Methods In 88 consecutive patients (66.9 ± 9.59 years, 63% men) with varying degrees of aortic valve stenosis, high-resolution 3D SSFP images (3D planimetry; 2.0 mm slice thickness, 20 contiguous slices; image matrix, 256 ‘ 209) were acquired with single breath-hold during mid systole and mid diastole. SSFP cine MR imaging (2D planimetry) and velocity-encoded cine MRI (slice thickness, 4.5 mm) in three levels of aortic valve were also performed. AVA area was measured by two experienced observers using commercial software (iNtuition, TeraRecon). MR imaging measurements and image quality were compared with transthoracic echocardiographic measurements of effective aortic orifices (EAO) using the continuity equation (1 = severe blurring of images, 2 = moderate blurring of valve contours; 3 = mild blurring of valve contours, 4 = excellent and no artifact). Sensitivity for accurate measurement and receiver operating characteristic (ROC) curve were calculated. Intraand interobserver agreements were determined by using intraclass correlation coefficient (ICC). Results Mean AVA derived by 3D planimetry, 2D planimetry, and echocardiography were 0.77 ± 1.04 cm, 0.72 ± 1.16 cm, and 0.75 ± 0.32 cm, respectively. The ICC value of 3D planimetry was higher than 2D planimetry [0.799 (CI, 0.691-0.869) vs. 0.743 (CI, 0.605-0.832)] with echocardiographic EAO as the standard of reference. The grade of image quality of 3D planimetry was superior to 2D planimetry (3.65 ± 0.65 vs. 3.17 ± 0.65). The correlation coefficients of maximum peak velocity on velocity-encoded cine MR imaging with 3D planimetry and that with 2D planimetry were 0.42 (p < 0.05) and 0.35 (p < 0.05). Intraand interobserver agreements for 3D planimetry were excellent [ICC = 0.949 (CI, 881-979) and 0.846 (CI, 0.636-0.935), respectively; both, p = 0.000).