Microwave irradiation, as a novel, clean, and efficient rock-breaking technology, can be employed in shale gas exploitation to address the challenges of low fracture efficiency and environmental pollution. Therefore, it is essential to investigate the physico-mechanical properties of shale under microwave irradiation. Firstly, samples with different bedding angles (0°, 30°, 60°, and 90°) were subjected to microwave irradiation at a power of 1 kW and frequency of 2.45 GHz for durations of 0, 20, 40, 60, and 80 s. Additionally, measurements of mass, P-wave velocity, and porosity were taken before and after microwave irradiation, followed by uniaxial compression testing using acoustic emission (AE). The results demonstrated that with increasing microwave irradiation duration, the P-wave velocity and mass of shale gradually decreased, exhibiting anisotropic behavior for the P-wave velocity. The highest degree of weakening (24.75%) was observed for shale at 0°, while the lowest (7.03%) occurred at 90°. Both uniaxial compressive strength and elastic modulus weakened gradually. Notably, USC displayed more significant weakening at 30° and 60°. The energy evolution pattern revealed increasing dissipated energy and decreasing brittleness index, enhancing anisotropy with duration, particularly at 60°. Moreover, AE captured the transition of failure mode from tensile splitting to tensile-shear failure. The results indicated the significant and anisotropic weakening effect of microwave irradiation on shale. The increase in porosity and permeability also provided preliminary evidence for the enhanced permeability ability of shale, resulting in reduced hydraulic fracturing pressures and decreased environmental pollution. Furthermore, the changes of most parameters revealed threshold effect of microwave irradiation duration, which can be classified as weak or strong depending on whether it occurs before or after 40 s. Therefore, attention should be paid to the threshold conditions of microwave irradiation duration to achieve efficient extraction. This research provided initial theoretical support for microwave-assisted shale gas exploitation.