Vertical Garden Constructed Wetland (VGCW) system has been used as a secondary treatment system to improve effluent quality of septic tanks and cesspools by enhancing nutrients, organic and solid removal according to the guidelines of effluent discharge. This study aimed to investigate the treatment performance of the VGCWs by integrating with diverse plant species and modified media used as secondary treatment for conventional or solar septic tanks. In the study, laboratory-scale VGCW utilizing soil as the media and planted with Water Pennywort were operated at hydraulic retention times (HRT) of 6, 12, and 24 h, while modified planted vertical garden constructed wetlands (mpVGCW) operated at an HRT of 36 h, was fed with effluent collected from a septic tank. The treatment performance of VGCWs treating effluent from the septic tank and operating at an HRT of 24 h was found to achieve the highest removal efficiencies for organic matter, nutrients, and solids (up to over 90%), compared to an HRT of 6 or 12 h. Moreover, the highest log reduction of E.coli of 2.8 was also observed at the 24 h HRT, in comparison to HRTs of 6 or 12 h. The Spider Ivy, Coleus, and Selaginella frosty could be planted in the mpVGCW system to enhance the overall treatment efficiencies with consistently showed high removal efficiencies for TCOD (91.37–93.39%), TKN (97.1–97.8%), and TP (87.18–88.58%). These results suggested the potential of the VGCWs as a secondary treatment system for polishing septic tank effluent and improving environmental quality.