Occupant comfort is a key consideration in automobile dynamics, with vibrations potentially causing long-term physical discomfort, especially for drivers. This study investigates the impact of adding TiO2 and CuO nanoparticles to fuel on engine-induced vibrations. Experiments were conducted at various nanoparticle concentrations (0, 50, 100, and 150 ppm) and engine speeds (1000, 2000, and 3000 rpm). Key performance metrics, including kinematic viscosity, density, heating value, thermal conductivity, and brake power (BP), were analyzed. The results indicated that increasing nanoparticle concentration led to a rise in BP. The highest reduction in root mean square (RMS) vibration accelerations occurred at 3000 rpm and 150 ppm, with vibration reductions of 30.33% for CuO and 28.61% for TiO2. Additionally, 8–10% of engine vibrations were transmitted to the steering wheel. The use of 150 ppm CuO nanoparticles resulted in reduced vibration transmission to the steering wheel at all tested speeds. These findings suggest that nanoparticle-enhanced fuels can significantly reduce engine vibrations, potentially improving driver comfort and reducing wear on vehicle components.