Vegetative Soybean Plants Research Articles

Evaluation of the prospects for the use of growth-stimulating complexes of endometabolites of cyanobacteria Arthrospira platensis in soybean cultivation

For the first time, data were obtained on the use of the cyanobacteria Arthrospira platensis endometabolite complex and its modifications, fortified (additionally enriched) with nutrients B, Se, and P in soybean cultivation technology, which acted not only as growth regulators, but also as adaptogens to external abiotic stress factors during the growing season of the studied crop. It was found that the use of complexes fortified with phosphorus and selenium in pre-sowing seed treatment (0.6 l/t) and foliar top dressing of vegetative soybean plants in the phase of 1-3 triple leaves provides the greatest increase in grain yield by 0.23 and 0.2 t/ha, or by 10.1 and 8.8% in the indeterminate Mezenka variety, and protein collection at the same time is 1022.5 and 1012.7 kg/ha, respectively.

Investigation of nitrogen fixation activity soybeans plants by mineral fertilizers foliar treatment

The results of the study of nitrogen-fixing activity during foliar treatment of vegetative soybean plants with liquid mineral fertilizers containing macro-microelements in various forms in the foothill zone of Kabardino-Balkaria are presented. The object of research is the early ripe soybean variety SK Veda. The soil of the experimental plot is a leached, medium-thick, low-humus, heavy loamy chernozem on carbonate clays, with a humus content in the arable layer of 3.1 %, pHkcl 5.0, mobile phosphorus 7.5 and exchangeable potassium 8.0 mg/100 g of soil. Encrustation of soybean seeds was carried out using a specific film-forming agent based on soybean phosphatides with the inoculant Nitrofix. The results of studies for 2020-2022 are given. with an assessment of the dynamics of the formation of the symbiotic apparatus by the number of nodules (pcs/m2) and by the mass of nodules (g/m2) in ontogeny of the macrosymbiont. The peak of nodule formation in soybean plants of the SK Veda variety fell on the flowering phase. The highest nodulation rates were noted in the control variant: in 2020 - 306 pcs/m2; in 2021 - 408 pcs/m2; in 2022 - 96 pcs/m2. It has been shown that foliar treatment of soybean plants with nutrient deficiency correctors has an impact on nodulation rates, with differences being noted depending on the treatment time. In the variant of Polydon NPK treatment in the primordial leaf phase, the nodulation parameters were minimal in the experiment over the years of the study (86; 216; 56 pcs/m2). Treatment of SK Veda soybean plants in the primordial leaf phase with molybdenum-containing fertilizers ensures the formation of larger nodules. The values of the nodule weight in the flowering phase in the variant of the treatment of Polydon plants with molybdenum in the primordial leaf phase increased by 1.56; 2.09; 1.11 times, respectively, for 2020, 2021, 2022. Nitrogenase activity averaged 26.4-281.6 µg N2/plant.h over the years of the study hour. In the control (inoculation with nitrogen-fixing microorganisms), the activity of nitrogenase was 208.3 µg N2/plant.h. Inoculation of soybean seeds with nitrogen-fixing microorganisms and foliar treatment of plants with Polydon molybdenum increased nitrogenase activity by 35.2-19.1 % and increased protein content by 5.0-3.9 % relative to control, depending on the treatment phase.

Применение инокулянта и микроудобрений на сое в условиях Центрального Черноземья

The effect of soybean seeds inoculation as well as the treatment of seeds and vegetating soybean plants with microfertilizers on productivity and economically valuable traits of the crop in the conditions of the Central Chernozem region was studied. The highest yield – 2.42 t/ha (on average for four years) was obtained on the variant the seed inoculation and the treatment of seeds and vegetative soybean plants with a microfertilizer Microvit St were applied. The yield increase in this variant was 0.68 t/ha or 39.1%. The higher amounts of crude protein in soybean seeds were observed in variants where seeds were treated with an inoculant (29.3–32.3%). The greatest difference compared to the control was noted in the variants: Background + Microvit St (seed treatment) + (Microvit Zn + Microvit B (plant treatment); Background + Microvit St (seed treatment + plant treatment), it was 4.2% and 3.5%, respectively. The highest protein yield in the soybean crop was obtained also on the abovementioned variants – 0.77 t/ha and 0.75 t/ha, respectively. The fat content in soybean seeds did not depend on the treatment with preparations and averaged 25.3%. The usage of innovative preparations allowed reducing the prime cost of seeds by 1.82–5.09 thousand RUR/ton and increasing the level of profitability by 15–51% compared to the control variant. As a result of the application of technological practices, an increase in plants preservation by the end of the growing season was noted, the 1000 seeds weight increased and the quality indicators of soybean seeds improved.

