Vertical Sediment Research Articles

Metagenomic analysis of sedimentary archives reveals ‘historical’ antibiotic resistance genes diversity increased over recent decades in the environment

Abstract Antibiotic Resistance Genes (ARGs) are widespread in freshwater environments and represent a concealed threat to public health and aquatic eco-system safety. To date, only a limited number of studies have investigated the historical distribution of ARGs and their hosts through the analysis of freshwater sedimentary archives. This research gap constrains our comprehensive of the mechanisms underlying natural bacterial resistance formation during pre-antibiotic era (prior to the 1940s) and the development of human-induced bacterial resistance in post-antibiotic era (since the 1940s). In this study, we examined the vertical distribution patterns of ARGs and their associated hosts within a sedimentary core from a eutrophic lake, employing shotgun sequencing methodology. The findings revealed a marked increase in ARG diversity during post-antibiotic era, and the predominant ARG types identified included those conferring resistance to multidrug, bacitracin, macrolide–lincosamide–streptogramin, beta-lactam, tetracycline, fluoroquinolone, glycopeptide and aminoglycoside, collectively accounting for 78.3%–85.6% of total ARG abundance. A total of 127 ARG subtypes were identified in samples, and 48 ARG subtypes shared across vertical sediment resistome profile with two of them, bacA and bcrA, occurring only in post-antibiotic era. Further, 137 metagenome-assembled genomes (83 species belonging to 12 phyla) were identified as ARG hosts, mainly belonging to the phyla Proteobacteria, Nitrospirota, Chloroflexota, Bacteroidota, Actinobacteriota, Cyanobacteria, and Firmicutes. Significant correlation was found between the diversity of ARG and the concentrations of organic matter and heavy metals, suggesting a common source of contamination. Aside the fact that human-induced eutrophication is a forcing factor acting in parallel to increase ARGs releases in water systems, both being indicators of increased urbanization in the catchment, eutrophication may significantly increase bacterial activity, thereby facilitating the spread of antibiotic-resistant bacteria in environment. This study reveals the marked increased in ARG diversity with the onset of antibiotic use by human societies with potential impact of aquatic ecosystem.

Paleostorm redeposition and post-glacial coastal chronology in the eastern Baltic Sea, Latvia

The 494 km long Latvian coast consists of sandy sediments, which serve as an excellent proxy for past coastal events and environment. Our study explores these understudied sediments along the western coast of the Gulf of Riga and combines two sand quartz-wise methods: optically stimulated luminescence (OSL) dating and grain microtextures. We provide a new chronology and microsedimentary results to trace post-glacial storm events and discuss correlations within the Baltic Sea region and elsewhere in Europe. OSL ages reveal a wide time span between 10.38 ± 0.61 ka and 1.04 ± 0.05 ka BP, but their inconsistency in the profiles along with the onshore position of landforms and gravelly horizons argues for sediment relocation due to storm action. A first paleostorm phase is dated to between 7.6 ± 1.2 ka and 4.63 ± 0.27 ka, corresponding with a transgression of the Littorina Sea and the Holocene Thermal Maximum, and with a roughly estimated vertical sediment redeposition above 5 m similar with the recent storms in the region. Sedimentary record from microstudy supports intense storm activity, especially in sediments redeposited after 6.07 ± 0.51 ka, and it is seen through an increased number of cracked quartz grains, shiny grains and fresh surfaces combined with a limited number of V-shaped marks. Weaker storm action, recorded by a lower share of cracked grains in the sediments, occurred between 7.6 ± 1.2 ka and 6.07 ± 0.51 ka.A second storm phase occurred at 1.44 ± 0.21 ka, corresponding with the post-Littorina, again with a performance of a weaker storm. Apart from the palaeostorm records, the beach ridges developed between 5.16 ± 0.33 ka and 4.47 ± 0.31 ka along with drier conditions when aeolian deposition took place twice: at 4.63 ± 0.27 ka and 1.55 ± 0.10 ka.

