Abstract The purpose of this study was to investigate the vibrational properties and corresponding anisotropicity in wood during different states of moisture sorption. Samples of maple (Acer spp.) and red oak (Quercus rubra Michx.f.) were moisture conditioned by the adsorption process from an ovendried state and by the desorption process from a water-saturated state. The dynamic modulus of elasticity (DMOE) and logarithmic decrement (δ) were examined as a function of grain orientation during moisture change processes and under constant moisture contents (MC). It was observed that regardless of species and grain direction, the DMOE and δ were lower and higher, respectively, during the moisture change process compared with those measured without a change in MC. The increase in δ value during adsorption was greater than that during desorption. These results suggest that wood in an unstable state shows lower elasticity and strength and higher damping properties than wood in an equilibrium state. Furthermore, results of this study demonstrate that a greater adsorption rate leads to greater destabilization during an adsorption process. The anisotropy in vibrational properties was found to vary between two species.