Efficiency of foliar application of liquid mineral fertilizers on soybean crops

The studies were carried out in 2020-2022 in the piedmont zone of the Kabardino-Balkarian Republic on the “Cherek-Kolos” LLC lands. The soil is leached, medium-thick, slightly humus heavy loamy chernozem on carbonate clays. In an experiment to study the effectiveness of the macro-microelements foliar application in different forms on soybean culture, three factors were studied: varieties SK Veda - early-ripening, Rubin - mid-ripening (factor A); treatment of vegetative plants with liquid mineral fertilizers (factor B); processing time (factor C). The maximum values of soybean yield regardless of the variety were achieved when vegetative soybean plants were treated with the nutrient deficiency corrector Polidon Bor. Maximum productivity is achieved when soybean plants are treated in the primordial leaf phase.

The effect of Pulsar herbicide on yield and quality of soybean seeds under the conditions of the Amur River Region

This article provides data of 2017-2019 on the effect of the Pulsar herbicide (a.i. is imazamox) on the yield and quality of seeds of the Kitrossa mid-season soybean variety under the conditions of the Amur River Region. Vegetative plants were sprayed with the Pulsar herbicide in a dose of 0.8 l/ha at the stage of the third ternate leaf. As the result of the studies performed it was found that over three years the use of the Pulsar herbicide on the average led to a decrease in plant survivability before harvesting by 6 % compared to the control. As the result of studying the structure of the crop it was revealed that, on average, over the years of research, the treatment of vegetative soybean plants of the Kitrossa variety with herbicide led to a decrease in the number of pods by 2 pcs/per 1 plant (LSD05 = 1.1 pcs/ per 1 plant), the weight of seeds from 1 plant – by 0.7 g (LSD05 = 0.5 g). Depending on the use of the herbicide under study and prevailing weather conditions, biological yield decreased by an average of 0.3 t/ha during the years of research. The germinating energy in the obtained seeds in the variant with the Pulsar herbicide use decreased by 5 %, the crude protein content – by 0.6 % and oil by 0.39 % compared to the control variant without the herbicide use. There was noted a tendency to increasing the number of abnormally developed germinants by using the studied herbicide.

Quantitative Trait Loci for Antixenosis Resistance to Corn Earworm in Soybean

Insect resistance in soybean, Glycine max (L.) Merr., is inherited quantitatively and has been difficult to incorporate into high‐yielding cultivars. The objectives of this study were to: (i) use restriction fragment length polymorphism (RFLP) markers to identify quantitative trait loci (QTLs) for antixenosis (nonpreference) resistance to corn earworm, Helicoverpa zea Boddie, in soybean plant introductions PI171451 and PI227687, (ii) determine the location of the QTLs on the public soybean genetic linkage map, (iii) determine their relative magnitude and gene action, and (iv) compare them to insect resistance QTLs previously detected in PI229358. RFLP maps were constructed in two insect‐susceptible × insect resistant soybean populations: Cobb × PI171451 and Cobb × P1227687. Insect resistance was measured either in the field or greenhouse as percentage defoliation by corn earworm on vegetative soybean plants. Marker‐QTL associations were detected by interval mapping and confirmed by single‐factor analysis of variance. Comparisons were made between QTLs identified in this study and those identified previously using PI229358 as the source of insect‐resistant germplasm. Among the three resistant genotypes (PI171451, PI227687, and PI229358) a QTL on linkage group (LG) ‘H’ was shared among all three genotypes (accounting for an R2 of 19,9, and 17%, respectively), and a major QTL on LG ‘M’ was shared between PI171451 and PI229358 (R2= 37% for each). A minor QTL on LG ‘C2’ was identified which was unique to PI227687 (R2= 11%) and a minor QTL on LG ‘D1’ was identified which was unique to PI229358 (R2 = 12%). In addition, a QTL was found on LG ‘F’ in the susceptible genotype, Cobb (R2 = 20%). This QTL is in a region of the soybean genome which has been previously associated with a cluster of soybean pathogen‐resistance loci.