Long-Term Effects of 90Sr and 239+240Pu Contaminated on the Seafloor Sediment on the Transfer through Marine Food Web: Sensitivity to the Physical Property- and Sediment-Related Parameters

This study, as a follow-up to the <sup>137</sup>Cs study of Kim et al. (2023), performs a set of sensitivity experiments on ocean physical properties and sediment-related parameters based on initial bottom contamination of <sup>90</sup>Sr and <sup>239+240</sup>Pu in the upper sediment layer. Both studies utilizes the Extended BURN model proposed by Bezhenar et al. (2016). The initial contamination levels were set to 3 and 0.4 Bq/kg, respectively, based on observations off the coast of Fukushima. The sensitivity experiments were divided into EXP-WOHAD, which examined the effects of the vertical diffusion coefficient and vertical sediment flux, and EXP-WHAD, which investigated the effects of ocean currents. The results of the EXP-WOHAD experiments showed that both radionuclides are more sensitive to sediment fluxes than to vertical diffusion coefficients. For both <sup>90</sup>Sr and <sup>239+240</sup>Pu, transport was dominated by transport to the intermediate sediment layer rather than to seawater. Specifically, for <sup>239+240</sup>Pu, transport to seawater was insignificant due to its strong sorption properties in sediments. In terms of bioaccumulation, <sup>90</sup>Sr accumulated most in fish and invertebrates, while <sup>239+240</sup>Pu accumulated most in invertebrates, followed by zooplankton. Notably, <sup>239+240</sup>Pu showed high bioaccumulation despite its low initial sediment contamination. The changes in radioactivity concentrations in the EXP-WHAD experiments were similar to those in the EXP-WOHAD experiments, and the bioaccumulation trends in biota were also comparable. This study confirms that the accumulation of radionuclides in seawater, intermediate sediments, and biota is sensitive to the marine physical environment and varies by nuclide. When the sediment flux increased by a factor of 100, the remaining radioactivity levels of <sup>90</sup>Sr and <sup>239+240</sup>Pu in biota after 100 years showed a significant reduction, decreasing by more than 900 and 30,000 times, respectively; it highlighting the importance of parameterization according to the physical environment of the area of interest.

Vertical variation of antibiotic resistance genes and their interaction with environmental nutrients in sediments of Taihu lake

Antibiotic resistance is a growing environmental issue. As a sink for antibiotic resistance genes (ARGs), lake surface sediments are well known for the spread of ARGs. However, the distribution pattern of ARGs and their relationship with environmental factors in vertical sediment layers are unclear. In this study, we investigated the resistome distribution in sediment cores from Taihu Lake using metagenomic analysis. The results showed that the abundance of total ARGs increased by 153% as the sediment depth rose from 0 to 50 cm, and the ARG Shannon index significantly increased. Among all the ARG types, efflux pump genes (e.g., mexT and mexW) were dominant, especially in 40–50 cm sediment. The variation in ARG with depth described above was related to the changes in bacterial adaptation to environmental gradients. Specifically, sulfate and nitrate concentrations decreased with depth, and random forest analysis showed that they were the main factors affecting the changes in ARG abundance. Environmental factors were also found to indirectly impact the distribution of ARGs by affecting the bacterial community. Potential sulfate-reducing gene/nitrate-reducing gene-ARG co-hosts were annotated through metagenomic assembly. The dominant co-hosts, Curvibacter, and Comamonas, which were enriched in deeper sediments, may have contributed to the enrichment of ARGs in deep sediments. Overall, our findings demonstrated that bacterial-mediated sulfate and nitrate reduction was closely related to sediment resistance, which provided new insights into the control of antibiotic resistance.

