Aiming at the problem that the gun barrel vibration affects the firing accuracy during tank moving, based on the road simulation test, under the excitation of the different road levels (C, D and E), the acceleration vibration response curves of the barrel under different road levels and different vehicle speeds were obtained by arranging acceleration sensors on the barrel surface. Based on the power spectrum analysis method, the frequency response characteristics of the barrel vibration were analyzed, and the main frequency of barrel vibration during moving is obtained. It has been shown that, the main frequency of the barrel response is mainly distributed in three frequency ranges: 1~3Hz, 14~15Hz and 24~26Hz. The main frequency of the barrel vibration is different with different roads. The main frequency of the barrel response is mainly in the frequency range of 1~3Hz under C level road, and in the frequency range of 1~3Hz and 24~26Hz under D lever road. And under the E level road, the main frequency of barrel response is within the three frequency ranges of 1~3Hz, 14~15Hz and 24~26Hz. And the main frequency is 25Hz as the measuring point is far away from the muzzle. The results will provide support for obtaining the vibration characteristics of barrel as tank moving and explaining the vibration response law of the barrel.