

Mansonella perstans infection is common in areas of Africa where Wuchereria bancrofti, a causative agent of lymphatic filariasis, is endemic. M. perstans is refractory to standard antifilarial therapies. The recent discovery of bacterial endosymbionts (e.g., wolbachia) in most filarial species, including M. perstans, provides new therapeutic options for reducing microfilaremia. In an open-label, randomized trial, we recruited subjects with M. perstans microfilaremia, with or without concomitant W. bancrofti infection, from four villages in Mali and randomly assigned them to receive doxycycline, at a dose of 200 mg daily for 6 weeks (106 subjects), or no treatment (110). At 6 months, subjects who were coinfected with W. bancrofti underwent a second random assignment, to treatment with a single dose of albendazole (400 mg) and ivermectin (150 microg per kilogram of body weight) or no treatment. Subjects were monitored daily during the first 6-week study period for adverse events. M. perstans and W. bancrofti microfilarial levels were assessed at 6, 12, and 36 months. At 12 months, 67 of 69 subjects who had received treatment with doxycycline only (97%) had no detectable M. perstans microfilariae per 60 microl of blood, as compared with 10 of 63 subjects who had received no treatment (16%) (relative risk, 6.18; 95% confidence interval, 3.63 to 11.89; P<0.001). At 36 months, M. perstans microfilaremia remained suppressed in 48 of 64 subjects who had received treatment with doxycycline only (75%), a finding that was consistent with a macrofilaricidal effect of doxycycline. Vomiting was more frequent in the doxycycline-treated group than in the untreated group (17% vs. 4%). These results are consistent with previous findings that M. perstans harbors the intracellular endosymbiont, wolbachia, and suggest that doxycycline is an effective therapy for M. perstans infection. (ClinicalTrials.gov number, NCT00340691.)

Enhancing Effectiveness of Extension Efforts: A Case Study of Malian Shea Butter Producers

The researchers conducted an ethnographic case study in three villages in Mali (West Africa) to ascertain shea butter producers’ perceptions toward technologies that improve the efficiency of shea butter production. The study revealed that the appropriateness of time and labor saving technologies for Malian women who produce shea butter depended on: 1) the relative cost of the technologies; 2) the accessibility of information on new technologies; 3) the arduousness of the work avoided; 4) the economic status of the households and of the women’s associations; and 5) the productivity of the participants in other economic activities. The study confirmed the conclusions of previous researchers who noted that external technical assistance from developed countries played a vital catalytic role in upgrading traditional technologies. Top-down technical assistance was not the best dissemination means. Participants preferred producer-led training along with visual aids. New shea butter processing technologies were requested by participants, and an understanding of the local context for technology application as well as a strong field presence of trainers during designing and testing was important for dissemination.

Validation of food variety as an indicator of diet quality assessed with a food frequency questionnaire for Western Mali.

To assess the validity of two diet quality indexes-food variety score (FVS) and diet diversity score (DDS) in Western Mali. In two separate studies (A and B), FVS and DDS were derived from a food frequency questionnaire (FFQ) and were compared with the same scores and with measures of nutrient adequacy derived from 2-day weighed records (WR). Two rural, adjacent villages in Mali, West Africa. In study A (1996), 48 women and 27 men (15-59 y) participated. Study B (1999) included 34 women and 36 men (15-45 y). Spearman's correlation coefficients between FVS from the FFQ and WR for men were 0.5 in study A and 0.4 in study B. The corresponding correlations for DDS were 0.2 and 0.4, respectively. Correlations for women were lower, especially in study B. Classification into quartiles was also best for men. Correlations for the two diet variety scores from the FFQ with nutrient adequacy from the WR in study A were about 0.3 for men, but lower for women. In study B, these correlations were around 0.5 for men, whereas for women there was no association. FVS and DDS can be assessed by our FFQ. The two diet variety scores reflect diet quality and are simple tools that can be used for monitoring. The validation study gave better results for men than for women, which has implications for further use of these instruments.

Complexities in the genetic structure of Anopheles gambiae populations in west Africa as revealed by microsatellite DNA analysis.