Characteristics of the Aeolian Sediments Transported Above a Gobi Surface

AbstractGobi’s (gravel deserts) are one of the largest dust sources in northern China. Previous studies indicated that sand transport processes above the surface differed between Gobi and sand surfaces. However, the sand transport rate and related dust emission processes above Gobi (gravel) surfaces are still poorly understood. In this field study, we quantified this transport to provide important support for parameterizing Aeolian sediment transport models and clarifying the relationship between dust emission and transport. Threshold wind velocity can reach 0.38 ± 0.04 (mean ± SD) m s−1 above Gobi surfaces. Compared to the most commonly used sand‐transport models, we found that the Lettau and Lettau sediment transport model can be used to calculate horizontal sediment transport above a Gobi surface. The relationship between the vertical sediment transport (Fs) and shear velocity could be expressed using a power function. Although the horizontal sand transport and vertical flux (Q and Fs, respectively) above Gobi surfaces can be expressed similarly to previous results (i.e., using similar equation forms), the equation coefficients were much larger for the Gobi surface than for a shifting sand surface; that is, sediment transport was higher above the Gobi surface. This difference resulted from the larger sand transport rate and saltation height above the Gobi surface, and the larger transport and higher saltation height were related to the larger sand transport height and higher content of coarse sand transported above the Gobi surface.

Cyanobacteria decay alters CH4 and CO2 produced hotspots along vertical sediment profiles in eutrophic lakes

Cyanobacteria-derived organic carbon has been reported to intensify greenhouse gas emissions from lacustrine sediments. However, the specific processes of CH4 and CO2 production and release from sediments into the atmosphere remain unclear, especially in eutrophic lakes. To investigate the influence of severe cyanobacteria accumulation on the production and migration of sedimentary CH4 and CO2, this study examined the different trophic level lakes along the middle and lower reaches of the Yangtze River. The results demonstrated that eutrophication amplified CH4 and CO2 emissions, notably in Lake Taihu, where fluxes peaked at 929.9 and 7222.5 μmol/m2·h, mirroring dissolved gas levels in overlying waters. Increased sedimentary organic carbon raised dissolved CH4 and CO2 concentrations in pore-water, with isotopic tracking showing cyanobacteria-derived carbon specifically elevated CH4 and CO2 in surface sediment pore-water more than in deeper layers. Cyanobacteria-derived carbon deposition on surface sediment boosted organic carbon and moisture levels, fostering an anaerobic microenvironment conducive to enhanced biogenic CH4 and CO2 production in surface sediments. In the microcosm systems with the most severe cyanobacteria accumulation, average CH4 and CO2 concentrations in surface sediments reached 6.9 and 2.3 mol/L, respectively, surpassing the 4.7 and 1.4 mol/L observed in bottom sediments, indicating upward migration of CH4 and CO2 hotspots from deeper to surface layers. These findings enhance our understanding of the mechanisms underlying lake sediment carbon emissions induced by eutrophication and provide a more accurate assessment of lake carbon emissions.

Quantitative characterization of microstructure and research on spatial variation characteristics of loess of different strata in Luochuan, Shaanxi, China.

The complete sequence of loess strata in Luochuan has become a typical section in loess strata, and is the main focus of research for many scholars studying loess. We were based on the theory of aeolian loess and established a set of quantitative index parameters for loess microstructure through our previous research, such as equivalent diameter, sphericity, morphology ratio, orientation angle Phi, orientation angle Theta, pore Eq-Radius, throat Eq-Radius and throat channelLength. Through the quantitative characterization of various index parameters of the Luochuan loess, we found that the probability density of each index parameter meets a specific distribution well, and in terms of spatial dimension, it shows that as the depth of the strata increases, the average particle size and the mode of pore Eq-Radius, throat Eq-Radius and throat channelLength generally increase, while the mode of particle morphology ratio generally decreases. In addition, loess particles in deeper strata are less prone to vertical sedimentation and tend to deposit gently or horizontally. Most particles in different strata are distributed in a northwest or southwest direction. During the formation period of strata, the main cause for spatial differences is the material carrying force. We conducted a statistical analysis on the correlation between the macroscopic physical properties of loess and its microstructure index parameters. Specifically, we found a positive correlation between loess density and the average particle size and the mode of particle equivalent diameter, Additionally, we found a negative correlation between loess liquid limit and plastic limit, and the mode of particle morphology ratio. Furthermore, there was a negative correlation between permeability coefficient and the mode of pore Eq-Radius, throat Eq-Radius, and throat channelLength.