Influence of Daily Photosynthetic Photon Flux Density on Foliar Carbon Metabolite Levels in Nitrogen-Limited Soybean Plants

A study was undertaken to examine how the daily photosynthetic photon flux density (PPFD), during prolonged inorganic nitrogen (N) limitation of vegetative soybean plants, influenced the magnitude of leaf photosynthate partitioning into starch and sucrose as well as into anaplerotic products, e.g., phosphoenolpyruvate and malate. Glycine max cv. Williams plants were grown from emergence to preflowering (≈27-32 d) under either N-Sufficient (N-Suff) conditions (14.5 mM NO-3 plus NH+4) or N-limited (N-Lim) conditions (3.0 mM NO-3 plus NH+4). N-Suff and N-Lim plants were grown from emergence in the growth chamber with daily PPFD of 500 or 750 μmol photons m-2 s-1. Measurement of foliar gas exchange revealed that regardless of the PPFD level, net photosynthesis (Pn) rate in mature leaflets of N-Suff plants was 1.2 times greater than those of N-Lim plants. Additionally, Pn rate in N-Suff leaflets, as well as N-Lim leaflets, was 1.4 times greater for plants grown at 750 compared with those grown at 500 μmol photons m-2 s-1. Regardless of the daily PPFD level, foliar stomatal conductance was higher in N-Suff compared with N-Lim leaves, but this did not appear to be a factor influenced by higher light in N-Lim plants. The shoots of N-Suff and N-Lim plants grown in the higher light accumulated ≈1.7 and 1.6 times more dry matter, respectively, than their counterparts grown at lower light energy. Further, both N-Suff and N-Lim plants grown at the higher light energy displayed at least two times higher root mass. In both N-Suff and N-Lim plants, higher daily PPFD, compared with lower PPFD level, resulted not only in higher starch and sucrose accumulation but also in a more rapid daily rise in malate levels and higher malate net accumulation rates. During prolonged N limitation of soybean plants in elevated PPFD, foliar starch and sucrose accumulation was predominant over anaplerotic metabolites. It was concluded that in spite of N limitation, soybean plants growing in elevated PPFD displayed a more active foliar starch and sucrose accumulation as well as a more active anaplerotic anabolism than the lower-PPFD-adapted plants, in part because the higher rate of photosynthesis supplied higher levels of carbon metabolites.

Leaflet photosynthesis rate and carbon metabolite accumulation patterns in nitrogen-limited, vegetative soybean plants

Prolonged inorganic nitrogen (NO3 (-)+NH4 (+)) limitation of non-N2-fixing soybean plants affected leaflet photosynthesis rates, photosynthate accumulation rates and levels, and anaplerotic carbon metabolite levels. Leaflets of nitrogen-limited (N-Lim), 27-31-day-old plants displayed ≈ 15 to 23% lower photosynthesis rates than leaflets of nitrogen-sufficient (N-Suff) plants. In contrast, N-Lim plant leaflets displayed higher sucrose and starch levels and rates of accumulation, as well as higher levels of carbon metabolites associated with sucrose and starch synthesis, e. g., glycerate-3-phosphate and glucose phosphates, than N-Suff plant leaflets. Concurrently, levels of soluble protein, chlorophyll, and anaplerotic metabolites, e.g., malate and phosphoenolpyruvate, were lower in leaflets of N-Lim plants than N-Suff plants, suggesting that the enzymes of the anaplerotic carbon metabolite pathway were lower in activity in N-Lim plant leaflets. Malate net accumulation rates in the earliest part of the illumination period were lower in N-Lim than in N-Suff plant leaflets; however, by the midday period, malate accumulation rate in N-Lim plant leaflets exceeded that in leaflets of N-Suff plants. Further, soluble protein accumulation rates in leaflets of N-Suff and N-Lim plants were similar, and the rate of dark respiration, measured in the early part of the dark period, was higher in N-Lim plant leaflets than in N-Suff plant leaflets. It was concluded that during prolonged N-limitation, foliar metabolite conditions favored the channelling of a large proportion of the carbon assimilate into sucrose and starch, while assimilate flow through the anaplerotic pathway was diminished. However, in some daytime periods, there was a normal level of carbon assimilate channelled through the anaplerotic pathway for ultimate use in amino acid and protein synthesis.