Chromosomal forms of Anopheles gambiae, given the informal designations Bamako, Mopti, and Savannah, have been recognized by the presence or absence of four paracentric inversions on chromosome 2. Studies of karyotype frequencies at sites where the forms occur in sympatry have led to the suggestion that these forms represent species. We conducted a study of the genetic structure of populations of An. gambiae from two villages in Mali, west Africa. Populations at each site were composed of the Bamako and Mopti forms and the sibling species, Anopheles arabiensis. Karyotypes were determined for each individual mosquito and genotypes at 21 microsatellite loci determined. A number of the microsatellites have been physically mapped to polytene chromosomes, making it possible to select loci based on their position relative to the inversions used to define forms. We found that the chromosomal forms differ at all loci on chromosome 2, but there were few differences for loci on other chromosomes. Geographic variation was small. Gene flow appears to vary among different regions within the genome, being lowest on chromosome 2, probably due to hitchhiking with the inversions. We conclude that the majority of observed genetic divergence between chromosomal forms can be explained by forces that need not involve reproductive isolation, although reproductive isolation is not ruled out. We found low levels of gene flow between the sibling species Anopheles gambiae and Anopheles arabiensis, similar to estimates based on observed frequencies of hybrid karyotypes in natural populations.

Molecular identification of sympatric chromosomal forms of Anopheles gambiae and further evidence of their reproductive isolation.

Three chromosomal forms of Anopheles gambiae s.s., designated as Bamako, Mopti and Savanna, were studied for diagnostic PCR assays based on the analysis of the X-linked ribosomal DNA (rDNA). The study was performed on a 1.3 kb fragment containing part of the 28S coding region and part of the intergenic spacer region. The amplified material was cut with fourteen restriction enzymes to detect Restriction Fragment Length Polymorphisms (RFLPs). The enzymes Tru9I and HhaI produced patterns of DNA bands which differentiated Mopti from Savanna and Bamako; moreover, a distinct 'hybrid' pattern was recognized in the F1 female progeny from the cross of Mopti with either one of the other two chromosomal forms. The diagnostic significance of the PCR-RFLP assay was verified on 203 karyotyped females from field samples collected in two villages in Mali and one village in Burkina Faso. Agreement was observed between the chromosomal and the molecular identifications. No 'hybrid' molecular patterns were detected even among carriers of rare heterokaryotypes hypothetically produced by crosses between Mopti and Savanna. The results confirm previous observations indicating barriers to gene flow within An. gambiae s.s. and supporting the specific status of the taxonomic units proposed on cytogenetic ground.

Ultrasonography of ureteric abnormalities induced by Schistosoma haematobium infection before and after praziquantel treatment.

To describe the ultrasonographic appearance of ureteric lesions due to Schistosoma haematobium infection and to report prevalence rates of such lesions in endemic communities in Mali before and after praziquantel treatment. The total population (n = 961) of three S. haematobium-endemic villages in Mali was examined ultrasonographically to detect urinary tract morbidity. Urine was tested for S. haematobium ova and for haematuria. An identical follow-up study was performed 1 year after mass treatment with praziquantel. Prevalence rates of S. haematobium infection in the three villages were 53%, 72% and 73%. Ultrasonography revealed dilatation of the ureters in 143 subjects (15%). In 20 of these, mainly children and adolescents, thickening and irregularities of the ureteric wall with distal ureter obstruction were directly visualized on ultrasonography. Within the bladder of two others, an intraluminal structure of ureterocele-like appearance was seen. None of these alterations has been previously described as an ultrasonographic feature of schistosomal uropathy. Active S. haematobium infection and microhaematuria were found in 21 and 20 of these 22 subjects respectively. Significant renal obstruction was present in 10 of them. One year after treatment, an entirely normal urinary tract was found in 85% of 104 re-examined individuals who had had ureter dilatation. Ureteric wall thickening or ureterocele-like lesions had disappeared in 95% of subjects. The role of ultrasound in the evaluation of schistosomal uropathy is confirmed. Ureteric wall abnormalities causing strictures and ureterocele-like lesions of the ostium must be regarded as further ultrasonographic features of this condition. The study documents the excellent reversibility of ureteric abnormalities after antischistosomal treatment in children and adolescents, thus emphasizing the need for early and efficient medical treatment of the infection.