Real-time and high-sensitive colorimetric sensor based on photonic crystal for amniotic fluid identification

In this study, we proposed a pH-responsive photonic crystal hydrogel sensor (PCHs) to conveniently and accurately recognize the amniotic fluid from urine of parturient women, which was constituted by three-dimensional polymethyl acrylamide (PMMA) array and optimized hydrogel. PMMA array was self-assembled by 180 nm monodispersed nanoparticles with vertical sedimentation method. The functional hydrogel containing N-acryloyl phenylalanine (APA), acrylamide (AM) and N, N'-methylene bis(acrylamide) (BIS) as co-monomer and crosslinking agent respectively, was photo-polymerized by the precursor under 365 nm UV light. This hydrogel significantly shrank or swelled corresponding to the acidity and alkalinity of the aqueous solution, which resulted in both the reflection peak position (λmax) of the prepared sensor and its structural color changing with the pH value. The λmax of PCHs redshifted from 514 nm to 606 nm in 60 s while the pH value of testing solution increased from 4.5 to 8.0, which exactly covered the pH value of amniotic fluid and urine. Correspondingly, its structure color gradually changed from pale-green to red ensuring their naked-eye determination. Furthermore, an extremely high sensitivity as 26.3 nm/pH of Δλmax was obtained which was linearly related to pH value. The prepared sensor possessed steady accuracy and extraordinary sensitivity compared with the pH test strip which was generally used in amniorrhexis diagnosis nowadays besides the advantages of the low cost, simple operation, rapid response and visual detection mode. The reliability of this PCHs was further confirmed by the real sample detection which relative standard deviation was below 5.0 %, suggesting its potential clinical applications.

Investigation of the Influence of Operating Parameters on the Settling Performance of a Vertical Sedimentation Tank Through Computational Fluid Dynamics Simulations

Investigation of the Influence of Operating Parameters on the Settling Performance of a Vertical Sedimentation Tank Through Computational Fluid Dynamics Simulations

Quantifying the scale of erosion along major coastal aquifers of Pakistan using geospatial and machine learning approaches.

Insufficient freshwater recharge and climate change resulted in seawater intrusion in most of the coastal aquifers in Pakistan. Coastal aquifers represent diverse landcover types with varying spectral properties, making it challenging to extract information about their state hence, such investigation requires a combination of geospatial tools. This study aims to monitor erosion along the major coastal aquifers of Pakistan and propose an approach that combines data fusion into the machine and deep learning image segmentation architectures for the erosion and accretion assessment in seascapes. The analysis demonstrated the image segmentation U-Net with EfficientNet backbone achieved the highest F1 score of 0.93, while ResNet101 achieved the lowest F1 score of 0.77. Resultant erosion maps indicated that Sandspit experiencing erosion at 3.14 km2 area. Indus delta is showing erosion, approximately 143 km2 of land over the past 30years. Sonmiani has undergone substantial erosion with 52.2 km2 land. Miani Hor has experienced erosion up to 298 km2, Bhuri creek has eroded over 4.11 km2, east Phitii creek over 3.30 km2, and Waddi creek over 3.082 km2 land. Tummi creek demonstrates erosion, at 7.12 km2 of land, and East Khalri creek near Keti Bandar has undergone a measured loss of 5.2 km2 landlinked with quantified reduction in the vertical sediment flow from 50 (billion cubic meters) to 10 BCM. Our analysis suggests that intense erosions are primarily a result of reduced sediment flow and climate change. Addressing this issue needs to be prioritized coastal management and climate change mitigation framework in Pakistan to safeguard communities. Leveraging emerging solutions, such as loss and damage financing and the integration of nature-based solutions (NbS), should be prioritized for the revival of the coastal aquifers.

Self-hydrolyzing Submerged Flow Technology for Acid Coal Mine Drainage Treatment: Effective Removal of Iron Ions