Performance of Southern Green Stink Bug (Heteroptera: Pentatomidae) Nymphs and Adults on a Novel Food Plant (Japanese Privet) and Other Hosts

Japanese privet, Ligustrum japonicum Thunb. (Oleaceae), was found to be a novel food plant of the southern green stink bug, Nezara viridula (L.), in the state of Parana, Brazil. In the laboratory, nymphs performed better when fed on immature fruits of soybean, Glycine max (L.) Merrill (Leguminosae), than on immature fruits of Japanese privet. Female and male nymphs required 6.7 and 4.6 d more to complete development on Japanese privet than on soybean, and had greater mortality on the former (38.7%) than on the latter (20.0%) food plant. At emergence, adult fresh body weight was ≈31-40% greater on soybean than on Japanese privet. In contrast, adult N. viridula performed better on Japanese privet than on soybean; 62.5% of females oviposited on the 1st and 45.0% on the 2nd food. Fecundity was 2-3 times greater on Japanese privet [alone or supplemented with dried soybean seeds + shelled peanuts, Arachis hypogaea (L.) (Leguminosae)] than on soybean fruits or on vegetative soybean plants or water cress, Nasturtium officinale L. (Brassicaceae), leaves. These. 2 last foods were supplemented with dried soybean seeds + peanuts. The amount of fresh body weight gain up to the 4th wk of adult life was ≈3 times greater on Japanese privet fruits than on soybean fruits. Total longevity was similar for females and males on Japanese privet or soybean fruits, but it was greater on Japanese privet + soybean seeds + peanuts than on water cress + soybean seeds + peanuts. These results indicate that N. viridula has expanded its host range to Japanese privet in Brazil, and that the berries of this plant are suitable food particularly for adults.

Effect of Sulfur Nutrition on the Redistribution of Sulfur in Vegetative Soybean Plants.

Soybean (Glycine max L.) plants were grown with sulfate at 2 (S2) or 20 [mu]M (S20) and treated with [35S]sulfate between d 36 and 38. Growth was continued with or without 20 [mu]M sulfate (i.e. S2 -> S0, S2 -> S20, etc.). When the leaves of S20 -> S20 plants were 70% expanded, they exported S and 35S label from the soluble fraction, largely as sulfate, to new expanding leaves. However, 35S label in the insoluble fraction was not remobilized. Very little of the 35S label in the soluble fraction of the leaves of S20 -> S0 plants was redistributed; most was incorporated into the insoluble fraction. The low levels of S remobilization from the insoluble fraction were attributed to the high level of N in the nutrient solution (15 mM). Most of the 35S label in S2 plants at d 38 occurred in the soluble fraction of the roots. In S2 -> S0 plants the 35S label was incorporated into the insoluble fraction of the roots, but in S2 -> S20 plants 35S label was rapidly exported to leaves 3 to 6. It was concluded that the soluble fraction of roots contains a small metabolically active pool of S and another larger pool that is in slow equilibrium with the small pool.

Nodulation and nitrogen fixation on vegetative soybean plants as affected by photoperiod and gibberellic acid

Long-day photoperiods and applications of gibberellic acid promoted shoot growth by stimulating leaf enlargement as a result of increasing avaliable photosynthates, which was also reflected in the higher leaf, stem and nodule plant dry weights. Short-day plants had more nodules, but they were smaller (by weight) than those of long-day plants. Gibberellic acid at 1.5×10(-6) M enhanced nodule growth without preventing nodule formation. Factors other than just gibberellic acid are concluded to be involved in the responses of nodulation and nitrogen fixation to day length.

Regulation of Photosynthetic Carbon Metabolism in Cucumber by Light Intensity and Photosynthetic Period

The effects of photosynthetic periods and light intensity on cucumber (Cucumis sativus L.) carbon exchange rates and photoassimilate partitioning were determined in relation to the activities of galactinol synthase and sucrose-phosphate synthase. Carbon assimilation and partitioning appeared to be controlled by different mechanisms. Carbon exchange rates were influenced by total photon flux density, but were nearly constant over the entire photoperiod for given photoperiod lengths. Length of the photosynthetic periods did influence photoassimilate partitioning. Assimilate export rate was decreased by more than 60% during the latter part of the short photoperiod treatment. This decrease in export rate was associated with a sharp increase in leaf starch acccumulation rate. Results were consistent with the hypothesis that starch accumulation occurs at the expense of export under short photoperiods. Galactinol synthase activities did not appear to influence the partitioning of photoassimilates between starch and transport carbohydrates. Sucrose phosphate synthase activities correlated highly with sugar formation rates (sucrose, raffinose, stachyose + assimilate export rate, r = 0.93, alpha = 0.007). Cucumber leaf sucrose phosphate synthase fluctuated diurnally in a similar pattern to that observed in vegetative soybean plants.

