The present study investigates the role of gravity wave induced seed perturbations in the occurrence of Equatorial Spread F (ESF) under the influence of the post sunset background conditions modulated by prevailing electrodynamics and neutral wind. Ionospheric foF2 data sets over geomagnetic equatorial station Trivandrum (8.5°N, 77°E and magnetic dip 0.68°N-corresponding to the period of study) corresponding to vernal and autumnal equinoctial periods encompassing high, low and moderate solar activity years, are used for the study. Meridional wind data is obtained either from ESA’s sun-synchronous satellite GOCE (Gravity field and steady-state Ocean Circulation Explorer) or derived using ionosonde h’F (base height of ionosphere at 2.5 MHz) data from Trivandrum (TVM- 8.5°N, 77°E and magnetic dip 0.68°N) and Sriharikota (SHAR−13.7°N, 80.2°E and magnetic dip 6.9°N-for period of study). This particular study is carried out for geomagnetically quiet days of Vernal Equinox (VE) and Autumnal Equinox (AE) seasons, which are most favoured for ESF occurrence over Indian longitudes. Considering thermospheric wind, ion-neutral collisions, and electric field effects in association with gravity wave seed, threshold curve is generated, which clearly demarcates ESF and NSF (Non spread F) days. Previous studies have addressed ESF variability in electrodynamical domain alone (wherein the layer is above a threshold level). The present study, for the first time, succeeds in demarcating ESF and NSF days by incorporating effects of electric field, neutral wind, collisional RT instability term, and gravity wave seed perturbations simultaneously irrespective of threshold height.