In this study, mine wastewater from Leiyang, Hunan Province was taken as the research object to investigate the effect of using the method of self-hydrolytic submerged sedimentation to treat acid coal mine drainage. It was found in this study that the removal rate of sulfate ions and iron ions from the acidic coal mine drainage stock solution at pH=2.7 was very limited by self-hydrolytic sedimentation experiments relying only on pure vertical sedimentation flow treatment, but the hydrolytic treatment could greatly improve the solution environment and promote the formation of secondary minerals of sulfate ions and iron ions in the acidic coal mine drainage, which greatly improved the removal rate of iron ions. Both suspended and precipitated materials produced in the self-hydrolysis sedimentation experiments were hydroxylated iron sulfate secondary minerals- Schwertmannite [Fe8O8(OH)8-2X(SO4)X (1 ≤ x ≤ 175)], the formation of which is inextricably linked to the low pH environment and high concentration of sulfate and iron ions in coal mine wastewater. Self-hydrophoretic submerged flow can remove iron ions from acidic coal mine drainage at low cost and high efficiency, and the total iron removal rate of this system can reach 90.93% after 24 h of treatment, which has a favorable comprehensive utilization value.

Solving the one dimensional vertical suspended sediment mixing equation with arbitrary eddy diffusivity profiles using temporal normalized physics-informed neural networks

Analytical solutions are practical tools in ocean engineering, but their derivation is often constrained by the complexities of the real world. This underscores the necessity for alternative approaches. In this study, the potential of Physics-Informed Neural Networks (PINN) for solving the one-dimensional vertical suspended sediment mixing (settling-diffusion) equation which involves simplified and arbitrary vertical Ds profiles is explored. A new approach of temporal Normalized Physics-Informed Neural Networks (T-NPINN), which normalizes the time component is proposed, and it achieves a remarkable accuracy (Mean Square Error of 10−5 and Relative Error Loss of 10−4). T-NPINN also proves its ability to handle the challenges posed by long-duration spatiotemporal models, which is a formidable task for conventional PINN methods. In addition, the T-NPINN is free of the limitations of numerical methods, e.g., the susceptibility to inaccuracies stemming from the discretization and approximations intrinsic to their algorithms, particularly evident within intricate and dynamic oceanic environments. The demonstrated accuracy and versatility of T-NPINN make it a compelling complement to numerical techniques, effectively bridging the gap between analytical and numerical approaches and enriching the toolkit available for oceanic research and engineering.

Azoic sediments and benthic foraminifera: Environmental quality in a subtropical coastal lagoon in the gulf of California

Marine transitional environments play an important role in human sustainability. Around these ecosystems, coastal lagoons are subject to high anthropogenic pressure from population growth. The increased demand for goods and services is associated with the elevated discharge of untreated and treated wastewater into lagoon systems. The absence of benthic organisms in lagoon environments has been linked to extreme natural conditions and severe anthropogenic impact at both spatial and temporal scales. However, the mechanisms that lead to the presence of azoic sediments in lagoon environments have yet to be studied. This study aimed to determine the vertical variability of textural groups, geochemistry, and benthic foraminiferal fauna to understand how natural and anthropogenic components generate a vertical sediment sequence with low or absent benthic foraminifera in a subtropical coastal lagoon in the southwestern end of the Gulf of California. A 41 cm-long sediment core was collected from La Paz Lagoon at a 1-m depth. The core was sectioned every centimeter, and sediment subsamples were dried and homogenized for grain size, calcium carbonate, elemental and isotopic carbon and nitrogen analyses, and benthic foraminifera quantification. Muds with fine sands towards the core's base characterized the sedimentary sequence. Organic carbon and total nitrogen increased from the base (1.4% and 0.06%, respectively) to the core-top (CT, 3.0% and 0.14%, respectively), significant from the 27 cm interval. Calcium carbonate content was very low (<0.8%). The relationship of δ13C vs. C:N ratio indicated that sedimentary organic carbon was derived from the marine and sewage source mixture. The δ15N of organic matter increased by 3.7‰, starting from the 27 cm interval towards the CT. The nitrogen sewage input source was relatively more significant than nitrogen fixation. The few individuals (<18 ind. in 10 g) and genera (Ammonia and Elphidium), as well as the absence of foraminifera in 19 of 41 intervals in the core, indicated that environmental conditions were unfavorable, even for colonization of environmentally stress-tolerant genera. The frequency of azoic sediments was higher from the 25 cm interval to the CT vs. from the base to the 25 cm interval. Moreover, the AEI revealed severe to moderate hypoxia in the study area. The limited presence of benthic foraminifera and calcium carbonate preservation corroborated that the quality of the lagoon's environment has deteriorated along with population growth, which requires strategic programs to sustain this transitional ecosystem.