Effect of nodulation on the nitrate assimilation in vegetative soybean plants

Effect of nodulation on the nitrate assimilation in vegetative soybean plants

Association between sucrose-phosphate synthase activity in leaves and plant growth rate in response to altered aerial temperature

Vegetative soybean plants (Glycine max (L.) Merrill, cv. ‘Ransom’) were grown at three aerial temperatures to examine the relationship between sucrose-phosphate synthase (SPS) activities of leaves and whole-plant growth rate. Plans were exposed for 17 days to day/night temperatures of 26/22°C, 22/18°C, or 18/14°C, or 18/14°C, which resulted in a progressive lowering of relative growth rates (0.129, 0.109 and 0.067 g g−1 day–1, respectively). Net CO2 exchange rates (CER), SPS activities and calculated assimilate export rates of the most recently expanded trifoliolate leaf in the canopy were decreased at lower temperatures. The temperature effects were reversible, as CER and SPS activities of selected expanded leaves were increased within 3 days after plants were switched from 18/14°C to 30/26°C and decreased following transfer from 26/22°C to 18/14°C/. The results are interpreted as evidence that changes in the activity of SPS are an integral part of the coordinatedadjustments in the leaf photosynthetic system which limit formation and export of sucrose as the temperature in the growth environment declines.

Effects of canopy defoliation in the dark on the activity of sucrose phosphate synthase

Summary Short-term experiments were conducted with vegetative soybean plants ( Glycine max L. Merrill, cv. ‘Ransom’) to determine whether source-sink manipulations in the dark affected the apparent rate of starch mobilization and the activities of several enzymes involved in sucrose metabolism. When ‘demand’ for sucrose from a particular source leaf was increased in the dark by defoliation of all other source leaves, leaf sucrose-content was reduced initially, but the rate of starch mobilization was unaffected relative to intact controls. Defoliation altered the magnitude of the diurnal rhythm in sucrose phosphate synthase (SPS) activity such that the activity of SPS was markedly greater in defoliated plants relative to the controls. In contrast defoliation had no effect on the activities of sucrose synthase, cytosolic fructose bisphosphatase, and UDP-glucose pyrophosphorylase. The rate of sucrose formation in the dark was apparently limited by the rate of starch mobilization, and could not be increased by short-term changes in the demand for sucrose.

Characterization of Diurnal Changes in Activities of Enzymes Involved in Sucrose Biosynthesis

Experiments were conducted with vegetative soybean plants (Glycine max [L.] Merr., ;Ransom') to determine whether the activities in leaf extracts of key enzymes in sucrose metabolism changed during the daily light/dark cycle. The activity of sucrose-phosphate synthase (SPS) exhibited a distinct diurnal rhythm, whereas the activities of UDP-glucose pyrophosphorylase, cytoplasmic fructose-1,6-bisphosphatase, and sucrose synthase did not. The changes in extractable SPS activity were not related directly to photosynthetic rates or light/dark changes. Hence, it was postulated that the oscillations were under the control of an endogenous clock. During the light period, the activity of SPS was similar to the estimated rate of sucrose formation. In the dark, however, SPS activity declined sharply and then increased even though degradation of starch was linear. The activity of SPS always exceeded the estimated maximum rate of sucrose formation in the dark. Transfer of plants into light during the normal dark period (when SPS activity was low) resulted in increased partitioning of photosynthate into starch compared to partitioning observed during the normal light period. These results were consistent with the hypothesis that SPS activity in situ was a factor regulating the rate of sucrose synthesis and partitioning of fixed carbon between starch and sucrose in the light.

Effects of Main-Stem Girdling of Early Vegetative Stages of Soybean Plants by Threecornered Alfalfa Hoppers (Homoptera: Membracidae)1

Effects of main-stem girdling of early vegetative stage of soybean plants by Spissililus festinus (Say) were studied in small-plot tield tests from 1978 through 1980. Plant responses after girdling were observed and categorized into five main responses. In 2 of the 3 study years, yield reductions occurred when girdling levels were >65%. Possible yield losses from mortality and inability to harvest girdled plants were compensated for by healthy ungirdled and girdled plants that recovered. Based on these data, a damage threshold for main-stem girdling of early vegetative soybean plants by S. festinus was established for Arkansas.

