Eucalyptus plantations are one of the primary artificial forests in southern China, experiencing rapid expansion in recent years due to their significant socio-economic benefits. This expansion has raised concerns about the ecological environment, necessitating accurate mapping of eucalyptus plantations. In this study, the phenological characteristics of eucalyptus plantations were utilized as the primary classification basis. Long-term time series Landsat and Sentinel-2 data from 2000 to 2022 were rigorously preprocessed pixel by pixel using the Google Earth Engine (GEE) platform to obtain high-quality observation data. The Continuous Change Detection and Classification (CCDC) algorithm was employed to fit the multi-year observation data with harmonic curves, utilizing parameters such as normalized intercept, slope, phase, and amplitude of the fitted curves to characterize the phenological features of vegetation. A total of 127 phenological indices were generated using the Normalized Burn Ratio (NBR), Normalized Difference Fractional Index (NDFI), and six spectral bands, with the top 20 contributing indices selected as input variables for the random forest algorithm to obtain preliminary classification results. Subsequently, eucalyptus plantation rotation features and the Simple Non-Iterative Clustering (SNIC) superpixel segmentation algorithm were employed to filter the results, enhancing the accuracy of the identification results. The producer’s accuracy, user’s accuracy, and overall accuracy of the eucalyptus plantation map for the year 2020 were found to be 96.67%, 89.23%, and 95.83%, respectively, with a total area accuracy of 94.39%. Accurate mapping of eucalyptus plantations provides essential information and evidence for ecological environment protection and the formulation of carbon-neutral strategies.