Sedimentation of Crassostrea gigas and Perna perna biodeposits in the South Atlantic

This study aimed to determine the vertical sedimentation velocity of fecal and pseudofecal matter from the bivalve molluscs Crassostrea gigas and Perna perna in the Santa Catarina Island bays, focusing on two diferente locations: Caieira da Barra do Sul (CBS) and Sambaqui (SBQ). The initial phase involved collecting biodeposits using a system with twelve chambers, directing a controlled seawater flow at a rate of 500 mL.min−1. The organisms were individually housed in these chambers, and biodeposits were collected over a three-hour period. Fourteen campaigns were conducted to collect biodeposits from the two species under study. Three types of samples were produced after each campaign: feces, pseudofeces, and a combination of both (F, PF, and F/PF, respectively). The subsequent phase involved evaluating sedimentation velocity in a controlled environment using a two-meter-long, 150 mm-diameter acrylic column filled with filtered seawater. Sedimentation tests were conducted using samples from the initial phase. The sample was inserted at the top of the column, and turbidity was measured over a specified period (up to 25 min). The sedimentation velocities for F were as follows: for C. gigas, 1.56 ± 0.19 cm.s−1 and 2.08 ± 0.83 cm.s−1 in CBS and SBQ, respectively, and for P. perna, the corresponding values were 2.36 ± 0.70 cm.s−1 and 3.33 ± 0.00 cm.s−1, respectively. For F/PF samples, the corresponding sedimentation velocities were: 2.41 ± 0.85 cm.s−1 and 1.15 ± 0.61 cm.s−1 for C. gigas and 2.36 ± 0.70 cm.s−1 and 2.96 ± 0.64 cm.s−1 for P. perna, in CBS and SBQ, respectively. The sedimentation rates of samples composed solely of pseudofeces could not be determined, as the particles in these samples did not settle within the 25-minute analysis period. The velocities determined were closely related to the characteristics of particles found in the marine water habitat of the organisms. Their filtration-based feeding mechanism significantly influences the vertical sedimentation velocity and biodeposit formation. The methodology used to determine sedimentation velocity proved to be effective and quick, offering a more cost-effective approach than other available methodologies.

Development of a Marine Radioactivity Expert System based on the Extended Marine Food Web: Experiments with Initial Bottom Contamination

An extended version of an expert system was developed as an effort to provide technical support to decision makers for the initial prompt assessment of environmental impacts of marine radioactivity, considering both pelagic and benthic marine food webs. The preliminary version of this system was firstly developed to determine the transfer of radionuclides through a pelagic marine food web (Kim et al, 2020). This system's approach was similar to the Cornell Mixing Zone Expert System (CORMIX) developed by Jirka (1995), as it used idealized settings for the physical/hydrodynamic coastal conditions. Existing or extended systems were executed by taking into account different kinds of radioactivity sources. The initial contamination of bottom sediment is considered in the present study, while the direct discharge to the coastal ocean from the nuclear power plant was considered in the early study. Assuming initial contamination, sensitivity experiments of temporal &lt;sup&gt;137&lt;/sup&gt;Cs variations without/with horizontal advection and diffusion (EXP-WOHAD and EXP-WHAD, respectively) were conducted. In EXP-WOHAD, a total of six test cases were constructed, considering a range of vertical diffusion coefficients and sediment fluxes at the water-sea bed interface. The EXP-WHAD experiments were classified into two categories with six cases each, considering the horizontal diffusion and advection processes. The EXP-WOHAD results show that the vertical diffusion processes in the bottom sediment result in the exponential decay of the &lt;sup&gt;137&lt;/sup&gt;Cs concentrations in the top sediment layer through upward and downward diffusive fluxes, producing pulse-like time variations in &lt;sup&gt;137&lt;/sup&gt;Cs in the water column and the middle sediment layer. These sediment fluxes affect the peak height and its occurrence time in the water column and the middle sediment layer. From EXP-WHAD, it has been confirmed that part of &lt;sup&gt;137&lt;/sup&gt;Cs diffused into the water column experiences horizontal dispersion and thereafter deposition to the bottom sediment. It is noted that due to the limited diffusion to the water column, the &lt;sup&gt;137&lt;/sup&gt;Cs concentrations in benthic organisms are larger than those in pelagic organisms.