Changes in Starch Formation and Activities of Sucrose Phosphate Synthase and Cytoplasmic Fructose-1,6-bisphosphatase in Response to Source-Sink Alterations

Short term experiments were conducted with vegetative soybean plants (Glycine max L. Merr. ;Ransom' or ;Arksoy') to determine whether sourcesink manipulations, which rapidly changed the ;demand' for sucrose and partitioning of photosynthetically fixed carbon into starch, were associated with alterations in activities of sucrose-P synthase and/or cytoplasmic fructose-1,6-bisphosphatase in leaf extracts. When demand for sucrose from a particular source leaf was increased by defoliation of other source leaves, starch accumulation was restricted and activities of both enzymes were markedly enhanced. When demand for sucrose from source leaves was limited by excision, starch accumulation in the detached leaves was increased while activity of sucrose-P synthase declined sharply. The consistent responsiveness of sucrose-P synthase activity to changes in demand for sucrose supports the contention that regulation of sucrose-P synthase is an integral component of the system which controls sucrose biosynthesis and partitioning of carbon between starch and sucrose biosynthesis in the light.

Water Stress Effects on CO2 Assimilation, Photosynthate Partitioning, Stomatal Resistance, and Nodule Activity in Soybean1

14CO2 assimilation, photosynthate distribution, stomatal resistance, and acetylene reduction activity of vegetative soybean plants [Glycine max (L.) Merr., var. ‘Clay’] were examined 3 hours after pulse labeling the leaves with 14CO2. The plants had previously been water stressed for 20 hours by polyethylene glycol additions to the culture solution. Photosynthetic CO2 exchange rate (CER) and acytelene reduction activity of control and water stressed soybean plants were also monitored for 6 hours following polyethylene glycol induced water stress.14CO2 assimilation and specific nodule activity (SNA) decreased and stomatal resistance (r8) increased with increasing water stress in the 14CO2 labeling experiment. There was a significant negative correlation (r=−0.71) between SNA and r8• Dry weights of leaves, of stems plus petioles, and of nodules were significantly decreased in the water stress treatments, while root dry weights increased. Water stress resulted in a redistribution of 14C with a greater proportion of the 14C being translocated to the roots and nodules at the expense of the leaves.In the time course study, CER decreased after the imposition of an abrupt water stress (−12 bar), but total nodule activity was not affected. It is suggested that in. hibition of nodule activity under long term water stress was not caused solely by the decreased CER, but also by changes in photosynthate pool sizes.

THE USE OF BIOLOGICALLY ACTIVE SUBSTANCES TO INCREASE THE RESISTANCE OF SOYBEANS TO HERBICIDES

The research was carried out of the effect of a biologically active substance on the reduction of herbicidal load during the cultivation of Kitrossa soybean variety in the Amur Region.As a biologically active substance used the natural preparation ExtraCor obtained by processing Daurian larch (Larix gmelinii).Field experiments were carried out in 2017–2019 on meadow chernozem-like soils in the southern soil-climatic zone of the Amur Region, laboratory experiments were carried out in the seed science group of the All-Russian Soybean Research Institute.Laboratory investigation found that on average for three years the use of the biologically active substance ExtraCor allowed to reduce the number of abnormally developed seedlings by 2.3 times compared with the control.The length of germinating seedlings during ExtraCor seed treatment was, on average, 1.5 cm longer relative to the control. The variation range was 17.3%, in the control - 18.4%.The treatment of seeds and vegetative soybean plants with the ExtraCor drug contributed to an increase in germination energy by 3...6% compared with the control variant and by 8...11% relative to the use of the herbicide. In the variant with the use of herbicidal treatment, a 5% decrease in the germination energy was noted in comparison with the control variant. The analysis of the crop structure showed that on average over the years of research, the use of a natural preparation for vegetating plants in combination with presowing seed treatment contributed to an increase in the number of beans by 0.4-2.5 pcs., and seeds - by 0.7-3.5 pcs. per 1 plant compared to the variant where, when processing crops with herbicides, ExtraCor was not used. The weight of seeds from 1 plant increased by 0.2-0.8 g. The biological yield of seeds on average increased to 3.08 t/ha with 2.92 t/ha in the control and 2.62 t/ha in the variant with the use of herbicides.