Dynamic stabilization of large machines is necessary procedures before the commencement of operations railway lines that are newly built. However, limited or no studies have been carried out to reveal the operational mechanism from the vibration transfer characteristics of ballasted track subjected to excitation by stabilizing machines. The focus of this study is to solve this shortcoming. First, a typical new railway line was selected to test the dynamic response of the ballasted track during stabilizing operation, and then a stabilizing machine-ballasted track model was established with the help of the DEM-MBD coupling method. And the biaxial vibration accelerometers were inserted at various locations in the ballast bed to monitor the evolution of the vibration level on real-time basis. The influence of the stabilizing operation process on the vibration transmission and attenuation characteristics of sleeper and ballast bed was explored using wavelet analysis and Fast Fourier Transform method. The vibration absorption capacity and energy dissipation characteristics of the ballasted track under various stabilizing frequencies were analyzed. At the same time, the contact state and movement posture of ballast particles during the stabilizing operation were explored. Results showed that the vertical vibration attenuation rate of the slope toe of the ballast bed after the first stabilizing machine pass is 92.32%, while the vertical vibration attenuation rate of the second stabilizing machine is reduced by 3.14%. The second stabilizing machine can cause a large lateral movement of the ballast at the bottom of the sleeper. Under the excitation of 25[Formula: see text]Hz stabilizing frequency, it was observed that, the vibration absorption capacity of the ballast bed at the bottom of the sleeper is high, the vibration level at the ballast bed slope is low, and the stability is better. This is the optimal frequency for the stable operation of the new railway. This study can provide theoretical support for the establishment of the internal correlation mechanism between the vibration characteristics of stabilizing machines and the mechanical state of the ballast bed.