Hybrid engineering incorporating salt marsh terraces into sea wall repair maintains their defence function and creates new habitats

Sea walls are widely-used engineered structures designed to protect low lying land from flooding. Rising sea levels and coastal erosion threatens sea wall integrity and requires maintenance and repair. As an alternative to conventional repairs, a hybrid engineering design incorporating sediment-filled terraces to allow the development of salt marsh has been trialled to protect sea walls in the Colne-Blackwater Estuary complex, Essex. U.K. Over a 10 year period, three different trajectories of development were measured. Salt marsh halophytes colonised 7 out of 15 terraces, with % plant cover ranging between 5% and 170%. Halophyte species richness on terraces ranged from 1 to 8 species (average = 1.7 per 4 m2), compared to existing salt marsh adjacent to the repairs (max. species richness 9, average 6.85), with positive significant relationships between % halophyte cover and halophyte richness and between % halophyte cover and sediment water content (19–51% on terraces). Organic carbon content was significantly lower on the terraces compared to existing salt marsh (7–19 % AFDW on terraces, 17–24% in marsh sediments). A sediment shear strength of 30 kPa was optimal for % plant cover on terraces. The height of the terraces relative to tidal inundation was a key determinant of successful halophyte colonisation. A second trajectory (3 of 15 terraces) resulted in development of macro- and microalgal mats, up to 100 % cover. Both these trajectories resulted in up to 25 cm of vertical sediment accretion. Shell banks formed on 2 terraces. Seven terraces were subject to erosion, associated with desiccation and absence, or loss, of halophyte cover. Eroding terraces showed both surface lowering (20–30 cm) and massive sediment failure (blocks >50 cm deep falling away). After a decade, protection of the sea wall was still provided by 14 of the 15 terraces, with only one having to be rebuilt. Utilising a nature-based approach incorporating sea wall terraces resulted in the formation of three types of linear habitat (salt marsh, microbial mats, shell banks) providing some ecosystem services that would not have existed if a conventional hard-engineered repair had been used.

Exploratory modelling of the impacts of sea-level rise on the Sundarbans mangrove forest, West Bengal, India

In this paper we conduct exploratory simulations of the possible evolution of the Indian Sundarbans mangroves to 2100 under a range of future sea-level rise (SLR) scenarios, considering the effects of both inundation and shoreline erosion. The Sea Level Affecting Marshes Model (SLAMM) is used to simulate habitat transitions due to inundation and these outputs are combined with an empirical model of SLR-driven shoreline erosion. A set of plausible climate-induced SLR scenarios are considered, together with delta subsidence and constrained vertical sediment accretion. Significant mangrove decline is found in all cases: the greater the rise in sea level the greater the losses. By the end of the century, the Indian Sundarbans mangroves could lose between 42 % and 80 % of their current area if current management is continued. Managed realignment could offset these losses but at the expense of productive land and the migration of the human population.

Vertical variation in prokaryotic community composition and co-occurrence patterns in sediments of the Three Gorges Reservoir, China

Archaea and bacteria are distributed throughout the sediment; however, our understanding of their biodiversity patterns, community composition, and interactions is primarily limited to the surface horizons (0–20 cm). In this research, sediment samples were collected from three vertical sediment profiles (depths of 0–295 cm) in the Three Gorges Reservoir (TGR), one of the largest reservoirs in the world. Through 16S rRNA sequencing, it was shown that sediment microbial diversity did not significantly vary across the sediment. Nevertheless, a decline in the similarity of archaeal and bacterial communities over distance along sediment vertical profiles was noted. Nonmetric multidimensional scaling (NMDS) analysis revealed that archaeal and bacterial communities could be clearly separated into two groups, located in the upper sediments (0–135 cm) and deep sediments (155–295 cm). Meanwhile, at the fine-scale of the vertical section, noteworthy variations were observed in the relative abundance of prominent archaea (e.g., Euryarchaeota) and bacteria (e.g., Proteobacteria). The linear discriminant analysis effect size (LEfSe) demonstrated that twenty-four bacterial and twenty-six archaeal biomarker microbes exist in the upper and deep sediment layers. Each layer exhibited distinctive microbial divisions, suggesting that microbes with diverse biological functions are capable of thriving and propagating along the sediment profile. Co-occurrence network analysis further indicated that the microbial network in the upper sediments was more complex than that in the deep sediments. Additionally, the newly discovered anaerobic methanotrophic archaeon Candidatus Methanoperedens was identified as the most abundant keystone archaeal taxon in both sediment layers, highlighting the significance of methane oxidation in material cycling within the TGR ecosystem. In summary, our study examined the biodiversity and coexistence patterns of benthic microbial communities throughout the vertical sediment profile, providing detailed insights into the vertical geography of archaeal and bacterial communities in typical deep-water reservoir ecosystems.

Reed restoration decreased nutrients in wetlands with dredged sediments: Microbial community assembly and function in rhizosphere

Using dredged sediments as substrate for aquatic plants is a low-cost and ecological friendly way for in situ aquatic ecological restoration. However, the limited information available about how aquatic plant restoration affects the microbial ecology and nutrients in dredged sediments. In this study, nutrient contents, enzyme activities, and bacterial and archaeal communities in vertical sediment layers were determined in bulk and reed zones of wetlands constructed with dredged sediments in west Lake Taihu for three years. Reed restoration significantly decreased total nitrogen, total phosphorus, and organic carbon contents and increased alkaline phosphatase, urease, and sucrase activities compared to bulk area. Bacterial communities in vertical sediment layers had higher similarity in reed zone in comparison to bulk zone, and many bacterial and archaeal genera were only detected in reed rhizosphere zones. Compared with the bulk zone, the reed restoration area has a higher abundance of phylum Actinobacteriota, Hydrothermarchaeota, and class α-proteobacteria. The assembly process of the bacterial and archaeal communities was primarily shaped by dispersal limitation (67.03% and 32.97%, respectively), and stochastic processes were enhanced in the reed recovery area. Network analysis show that there were more complicated interactions among bacteria and archaea and low-abundance taxa were crucial in maintaining the microbial community stability in rhizosphere of reed zone. PICRUST2 analysis demonstrate that reed restoration promotes metabolic pathways related to C and N cycle in dredged sediments. These data highlight that using dredged sediments as substrates for aquatic plants can transform waste material into a valuable resource, enhancing the benefits to the environment.

Wide bedrock valley development and sensitivity to environmental perturbations: Insights from flume experiments in erodible bedrock

AbstractWide bedrock valleys and their genetic descendants, strath terraces, can serve as morphological records of past climate that reflect river discharge and sediment load during periods of valley widening. Understanding how changes in sediment load and water discharge create such distinct morphological features is limited by a lack of robust understanding of the specific processes of bedrock valley widening. We present results from the first set of flume experiments specifically devoted to exploring the conditions necessary to create wide bedrock valleys and how bedrock valleys develop through time. We ran six experiments in a weak bedrock substrate representing valley widening in an easily erodible bedrock, with differing amounts of water discharge, sediment load and base level fall. We evaluated valley width, valley wall height, channel mobility, lateral and vertical bed incision and sediment cover on the bed to explore the conditions necessary for the development of wide bedrock valleys and better understand the processes that affect valley widening rates. The results of the experiments show that wide bedrock valleys developed slowly and only under long periods of high sediment conditions, while vertical incision occurred much faster and was easily induced under different forcing mechanisms. We found that high sediment flux, enough to cover the channel bed, was a necessary condition for substantial valley widening. However, sediment cover on the bed was not by itself a sufficient condition to create wide bedrock valleys in our experiments; other factors were also required, particularly mobile channels within the valleys and some channel curvature to induce lateral undercutting. The results from this set of experiments suggest that the creation of wide bedrock valleys has several necessary conditions that must be met, and the development of a wide bedrock valley can be disrupted by slight changes in one of these necessary conditions.